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All Journal HAYATI Journal of Biosciences Bioma : Berkala Ilmiah Biologi JURNAL ANATOMI FISIOLOGI Agrikultura Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Global Medical and Health Communication Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology Bioedukasi: Jurnal Pendidikan Biologi Jurnal Entomologi Indonesia Jurnal Biomedika Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
R.C. Hidayat Soesilohadi
Faculty Of Biology, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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In Silico Analysis of C-Type Lectins as Co-Infection Receptors of Dengue and Chikungunya Viruses in Aedes aegypti Sazali, Munawir; Soesilohadi, R. C. Hidayat; Wijayanti, Nastiti; Wibawa, Tri; Ansori, Arif Nur Muhammad
Makara Journal of Science Vol. 28, No. 1
Publisher : UI Scholars Hub

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Abstract

Aedes aegypti is a primer vector of dengue virus (DENV) and chikungunya virus (CHIKV). The susceptibility of mosquitoes to DENV and CHIKV depends on their recognition receptor of pathogens. C-type lectins (CTLs) are an important mediator of virus infection in A. aegypti. This study aims to identify potential receptors and determine the binding affinity between ligand–receptor interaction, CTLs and virus envelopes (DENV-1, 2, 3, and 4 and CHIKV) interaction based on in silico analysis. Sample sequences were obtained from GenBank (NCBI), and 10 CTLs were acquired from VectorBase. Homology modeling based on a minimum standard of 20% was processed using the SWISS-MODEL server. Molecule interaction was visualized as tertiary protein structure in PyMol V1.7.4. Homology modeling revealed all 3D protein model scores to be at 20%. The highest homology modeling score of 98.72% is obtained for CHIKV envelope protein (KF925315.1). The CTL GenBank ID: AAEL014382-PA has the lowest score at −1407.20 kcal/mol. The predicted mechanism of DENV-1, 2, 3, and 4 co-infections with CHIKV-1 is through the binding of the viral envelope to the CTL. Interaction analysis showed that the viruses enter through an endocytic mechanism. The results of homology modeling and lowest interaction energy could serve as the basis for in vivo studies on virus co-infection in A. aegypti. This information illustrates that CTL receptors facilitate DENV and CHIKV co-infection in A. aegypti.
Pengaruh frekuensi ultrasonik terhadap mortalitas serangga perusak kayu Dinoderus minutus Fabricius (Coleoptera: Bostrichidae) Susanti, Etik; Wiranto, Aryo Seto Pandu; Ismanto, Agus; Soesilohadi, RC Hidayat
Jurnal Entomologi Indonesia Vol 19 No 1 (2022): Maret
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.19.1.55

Abstract

Dinoderus minutus Fabricius (Coleoptera: Bostrichidae) is one of the main pests of wood and bamboo borer. Control of these insects is still dependent on the use of insecticides. This study is a preliminary test for the use of ultrasound frequency as a pest control agent. The research aims to study the effect of ultrasonic frequencies on D. minutus mortality. The method used exposure of ultrasonic frequency to imago D. minutus for 7 days and 14 days indoors with temperatures of 28 °C to 29 °C and humidity of 70% to 81%. Significant results for the ultrasonic frequency treatment which resulted in a D. minutus mortality of 80.00% with a duration of 14 days exposure. The results show that ultrasound frequency with the exact durations can be used as a form of D. minutus control.
The Diversity of Ants (Hymenoptera: Formicidae) on Industrial Forest in Sungai Merah Village, Sarolangun, Jambi with Its Identification Key Fitriyani, Nur Laras; Soesilohadi, RC. Hidayat; Purwanto, Hari
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 3 (2024): September
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.86022

Abstract

Ants have a very important role in an ecosystem. The insects act as decomposers, pollinators, soil aerators, pest controllers, and predators. Their role is very varied so that they can be easily found in various ecosystems, one of which is the rubber and oil palm ecosystem. The ant’s diversity in this forest eventually will affect the productivity of the land. The aim of this study is to determine the diversity and role of ants in the rubber and oil palm plantations in Sungai Merah Village. Both of the plantations dominate the industrial forest in Jambi. The method used in this study was purposive random sampling. Observation plots were installed in the ecosystem of rubber and oil palm plantations; each ecosystem had 4 plots consisting of 9 units of pitfall traps, and 9 units of bait traps. The results of this study show that 15 species of ants are found in the ecosystem of rubber and oil palm plantations. Furthermore, the collected ants consisted of 39.972 individual ants belonging to 12 genera and 5 subfamilies. Ants establish in the ecosystem of rubber and oil palm plantations in Sungai Merah Village have an important role either as predators (Crematogaster spp., Odontomachus rixosus, Odontoponera tranversa, Pheidole huberi, Tetraponera rufonigra, Tapinoma melanocephalum, Camponotus spp. and Colobopsis moeschi), or foragers such as ants from the genera Anoplolepis, Camponotus, Monomorium and Polyrhachis. As predators, Oecophylla smaragdina, Camponotus spp. and Crematogaster spp. also play a role as biological control agents in the ecosystem. 
Comparing Effectiveness of Hand Pollination, Wild Insects and Local Stingless Bees (Tetragonula laeviceps) for Pollination of Exotic Mauritius Raspberry (Rubus rosifolius) Putra, Ramadhani; Dewangga, Rezha Tanu; Hermawan, Endang; Kinasih, Ida; Raffiudin, Rika; Soesilohadi, RC Hidayat; Purnobasuki, Hery
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1050-1060

Abstract

Mauritius raspberry (Rubus rosifolius) is an exotic plant cultivated in Indonesia. Studies showed that the productivity of exotic plants is reducing due to a lack of pollination agent for their origin region. Several methods could be applied to overcome this problem. The study aimed to find the most effective method for pollinating this plant among four pollination regimes: Self-pollination, wild insects, stingless bees (Tetragonula laeviceps), and hand-pollination. The observation was conducted on the insect pollinators' activities (visitation rate, flower constancy, and flower handling time), pollination efficiency, and quality of the fruits produced (fruit volume). The results showed a higher visitation rate (10 to 70 per hour), higher flower constancy (visited from 07.00 to 16.00), and longer flower handling time (13.6 s) of T. laeviceps on raspberry flowers than other insects. High activities related to better raspberry pollination success (96%) and bigger fruits produced. Based on this study, applying stingless bees as pollination agents for exotic plants was the best and potentially applicable to other exotic crops.
Deteksi Dini di Lingkungan Rumah yang Tidak Terawat Sebagai Reservoir Aedes spp. di Perumahan Kota Banjarbaru Muhamat, M; Soesilohadi, RC. Hidayat; Hadisusanto, Suwarno; Umniyati, Sitti Rahmah
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2020: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (570.372 KB)

Abstract

Pembangunan perumahan di perkotaan saat ini tumbuh sangat cepat termasuk di Kota Banjarbaru Ibukota provinsi Kalimantan Selatan. Namun demikian diperumahan tersebut ada beberapa rumah yang tidak dihuni dan tidak dirawat yang berpotensi menjadi habitat nyamuk Aedes spp. Penelitian ini dilakukan untuk menjelaskan lingkungan kebun dan rumah yang tidak terawat sebagai habitat nyamuk Aedes spp. di musim penghujan. Metode penelitian dengan metode purposive random sampling dengan mempertimbangkan perumahan yang ada rumah kosong yang tidak terawat dan diperumahan tersebut pernah terjadi kasus Demam Berdarah Dengue. Lokasi yang terpilih sebagai tempat pengambilan sampel adalah :-3.461389, 114.8503, -3.458056, 114.8456; -3.443056, 114.8586, -3.463611, 114.7731 dan -3.449167, 114.7814. Metode pengambilan sampel dengan memasang ovitrap yang diisi air rendaman jerami 10%. Ovitrap ditaruh stik kayu sebagai tempat bertelurnya nyamuk. Ovitrap ditaruh di bawah kanopi pohon yang terlindung hujan. Ovitrap diamati setiap 2 hari sekali. Hasil penelitian diperoleh telur sebanyak 207 butir. Setiap lokasi juga dilakukan inventarisasi vegetasi (luas kanopi, jumlah tumbuhan, tumbuhan penghasil senyawa repellen nyamuk, tumbuhan bercelah, dan tumbuhan berbunga) dan pengukuran suhu, kelembapan udara, dan pH air. Hasil identifikasi spesies, semua telur berasal dari nyamuk Aedes albopictus. Analisis ANOVA menunjukkan bahwa lokasi -3.461389, 114.8503 dan -3.443056, 114.8586 diperoleh telur yang lebih banyak dibandingkan tiga lokasi yang lainnya. Analisis korelasi antara faktor lingkungan dengan jumlah telur yang diperoleh menunjukkan bahwa faktor luas kanopi tumbuhan mempunyai hubungan yang kuat dan pH air, sedangkan tumbuhan penghasil senyawa repellen nyamuk dan tumbuhan berbunga berkorelasi sedang. Kesimpulan dari penelitian adalah rumah kosong yang tidak terawat dan ditumbuhi tumbuhan dengan kanopi yang lebar di lingkungan perumahan dapat menjadi reservoir nyamuk Aedes spp. terutama Ae. albopictus.
Pengaruh frekuensi ultrasonik terhadap mortalitas serangga perusak kayu Dinoderus minutus Fabricius (Coleoptera: Bostrichidae) Susanti, Etik; Wiranto, Aryo Seto Pandu; Ismanto, Agus; Soesilohadi, RC Hidayat
Jurnal Entomologi Indonesia Vol 19 No 1 (2022): Maret
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.19.1.55

Abstract

Dinoderus minutus Fabricius (Coleoptera: Bostrichidae) is one of the main pests of wood and bamboo borer. Control of these insects is still dependent on the use of insecticides. This study is a preliminary test for the use of ultrasound frequency as a pest control agent. The research aims to study the effect of ultrasonic frequencies on D. minutus mortality. The method used exposure of ultrasonic frequency to imago D. minutus for 7 days and 14 days indoors with temperatures of 28 °C to 29 °C and humidity of 70% to 81%. Significant results for the ultrasonic frequency treatment which resulted in a D. minutus mortality of 80.00% with a duration of 14 days exposure. The results show that ultrasound frequency with the exact durations can be used as a form of D. minutus control.
Co-Authors Adi Sofyan Ansori, Muhammad Agus Ismanto Ali Agus Amelia Nugrahaningrum Andi Gita Maulidyah Indraswari Suhri Andiyatu Andiyatu Arman Wijonarko Aryo Seto Pandu Bellia Alda Ayu Prasetya Budi Mulyaningsih Dewangga, Rezha Tanu Edhi Martono Edhi Martono Entun Santosa Entun Santosa Etik Susanti, Etik Farid Kuswantoro Fitriyani, Nur Laras FX Wagiman Hanna Kawulur, Hanna Hanna S.I. Kawulur, Hanna S.I. Hari Purwanto Hari Purwanto Hermawan, Endang Hery Purnobasuki Ida Kinasih Ign. Joko Suyono Ignatius Sudaryadi Jesmandt Situmorang Jesmandt Situmorang Luciana Djaya Luciana Djaya, Luciana Martua Suhunan Sianipar Mey Priandi Bangun, Mey Priandi Mochamad Ardiansyah Mochamad Hadi Muhamat, M Munawir Sazali Nastiti Wijayanti Niken Satuti Nur Handayani Nur Apriatun Nafisah Purwoko, Agus Putra, Ramadhani Raden Roro Upiek Ngesti Wibawaning Astuti Raden Wisnu Nurcahyo Rahmat Budi Nugroho, Rahmat Budi Ramadhani Eka Putra Rarastoeti Pratiwi RIKA RAFFIUDIN Sih Kahono Siti Sumarmi Sitti Rahmah Umniyati Sukarti Moeljopawiro Sukirno Sukirno SULFIANTO ALIAS Suparmin Suparmin SUWARNO HADISUSANTO Tri Wibawa Udi Tarwotjo, Udi W Darajat Natawigena, W Darajat Wahyu Daradjat Natawigena Wiranto, Aryo Seto Pandu Y. Andi Trisyono Yayuk Rahayuningsih Yayuk Rahayuningsih Soehardjono, Yayuk Rahayuningsih