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ANALISIS PEWARNA ALAMI EKSTRAK AQUADES ANGKAK MERAK PADA PENGAMATAN TELUR CACING SOIL TRANSMITTED HELMINTH Munawaroh, Siti; Shofi, Muh.; Febriansyah, Mukhamad Prima
Jurnal Wiyata Penelitian Sains dan Kesehatan Vol 12 No 1 (2025)
Publisher : LP2M IIK (Lembaga Penelitian dan Pengabdian Masyarakat Institut Ilmu Kesehatan) Bhakti Wiy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56710/wiyata.v12i1.965

Abstract

Latar Belakang: Kecacingan adalah infeksi yang disebabkan oleh berbagai jenis cacing parasit di usus. Spesies ini termasuk dalam kategori Soil Transmitted Helminth (STH) karena perkembangan telur atau larva spesies tersebut membutuhkan tanah untuk berkembang menjadi bentuk yang infektif. Metode paling sederhana untuk memeriksa telur cacing Nematoda Usus adalah metode natif. Metode ini menggunakan reagen Eosin 2% untuk mengamati berbagai komponen pemeriksaan pada preparat atau sediaan. Dibutuhkan pewarnaan alternatif yang lebih ramah lingkungan untuk menggantikan eosin karena sifatnya yang tidak mudah terurai dan menghasilkan limbah yang berbahaya. Salah pewarna telur cacing STH yaitu angkak merah. Pewarna angkak merah memiliki zat berupa antosianin yang mampu mewarnai telur cacing STH. Tujuan dari penelitian ini adalah untuk mengetahui apakah ekstrak aquades angkak merah dapat berfungsi sebagai zat warna untuk mendeteksi infeksi SHT dalam pemeriksaan feses. Metode : Penelitian ini menggunakan eksperimen laboratorium dengan karakteristik deskriptif kategorikal, dengan memvariasikan konsentrasi ekstrak angkak merah (murni, 1:1, 1:2, dan 1:3) dan pH Pewarna (asam dan basa). Hasil : Hasil penelitian menunjukkan bahwa pewarna yang paling efektif yaitu ekstrak angkak merah dengan perbandingan 1:3 dengan pH asam berfungsi sebagai pengganti yang efektif untuk reagen Eosin 2% dalam pewarnaan telur cacing STH. Kesimpulan : Hal ini dapat disimpulkan bahwa ekstrak aquades angkak merah dapat digunakan sebagai pewarna alami untuk mengidentifikasi telur cacing STH.
Modifikasi Pewarna Alami Ekstrak Etanol Sappan Lignum pada Pewarnaan Soil Transmitted Helminth Pengganti Eosin 2% Munawaroh, Siti; Shofi, Muh.
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9245

Abstract

Worms are a disease that attacks humans in tropical areas. The cause of worm infections in the community is from the Soil Transmitted Helminth (STH) worm group, but the government pays little attention to this. In the process of identifying worm eggs, 2% eosin has been used, which apparently produces waste that is difficult to decompose. Sappan lignum is a natural dye because it contains brizilin which can give a red color, so it can be used as a worm egg dye replacing 2% eosin. This study aims to test whether sappan lignum extract can be used as a dye in fecal examination to detect SHT infections. This research is descriptive research. Examination of worm eggs using the direct feces method was carried out using sappan lignum extract dye with 96% ethanol and 2% eosin as a positive control. This research shows that sappan lignum ethanol extract at a coloring ratio of 1:3 is the best dye compared to other ratios and can be used as a substitute for eosin dye. The conclusion of this research is that sappan lignum ethanol extract can be used as a natural dye in identifying worm eggs.
Uji Aktivitas Sel Kanker Payudara T47D Ekstrak Etil Asetat Daun Glodokan Tiang (Polyalthia longifolia L.) Secara In Vitro Shofi, Muh.; Munawaroh, Siti
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9345

Abstract

Cancer is one of the main causes of death throughout the world. Breast cancer is a non-communicable disease and a type of cancer that is often suffered by women. Breast cancer is a reproductive health problem, both in the world and in Indonesia, which is now a serious concern. Medical treatment of cancer is usually carried out with chemotherapy, surgery and radiotherapy, but the Indonesian people are familiar with and use efficacious plants as an effort to cure various diseases, including cancer. One of them is by using glodokan mast leaves or Polyalthia longifolia L. as natural chemotherapy from biological ingredients. This plant is reported to be high in antioxidant content, so it can be used as an anticancer agent, especially T47D breast cancer. The aim of this research is to examine the compound content and activity of T47D breast cancer cells from P. longifolia leaf extract. This research is basic experimental research, namely by comparing the IC50 value of ethyl acetate extract on young and old leaves. Based on the research results, it is known that the ethyl acetate extract in young and old leaves contains alkaloids, flavonoids, tannins and terpenoids which are useful as anticancer compounds. Cytotoxic activity of ethyl acetate extract of P. longifolia leaves against T47D breast cancer cells with an IC50 value of 253.76 μg/mL on old leaves and young leaves of 220.54 μg/mL with moderate cytotoxicity.
Peningkatan Pengetahuan Masyarakat Desa Sidomulyo Kecamatan Semen Kabupaten Kediri tentang Bunga Rosella (Hibiscus sabdariffa) sebagai Minuman Kesehatan: Sebuah Studi Intervensi Edukasi Shofi, Muh.; Munawaroh, Siti; Mu'arofah, Binti; Ponnaiah, Paulraj
Journal of Community Engagement and Empowerment Vol. 6 No. 2 (2024)
Publisher : Institut Ilmu Kesehatah Bhakti Wiyata Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Diabetes mellitus merupakan salah satu penyakit tidak menular yang cukup serius. Penyakit ini ditandai dengan adanya insulin yang tidak dapat diproduksi secara maksimal oleh pankreas yang mempu mengatur konsentrasi glukosa dalam darah. Tanda-tanda terjangkitnya penyakit diabetes mellitus yaitu sering mengeluarkan urine dalam jumlah banyak, sering merasa haus dan lapar, serta badan terasa lemas. Tingginya angka kejadian penyakit ini perlu adanya suatu tindakan untuk menanggulangi penyakit ini. Salah cara penanggulangan penyakit ini yaitu dengan cara pembuatan teh herbal kelopak bunga rosella. Sebab tanaman ini memiliki kandungan senyawa aktif yang dapat menanggulangi penyakit diabetes. Tujuan kegiatan pengabdian masyarakat ini yaitu untuk memberikan pengetahuan dan keterampilan kepada masyarakat Desa Desa Sidomulyo Kecamatan Semen Kabupaten Kediri sehingga dapat meningkatkan pengetahuan terhadap penanggulangan penyakit diabetes millitus dengan menggunakan minuman herbal teh bunga rosella. Metode pelaksanaan pengabdian masyarakat kali ini yaitu dengan membentuk forum group discussion (FGD), pemberian pretest dan postest tentang materi pemanfaatan kelopak bunga rosella yang akan diberikan serta monitoring setelah kegiatan pengabdian masyarakat. Berdasarkan hasil pretest dan postest yang dibagikan kepada para peserta diketahui bahwa tingkat pengetahuan mitra mengenai pemanfaatan kelopak bunga rosella mengalami peningkatan. Hal tersebut terbukti dari hasil pretest menunjukan nilai pengetahuan mitra hanya 46.7%. Adanya hal tersebut dapat dikatakan nilai pengetahuan peserta atau mitra pengabdian tergolong rendah. Setelah peserta mendapatkan materi dan pelatihan dari narasumber didapatkan berupa uji postest mengalami kenaikan pengetahuan tentang pemanfaatan kelopak bunga rosella sebesar 100%. Hal ini menunjukan bahwa tingkat pengetahuan dan upaya sikap positif peserta semakin meningkat.
Analysis of Mercury Concentration in Food Crops Around the Lebong Regency Gold Mine Fatimatuzzahra, Fatimatuzzahra; Sri Astuti, RR; Satriawan, Dedi; Sipriyadi, Sipriyadi; Istikhomah, Sekar; Eva Nadya Purba, Juwita; Farid Fadhlullah, Muhammad; Wahyu Nengsih, Anisa; Shofi, Muh.; Saputra, Alanindra
Biosfer: Jurnal Tadris Biologi Vol 16 No 2 (2025): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v16i2.29163

Abstract

This study aims to inventory the types of food crops growing around tailings ponds and to quantify mercury concentrations in food crop tissues growing in the area around the gold mine. The samples taken are plants consumed by residents. Mercury content testing uses a mercury analyzer. The results of the study showed that from 4 observation locations, a total of 11 types of food crops were found, all of which contained mercury (Hg) with a concentration range of 0.01-0.54 mg/kg. Similarly, the soil as a growing medium contains quite high levels of mercury at location 1, namely 54.36 mg/kg, and the lowest at location 3 at 0.12 mg/kg. The concentration of mercury in vegetable leaves is much higher than in other organs such as tubers, flowers, and fruits. Furthermore, to plant plants for consumption, it is best to pay attention to the condition of the growing medium, as a prevention of heavy metal (mercury) contamination, such as the environment (air, soil, water) that is far from waste retention ponds or waste discharge streams containing heavy metals (mercury). Analisis Konsentrasi Merkuri pada Tanaman Pangan di Sekitar Tambang Emas Kabupaten Lebong ABSTRAK: Penelitian ini bertujuan untuk menginventarisasi jenis tanaman pangan yang tumbuh disekitar kolam penampungan limbah (tailing) dan untuk mengkuantifikasi konsentrasi merkuri dalam jaringan tanaman pangan yang tumbuh di area sekitar tambang emas. Sampel yang diambil merupakan tanaman yang memang dikonsumsi oleh warga setempat. Uji kandungan merkuri menggunakan mercury analyzer. Hasil penelitian menunjukkan dari 4 lokasi pengamatan, ditemukkan Sebanyak 11 jenis tanaman pangan yang semuanya mengandung merkuri (Hg) dengan kisaran konsentrasi 0,01-0,11 mg/kg. Demikian pula, tanah sebagai media tanam mengandung kadar merkuri yang cukup tinggi di lokasi 1, yaitu 54,36 mg/kg, dan paling rendah dilokasi 3 sebesar 0,12 mg/kg. konsentrasi merkuri dalam daun sayuran jauh lebih tinggi dibandingkan organ lain seperti tuber, bunga, dan buah. Selanjutnya, untuk menanam tanaman yang akan dikonsumsi sebaiknya memperhatikan kondisi media tumbuh, sebagai pencegahan dari kontaminasi logam berat (merkuri), seperti lingkungan (udara, tanah,air) yang jauh dari kolam penampungan limbah maupun aliran pembuangan limbah yang mengandung merkuri.
Edukasi Pemanfaatan Herbal (Daun Sirih, Cengkeh dan Kayu Manis) Sebagai Obat Kumur Untuk Mencegah Caries Gigi Di Kawasan Gelandangan Chow Kit Malaysia Aryantini, Dyah; Shofi, Muh.; Agustina, Lia; Yuliati, Ninis; Fauzi Sabban, Indra
Bersama : Jurnal Pengabdian Kepada Masyarakat Vol. 4 No. 1 (2026): Bersama: Jurnal Pengabdian Kepada Masyarakat
Publisher : Fakultas Pertania Universitas Muara Bungo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36355/bsm.v4i1.67

Abstract

The Chow Kit settlement in Kuala Lumpur, Malaysia, is characterized by a population of homeless and low-to-no-income residents. As such, this area frequently becomes a focus for social institutions implementing Community Service Responsibility (CSR) programs, particularly in the health domain. This CSR initiative represents a collaborative effort between USIM Malaysia (Faculty of Dentistry) and the Faculty of Pharmacy (IIK Bhakti Wiyata Kediri, Indonesia), focusing on educational interventions to prevent and reduce dental caries through the use of herbal mouthwash formulations. Dental caries has emerged as a prevalent oral health concern with a significant impact on overall health. The condition originates from the formation of Streptococcus mutans bacterial biofilms, which are typically exacerbated by excessive consumption of sugary foods and tobacco use. The community service methodology employed participatory counseling and educational approaches, utilizing purposive sampling techniques. Evaluation of the program demonstrated enhanced comprehension among the 22 participants, with mean pre-test scores of 57.39 improving to 79.09 post-intervention, reflecting a 21.7% increase. While some participants maintained baseline scores, 86.5% of attendees (19 individuals) achieved improvements exceeding 20 points. Sustainable monitoring and evaluation mechanisms remain essential, given that the participant cohort primarily consists of homeless individuals whose transient living circumstances often preclude consistent maintenance of personal oral hygiene practices.
Edukasi dan Pemeriksaan Pediculus Humanus Capitis Pada Siswa TK Kusuma Mulya 12 Desa Sendang Kecamatan Banyakan Kabupaten Kediri Shofi, Muh.; Munawaroh, Siti; Purnamasari, Vivien Dwi; Agusti Lukis, Prima
Bersama : Jurnal Pengabdian Kepada Masyarakat Vol. 4 No. 1 (2026): Bersama: Jurnal Pengabdian Kepada Masyarakat
Publisher : Fakultas Pertania Universitas Muara Bungo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36355/bsm.v4i1.72

Abstract

Health problems in school-age children continue to be a major concern due to their impact on health quality and future development. Pediculosis capitis (head lice infestation) is a frequently encountered issue, strongly associated with inadequate personal hygiene, especially hair care. This condition, common in early childhood, causes intense itching, discomfort, and interferes with learning. Therefore, preventive measures through health education and direct screening are needed. This community service initiative aimed to improve students' knowledge of pediculosis capitis and identify infestation rates among students at Kusuma Mulya 12 Kindergarten, Sendang Village, Banyakan District, Kediri Regency. Methods included educational lectures, discussions, demonstrations, and direct scalp examinations. Evaluation utilized pretests and posttests to measure knowledge change, alongside visual inspection to determine infestation prevalence. Conducted in May 2024 with 50 students, the activity revealed low baseline knowledge. Post-intervention, a significant knowledge increase was achieved (95% posttest score). Screening identified a 36% infestation rate. In conclusion, combined education and examination effectively improved knowledge and enabled early detection of head lice. This program is expected to promote early adoption of clean and healthy living habits.