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All Journal International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Veteriner Jurnal Biologi Udayana Bumi Lestari Ecotrophic, Journal of Environmental Science SIMBIOSIS Jurnal Riau Biologia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Riset Biologi dan Aplikasinya Indonesian Biodiversity Journal Indonesian Biodiversity Journal
Ni Wayan Sudatri
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Veterinary

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Kualitas Spermatozoa Mencit yang Terpapar Radiasi Sinar-X Secara Berulang (SPERMATOZOA QUALITY OF MICE EXPOSED TO X-RAYS RADIATION IN REPEATED) Ni Wayan Sudatri; Ni Made Suartini; Anak Agung Sagung Alit Sukmaningsih; Dwi Ariani Yulihastuti
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In radiology, X-ray has been used to diagnose disease and therapy. However, behind the technologybenefits provided by the radiation, the negative effects are often debated. The purpose of this study was toinvestigate the effects of repeated radiation on sperm quality mice (Mus musculus L). Thirty- two adultmale mice aged three months were divided into groups P1 (1x 200 rad), P2 (2x200 rad), P3 (3x200 rad) andcontrol irradiated with x-rays according to the experimental design . Spermatozoa quality parametersobserved were : number of spermatozoa, motility, viability and morphology of spermatozoa. The results ofthe Post Hoc LSD tests for significant differences (P>0.05) between the control and treatment showed thatthe X-ray radiation exposure to 1x200 rad, 2x200 rad, and 3x200 rad decreases the motility, viability,normal morphology and number spermatozoa produced compared with controls. This is caused by exposureto X-ray radiation causes the formation of free radicals in the body that damage sperm cells mice. Exposureto X-ray radiation repeatedly lowered the quality of spermatozoa of mice.
PENGARUH PEMBERIAN RHODAMIN B TERHADAP SIKLUS ESTRUS MENCIT (Mus musculus L.) BETINA G.A.A Rina Febrina; Ngurah Intan Wiratmini; Ni Wayan Sudatri
Jurnal Biologi Udayana Vol 17 No 1 (2013): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

The research was conducted to identify the effect of rhodamine B on estrous cycle on female mice (Mus musculus L.). Twenty-four female mice were used, and randomly divided into four groups.  Rhodamine B was given orally (gavage) with a dose of  0 ppm (control), 150 ppm, 300 ppm, and 600 ppm in two estrous cycles.  Treatments were started when mice in estrus phases. The time between first and the next estrus phase were observed from vaginal smear preparation. Data obtained were analysed with One Way Anova.  If the differences were appeared between treatments, then the LSD test was performed. The results showed that rhodamine B was significantly increased the time of estrous cycle (P<0.05).
GANGGUAN SPERMATOGENESIS SETELAH PEMBERIAN MONOSODIUM GLUTAMAT PADA MENCIT (MUS MUSCULUS L.) A.A.SG.A SUKMANINGSIH; I GUSTI AYU MANIK ERMAYANTI; NGURAH INTAN WIRATMINI; NI WAYAN SUDATRI
Jurnal Biologi Udayana Vol 15 No 2 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

This study was conducted to investigate the effect of monosodium glutamate (MSG) on spermatogenesis of male experimental mice.using 32 twelve-week old mice. They were divided randomly into 4 groups, each group consisted of 8 mice. One group was used as control, three groups were used as treatment, which received 1.5 mg/g body weight/day, 3 mg/g body weight/day and 4.5 mg/g body weight/day of MSG solution in destilled water for 35 days. The result showed that MSG treatment led to disturbance on spermatogenesis by reduction in some of the parameter studied. There was a significant (p < 0.05) decrease in the number of pachytene spermatocyte and spermatid 15, where as the decrease in spermatogonia was insignificant (p>0.05).
Histomorfometrik duodenum kelinci jantan setelah diberi pakan komersial disuplementasi minyak hati ikan kod Ni Gusti Ayu Manik Ermayanti; Ni Wayan Sudatri; Ni Nyoman Wirasiti; I Gusti Ayu Manik Widhyastini
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p10

Abstract

Fish oil is a source of long-chain polyunsaturated fatty acids (LCPUFA) that can be used as supplementary feed in commercial feed to increase male rabbit productivity. PUFAs function in various physiological processes, including in the digestive system.This study aimed to determine the histomorphometrics of male rabbits duodenum after fed commercial feed supplemented by cod liver oil. The experimental design used was a Completely Randomized Design (CRD) with four feed packages, namely commercial feed without cod liver oil (P0) as a control, commercial feed supplemented with cod liver oil 3% (P1), 4.5% (P2) and 6% (P3). Each consists of five male local rabbits as replicates and given starting female rabbits 1-3 months. The parameters observed were villi height, villi width, crypt depth, and tunica mucosa thickness of the duodenum in male rabbits. The data obtained were analyzed with Anova and if significantly different were further tested with DMRT. The results showed that cod liver oil supplementation in commercial feed was significantly different (p<0.05) to the histomorphometric parameters (villi height, villi width, crypt depth, and thickness of the tunica mucosa) of the duodenum in male rabbits. Can be concluted that supplementation of cod liver oil in commercial feed can increase the histomorphometrics of male local rabbits duodenum.
KADAR KOLAGEN KULIT DAN TULANG PADA TIKUS BETINA USIA ENAM DAN 12 BULAN YANG DISUPLEMENTASI DENGAN SOMATOTROPIN Ni Wayan Sudatri
Jurnal Biologi Udayana Vol 14 No 1 (2010): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

The objective of this study is to investigate the effects of somatotropin supplementation on skin and bone collagen concentration of six-month and one-year old female mice. Forty eight female rats were assigned into a randomized block design with 4 factors. The first factor was age with 2 levels (6 months and 12 months). The second factor was somatotropin dosage with 2 levels (0 and 9 mg/kg body weight). The third factor was duration of injection with 2 levels (3 weeks and 6 weeks), and the fourth factor was sampling period with 2 levels (0 and 14 days after somatotropin termination). The results showed that somatotropin supplementation had significant effects on bone (P
JENIS-JENIS SERANGGA DI SEKITAR PANTAI PERERENAN, MENGWI, KABUPATEN BADUNG Ni G. A. Manik Ermayanti; Ni W. Sudatri; A. A. G. Raka Dalem
Bumi Lestari Journal of Environment Vol 7 No 2
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A Study on insects was carried out on Pererenan, District of Mengwi, Badung Regency, Baliprovince, between September and December, 2006. Samples were captured using insect nets. Thesamples were then identified for determination of their species. Their roles on the ecosystem weredescribed. Seven species of butterflies and 29 kinds of other insects were identified on this study.From those, none to be classified as protected species.
IDENTIFIKASI MAKROZOOBENTHOS DI TUKAD BAUSAN, DESA PERERENAN, KABUPATEN BADUNG, BALI Ni Made Suartin; Ni Wayan Sudatri; Made Pharmawati; A. A. G. Raka Dalem
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 5 No 1
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

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Abstract

A macrozoobenthos study was conducted at Bausan River, Pererenan village, Badung regency, Bali between September and December, 2006. There were six sampling stations were determined purposively. At each station, five unit of square plots of 40 cm x 40 (in maximum solum depth) samples were taken. The result showed that there were seventeen species of macrozoobenthos were found. There was no protected species found in this study. The macrozoobenthos had 2,28 level of index diversity. This indicated that the ecosystem at this area was in a stable condition with a medium level of macrozoobenthos diversity.
MORFOMETRI DAN KARAKTERISTIK FISIK TUMISI (Amphidromus peversus) DARI KAMPUS UNIVERSITAS UDAYANA BUKIT JIMBARAN, BALI-INDONESIA N. W. Sudatri; N. M. Suartini; A. A. G. Raka Dalem
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 2 No 2
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

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Abstract

A study on mollusks (Amphidromus peversus) has been carried out on Udayana University, BukitJimbaran (Bali, Indonesia) campus between November, 2003- November, 2005. The study was undertakenon a secondary forest on a limestone dry soil with dominant vegetation of Glerecydium sepium, Lantanacamara, Ceiba pentranda, and Acasia auriculiformis. Samples were randomly taken on quadrate samplings.Mollusks were captured on the land surface or on living vegetations. Results of the study showed that A.peversus has an average of shell length of 27.9 mm, shell width 18.0 mm , and body weight 7.90 g, number ofshell whorls 5.3, with the proportion of direction of whorl to the right of 55.3%. The shell of A. peversus hasno operculum. The color of the shell was yellow with brown stripes.The relationship between shell length (X) and with body weight (Y) can be represented by theequation of Y = 0.473 X – 5.32 (R2 = 11.9 %), which showed week relationships between those parameters.Meanwhile the relationships between the shell length (X1), width (X2) and body weight (Y) was best fitted bythe following equation : Y = 0.289 X1 + 0.0140 X2 – 3.47 (R2 = 94.9%).
DAYA HAMBAT INFUSA RIMPANG KUNYIT (Curcuma longa Linn) TERHADAP PERTUMBUHAN Escherichia coli dan Vibrio sp. pada IKAN KERAPU LUMPUR (Epinephelus tauvina) di PASAR KEDONGANAN KABUPATEN BADUNG, BALI Ni Putu Sinta Puspa Dewi; I. B. Gede Darmayasa; Ni Wayan Sudatri
SIMBIOSIS Vol 5 No 2 (2017)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2028.026 KB) | DOI: 10.24843/JSIMBIOSIS.2017.v05.i02.p04

Abstract

ABSTRACTFish is perishable food because it contains high enough protein and water, therefore to easy damaged by microorganism. Then the control of mircoorganism should be done to maintaining the quality of fish to stay awake.Turmeric is one of the herbs that has compound content curcumin and fenolik being able to control the growth of contaminants microorganism. The objective of this research is to find out the power of immersion of mudfish grouper (Epinephelus tauvina) by infusa turmeric rhizome (Curcuma longaLinn) through sampled from Kedonganan Market, Bali. The treatment given to mudfish grouper was turmeric rhizome infusion withconcentration of 0%, 5%, 10%, 15% and 20% tested in vitro and in vivo on the growth of E. coli and Vibrio sp. The resourcesinhibited turmeric infusa rhizome is determined by counting the population of bacteria test after treatment by the method of dilution sampling (Plating Method). The results showed that turmeric rhizome infusion was able significantly (P<0,05) inhibitionto the growth of E. coli and Vibrio sp. both in vitro and in vivo. The control (0%) in vitro population E. coli and Vibrio sp. each of 5,23x102 CFU/g and 4,98x102 CFU/g higher than with the treatment of concentration 5%, 10%, 15% and 20%. Population E. coli and Vibrio sp. in testing by in vivo (concentration 0%) each is obtained 4,17x102 CFU/g dan 4,20x102 CFU/g in statistic is different (P<0,05) with the concentration 10%, 15% and 20%. Keywords: Epinephelus tauvina, Curcuma longa Linn, E. coli, Vibrio sp.
EFEKTIVITAS EKSTRAK DAUN SUKUN (Artocarpus communis Forst.) DALAM MENURUNKAN KADAR GLUKOSA DARAH DAN MEMPERTAHANKAN JUMLAH SPERMA PADA TIKUS (Rattus norvegicus L.) I Putu Ari Wijana Dipa; Ni Wayan Sudatri; Ngurah Intan Wiratmini
SIMBIOSIS Vol 3 No 2 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

Breadfruit (Artocarpus communis Forst.) leaves have a high antioxidant content. This research aims to know the effect of breadfruit leaf extracts on blood sugar levels and the amount of sperm in rat. This study used a Completely Randomized Design (CRD) using 30 rats aged 2-3 months and weighing 150-200 grams. Male rats were divided into six groups, i.e. negative control group (K1) without treatment, groups K2, P1, P2, P3 and P4 are given then NaCl 0.9%, breadfruit leaf extracts 50 mg/kg, 100 mg/kg, 200 mg/kg body weight, and glibenclamide 1 mg/kg body weight respectively. The treatments were given every day for 21 days. Blood sugar levels were measured before and after aloksan administration, day 7, 14, and 21 after treatment as well as counting the number of sperm after 21 days. The data obtained were analyzed with ANOVA and Duncan Multiple Range Test (DMRT), using the program of SPSS For Windows version 22. Results of the study showed that breadfruit (Artocarpus communis Forst.) leaf extracts could lowered blood glucose levels and the most effective concentration was 100 mg/kg body weight of breadfruit leaf extracts given for 21 days with the effectiveness of 66,77%. Breadfruit leaf extracts did not inhibit spermatogenesis. So the amount of rat sperm did not different significantly among treatment. Keywords: blood sugar levels, breadfruit, rat, sperm count
Co-Authors A.A.S. A. Sukmaningsih Anak Agung Gde Raka Dalem Anak Agung Ketut Darmadi Andayani, Ni Kadek Prederika Sari Dwi Ariani Yulihastuti G.A.A Rina Febrina I G.A.P. Adnyana I G.S. Astawa I Gede Agus Pradana Putra I GUSTI AYU MANIK ERMAYANTI I Gusti Ayu Manik Widhyastini I Ketut Ginantra I KETUT MUKSIN I M. Widiartha I Putu Ari Wijana Dipa I Wayan Andi Yoga Kurniawan I Wayan Wena Diartha I. Harini I.G.A.S. Wahyuni Ida Bagus Gede Darmayasa Ida Bagus Gede Darmayasa Inabuy, Fainmarinat selviani IRIANI SETYAWATI Kadek Indah Kartika Sari Kadek Mardika Komang Nikastri Tussning Dewi LUH Puru ESWARYANTI KUSUMA YUNI MADE PHARMAWATI Ngurah Intan Wiratmini Ni G. A. Manik Ermayanti Ni LUH ARPIWI Ni Luh Watiniasih Ni Made Suartin Ni Made Suartini Ni Nyoman Wirasiti Ni Putu Adriani Astiti Ni Putu Sinta Puspa Dewi NI PUTU SRI PUSPITA WIDI YANTHI Putu Ayudina Asti Puspita Rai Suarni, Ni Made Ratnasari, Athalia Retno Kawuri Salsabillah Rahma SANG KETUT SUDIRGA Warmadewi, I Gusti Ayu Intan Yusni Khusnita Pangesti