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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Majalah Ilmiah Peternakan Jurnal Sain Peternakan Indonesia Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Tropical Animal Science Journal Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY
C. SUMANTRI
Departemen Ilmu Produksi dan Teknologi Peternakan, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Veterinary Other

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SINGLE NUCLEOTIDE POLYMORPHISMS OF LIPOPROTEIN LIPASE GENE AND ITS ASSOCIATION WITH MARBLING QUALITY IN LOCAL SHEEPS Hidayati, H.; Sumantri, C.; Noor, R. R.; Priyanto, R.; Rahayu, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 1 (2015): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.1.1-10

Abstract

Lipoprotein lipase (LPL) is a key enzyme that plays in metabolism and transport lipoprotein andtherefore has an influence on blood triglyceride levels. LPL controls triacylglycerol partitioning betweenadipose tissue and muscle that increases fat storage or provides energy in the form of fatty acids formuscle growth. The research was aimed to explore Single Nucleotide Polymorphisms of LPL gene andto associate SNP with marbling quality. A total of 66 genomic DNAs consisted of sumatera thin-tail edsheep (50 heads) and garut sheep (16 heads) were used in this study. Polymerase Chain Reaction wasused to amplify genomic DNA and direct sequencing method was to identify polymorphism sequences.The sequences were analyzed with Bio Edit and MEGA 5.2. The BLAST sequence was obtained fromgene bank X.68308.1. The association between the genotype and marbling quality was analyze by oneway ANOVA and further between mean differences were tested using least sgnificant difference. Theresults showed that 3 novel SNPs i.e. insertion g.26>C; insertion g.27> G and c.192T>C on garut sheepand a SNP insertion g.26>C/G on sumatera thin-tail ed sheep. The diversity of LPL gene at c.192T>Cwas associated with heneicosanoic acid, whereas TT genotype (0.04%) was higher than CC (0.03%) andCT (0.02%).
κ-CASEIN GENE POLYMORPHISMS IN RIVERINE AND SWAMP BUFFALO IN INDONESIA Rini, A.O.; Sumantri, C.; Damayanthi, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 1 (2014): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.1.1-9

Abstract

Kappa-casein (κ-casein) gene is known as a gene that plays a role in controlling milk protein andalso play a crucial role in the coagulation and curdling of milk. This study was aimed to identifypolymorphisms of the κ-Casein gene of local buffaloes in Indonesia. A total number of 40 heads ofriverine buffalo and 250 heads of swamp buffalo. This study used PCR-RFLP method, whichamplification of the κ-Casein gene resulted an amplicon with length of 157 bp, located in exon 4. Theamplified fragment were digested with EcoRV restriction enzyme, which cut the κ-Casein gene in exon4 at nucleotides of GAT|ATC, revealed the presence of one polymorphism at the base position of 23 bpthat occurs with a substitution of Ile (ATC) of the T genetic variant into Thr (ACC) of the C geneticvariant. Genotyping κ-Casein gene in riverine buffalo produced two types of allele, namely C allele (157bp) and T allele (136 and 21 bp). These two alleles resulted in three types of genotypes, namely CC, CT,and TT. Frequency of the C allele was dominant to T allele. κ-Casein gene in swamp buffalo wasmonomorphic with one allele, namely C allele. Heterozygosity value of riverine and swamp buffaloeswere low. PIC value in riverine and swamp buffalo ranged 0.000-0.288. Fixation index of κ-Casein genein riverine buffalo was low (Siborong-borong SBBC = -0,0036; Deli Serdang = -0.025), but in swampbuffalo was in fixation. This study showed that κ-Casein|EcoRV were polymorphic in riverine buffaloand monomorphic in swamp buffalo.
PRODUCTIVITY, REPEATABILITY OF PRODUCTIVE AND REPRODUCTIVE TRAITS OF LOCAL PIGEON Darwati, S.; Martojo, H.; Sumantri, C.; Sihombing, D.T.H.; Mardiastuti, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 4 (2010): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.4.268-274

Abstract

The purpose of this study was to know productivity and repeatability of local pigeon. Data werecollected from 124 birds that reared under intensive management. The results showed that artificial pairwas 100% and polygamy was 16% (n=62 pair of pigeon). The ration of local pigeon consisting of 50%corn+50% of commercial feed for starter broiler chicken can be applied in field. The average of eggproduction was 1.8 eggs/pair/period, egg weight was 17.7 g, fertility was 96.6%, hatching rate was 77%,embryo mortality rate was 23%, interval period from laying to hatching and suckling was 51 days, 31.4days with hatching, and 17.6 days without hatching and suckling. The day old pigeon weight ranged10.9-16.2 g. Repeatability value of productive traits was high, in which egg weight was 0.64 and day oldpigeon weight was 0.737. Repeatability of reproductive traits was low, that was fertility and hatchabilitywas 0.12 and 0.048, respectively). The squab weight increased from week 0 to 4, then decreased in theweek 5. The growth rate was highest at the week 1, then decreased from the week 2 to 5 with thenegative growth rate occur at the 5th week. The squab growth rate followed a quadratic pattern. It wasconcluded that slaughter squab selection could be done at 4th week old.
IDENTIFICATION OF POLYMORPHISM OF FSH BETA-SUBUNIT GENE AS SPERM QUALITY MARKER IN BALI CATTLE USING PCR-RFLP Ishak, A.B.L.; Sumantri, C.; Noor, R.R.; Arifiantini, I.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 4 (2011): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.4.221-227

Abstract

The aim of study was to identify the association of FSH beta-subunit gene polymorphisms withsperm quality traits. A total of 470 samples of normal mature bull from several breeds were used forpopulation study and 127 bulls from National and Regional AI centre of Indonesia for association study.To amplify, a PCR-RFLP method was used and digested with Pst1 restriction enzyme. The allelefrequency of the A and B in Bali cattle were (0.000) and (1.000), respectively. The absence of otherallele A suggested that the Bali cattle was monomorphic, while Brahman, FH, Simmental and Limousinewere polymorphic. The highest observed heterozygosity were found in Limousine (0.318) and thehighest expected heterozygosity were in Simmental (0.420). The higher incident of percentage of spermabnormalities were found in Simmental, Limousin, Brahman compared to Bali and FH. Among all typesof sperm abnormalities, the abaxial and microcephalus were found in highest number.
IDENTIFICATION OF MYOSTATIN GENE c.960delG LOCUS POLYMORPHISM IN INDONESIAN LOCAL SHEEP BY USING PCR-SSCP METHOD Sumantri, C.; Jakaria, J.; Yamin, M.; Nuraini, H.; Andreas, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 3 (2011): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.3.145-151

Abstract

Myostatin (MSTN) is a protein inhibit muscle growth. This protein is a member of a superfamily ofmolecules called transforming growth factors beta (TGF-b). Deletion in c.960delG (1-bp deletion atposition 960) disrupts the reading frame from amino acid (aa) position 320 to ending in a premature stopcodon in aa position 359 have been found in Norwegian White Sheep. This deletion in the myostatingene is responsible to increase muscle mass, also known as 'double muscling', in sheep. The purpose ofthis study was to identify the polymorphism of myostatin gene in c.960delG locus of local sheep inIndonesia. The 832 DNA sampels from sheep were collected from 13 populations belonging to thePriangan (86), Javanese Thin Tail (389 i.e. Jonggol, Banjar, Ciomas), Javanese Fat Tail (94), West NusaTenggara (136), Rote Island/East Nusa Tenggara (35), Kisar Island/Southwest Maluku (22),Donggala/South East Celebes (45) and Batur cross breed/Wonosobo, Central Java (25). A gene fragmentof MSTN c.960delG length 299 bp was successfully amplified by using the technique of PCR(polymerase chain reaction) and genotyped by SSCP (single strand conformation polymorphism). Theresult showed no polymorphism in this gene. All sheep tested had G/G genotype for c.960delG locus.
PRODUCTIVITY AND GENETIC DIVERSITY OF LOCAL CATTLE IN CIAMIS-WEST JAVA Hilmia, N.; Noor, R.R.; Sumantri, C.; Gurnadi, R.E.; Priyanto, R.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 1 (2013): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.1.10-19

Abstract

The objectives of this study were to identify the productivity and genetic diversity of local cattle inCiamis West Java based on DNA microsatellite, in order to provide the basic information for its rearing,conservation and development. Eighteen of local cattle were kept for 58 days by feeding concentratesand rice straw. The measured parameters were weekly body weight as well as carcass percentage. Thepercentage of comparing carcass was taken from PO, Bali and crossbred cattle from local cattle withLimousin, Simmental, Brahman and Angus cattle. Data were analyzed using Excel software and SAS(2004). Blood samples were taken from two subpopulations i.e., 46 and 52 samples from Tambaksariand Cijulang areas, respectively. Other DNA samples were collected from Bali, PO, Limousin, Simental,Brahman and Angus cattle. Genetic diversity and phylogenetic analysis were analyzed using 3microsatellite loci, that were INRA35, HEL9 and BM2113. The genotyping data were analyzed usingPOPGENE.32 program, while phylogenetic trees were analyzed by MEGA 4. Average daily gain (ADG)of local cattle in Ciamis was 0.62±0.23 kg/head/day with a carcass percentage was 51.62±1.80%. Thecarcasses percentage of local cattle in Ciamis was not significantly different from those of Bali, PO, andcrossbred cattle. The genetic diversity of local cattle in Ciamis was categorized in the medium level, thediversity of local cattle in Tambaksari subpopulation was higher than in Cijulang subpopulation. Localcattle in Ciamis had a closer genetic distance with the PO cattle.
Polymorphism of ADIPOQ and EDG1 genes in Indonesian beef cattle Sutikno, S.; Priyanto, R.; Sumantri, C.; Jakaria, J.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.323-332

Abstract

The ADIPOQ and EDG1 genes were responsible in intramuscular fat deposition and marbling scores. This study was aimed to identify polymorphism of indel g.81966364D>I in promoter region of ADIPOQ gene and SNP c.-312A>G in 5' UTR of EDG1 gene in Indonesian beef cattle. Blood samples were collected from 211 cattle, including Bali (44), Madura (20), Pesisir (18), Katingan (20), PO (22), Pasundan (20), SO (12), Brahman (20), Simmental (15) and Limousin (18). Polymorphism of ADIPOQ gene was analyzed using PCR and direct sequencingmethods, whereas EDG1 gene was analyzed using PCR-RFLP (MscI enzyme) and direct sequencingmethods. Results of genotyping indel g.81966364D>I was monomorphic (DD genotype). The SNP c.-312A>G was polymorphic (AA and AG genotype) in Madura, Pesisir, Pasundan, Brahman, and Limousine. The Frequencies of allele A and G were 0.95, 0.92, 0.98, 0.95, 0.94 and 0.05, 0.08, 0.02, 0.05, 0.06 respectively. The values of Ho and He were 0.05-0.17 and 0.05-0.15 respectively and in Hardy-Weinberg equilibrium (P>0.05). In Bali, Katingan, PO, SO and Simmental were monomorphic (GG genotype). In Bali cattle, two novel SNP candidates were found in position of c.-399C>T and c.-273C>G which were potential to be used as genetic markers of marbling score for Bali cattle. As result this study, it can be concluded that ADIPOQ gene was similar while EDG1 gene was different in Indonesian beef cattle. in addition, found two candidates potential SNP in Bali cattle.
THE DIFFERENTIATION OF SHEEP BREED BASED ON THE BODY MEASUREMENTS Handiwirawan, E.; Noor, R.R.; Sumantri, C.; Subandriyo, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 1 (2011): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.1.1-8

Abstract

This research was carried out to make a difference and to predict genetic distance some sheep thatare genetically related to each other based on the body size measurements. Nineteen bodies size of 323sheeps of five sheep breeds; namely Barbados Black Belly Cross (BC), Garut Local (GL), GarutComposite (GC), Sumatra Composite (SC) and St. Croix Cross (SCC) were measured. Analysis ofvariance and canonical discriminant analysis, Mahalanobis distance, plotting canonical and dendogramwere performed using PROC GLM, PROC CANDISC, PROC CLUSTER and PROC TREE of SASsoftware ver. 9.0. Index Length, Width Slope, Depth Slope, Balance and Cumulative Index of GC sheepwere significantly higher than the four other breeds. Canonical discriminant analysis successfully coulddifferentiate among the five sheep breeds. All five breed were divided into two groups: the first groupconsisted of SCC, SC and BC; and the second group consisted of the GL and GC. The results of geneticdistance estimation showed that the SCC had a value of sheep genetic distance closest to SC (10.83) andBC (27.98), while GL had the closest distance to GC (66.60). The tail width, horn base circumference,horn length (canonical 1) and variable length of the tail and body length (canonical 2) were the breeddifferentiation variable in this study.
Peningkatan produktifitas lebah madu melalui penerapan sistem integrasi dengan kebun kopi Saepudin, R.; Fuah, A. Fuah M.; Sumantri, C.; Abdullah, L.; Hadisoesilo, S.
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 21, No 1 (2011)
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Abstract : The study of relationship between the honey productivity and honey bee-coffee plantation integration was conducted in Kepahiang, the Province of Bengkulu. The objective of this study was to evaluate the application of Apis cerana-coffee plant integration system on honey production and coffee bean as well.. The experiment was arranged in a completely randomized design with two treatments and ten replications. The result showed that honey production was higher by 114% than that outside the plantation. Similar to the honey productionn, coffee been production at honeybee-coffee plantation integration was significantly higher by 10,55 % than that was unpollinated by Apis cerana.Key words: cerana, coffee, integration. production
A New SNPs at 3’UTR Region of calpain 1 gene and its association with growth and meat quality traits in beef cattle Dairoh, D.; Jakaria, J.; Ulum, M. F.; Sumantri, C.
Journal of the Indonesian Tropical Animal Agriculture Vol 47, No 1 (2022): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.47.1.17-28

Abstract

The objective of this study was to evaluate single nucleotide polymorphisms (SNP) in the 3'UTR region of CAPN1 gene by direct sequencing technique in beef cattle and its influence on growth and meat quality of the contrast mode ultrasound image in Bali cattle. The numbers of beef cattle used were Bali cattle (n=52 heads) which compared to purebred Belgian Blue (n=30 heads), Limousine (n=14 heads), Wagyu (n=7 heads), and PO (n=10 heads). Genetic diversity data were obtained from calculations by PopGen 1.32 software. Ultrasound imaging of the thoracic longissimus dorsi muscle in Bali cattle was carried out between the 12 − 13 ribs, and the sonogram was analyzed by Image-J NIH software. The result shown that 3’UTR of CAPN1 gene was found in six discovery SNPs that polymorphic in Bali cattle, they were g.15284 C>T, g.15347 T>G, g.15525 G>A, g.15853 G>A, g.15905 G>A, g.15915 G>A and Indel mutation was polymorphic in Bali cattle, Belgian Blue, Limousine, and PO. There was no association between these SNPs and growth traits. However, SNP g.15525 G>A was significantly associated (P<0.05) with a backfat thickness (BFT) in Bali cattle. In conclusion, the CAPN1 gene in Bali cattle is a candidate for Marker Assisted Selection (MAS) related to meat quality.
Co-Authors A. Anggraeni A. B. L. Ishak, A. B. L. A. Fuah M. Fuah A. GUNAWAN A. Mardiastuti A. Muktiani A. T. Pratiwanggana, A. T. A.B.L. Ishak A.O. Rini ACHMAD FARAJALLAH Ahmadi, J. Amin, M. F. Anneke Anggraeni Anuraga Jayanegara Anwar, P. Ardiyana, M. Arief, I. I. Arif, A. A. Bambang Ngaji Utomo Budiman, C. D. A. Astuti D. Herdiana D. Rahmat D.T.H. Sihombing Dairoh Dairoh, D. Darmati, S. Dewi, M. K. Dudi . E. Andreas E. Damayanthi E. Handiwirawan E.D. Gurnadi F. Saputra, F. Fastawa, R. Furqon, A. Galib, I. Gurnadi, R. E. H. Hidayati, H. H. Martojo H. Nuraini Habiburahman, R. Harahap, R. S. Hardiyanto, D. Hasibuan, H. Hendraningsih, L. I Supriatna I. Arifiantini Indriastuti, R. Jakaria Jakaria Jodiansyah, S. K. JAMAL Khaeruddin Khaeruddin Komarudin L. Abdullah L. Praharani Listyarini, K. Lukmanudin, M. M. Imron M. Yamin Mulyono, R. H. Musyaddad, T. N. Hilmia P. D. M. H. Karti Permatasari, P. R. I. Arifiantini R. Priyanto R. R. Noor, R. R. R. Saepudin R.E. Gurnadi R.R. Noor Rahmadani, R. P. Restu Misrianti Roosita, K. Rosyada, Z. N. A. Rukmiasih S. Darwati S. Hadisoesilo S. Murtini S. Rahayu, S. S. Subandriyo Sari, S. A. Setiadi, D. R. Sopian, Y. Suhita, R. L. Sulistyanto, B. Supriyanto, J. Sutikno Madnasri Syaikhullah, G. T. Maulana, T. T. Sartika T. Suryati Ulum, M. F. Ulupi, N. Widiono, Widiono Yanthi, N. D. Zainatha, F. M. Zamanti, D.