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In vitro antifungal activity of trembesi leaf extract [Samanea saman (Jacq.) Merr.] against Colletotrichum magnum Rossman & Allen, the causal agent of papaya anthracnose Ratnasari, Athalia; Darmadi, Anak Agung Ketut; Inabuy, Fainmarinat selviani; Sudatri, Ni Wayan
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 2 (2025): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.225317-327

Abstract

Papaya fruit production in Bali has decreased over the last three years (2020-2022). One of the contributing factors is infection by Colletotrichum spp., which causes anthracnose. Anthracnose is an airborne disease that infects various plant commodities including papaya. The use of chemical fungicides to control anthracnose can have negative impacts if used long-term. Trembesi [Samanea saman (Jacq.) Merr.] extract has previously been reported to possess antifungal, antiseptic, antibacterial, and antidiabetic properties. This study aimed to determine the effectiveness and category of inhibitory activity, Minimum Inhibitory Concentration (MIC), Lethal Concentration 50% (LC??), and the phytochemical content of trembesi leaves. Methanol extract of trembesi leaves effectively inhibited the growth of Colletotrichum magnum at a concentration of 5%. The experiment used 11 treatments with 4 replications, including control (0% v/v) and extract concentrations of 1% to 10% (v/v), along with a positive control. The corresponding inhibition zone diameters were: 0.00 mm, 12.00 mm, 11.87 mm, 13.62 mm, 16.00 mm, 19.62 mm, 15.75 mm, 16.87 mm, 17.87 mm, 17.87 mm, 18.25 mm, and 20.87 mm, respectively. The minimum concentration of extract showing inhibitory activity (MIC) was 0.3%, while the LC?? value was 0.32%. Phytochemical screening revealed the presence of phenolics, tannins, alkaloids, steroids, terpenoids, flavonoids, and saponins in the trembesi leaf extract.
PENGARUH PEMBERIAN VARIASI PAKAN FERMENTASI TERHADAP PERTUMBUHAN DAN KEPADATAN ROTIFERA (Brachionus plicatilis ) UNTUK PAKAN NENER IKAN BANDENG (Chanos chanos) Mohamad Mediko; Ni Wayan Sudatri; Deny Suhernawan Yusup
SIMBIOSIS Simbiosis Volume 13 No. 1 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i09.

Abstract

Brachionus plicatilis is one of the zooplankton that is needed as food for fish larvae. So it is expected that the use of this alternative feed can help hatchery farmers to increase Rotifera production and increase the production of high quality nener. The objectives of the study were to determine the growth of Rotifera in cultures given variations of 4 additional feed ingredients fermented from chicken feces, wheat bran, rice bran and corn bran and to determine the growth response of milkfish (Chanos chanos) seeds fed with Rotifera from the best culture. The additional feed treatment given consisted of 5 factors (4 treatments and 1 control) fermented dried chicken feces, wheat bran, corn bran and rice bran, while the control was phytoplanton from UD Chlorella culture. The fermentation material used was EM4 (Efective Microorganisms 4 from Pt. Songgolangit Persada) The design used was a Randomized Group Design (RAK) with 4 replicate cultures. Giving variations of 4 feed ingredients that have been fermented affects the growth of Rotifera cultures. Growth can be seen from the density of each variation of 4 fermented ingredients namely wheat bran 276,750, corn bran 422,050, rice bran 567,650, chicken feces 169,450, Phytoplankton 77,300. Giving Rotifera cultured with rice bran to the growth of milkfish larvae influenced their growth. Milkfish larvae can grow after being given fermented Rotifera, but the SR value is very low. Milkfish larvae fed with fermented Rotifera had a low SR value of 32,73% mortality rate of 1,373 of 4,500 fish, compared to the SR value of Phytoplanton which was high at 78,15% mortality rate of 3,517 of 4,500 fish. Keywords: Cultivation, Bran, Feces, Culture, Rotifera