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USE OF MEDIA TYPES AND FERTILIZER TREATMENTS FOR THE GROWTH OF DENDROBIUM ORCHIDS Ida Ayu Meva Lianawati; Ni Putu Adriani Astiti; Ni Luh Suriani
SIMBIOSIS Vol 9 No 1 (2021)
Publisher : Jurusan Biologi Universitas Udayana

ABSTACT Reseach on dendrobium orchids was planted in the media of wood charcoal and coconut fibers and it is needed with Gandasil B fertilizer wood charcoal and coconut fiber media and is needed with Gandasil B and Multitonik fertilizer. This study aims to determine the effect of media and fertilizer on orchid growth and to determine pests and diseases in Dendrobium orchid plants. This reseach was conducted in May until July 2019 in the Plant Physiology Laboratory Biologi Prodi Studi Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana. Qualitative and quantitative data are used that combine the Gandasil B fertilizer and fibers and wood charcoal media Fertilizer Gandasil B fertilizer with concetration G0 (0 g L-1), G2 (20 g L-1), G3 (30 g L-1), G4 (40 g L-1), G5 (50 g L-1). Fertilizer Multitonik M0 (0 g L-1), M1 (1,1 g L-1), M2 (1,2 g L-1), M3 (1,3 g L-1), M4 (1,4 g L-1), M5 (1,5 g L-1). Each fertilizer concentration was repeated 6 times. The results showed the best orchid growth was found in the use of Multitonik fertilizer and the best orchid growth was found in the use of Multitonik number of leavers and root growth were found in the use of coconut fibers media

DEVELOPMENT OF Arenga pinnata Merr SEEDLINGS CULTURED ON MS AND WPM MEDIUM Made Ria Defiani; Ida Ayu Astarini; Eniek Kristiyanti; Ni Luh Suriani
SIMBIOSIS Vol 8 No 1 (2020)
Publisher : Jurusan Biologi Universitas Udayana

Arenga palm (Arenga pinnata) is one species from palmae family. Economically, the palm is suitable for home industry, such as Balinese offering which use parts of young leaf, sap can be used as special local genius sweet drink from Bali, fruits are boilled that can be mixed with sugar for healthy ice. Arenga palm can be propagated aseptically using tissue culture technique. Culture media may varried between MS and WPM with or without adding hormone. Auxin can be added as 2.4-D ( 4 ppm) to induce callus. NAA and BAP with concentration 1.5 ppm respectively also added to induce multiplication of shoots or roots. Each treatment had 10 replicatations. Six weeks after cultured, callus growth observed only at WPM media that enriched with 2,4-D. The others treatment show the explant was dormant because did not show any growth while analysed anatomically under microscope.

SELEKSI JENIS TUMBUHAN PAKAN DAN KANDUNGAN NUTRIEN JENIS TUMBUHAN YANG DIMAKAN SAPI BALI (Bos sondaicus) LEPAS SAPIH DI DAERAH BUKIT BADUNG SELATAN, KABUPATEN BADUNG, BALI I Wayan Heri Dismawan; I Ketut Ginantra; Ni Luh Ni Luh Suriani
SIMBIOSIS Vol 2 No 2 (2014)
Publisher : Jurusan Biologi Universitas Udayana

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This study was conducted to determine the plants species and nutrient content of plantspecies selected by weaning of bali cattle in South Badung Hill, Badung regency, Bali. Studywas conducted in three Grazing unit Bali cattle are Sawangan, Kutuh and Pecatu. InDecember 2013 to February 2014. Determination of the composition of plant species in thehabitat (n?) is determined by the square method. Determination of plants composition in diet(r?) determined by utilization methods. This method is based on the percentage bite mark ofplants species in plot sampling. Nutrient content was analyzed crude protein, energy, calciumand phosphorus. Crude protein determination with a Semi-micro Kjeldahl technique andenergy content by Bomb Calorimeter. Determination of mineral content of calcium (Ca) andPhosphorus (P) by using Atomic Absorption Spectrophotometer (ASS). Tests carried out inthe Laboratory of Nutrition and Forage Analytical Chemistry, University of Udayana. TheJURNAL SIMBIOSIS II (2): 192- 202 ISSN: 2337-7224Jurusan Biologi FMIPA Universitas Udayana September 2014results study showed 32 species of plants available in the habitat, there are nine species ofplants that are selected by weaning bali cattle, that are Desmodium heterofilum, Pleurainterupta, Polygala glomerata, Cyperus rotundus, Desmodium triflorum, Dactylocteniumaegyptium, Portulaca sp. Polygala chinensis, and Panicum eruciforme. Nutrient content ofplant species eaten a protein ranged from 11.25% - 17.14%, GE 3.10 kcal / g - 4.11 kcal / g,Ca 0.33% - 1.86%, and 0.026% P - 0.24%.Keywords: Weaning bali cattle, plants selected species, nutrient content

PEMANFAATAN LIMBAH KULIT BAWANG MERAH (Allium cepa L.) DAN CANGKANG TELUR AYAM UNTUK MENINGKATKAN PRODUKSI TANAMAN SAWI (Brassica rapa var. parachinensis L.) Novita Sari; Made Ria Defiani; Ni Luh Suriani
SIMBIOSIS Vol 10 No 1 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Penelitian bertujuan untuk mengetahui pengaruh ekstrak kulit bawang merah dan cangkar telur ayam terhadap pertumbuhan tanaman sawi (Brassica rapa var. parachinensis L.). Penelitian dilakukan di Laboratorium Fisiologi Tumbuhan dan Geenhouse Progam Studi Biologi, Fakultas MIPA Universitas Udayana. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor yaitu penambahan bahan organik dengan 4 taraf sebagai berikut, perlakuan 1 (Kontrol) disiram dengan air, perlakuan 2 (disiram ekstrak kulit bawang merah 100 ppm), perlakuan 3 (disiram ekstrak cangkang telur ayam 300 ppm), perlakuan 4 (disiram ekstrak cangkang telur ayam 300 ppm + ekstrak kulit bawang merah 100 ppm). Parameter pertumbuhan yang diamati meliputi bobot basah tanaman, bobot kering tanaman, dan jumlah daun. Pengamatan parameter jumlah daun dilakukan setiap 1 minggu sekali. Hasil penelitian menunjukkan pemberian ekstrak kulit bawang merah, cangkang telur ayam serta kombinasi antara kulit bawang merah 100 ppm + cangkang telur ayam 300 ppm berpengaruh nyata terhadap bobot basah total, bobot basah tajuk, bobot kering total, dan bobot kering tajuk.

THE APPLICATION OF ISOLATE LACTIC ACID BACTERIA (LAB) Lactobacillus bulgaricus and Streptococcus thermophilus IN BODY SCRUB PRODUCTION Bayu Putri Handayani; Retno Kawuri; Ni Luh Suriani
SIMBIOSIS Vol 6 No 2 (2018)
Publisher : Jurusan Biologi Universitas Udayana

The majority of body scrub that are distributed in the market contain paraben. Paraben is a type chemicals that are safe to use in the appropriate amount. But it could cause skin irritation. This research was conducted to know the durability from natural body scrub by yogurt and rice flour without chemical preservative and safe for skin. The research methods are comparison of the use of Lactobacillus bulgaricus and Streptococcus thermophilus with concentration 25:25; 30:20; 20:30. The yogurt will be mixed with rice flour and the treatment control using factory yogurt with concentration of rice flour comparing with yogurt namely 10:14; 10:16; 10:18. This product was tested to 50 panelists. This product was kept in room temperature ±28oC for a month and conducted the identification of microbial impurities that contaminate the product with Gram staining method and catalase test. Data was analyzed by analysis of variance (ANOVA). Starter with different comparison 25:25, 30:20; 20:30 are not making real difference. All of the treatment produce yogurt with typical aroma of yogurt, white colored, and soft texture. The formula in K2 (10:16) treatment with the highest average that obtained 3.38 (very like) in color parameter and 3.08 (very like) in texture parameter. Although in aroma parameter the average value that obtained is 2.79 (like). In A1, A2, and B2 treatment had the highest durability percentage that obtained 83,3% and the lowest was shown in K1 and K3 treatment that obtained 0%. Microbial impurities that identified was bacteria from Genus Bacillus and Genus Streptobacillus, fungal impurities from Genus Aspergillus, Rhizopus, and Trichoderma. Keywords : lactic Acid bacteria, yogurt, Lactobacillus bulgaricus, Streptococcus thermophilus, body scrub

UJI KEBERADAAN Staphylococcus aureus PADA SOSIS TRADISIONAL (URUTAN) YANG BEREDAR DI PASAR TRADISIONAL DI DENPASAR, BALI Ni Putu Niti Rahayu; Retno Kawuri; Ni Luh Suriani
SIMBIOSIS Vol 2 No 1 (2014)
Publisher : Jurusan Biologi Universitas Udayana

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The aim of this study is to determine the total amount of Staphylococcus aureus in atraditional sausage (urutan) sold in Denpasar traditional market in Bali. Sausage samples aretaken from four areas in Denpasar (North Denpasar, East Denpasar, West Denpasar, and SouthDenpasar). From each area, three traditional markets are selected and from each market 2merchants are chosen. The calculation of the number of Staphylococcus aureus is done byplatting method and by pour plate method. The results show that the highest number ofStaphylococcus aureus belongs to the North Denpasar region in the mount of 241,067 CFU/gwhile the lowest belongs to the region of West Denpasar with 71,233 CFU/g. These two resultsare significantly different with one another (P<0,05). The maximum limit of microbialcontamination in processed meats (sausages) for Staphylococcus aureus based on IndonesianNational Standard (SNI) is equal to 102 CFU / g. Based on the testing that has been done, allurutan contamination by the Staphylococcus aureus in Denpasar traditional market, hasexceeded the threshold amount of SNI.Keywords : Traditional Sausages (urutan), Staphylococcus aureus

NILAI NUTRISI JENIS-JENIS TUMBUHAN PAKAN RUSA TIMOR (Cervus timorensis) DI PENANGKARAN BANGSING, PUPUAN, TABANAN, BALI Desak Putu Indah Adnyani; I Ketut Ginantra; Ni Luh Suriani
SIMBIOSIS Vol 4, No 1 (2016)
Publisher : Jurusan Biologi Universitas Udayana

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This study aims to determine the selection of plants and nutrient content of plant species selected by Timor deer (Cervus timorensis). This research was conducted in Penangkaran Bangsing, Pupuan, Tabanan, Bali. Determination of the composition of plant species of Timor deer feed (C. timorensis) using the direct method. The species of plants eaten by Timor deer are identified to the species level. The nutrient content of crude protein is determined by Semi-micro Kjeldahl technique, energy content (GE) with Bomb calorimeter, Calcium (Ca) and Phosphorus (P) content by Atomic Absorption Spectrophotometer (ASS) technique. Selection of the plant species eaten using the Ivlev selection index. The nutrient content of plant species is eaten compared to the standard nutrient content for timor deer. The results of the study were 13 species of selected plants, namely Impreata cylindrica, Pennisetum purpureum, Panicum ranusum, Axonopus compressus, Panicum muticum, Spilatnhes paniculata, Mikania micrantha, Gliricidia sepium, Calliandra sp, Hibiscus rosa-sinensis, Sechium edule, Diplazium esculentum, Pogostemon auricularia. The crude protein content ranged from 9.30 - 31.18%, GE 2.724 – 4.446 kcal / g, Ca 0.02 - 0.84%, and P 0.32 to 3.01%. The nutrient content is eaten according to nutritional standards according to Tuckwell (2003). Keywords : timor deer (Cervus timorensis), selection of plant spesies, nutrient content

TYPE OF NUTRIENT FEED DEER TIMOR (Cervus timorensis) IN BREEDING BANGSING, PUPUAN, TABANAN, BALI. Desak Putu Adnyani; I Ketut Ginantra; Ni Luh Suriani
SIMBIOSIS Vol 6 No 2 (2018)
Publisher : Jurusan Biologi Universitas Udayana

This study aims to determine the selection of plants and nutrient content of plant species selected by Timor deer (Cervus timorensis). This research was conducted in Penangkaran Bangsing, Pupuan, Tabanan, Bali. Determination of the composition of plant species of Timor deer feed (C. timorensis) using the direct method. The species of plants eaten by Timor deer are identified to the species level. The nutrient content of crude protein is determined by Semi-micro Kjeldahl technique, energy content (GE) with Bomb calorimeter, Calcium (Ca) and Phosphorus (P) content by Atomic Absorption Spectrophotometer (ASS) technique. Selection of the plant species eaten using the Ivlev selection index. The nutrient content of plant species is eaten compared to the standard nutrient content for timor deer. The results of the study were 13 species of selected plants, namely Impreata cylindrica, Pennisetum purpureum, Panicum ranusum, Axonopus compressus, Panicum muticum, Spilatnhes paniculata, Mikania micrantha, Gliricidia sepium, Calliandra sp, Hibiscus rosa-sinensis, Sechium edule, Diplazium esculentum, Pogostemon auricularia. The crude protein content ranged from 9.30 - 31.18%, GE 2.724 – 4.446 kcal / g, Ca 0.02 - 0.84%, and P 0.32 to 3.01%. The nutrient content is eaten according to nutritional standards according to Tuckwell (2003). Keywords : timor deer (Cervus timorensis), selection of plant spesies, nutrient content

THE POTENTIAL OF CLOVE LEAF EXTRACK (Syzygium aromaticum L.) AGAINST GROWTH OF FUNGAL Rhizoctonia solani Kuhn. CAUSE OF BLIGHT DISEASE IN RICE PLANTS (Oryza sativa L.) I Gusti Ayu Diana Meirani; Ni Made Susun Parwanayoni; Ni Luh Suriani
SIMBIOSIS Vol 11 No 1 (2023)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2023.v11.i01.p04

Rhizoctonia solani Kuhn. is a fungal pathogen that causes sheath blight in rice plants. Sheath blight disease can reduce rice production for the people of Indonesia. To prevent blight, fungicides are used. The continuous use of synthetic fungicides with inappropriate doses can have a negative impact, so it is necessary to look for alternatives with vegetable fungicides, one of which is clove leaf (Syzygium aromaticum L.). The aim of this study was to determine the ability of clove leaf extract to inhibit the fungus R. Solani and the class of compounds contained in the extract. Data were collected and collected using the MIC (Minimum Inhibitory Concentration) test using the well-diffusion method, the inhibition percentage of clove leaf extract using the colony method, and the content of compounds using the phytochemical test. The minimum concentration (MIC) of clove leaf extract capable of inhibiting the fungus R. solani is 0.5%. The percentage of inhibitory activity can be reduced by 100% at an extract concentration of 1.5%. Clove leaf extract contains groups of alkaloids, tannins, flavonoids, saponins, phenols, steroids and terpenoids.

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