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All Journal Medicina E-Jurnal Medika Udayana Jurnal Veteriner e-CliniC Medical Journal of Indonesia JBN (Jurnal Bedah Nasional) ISM (Intisari Sains Medis) : Jurnal Kedokteran Majalah Patologi Indonesia Jurnal Respirasi (JR) Neurologico Spinale Medico Chirurgico Jurnal Kedokteran Universitas Palangka Raya Indonesian Journal of Cancer Journal of Global Pharma Technology
Ni Putu Sriwidyani
Departemen Patologi Anatomi Fakultas Kedokteran Universitas Udayana
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Veterinary

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Journal : Jurnal Veteriner

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Growth Hormone Menurunkan Ekspresi Protein p53 dan p21 Sel Endotel Tikus Jantan (GROWTH HORMONE REDUCES P53 AND P21 ENDOTHELIAL PROTEIN EXPRESSION IN MALE RATS) I Gusti Ayu Dewi Ratnayanti; Ni Putu Sriwidyani; I Dewa Ayu Inten Primayanti; I Gusti Kamasan; Nyoman Arijana; I Gusti Nyoman Sri Wiryawan; Ida Ayu Ika Wahyuniari; I Wayan Sugiritama; I Gusti Ngurah Mayun
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The use of growth hormone (GH) treatment in aging related condition such as atherosclerosis is stillcontroversial. Previous study showed GH reduce atherosclerotic plaque and prevent endothelial cellsenescence. This study was aimed to understand the mechanism of GH effect to endothelial senescencethrough p53/p21 pathway. A randomized posttest only control group design study was conducted. Twentymale Wistar rats were randomized into five groups; negative control (P0), positive control (P1), and GHtreated group (P2, P3, P4). Negative control group was fed with standard diet, and others were fed withatherogenic diet for 20 weeks. After 10 weeks, subjects were injected subcutaneously (0,1 mL) with aquadest(P0 and P1) and increasing dose of GH (0,02 IU, 0,04 IU, and 0,08 IU) for P2, P3, P4 once a day respectivelyfor 10 weeks. In the end of the study all subjects were examined for p53 and p21 endothelial proteinexpressions. Immunohistochemistry of endothelial p53 showed reduce expression in treated groups (P0:7.28 ± 0.36; P1: 39.51 ± 1.18; P2: 32.70 ± 1.10; P3: 16.98 ± 0.78; and P4: 14.29 ± 0.38). The reduction was also observed in p21 expression (P0: 5.38 ± 0.49; P1: 37.81 ± 0.76; P2: 26.02 ± 1.54; P3: 16.37 ± 1.24; andP4: 4.82 ± 0.61. One way analysis of variance and post hoc test (LSD) analysis showed significant differencesbetween all groups (p<0.05). In conclusion, GH reduces endothelial expression of p53 and p21 and thispathway may contribute to GH effect on atherosclerotic plaque and endothelial senescence.
Co-Authors Anak Agung Ayu Ngurah Susraini Angelia Ongko Prabowo annie minerva datui Astuti, Ida Ayu Jelantik Bintang Dwi Oktaviani Cahyani, Kadek Cindy Dwi Citra, Dandy Damayanti, Ni Kadek Ayu Maya Desak Made Cittarasmi Saraswati Seputra Dewi, Ida Ayu Krisna Cantika Dewi, Ida Ayu Meilasari Dewi, Ni Putu Wulan Tantri Diahningrum, Sylvi Handoko, Jovi Carina Henky, . Herlina Eka Shinta Herman Saputra Herman Saputra Herman Saputra Herman Saputra Herman Saputra Herman Saputra Herman Saputra I Dewa Ayu Inten Primayanti I Gde Raka Widiana I Gede Indradika Pratama Putra I Gusti Ayu Dewi Ratnayanti I Gusti Ayu Sri Mahendra Dewi I Gusti Kamasan I Gusti Kamasan Arijana I Gusti Kamasan Nyoman Arijana I Gusti Ngurah Kade Mahardika I Gusti Ngurah Made Cesar Vajrashrava Sunantara I Gusti Ngurah Mayun I Gusti Ngurah Pratama Yuda Atmaja I Gusti Nyoman Sri Wiryawan I Kadek Yana Parastuta I Ketut Bawantika Adi Putra I Made Gotra I Made Muliarta I MADE MULIARTA . I Wayan Juli Sumadi I Wayan Sugiritama Ida Ayu Jasminarti Dwi Kusumawardani Ida Ayu, Priandini Iin Indrayani Maker, Luh Putu Ivana Juliarty Sitanggang Kadek Dwi Pradnyawati Kandy Rosa Ismalia Katrin Rotua Simbolon Ketut Mulyadi Komang Chandra Surya Dika Limantoro, Joshua Lisanthoni, Johnathan Luh Ayu Widayanti Luh Ayu Widayanti Luh Komang Ayu Widhiaty Karang Luh Putu Iin Indrayani Maker Luh Yeni Laksmini M. Arif Perdana Ariyansyah Made Agus Cahya Nugraha Koriawan Maker, Luh Putu Iin Indrayani Moestikaningsih . Nariswari, Ida Ayu Ista Nathaniela, Jocelin Ni Gusti Ayu Agung Manik Yuniawaty Wetan Ni Kadek Ayu Maya Damayanti Ni Luh Gede Yoni Komalasari, Ni Luh Gede Yoni Ni Made Adi Tarini Ni Made Mahastuti Ni Putu Ekawati Ni Wayan Candrawati Ni Wayan Winarti Nornazirah Binti Azizan Popi Imelda Margareth Sitompul Pramitasuri, Tjokorda Istri Putra, Putu Krishna B. S. Putri, Kadek Sri Adi Putu Ayu Widya Pramesti Putu Erika Paskarani Putu Febry Krisna Pertiwi Putu Julia Chandra Devi Raka-Sudewi A. A. Sang Ayu Putu Yuliantini Setiawan, I Gede Budhi Silvia Nuhyil Indriani Sinaga, Naomi Juliana Sri Mahendra Dewi, I Gusti Ayu Suastini, Kadek Denik Sumadi, I Wayan J. Susilawathi, Ni Made tanaka, Tanaka Tjandra Kristiana Volman Tampubolon Wetan, Ni Gusti Ayu Agung Manik Yuniawaty Wilananda, Ida Bagus Ardya Kurnia Wimpie I Pangkahila Winari, Ni Wayan