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All Journal Caraka Tani - Agriculture Science Journal Jurnal Hortikultura BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Caraka Tani: Journal of Sustainable Agriculture Biosaintifika: Journal of Biology & Biology Education Jurnal Rekayasa Lingkungan SINTA Journal (Science, Technology, and Agricultural) Jurnal Pengabdian Kolaborasi dan Inovasi IPTEKS Jurnal Pendidikan dan Pengabdian Masyarakat Jurnal Inspirasi Peternakan
Suliasih Suliasih
Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia, Jln. Raya Jakarta-Bogor Km. 46, Cibinong Science Center, Jawa Barat, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Physics Social Sciences Veterinary Other

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KEBERADAAN BAKTERI PELARUT FOSFAT DAN AKTIVITAS ENZIM FOSFATASE TANAH DAERAH PERAKARAN TANAMAN OBAT DARI KEBUN RAYA CIBODAS suliasih, Suliasih
Jurnal Rekayasa Lingkungan Vol 6, No 3: Jurnal Rekayasa Lingkungan
Publisher : BPPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.563 KB)

Abstract

A study was undertaken to investigate to occurance of phosphate solubilizing bacteria from rhizosphere soil samples of medicine plants in Cibodas Botanical Garden. 13 soil samples of medicine plants are collected randomly The result shows that 71 isolates of phosphate solubilizing bacteria were isolated, and 10 species of these organism was identified as Azotobacter sp, Bacillus sp, Chromobacterium sp, C.violaceum, Citrobacter sp. , Enterobacter sp., E. liquefaciens. Nitrosomonas sp., Serratia rubidaea, Sphaerotillus natans. Azotobacter sp. And Bacillus sp. Are found in all of soil tested. Conversely, Serratia rubidaea is only in the sample from rhizosphere of Plantago mayor The activity of acid alkaline phosphatase in soil tested ranged from 0.78 – 60,18 ugp nitrophenole/g/h, with the higest values being recorded in soil sample from rhizosphere of “Lavender”.Keywords : phosphate solubilizing bacteria, soil enzyme phosphatase
PENGUJIAN PUPUK HAYATI KALBAR UNTUK MENINGKATKAN PRODUKTIVITAS TANAMAN KEDELAI (Glycine max) var. BALURAN Subowo, Yohanes Bernard; Sugiharto, Arwan; Suliasih, Suliasih; Widawati, Sri
Caraka Tani: Journal of Sustainable Agriculture Vol 25, No 1 (2010): March
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.022 KB) | DOI: 10.20961/carakatani.v25i1.15756

Abstract

Research aimed to evaluate the ability of kalbar biofertilizer to improve productivity of soybean. Experiment was conducted in the field experimental plot of Cibinong Science Centre. Soybean (Glycine max) var Baluran was treated with a series of fertilizer i.e.: compost containing Nitrogen fixing microbes, compost containing Phosphate solubilizing microbes, compost containing lignocelullosic degrading fungus, Kalbar biofertilizer (compost containing Nitrogen fixing, Phosphate solubilizing and lignocellulosic degrading microbes), chemist fertilizer, compost and control. The height of plant, fresh weight of biomass, number of leaves, pod number, and weight of seed were observed. The Nitrogen, Phosphate and Carbon which exist on the soil observed before planting, while flowering and while harvesting. The result was the application of kalbar biofertilizer able to increase the Carbon (C), Nitrogen (N) and Phosphor (P) content in the soil. This treatment also increased the fresh weight of biomass (22%), the number of pods (11, 11%) and the weight of seed (12,22%).
PENGARUH INTRODUKSI KOMPOS PLUS TERHADAP PRODUKASI BOBOT KERING DAUN KUMIS KUCING (ORTHOSIPHON ARISTATUS) PADA TIGA MACAM MEDIA TANAH Widawati, Sri; Suliasih, Suliasih; Syaifudin, Syaifudin
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3475

Abstract

ABSTRACTA green house experiment were conducted to study the application of ?compost plus? on the growth of Orthosiphon aristatus. The experiment was designed in Complete Randomized Design with factorial and five replicates. The first factors were fertilizer application (compost plus, compost, and control). The second factors were 3 kinds of soil (i.e. soil from Cibinong, Ciomas, and Sukabumi). The result showed that compost plus application increased 113.90 gram/pot of dry weight of Orthosispon aristatus leaves compared with compost application and control in 3 kinds of soil.Key words : Compost plus (compost + microbes), Orthosispon aristatus
DAYA PACU MIKROBA PELARUT FOSFAT DAN PENAMBAT NITROGEN PADA TANAMAN JAGUNG Latupapua, H.J.D.; Suliasih, Suliasih
JURNAL BIOLOGI INDONESIA Vol 3, No 2 (2001): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i2.3477

Abstract

ABSTRACTThe Stimulating Capacities of the Phosphate Solubilizing and the Nitrogen Fixing Microbes on the Maize Plant. The green house experiment has been carried out to study the effectiveness of the phosphate solubilizing microbes (PSM) and nitrogen fixing bacteria (NFB) on the growth of maize (Zea mays). The phosphate solubilizing microbes were Aspergillus sp. and Gigaspora margarita while the nitrogen fixing bacteria were Azospirillum sp. and Azotobacter sp. have been used in this experiment. The aim of the experiment was to find the suitable and effective inoculant on the maize and its expected the growth of the plant. The experiment used The Completely Randomize Design with three replicates. The treatments were uninoculated plant (control), the plants inoculated with single microbe of PSM, NFB and the combination of both. The results indicated that the phosphate solubilizing microbes (PSM) and the nitrogen fixing bacteria (NFB) which were either used in single or combination formula could stimulate the plant growth. The dual inoculation of Azotobacter sp. and Aspergillus sp. were found the higest yield of maize.Key words : The phosphate solubilizing microbes, the nitrogen fixing bacteria, Mycorrhiza vesicular arbuscular, Zea mays.
KEBERADAAN BAKTERI PELARUT FOSFAT DAN AKTIVITAS ENZIM FOSFATASE TANAH DAERAH PERAKARAN TANAMAN OBAT DARI KEBUN RAYA CIBODAS suliasih, Suliasih
Jurnal Rekayasa Lingkungan Vol 6, No 3: Jurnal Rekayasa Lingkungan
Publisher : BPPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.563 KB)

Abstract

A study was undertaken to investigate to occurance of phosphate solubilizing bacteria from rhizosphere soil samples of medicine plants in Cibodas Botanical Garden. 13 soil samples of medicine plants are collected randomly The result shows that 71 isolates of phosphate solubilizing bacteria were isolated, and 10 species of these organism was identified as Azotobacter sp, Bacillus sp, Chromobacterium sp, C.violaceum, Citrobacter sp. , Enterobacter sp., E. liquefaciens. Nitrosomonas sp., Serratia rubidaea, Sphaerotillus natans. Azotobacter sp. And Bacillus sp. Are found in all of soil tested. Conversely, Serratia rubidaea is only in the sample from rhizosphere of Plantago mayor The activity of acid alkaline phosphatase in soil tested ranged from 0.78 ? 60,18 ugp nitrophenole/g/h, with the higest values being recorded in soil sample from rhizosphere of ?Lavender?.Keywords : phosphate solubilizing bacteria, soil enzyme phosphatase
Pengaruh Kompos yang Diperkaya Bakteri Penambat Nitrogen dan Pelarut Fosfat terhadap Pertumbuhan Tanaman Kapri dan Aktivitas Enzim Fosfatase dalam Tanah Widawati, S; Suliasih, Suliasih; Muharam, Agus
Jurnal Hortikultura Vol 20, No 3 (2010): September 2010
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v20n3.2010.p%p

Abstract

ABSTRAK. Penggunaan pupuk organik dalam budidaya sayuran memiliki beberapa keuntungan, terutama untukmempertahankan kondisi tanah dan menekan penggunaan pupuk anorganik. Penelitian dilaksanakan di DesaCidawu (1.250 m dpl.), Cibodas, Kabupaten Cianjur, Provinsi Jawa Barat, mulai bulan Januari sampai Desember2007. Sampel tanah dikoleksi dari daerah berbeda di Pontianak, Kalimantan Barat. Penelitian bertujuan mengetahuipengaruh kompos yang diperkaya dengan bakteri pelarut fosfat (phosphate solubilizing bacteria=PSB) terhadappertumbuhan tanaman kapri dan aktivitas enzim fosfatase di dalam tanah. Penelitian menggunakan rancangan acakkelompok dengan lima perlakuan dan tiga ulangan. Varietas kapri yang digunakan ialah varietas lokal. Perlakuanterdiri atas (A) tanpa pupuk, (B) pupuk anorganik (TSP+KCl+Urea), (C) kotoran ayam + sekam, (D) kompos, dan(E) kompos plus, yaitu kompos yang diberi campuran bakteri, yaitu bakteri penambat nitrogen simbiotik, bakteripenambat nitrogen nonsimbiotik, dan bakteri pelarut fosfat. Hasil penelitian menunjukkan bahwa campuran bakteriyang diisolasi dari tanah gambut di Kalimantan Barat yang terkandung dalam kompos plus dapat beradaptasi denganbaik pada lahan di lokasi penelitian. Populasi total SNFB, NSNFB, dan PSB meningkat masing-masing menjadi 9,15x 108, 9,34 x 108, dan 9,35 x 108 sel/g tanah. Keberadaan campuran bakteri tersebut mampu meningkatkan aktivitasenzim fosfatase asam dan basa di dalam tanah. Peningkatan tertinggi aktivitas enzim fosfomonoesterase asam danbasa dicapai pada perlakuan kompos plus. Perlakuan tersebut juga meningkatkan berat buah kapri dibandingkandengan kontrol (75,32 %), perlakuan pupuk kimia (45,48%), kotoran ayam + sekam ( 31,19 %), dan kompos (15,60%). Pemanfaatan campuran bakteri pelarut fosfat tersebut dalam kompos diharapkan dapat digunakan secara meluasdalam pembudidayaan kapri dalam sistem organic farming (OF), sehingga berperan dalam peningkatan produksi dansekaligus menekan penggunaan pupuk anorganik.ABSTRACT. Widawati, S., Suliasih, and A. Muharam. 2010. The Effect of Compost Enriched with SymbioticNitrogen Fixing and Phosphate Solubilizing Bacteria on the Growth of Peas and the Activity of PhosphataseEnzymes in the Soil. The use of organic materials on cultivation of vegetable crops has some advantages, especiallyfor maintaining suitable soil conditions and decreasing the utilization of inorganic fertilizers. The research wascarried out at Cidawu Village, Cibodas (1,250 m asl.), Cianjur, West Java, from January to December 2007. Soilsamples were collected from some different areas in Pontianak, West Kalimantan. The research was aimed todetermine the effect of compost enriched with mixed phosphate solubilizing bacteria (PSB) on the growth of peasand on the activity of phosphatase enzymes in the soil. A randomized block design with five treatments and threereplications was used in the experiment. A local variety of peas was utilized in the experiment. The treatments werethe addition of fertilizers consisted of (A) without any fertilizer, (B) with inorganic fertilizers i.e. TSP+KCl+Urea,(C) with chicken dung + rice husk, (D) with compost, and (E) with compost plus (compost enriched with symbioticnitrogen fixing bacteria/SNFB, nonsymbiotic nitrogen fixing bacteria/NSNFB, and PSB). The results showed thatthe mixed bacteria isolated from peat soil in West Kalimantan in the compost plus (treatment E) properly adaptedin soil conditions at the site of the experiment. The total populations of SNFB, NSNFB, and PSB were increased upto 9.15 x 108, 9.34 x 108, and 9.35 x 108 cell/g soil, respectively. The occurrence of the mixed bacteria increased theactivity of acid and alkaline phosphatases in the soil. The highest activities of acid and alkaline phosphomonoesteraseenzymes in the soil achieved by the treatment of compost plus. The treatment increased the fresh weight of peascompared to control (75.32 %), to the chemical fertilizers (45.48%), to chicken dung+rice husk ( 31.19 %), and tocompost (15.60 %). The widely application of the mixed PSB in compost is hopefully established in cultivation ofpeas in the organic farming (OF) system, then it will confidently support on the increase of peas production, and thedecrease of inorganic fertilizers as well.
The application of Rigidoporus sp J12 and Stenotrophomonas maltophilia BM in the degradation of batik waste Subowo, Yohanes; Suliasih, Suliasih; Widawati, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.41987

Abstract

The batik industry in Indonesia produces batik waste which pollutes the environment. This waste can be degraded using laccase-producing microorganisms. The microorganisms used in the research were the fungus Rigidoporus sp J12 and the bacteria Stenotrophomonas maltophilia BM. This research aims to determine the ability of Rigidoporus sp J12 and Stenotrophomonas maltophilia BM and their consortium in producing laccase, observing their ability to degrade Poly R-478 which is an indicator of phenoloxidase activity and batik waste. Microorganisms are grown in growth media and then placed in media containing Poly R-478 or batik waste. Inducers are added to increase laccase activity. The inducers used were 15 g/L sucrose, 200 µM CuSO4 and 40 mM veratryl alcohol. The results showed that Rigidoporus sp J12 and Stenotrophomonas maltophilia BM produced laccase in PDB and NA media. The highest laccase activity was found in the enzyme produced by Rigidoporus sp J12 in PDB media at a temperature of 40°C, media pH 6.0 and the addition of sucrose. Rigidoporus sp J12 degraded batik waste by 39.38% and increased by 2.12 times after adding sucrose and incubation for 15 days. These bacteria and fungi can be used to degrade batik waste in order to prevent environmental pollution. Using the fungus Rigidoporus sp J12 purely is more profitable than using it with S. maltophilia BM bacteria.
PENGARUH PENAMBAHAN BUAH MANGGA TERHADAP KADAR LEMAK, KADAR PROTEIN, DAN pH PADA YOGHURT DRINK MENGGUNAKAN STARTER KOMERSIL Erlando, Megi; Malianti, Lezita; suliasih, Suliasih
Jurnal Inspirasi Peternakan Vol. 3 No. 1 (2023): Jurnal Inspirasi Peternakan
Publisher : Fakultas Pertanian dan Peternakan Universitas Muhammadiyah Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36085/jinak.v3i1.5013

Abstract

Susu merupakan bahan yang mudah sekali rusak rentan dengan kontaminasi bakteri sehingga membutuhkan penanganan khusus untuk meningkatkan nilai manfaat dan daya simpan yang lebih lama, untuk itu peng. Yoghurt merupakan salah satu produk hasil dari fermentasi susu dengan penambahan kultur bakteri asam laktat yaitu Streptococcus thermophilus, dan Lactobacillus bulgaricus. Percobaan dilakukan dengan menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 4 ulangan . Sari buah mangga dibuat dengan penambahan A = 0%, B = 3%, C = 6% dan D =9%. Parameter yang diamati adalah kadar lemak, kadar protein, dan pH. Hasil penelitian memperlihatkan penambahan sari buah mangga berpengaruh nyata (p<0.05) terhadap kadar lemak, tingkat kadar protein dan pH. Hasil penelitian dapat disimpulkan bahwa semakin tinggi penggunaan sari buah mangga yang digunakan semakin menurunkan kadar lemak, ph dan meningkatkan kadar protein. Kata kunci : Susu, Yoghurt, Mangga, Streptococcus thermophilus, dan Lactobacillus bulgaricus.
PENGARUH PEMBERIAN TEPUNG UMBI GADUNG ( Dioscorea hispida Dennst ) DALAM RANSUM TERHADAP pH, NH3 DAN PRODUKSI VFA ( Volatille Fatty Acids ) SECARA IN- VITRO Harmawati, Lilis; Rita, Wismalinda; Sunaryadi; suliasih, Suliasih
Jurnal Inspirasi Peternakan Vol. 3 No. 3 (2023): Jurnal Inspirasi Peternakan
Publisher : Fakultas Pertanian dan Peternakan Universitas Muhammadiyah Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36085/jinak.v3i3.5849

Abstract

Tujuan penelitian ini adalah untuk mengetahui pengaruh penggunaan tepung umbi gadung padaransum terhadap produksi pH, NH3 dan VFA (volatile fatty acids ) pada ternak secara in vitro.Penelitian ini telah dilaksanakan pada 11 Mei sampai 7 Juli 2018 di Bukit Peninjauan 1, KecamatanSukaraja Kabupaten Seluma. Analisa kecernaan ransum akan di lakukan di laboraturium Ilmu danTeknologi Pakan serta Laboraturium Institusi Pertanian Bogor ( IPB). Penelitian ini menggunakanRancangan Acak Lengkap (RAL) dengan 4 perlakuan (0%, 10%, 20%, 30%) dan 3 kelompokulangan. Materi yang digunakan adalah ransum yang terdiri dari tepung umbi gadung, tepungjagung, tepung ikan rucah, dan dedak. Parameter yang diamati meliputi nilai pH, NH3 dan produksiVFA (Volatille Fatty Acids). Data dianalisis menggunakan analisis ragam (ANOVA), antara NH3dan VFA (Volatille Fatty Acids) berpengaruh sangat nyata, namun pH tidak berpengaruh nyata. Daripenelitian ini dapat disimpulkan bahwa pengaruh penambahan tepung umbi gadung pada ransumterhadap pH, NH3 dan VFA (Volatille Fatty Acids) tidak mengganggu aktifitas fermentasi mikrobarumen.Kata kunci: pH, NH3, VFA dan Tepung Umbi Gadung
Effect Of Egg Weight On Hatchability And Hatching Weight Of Quail (Cortunix cortunix Japonica) Zurina, Rita; Malianti, Lezita; Definiati, Neli; Rita, Wismalinda; Suliasih, Suliasih
SINTA Journal (Science, Technology, and Agricultural) Vol. 4 No. 2 (2023)
Publisher : Perkumpulan Dosen Muda (PDM) Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37638/sinta.4.2.209-214

Abstract

The research was conducted to determine the fertility, hatchability and hatching weight of quail (Corturnix-corturnix japonica) eggs in various egg weights. The research was carried out experimentally using a Randomized Block Design (RAK) consisting of 3 treatments and 4 replications. This research will be carried out from October to December 2020 at Kandang Puyuh, Kasih Ibu Orphanage, Jalan Bali, Bengkulu City. The tools used in this research were 4 manual hatching machines, box-shaped with a capacity of around 300 quail eggs, scales and an electrical energy source. The materials used are 300 quail eggs and disinfectant. The research was carried out experimentally using a Randomized Block Design (CRD) with 3 treatments and 4 replications. The treatment applied is egg weight consisting of: B1 = Light (≤9.5g), B2 = Medium (9.6 – 10.5g), B3 = Heavy (≥10.6g)
Co-Authors Afifah Nurul Putri Agus Muharam Arwan Sugiharto Atmaja, Loliek Kania Erlando, Megi Harmawati, Lilis Hartati Imamuddin Istikomah, Julia Jelita Zakaria LATUPAPUA, H.J.D. Latupapua, H.J.D. Malianti, Lezita Maman Rahmansyah Neli Definiati Novitasari Novitasari Rita, Wismalinda S Widawati Septiani, Anggri Sri Widawati Subowo, Yohanes Sunaryadi Syaifudin Syaifudin, Syaifudin YB. Subowo Yohanes Bernard Subowo, Yohanes Bernard Zurina Muchlis, Rita