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All Journal International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Veteriner Jurnal Biologi Udayana Bumi Lestari Ecotrophic, Journal of Environmental Science Jurnal Riau Biologia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Riset Biologi dan Aplikasinya Indonesian Biodiversity Journal Indonesian Biodiversity Journal SIMBIOSIS
Ni Wayan Sudatri
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Veterinary

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Kualitas Spermatozoa Mencit yang Terpapar Radiasi Sinar-X Secara Berulang (SPERMATOZOA QUALITY OF MICE EXPOSED TO X-RAYS RADIATION IN REPEATED) Ni Wayan Sudatri; Ni Made Suartini; Anak Agung Sagung Alit Sukmaningsih; Dwi Ariani Yulihastuti
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In radiology, X-ray has been used to diagnose disease and therapy. However, behind the technologybenefits provided by the radiation, the negative effects are often debated. The purpose of this study was toinvestigate the effects of repeated radiation on sperm quality mice (Mus musculus L). Thirty- two adultmale mice aged three months were divided into groups P1 (1x 200 rad), P2 (2x200 rad), P3 (3x200 rad) andcontrol irradiated with x-rays according to the experimental design . Spermatozoa quality parametersobserved were : number of spermatozoa, motility, viability and morphology of spermatozoa. The results ofthe Post Hoc LSD tests for significant differences (P>0.05) between the control and treatment showed thatthe X-ray radiation exposure to 1x200 rad, 2x200 rad, and 3x200 rad decreases the motility, viability,normal morphology and number spermatozoa produced compared with controls. This is caused by exposureto X-ray radiation causes the formation of free radicals in the body that damage sperm cells mice. Exposureto X-ray radiation repeatedly lowered the quality of spermatozoa of mice.
PENGARUH PEMBERIAN RHODAMIN B TERHADAP SIKLUS ESTRUS MENCIT (Mus musculus L.) BETINA G.A.A Rina Febrina; Ngurah Intan Wiratmini; Ni Wayan Sudatri
Jurnal Biologi Udayana Vol 17 No 1 (2013): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

The research was conducted to identify the effect of rhodamine B on estrous cycle on female mice (Mus musculus L.). Twenty-four female mice were used, and randomly divided into four groups.  Rhodamine B was given orally (gavage) with a dose of  0 ppm (control), 150 ppm, 300 ppm, and 600 ppm in two estrous cycles.  Treatments were started when mice in estrus phases. The time between first and the next estrus phase were observed from vaginal smear preparation. Data obtained were analysed with One Way Anova.  If the differences were appeared between treatments, then the LSD test was performed. The results showed that rhodamine B was significantly increased the time of estrous cycle (P<0.05).
GANGGUAN SPERMATOGENESIS SETELAH PEMBERIAN MONOSODIUM GLUTAMAT PADA MENCIT (MUS MUSCULUS L.) A.A.SG.A SUKMANINGSIH; I GUSTI AYU MANIK ERMAYANTI; NGURAH INTAN WIRATMINI; NI WAYAN SUDATRI
Jurnal Biologi Udayana Vol 15 No 2 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

This study was conducted to investigate the effect of monosodium glutamate (MSG) on spermatogenesis of male experimental mice.using 32 twelve-week old mice. They were divided randomly into 4 groups, each group consisted of 8 mice. One group was used as control, three groups were used as treatment, which received 1.5 mg/g body weight/day, 3 mg/g body weight/day and 4.5 mg/g body weight/day of MSG solution in destilled water for 35 days. The result showed that MSG treatment led to disturbance on spermatogenesis by reduction in some of the parameter studied. There was a significant (p < 0.05) decrease in the number of pachytene spermatocyte and spermatid 15, where as the decrease in spermatogonia was insignificant (p>0.05).
Histomorfometrik duodenum kelinci jantan setelah diberi pakan komersial disuplementasi minyak hati ikan kod Ni Gusti Ayu Manik Ermayanti; Ni Wayan Sudatri; Ni Nyoman Wirasiti; I Gusti Ayu Manik Widhyastini
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p10

Abstract

Fish oil is a source of long-chain polyunsaturated fatty acids (LCPUFA) that can be used as supplementary feed in commercial feed to increase male rabbit productivity. PUFAs function in various physiological processes, including in the digestive system.This study aimed to determine the histomorphometrics of male rabbits duodenum after fed commercial feed supplemented by cod liver oil. The experimental design used was a Completely Randomized Design (CRD) with four feed packages, namely commercial feed without cod liver oil (P0) as a control, commercial feed supplemented with cod liver oil 3% (P1), 4.5% (P2) and 6% (P3). Each consists of five male local rabbits as replicates and given starting female rabbits 1-3 months. The parameters observed were villi height, villi width, crypt depth, and tunica mucosa thickness of the duodenum in male rabbits. The data obtained were analyzed with Anova and if significantly different were further tested with DMRT. The results showed that cod liver oil supplementation in commercial feed was significantly different (p<0.05) to the histomorphometric parameters (villi height, villi width, crypt depth, and thickness of the tunica mucosa) of the duodenum in male rabbits. Can be concluted that supplementation of cod liver oil in commercial feed can increase the histomorphometrics of male local rabbits duodenum.
KADAR KOLAGEN KULIT DAN TULANG PADA TIKUS BETINA USIA ENAM DAN 12 BULAN YANG DISUPLEMENTASI DENGAN SOMATOTROPIN Ni Wayan Sudatri
Jurnal Biologi Udayana Vol 14 No 1 (2010): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

The objective of this study is to investigate the effects of somatotropin supplementation on skin and bone collagen concentration of six-month and one-year old female mice. Forty eight female rats were assigned into a randomized block design with 4 factors. The first factor was age with 2 levels (6 months and 12 months). The second factor was somatotropin dosage with 2 levels (0 and 9 mg/kg body weight). The third factor was duration of injection with 2 levels (3 weeks and 6 weeks), and the fourth factor was sampling period with 2 levels (0 and 14 days after somatotropin termination). The results showed that somatotropin supplementation had significant effects on bone (P
JENIS-JENIS SERANGGA DI SEKITAR PANTAI PERERENAN, MENGWI, KABUPATEN BADUNG Ni G. A. Manik Ermayanti; Ni W. Sudatri; A. A. G. Raka Dalem
Bumi Lestari Journal of Environment Vol 7 No 2
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A Study on insects was carried out on Pererenan, District of Mengwi, Badung Regency, Baliprovince, between September and December, 2006. Samples were captured using insect nets. Thesamples were then identified for determination of their species. Their roles on the ecosystem weredescribed. Seven species of butterflies and 29 kinds of other insects were identified on this study.From those, none to be classified as protected species.
IDENTIFIKASI MAKROZOOBENTHOS DI TUKAD BAUSAN, DESA PERERENAN, KABUPATEN BADUNG, BALI Ni Made Suartin; Ni Wayan Sudatri; Made Pharmawati; A. A. G. Raka Dalem
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 5 No 1
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

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Abstract

A macrozoobenthos study was conducted at Bausan River, Pererenan village, Badung regency, Bali between September and December, 2006. There were six sampling stations were determined purposively. At each station, five unit of square plots of 40 cm x 40 (in maximum solum depth) samples were taken. The result showed that there were seventeen species of macrozoobenthos were found. There was no protected species found in this study. The macrozoobenthos had 2,28 level of index diversity. This indicated that the ecosystem at this area was in a stable condition with a medium level of macrozoobenthos diversity.
MORFOMETRI DAN KARAKTERISTIK FISIK TUMISI (Amphidromus peversus) DARI KAMPUS UNIVERSITAS UDAYANA BUKIT JIMBARAN, BALI-INDONESIA N. W. Sudatri; N. M. Suartini; A. A. G. Raka Dalem
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 2 No 2
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

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Abstract

A study on mollusks (Amphidromus peversus) has been carried out on Udayana University, BukitJimbaran (Bali, Indonesia) campus between November, 2003- November, 2005. The study was undertakenon a secondary forest on a limestone dry soil with dominant vegetation of Glerecydium sepium, Lantanacamara, Ceiba pentranda, and Acasia auriculiformis. Samples were randomly taken on quadrate samplings.Mollusks were captured on the land surface or on living vegetations. Results of the study showed that A.peversus has an average of shell length of 27.9 mm, shell width 18.0 mm , and body weight 7.90 g, number ofshell whorls 5.3, with the proportion of direction of whorl to the right of 55.3%. The shell of A. peversus hasno operculum. The color of the shell was yellow with brown stripes.The relationship between shell length (X) and with body weight (Y) can be represented by theequation of Y = 0.473 X – 5.32 (R2 = 11.9 %), which showed week relationships between those parameters.Meanwhile the relationships between the shell length (X1), width (X2) and body weight (Y) was best fitted bythe following equation : Y = 0.289 X1 + 0.0140 X2 – 3.47 (R2 = 94.9%).
Pemberian Ekstrak Etanol Pelepah Batang Pisang Ketip Dapat Meringankan Polycystic Ovary Syndrome pada Tikus Putih Model Kadek Mardika; Ni Wayan Sudatri; Sang Ketut Sudirga
Jurnal Veteriner Vol 23 No 2 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.217 KB) | DOI: 10.19087/jveteriner.2022.23.2.186

Abstract

Polycystic ovary syndrome is a condition that affects the fertility of female reproductive organs. ketip banana pseudostem extract contains flavonoids, saponins, alkaloids, and vitamin C. It is known that ketip banana pseudostem extract can trigger cell proliferation and contain phytoestrogen compound. The purpose of this study was to determine the ability of ketip banana pseudostem extract on length of estrus cycle, histology structure and wight of reproductive organ, and estrogen hormone levels in white rats of PCOS model. White rats female Wistar strain aged 54 days used a total of 25 animals in this study. The treatment was given for 21 days by 1 ml/tail/day with the gavage method. Vaginal smear was began on 5th day of treatment every 12 hours/day for three estrus cycles (15 days). At the end of the study (21th day), the rats were dissected to take their ovaries and blood. The ovaries were weighed and then histological preparation with paraffin method and hematoxylin-Eosin staining. The parameters observed were length of estrus cycle, weight of ovary, number of ovary follicles, as well as estrogen hormone levels. The result of this study showed that ketip banana pseudostem extract can extend proestrus phases and estrus phases, increase estrogen hormone levels, increase corpus luteum number as significantly (p<0.05) on 150 mg/kgBW dosage.
Daya proteksi minyak atsiri bunga kenanga (Cananga odorata ) dalam sediaan lotion antinyamuk terhadap Aedes aegypti Putu Ayudina Asti Puspita; Ni Luh Arpiwi; Ni Wayan Sudatri
Jurnal Biologi Udayana Vol 26 No 2 (2022): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2022.v26.i02.p12

Abstract

Berbagai alternatif telah dilakukan untuk menghambat populasi nyamuk Aedes aegypti penyebab penyakit demam berdarah, diantaranya penggunaan lotion antinyamuk yang mengandung DEET. Untuk mengurangi resiko dan dampak negatif dari lotion antinyamuk yang mengandung DEET, maka salah satu upaya dilakukan pemanfaatan minyak atsiri kenanga (Cananga odorata) sebagai repellent atau antinyamuk alami. Penelitian ini bertujuan untuk mengetahui rendemen minyak atsiri bunga kenanga, untuk menganalisis konsentrasi minyak atsiri pada sediaan lotion yang memberikan daya proteksi tertinggi terhadap gigitan nyamuk Ae. aegypti, menganalisis tingkat kesukaan probandus terhadap formulasi lotion, dan untuk mengetahui ada tidaknya sensitivitas setelah memakai lotion yang mengandung minyak atsiri bunga kenanga. Minyak atsiri kenanga pada penelitian ini diperoleh dengan cara destilasi uap yang selanjutnya digunakan sebagai agen antinyamuk dalam sediaan lotion. Rata-rata rendemen minyak atsiri bunga kenanga dengan tiga kali ulangan diikuti dengan standar deviasi (SD) adalah 0,63% ± 1,38 b/b. Daya proteksi lotion antinyamuk tertinggi adalah lotion F3 dengan konsentrasi minyak atsiri 5%. Lotion F3 pada jam pertama memberikan daya proteksi sebesar 96,3%, jam kedua 92,3%, jam ketiga 87% dan menurun sampai jam keenam menjadi 75%. Formulasi yang paling disukai oleh probandus adalah lotion dengan kandungan minyak atsiri sebanyak 5% b/b. Setelah pemakaian lotion antinyamuk tidak ada kesan lengket dikulit dan tidak adanya gejala sensitivitas.
Co-Authors A.A.S. A. Sukmaningsih Anak Agung Gde Raka Dalem Anak Agung Ketut Darmadi Andayani, Ni Kadek Prederika Sari Deny Suhernawan Yusup Dwi Ariani Yulihastuti G.A.A Rina Febrina I G.A.P. Adnyana I G.S. Astawa I Gede Agus Pradana Putra I GUSTI AYU MANIK ERMAYANTI I Gusti Ayu Manik Widhyastini I Ketut Ginantra I KETUT MUKSIN I M. Widiartha I Putu Ari Wijana Dipa I Wayan Andi Yoga Kurniawan I Wayan Wena Diartha I. Harini I.G.A.S. Wahyuni Ida Bagus Gede Darmayasa Ida Bagus Gede Darmayasa Inabuy, Fainmarinat selviani IRIANI SETYAWATI Kadek Indah Kartika Sari Kadek Mardika Komang Nikastri Tussning Dewi LUH Puru ESWARYANTI KUSUMA YUNI MADE PHARMAWATI Mohamad Mediko Ngurah Intan Wiratmini Ni G. A. Manik Ermayanti Ni LUH ARPIWI Ni Luh Watiniasih Ni Made Suartin Ni Made Suartini Ni Nyoman Wirasiti Ni Putu Adriani Astiti Ni Putu Sinta Puspa Dewi NI PUTU SRI PUSPITA WIDI YANTHI Putu Ayudina Asti Puspita Rai Suarni, Ni Made Ratnasari, Athalia Retno Kawuri Salsabillah Rahma SANG KETUT SUDIRGA Warmadewi, I Gusti Ayu Intan Yusni Khusnita Pangesti