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All Journal HAYATI Journal of Biosciences Medical Journal of Indonesia Indonesian Journal of Pharmaceutical Science and Technology Indonesian Journal of Biotechnology Warta IHP (Warta Industri Hasil Pertanian) Indonesian Journal of Chemistry JFIOnline Jurnal Ilmu Kefarmasian Indonesia Jurnal Aisyah : Jurnal Ilmu Kesehatan Jurnal Locus Penelitian dan Pengabdian Journal of Applied Agricultural Science and Technology Pharmaceutical Sciences and Research (PSR) Eduvest - Journal of Universal Studies Ezra Science Bulletin Jurnal Kefarmasian Indonesia
Herman Suryadi
Department Of Pharmacy, Faculty Of Pharmacy, Universitas Indonesia, Jakarta, Indonesia
Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Electrical & Electronics Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : Medical Journal of Indonesia

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Quantitative analysis of lactose and lactulose in urine by high performance liquid chromatography for determination of intestinal lactase activity Dharmasetiawani, Nani; Suryadi, Herman; Firmansyah, Agus
Medical Journal of Indonesia Vol 12, No 1 (2003): January-March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.365 KB) | DOI: 10.13181/mji.v12i1.84

Abstract

Determination of intestinal lactase activity is directly done by measuring its activity in intestinal epithelium. This is an invasive method and ethically can not be done in healthy infants. Indirectly, determination of lactase activity, stated as excretion and ingestion ratio of lactose and lactulose, needs 30 hours hospitalized infants. The aim of this study was to look for a method for determination of lactase activity which is not invasive and not necessary hospitalized. Using this method  lactose and lactulose were given as a single oral load after 2 hours fasting. Urine were collected for 5 hours starting from consuming sugar solution and then lactose and lactulose concentration in the urine were measured by High Performance Liquid Chromatography. The results showed single oral load of lactose and lactulose can be used for determination of lactase activity in infant and the infants were observed only for 7 hours. (Med J Indones 2003; 12: 8-12) Keywords: Lactase activity, Lactulose, Lactose, High Performance Liquid Chromatography
Co-Authors -, Harmita . Azizahwati . Azizahwati . Harmita . Hayun Abdul Mun'ím Agus Firmansyah Ambarsari, Christy Andi Utama ANDI UTAMA Andiri Niza Syarifah Anja Tamabri Arif Arrahman Arif Arrahman Arif Arrahman, Arif Arifayu Addiena Kurniatri Arifianti, Ayun Erwina Arry Yanuar Berna Elya Callista Andinie Mulyadi Chairunissa, Ananda Hanny Dessy Ariyanti Dessy Ariyanti Esti Mumpuni Forestrania, Roshamur Cahyan Fready SIP Fuad, Asrul Muhammad Hayun . Hayun Hayun Hayun Hayun, Hayun Hendri, Rifki Anshory Heria Oktavia Heria Oktavia, Heria Irianti, Marina Ika Jantan, Ibrahim Junaidi, Lukman Kamaruzzaman, Mohd Amir Larosa, Febri LS Lina Elfita Lina Elfita Luh Putu Ratna Sundari Makau, Juliann Nzembi Maksum Radji Maksum Radji Maksum Radji MARIA BINTANG Maryati Kurniadi Maryati Kurniadi Melanie, Yuanki Nafi’ah, Nadya Ismi Nani Dharmasetiawani Ofa Suzanti Betha Partomuan Simanjuntak Prasetio, Nisrina Putri PUSPITA LISDIYANTI Putri Mulia, Ocha Rahmadi, Agus Rahmasari, Ratika Sembiring, Enny Rimita Septiarini, Tri Hastuti Shanti Ratnakomala SHANTI RATNAKOMALA Siburian, Eva Siswa Setyahadi Siswa Setyahadi Sri Wardatun Subagja ., Subagja Sunarsih Sunarsih Susandra Jaya Vicha Vicha Yahdiana Harahap Yahdiana Harahap Yodifta Astiningrum Yodifta Astriningrum Yodifta Astriningrum, Yodifta