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Respon Pertumbuhan dan Hasil Tanaman Padi Sawah akibat Aplikasi Blue Green Algae dan Pupuk N Mieke Rochimi Setiawati; Pujawati Suryatmana
Soilrens Vol 17, No 1 (2019)
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (525.992 KB) | DOI: 10.24198/soilrens.v17i1.23214

Abstract

The growth and yield of rice plants is very dependent on nitrogen (N) fertilizer. N fertilizer applied to wetland rice mostly unutilized due to the lost through the process of denitrification and leaching. The presence of blue-green algae (BGA), procaryotic algae N2-fixing in the paddy field is advantageous to help to uptake the N for plants by direct fixation of N2 from air and then converted into cell proteins. Excess N fixation results were released into the BGA growth environment. BGA biomass at the latter stage will be mineralized to ammonium. Mineralized ammonium from BGA cells as a source of N that can be used directly by paddy plants. This experiment used a randomized block design with factorial pattern; BGA inoculant dose treatment consisted of: 0; 5 kg; 10 kg ha-1 and urea dose 30; 60; 90; 120 kg N ha-1. The results of this experiment showed that the use of BGA can increase the rate of growth of rice plants and reduced urea requirements by 25%. The combination of 90 kg ha-1 N fertilizer with 10 kg ha-1 Blue-green algae inoculant can increase grain yield by 31.47% per hectare (2.71 tons ha-1).
Potensi Tanaman Sorgum (Sorghum bicolor L.), Azotobacter sp. dan Pseudomonas sp. Sebagai agen biologis dalam proses Fitoremediasi Hidrokarbon minyak bumi Pujawati Suryatmana; Alyani Shabrina; Nadia Nuraniya Kamaluddin; Betty Natalie Fitriatin; Reginawanti Hindersah; Mieke Rochimi Setiawati
Soilrens Vol 18, No 1 (2020)
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v18i1.29039

Abstract

Phytoremediation using Sorghum (Sorghum bicolor L.) plant is an alternative green technology to overcome oil polluted soil. Improving of the phytoremediation performance are needed stimulating agent to enhance the rate of hydrocarbon biodegradation and increase the plant growth. One of the bioagent that can act as biostimulant is Azotobacter sp. and Pseudomonas sp., because thouse bioagent can provide elements N and P, and phytohormone for sorghum plants. The purpose of this study is to examine the role of Azotobacter sp. and Pseudomonas sp. as a biostimulant for Sorghum bicolor L. in the process of biodegradation of petroleum hydrocarbon in soil system. The factorial randomized block design was used as the experimental design. The treatment consisted of 4 application levels of Azotobacter sp. and 4 levels of application of Pseudomonas sp. The results showed that there was no interaction between Azotobacter sp. and Pseudomonas sp. aplication on the efficiency of hydrocarbon degradation of the population of Azotobacrer spp., Pseudomonas spp. and plant height increase. Azotobacter sp. and Pseudomonas sp. also did not show an increase in value on each test variable. While, the potential of sorghum plants without the application of Azotobacter sp. and Pseudomonas sp. (control treatment) showed the value of the efficiency of biodegradation in the range of values of 60.442% - 68.165% during 14 weeks period and not significantly different from other treatments.
Karakteristik Azolla pinnata sebagai Pengganti Bahan Pembawa Pupuk Hayati Padat Bakteri Penambat N2 dan Bakteri Pelarut P Mieke Rochimi Setiawati; Pujawati Suryatmana; Amalia Chusnul
Soilrens Vol 15, No 1 (2017)
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (516.511 KB) | DOI: 10.24198/soilrens.v15i1.13346

Abstract

The use of inorganic fertilizers for a long term can reduce soil fertility. One of the alternatives solving this problem is by using solid biofertilizers. Solid biofertilizer requires carrier materials that can support the viability of potential microbial within. Azolla pinnata is one of the carriers that has potential as a substitute for commonly used carrier materials. This study aims to determine the effect of Azolla pinnata and other organic materials with inoculant dosage on viability of bacterial population, N-total, P-total of solid biofertilizer. This research was conducted at Soil Biology Laboratory, Department of Soil Science, Faculty of Agriculture, Padjadjaran University in January-February 2015. The treatments were organic matter (Azolla pinnata, litter compost and peat) with various dosages of bacterial inoculant (20%, 25% 30%). This experiment used the Completely Random Design with factorial pattern. The main observations of the study were analyzed by ANOVA test and continued with Duncan test with 5% confidence level. The results showed that Azolla pinnata carrier has significantly effect to the increase of N-Total and P total of solid biofertilizer. The inoculant dosage of 30% affects the population increase of Azotobacter sp., and endophytic bacteria, but does not effect to the population of Phosphate Solubilizing Bacteria and Azospirillum sp. Azolla pinnata can be recommended as an alternative carrier that can replace peat and litter compost materials.The results of this study was illustrate Azolla pinnata can be recommended as an alternative carrier for solid biofertilizer that can replace peat and litter compost material with 30% inoculant dosage.Keywords: Azolla pinnata compost, litter, peat, solid biofertilizer
Peran Mikroba Dekomposer Selulolitik dari Sarang Rayap dalam Menurunkan Kandungan Selulosa Limbah Pertanian Berselulosa Tinggi Mieke Rochimi Setiawati; Nizar Ulfah; Pujawati Suryatmana; Reginawanti Hindersah
Soilrens Vol 17, No 2 (2019)
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v17i2.26365

Abstract

Agricultural waste is rich in cellulosic material that is difficult to degrade but can be decomposed by cellulase enzymes. Cellulosic material is an organic component abundantly in nature in the form of cellulose and hemicellulose polymer. Utilization of microbes in the process of waste bioconversion can be done to get added value from the waste material into other products such as organic fertilizer. Cellulase-producing microbes can be isolated from various sources, one of which is from termite nests. The use of microbes from termite nests as decomposers for composting high-yielding agricultural waste was carried out in this study. Cellulolytic microbial isolates (combination of bacteria and fungi and without decomposers) were applied to agricultural wastes high in cellulose content: rice straw, bagasse, and oil palm empty fruit bunches. Randomized Block Design was applied and each treatment was and repeated 3 times. The composting process was carried out for 1 month indoors. The results showed that cellulolytic microbial consortium obtained from termite nests reduced the compost cellulose content of bagasse agricultural waste in one month. Whereas rice straw and oil palm empty fruit bunches showed a tendency to decrease cellulose content because cellulose degradation by decomposer microbes still continues after one month of incubation.
Analisis C, N, C/N Ratio Tanah dan Hasil Padi yang Diberi Pupuk Organik dan Pupuk Hayati Berbasis Azolla Pada Lahan Sawah Organik Mieke Rochimi Setiawati; Diyan Herdiyantoro; Maya Damayani; Pujawati Suryatmana
Soilrens Vol 16, No 2 (2018)
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (505.531 KB) | DOI: 10.24198/soilrens.v16i2.20857

Abstract

ABSTRACT Organic farming, especially organic rice requires a continuing supply of organic fertilizer in large quantity as a source of nutrition for rice plants. In long-run, organic system is can retain higher soil organic carbon (SOC), partially compensates the negative impact by improving physical and biological properties, as well as nutrient retention capacity of the soil. Azolla pinnata is an organic nitrogen source rich in protein. The high nitrogen content in A. pinnata is related to the presence of nitrogen-fixing Anabaena azollae microsimbion in A. pinnata leaves. A. pinnata compost is an alternative for biofertilizer carrier that can be used in organic rice fields. The contribution of azolla-based organic farming for organic farmers is to decrease the dependency on the animal manure in cropping season. The field experiment comprised of conventional compost as control and combination of organic fertilizer and biofertilizer with Azolla carrier. Organic fertilizer were 5 ton/hectare (ton/ha), 7.5 ton/ha, and 10 ton/ha combined with 0, 10, 20 kg/ha biofertilizer with Azolla carrier with no addition of inorganic fertilizer. Complete randomized design were used in the experiment which consisted of three replications. This technology produced organic fertilizer better than conventional compost of rice straw and manure made by farmers. Organic fertilizer base with Azolla mixture of 7.5 ton ha-1 plus solid biofertilizer (10 kg/ha) can produce 6.58 ton/ha of dry harvested grain, higher than conventional compost treatment (control). The application of organic fertilizer base on azolla and solid biofertilizers gave the increasing yield prospect of organic rice in Cisayong, Tasikmalaya regency West Java.Keywords : Azolla pinata, carbon, nitrogen, organic farming, rice.
Keragaman Mikroflora, Mikrofauna, Kandungan C-organik, dan Total N Tanah Sawah Akibat Aplikasi Azolla dan Pupuk Hayati Mieke Rochimi Setiawati; Pujawati Suryatmana; Tualar Simarmata
Soilrens Vol 18, No 1 (2020)
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v18i1.29041

Abstract

This study was conducted to determine the effect of Azolla pinnata and biofertilizers (Azotobacter and Phosphate Solubilizing Bacteria) on the total microflora, microfauna, organic C, N-total soil, and C/N ratio of soil in lowland rice. The study was carried out in the SPLPP experimental field (Agricultural Research and Development Research Studio) of the Faculty of Agriculture, Padjadjaran University, Jelekong Village, Ciparay, Bandung Regency, West Java, with an altitude of 628 meters above the sea level. The experimental design used was factorial randomized block design consisted of two factors and repeated four times. The first factor was the provision of Azolla with two levels: without Azolla and 3 tons ha-1 of Azolla pinnata. The second factor was the provision of biofertilizer with three levels, without biofertilizer, 5 L ha-1 biofertilizer, and 10 L ha-1 biofertilizer. The results showed that there was an interaction between A. pinnata with biofertilizer on the increase in the total bacterial population. Total fungus, microfauna (nematodes), and soils N-total content did not increase with the addition of Azolla or biofertilizers. Independently A. pinnata increased soil C-organic matter while biofertilizer 10 L ha-1 increased the total population of actinomycetes.
Potensi Jenis Bahan Organik Sebagai Biostimulan dalam Meningkatkan Populasi Azospirillum sp, dan Hasil Kedelai (Glycine Max. L.) pada Inceptisol Jatinangor Pujawati Suryatmana; Silmi Rahadiana Putri; Nadia Nuraniya Kamaluddin; Mieke Rochimi Setiawati
Soilrens Vol 18, No 1 (2020)
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v18i1.29031

Abstract

Azospirillium is a genus of non-symbiotic N fixer bacteria, known as a biological fertilizer inoculant. The quality of Azospirillum sp. inoculant often decrease when applied outside its original habitat. Therefore, it is necessary to maintain the viability and increase its effectiveness. One way that can be done is by adding organic material as additives as a source of energy for bacteria. Potential organic materials can be used as additives to stimulate the viability, activity and effectiveness of Azospirillium sp. are coconut water, molasses and bran. The organic material contained elements such as C, N and P as the source of nutrients for Azospirillium sp. which was inoculated in Soybean (Glycine max L.) plants. This experiment aimed to examine the effect of additive application on Azospirillum sp. population, root nodules, and seed weight of soybean plants (Glycine max L.) on Jatinangor Inceptisols. The experimental design used was Randomized Block Design (RBD) consisted of combination between of Azospirillum sp. and additives type, namely molasses, coconut water and bran. The results showed that the application of a mixture of bran, molasses, and coconut water attracted the population of Azospirillum sp. higher than the control treatments. Meanwhile molasses, coconut water, and additive mixtures have the potential to increase the number of root nodules and soybean pods yields. Coconut water, molasses and bran are organic materials that have the potential as additives that stimulate the activity and increase the population of Azospirillium sp. and soybean pods.
Regenerasi Berbagai Jenis Eksplan Nilam Klon Sidikalang dan Aplikasi Azotobacter pada Tahap Aklimatisasi Erni Erni; Denny Sobardini Sobarna; Anne Nuraini; Syariful Mubarok; Pujawati Suryatmana; Yudhistari Sihombing; Christine Angel
Agrikultura Vol 27, No 2 (2016): Agustus, 2016
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (636.998 KB) | DOI: 10.24198/agrikultura.v27i2.9992

Abstract

AbstrakKondisi produktivitas nilam di Indonesia saat ini masih rendah, hal ini diduga disebabkan oleh rendahnya penggunaan benih unggul serta kendala ketersediaan benih yang tepat waktu, jumlah bibit yang seragam dan bebas penyakit, serta masih rendahnya mutu genetik tanaman. Sehingga perlu dilakukan upaya perbanyakan maupun peningkatan mutu genetik dengan aplikasi teknologiin vitro. Percobaan I: Pertumbuhan eksplan nilam pada komposisi sitokinin yang berbeda secara in Vitro (Rancangan Acak Lengkap pola faktorial), faktor pertama (jenis ekplan : mata tunas, pucuk, dan daun), sedangkan faktor kedua (0 mg/l sitokinin; 0,5 mg/l BAP; 1,0 mg/l BAP; 0,5 mg/l Zeatin; dan 1,0 mg/l Zeatin). Percobaan II: Multiplikasi stek mikro nilam secara in vitro (Rancangan Acak Lengkap). Percobaan III: Respon stekmikro terhadap inokulasi Azotobacter sp. pada tahap aklimatisasi (Rancangan Acak Kelompok) terdiri dari lima perlakuan (tanpa inokulasi, 1 ml, 2 ml dan 3 ml Azotobacter sp.). Hasil Percobaan I menunjukkan tidak terdapat interaksi antara jenis eksplan dengan jenis dan konsentrasi sitokinin. Penggunaan eksplan pucuk menghasilkan rata-rata jumlah tunas, pertambahan jumlah daun serta rata-rata bobot segar eksplan lebih baik serta penambahan 0,5 mg/l BAP pertambahan jumlah daun dan rata-rata bobot segar eksplan nilam lebih baik pada 8 MST. Percobaan II menunjukkan bahwa penggunaan 0,01 mg/l NAA dan 1 mg/l BAP merupakan perlakuan yang terbaik untuk multiplikasi tunas nilam pada peubah jumlah tunas dan jumlah daun. Percobaan III menunjukkan inokulasi Azotobacter sp. dengan dosis 3 ml memberikan rata-rata pertambahan jumlah daun, jumlah akar dan panjang tanaman relatif lebih baik.Kata kunci : Aklimatisasi, Azotobacter, Cytokinin, Eksplan, Nilam, Regenerasi
Aktivitas Enzim Fosfatase dan Ketersediaan Fosfat Tanah pada Sistem Tumpangsari Tanaman Pangan dan Jati (Tectona grandis L.f.) setelah Aplikasi Pupuk Hayati Betty N. Fitriatin; Reginawanti Hindersah; Pujawati Suryatmana
Agrikultura Vol 19, No 3 (2008): Desember, 2008
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.025 KB) | DOI: 10.24198/agrikultura.v19i3.995

Abstract

Pupuk hayati seperti mikrob pelarut fosfat (MPF) dan fungi mikoriza arbuskula (FMA) berperan dalam siklus unsur hara fosfor di dalam tanah. Tujuan penelitian ini adalah mengetahui aktivitas enzim fosfatase dan ketersediaan fosfat di tanah Ultisols pada sistem tumpangsari jati (Tectona grandis L.f.) dengan tanaman pangan setelah aplikasi pupuk hayati berupa FMA dan MPF.  Penelitian menggunakan rancangan acak kelompok pola faktorial dengan faktor pertama adalah pupuk hayati (tanpa dan dengan 2 ton ha-1, 4 ton ha-1, dan  6 ton ha-1 pupuk hayati), dan faktor kedua adalah pola tanam tumpang sari dengan jati (jati dengan jagung, jati dengan kedelai, dan jati dengan jagung dan kedelai).        Hasil penelitian menunjukkan tidak terdapat interaksi antara pupuk hayati  dengan pola tumpang sari terhadap aktivitas fosfatase, fosfat tersedia dan populasi MPF. Aplikasi pupuk hayati meningkatkan  aktivitas fosfatase Ultisols dan mempengaruhi  perubahan populasi MPF, namun tidak mempengaruhi kandungan fosfat tersedia tanah. Aktivitas fosfatase tanah lebih tinggi pada pola tumpang sari jati dengan jagung dan kedelai dibandingkan dengan tumpang sari jati dengan jagung atau kedelai saja.  Pola tanam tumpang sari jati dengan tanaman pangan tidak mempengaruhi fosfat tersedia dan populasi MPF di dalam tanah.
Aplikasi Bakteri Endofitik Penambat N2 untuk meningkatkan Populasi Bakteri Endofitik dan Hasil Tanaman Padi Sawah Mieke Rochimi Setiawati; Dedeh H. Arief; Pujawati Suryatmana; Ridha Hudaya
Agrikultura Vol 19, No 3 (2008): Desember, 2008
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.21 KB) | DOI: 10.24198/agrikultura.v19i3.1009

Abstract

Bakteri endofitik penambat nitrogen (N2) yang bersimbiosis dengan tanaman padi dapat digunakan sebagai pupuk hayati karena dapat memberikan nitrogen yang ditambatnya langsung pada tanaman. Penelitian pot ini bertujuan untuk mendapatkan konsentrasi dan teknik aplikasi pupuk hayati cair bakteri endofitik penambat N2 yang tepat dalam meningkatkan populasi bakteri endofitik penambat N2 dan  hasil tanaman padi sawah.  Percobaan dirancang dalam Rancangan Acak Kelompok yang menguji faktor konsentrasi pupuk hayati (25, 50, 75 dan 100 ml l-1 pupuk hayati) dan metode aplikasi pupuk (Perendaman, Penyemprotan dan kombinasinya) . Hasil percobaan memperlihatkan adanya peningkatan populasi bakteri endofitik penambat N2 di daun, batang, dan akar setelah aplikasi pupuk hayati baik melalui teknik perendaman, penyemprotan maupun kombinasinya. Aplikasi  100 ml l-1 pupuk hayati melalui semua teknik menghasilkan gabah kering panen 85,89 g tanaman-1, lebih besar  7,79 %  daripada penggunaan 50 ml l-1 pupuk hayati.
Co-Authors . WISJNUPRAPTO Adawiah, Aliya Zahrah Ade Setiawan Aditya, Fasa Aditya, Fasa Aditya, Fasa Agung Karuniawan Agung Muhammad Yusuf Aliya Zahrah Adawiah Alyani Shabrina Amalia Chusnul Anas Ramdhani Andi Hana Mufidah Elmirasari Anne Nuraini Anne Nurbaity Anni Yuniarti Annisa Rosalina Sylvia Annisa Rosalina Sylvia Apong Sandrawati Asmiran, Priyanka Aten Komarya Bagus Adityo Bang, Sunbaek Benny Joy Betty N. Fitriatin Betty Natalie Fitriatin Budiman, Muhammad Nafariz Chotimah, Andina Christine Angel Damayani, Maya Dedeh H. Arief Dedi Ruswandi Denny Sobardini Sobarna Denny Sobardini Sobarna Dewi, Yeni Wispa Dewikusuma Ikhsani Adhiningtyas Diyah Sri Utami Diyan Herdiyantoro Djuwansah, Muhamad Rachman EDWAN KARDENA Eka Safitri Emma Trinurani Sofyan Enny Ratnaningsih Erni Erni Erni Suminar Eso Solihin Evi Entang Fatimah Evi Entang Fatimah Fadhilla Oktavianingtyas Trisilvi Fajri Syahid Nurhakim Febby Nur Indriani Fera Siti Meilani Fiky Yulianto Wicaksono Gordon Pius Marihot Halimah, Ummu ‘Azizah Handayani, Sri Harry Rum Haryadi Ibnu Rizki Perdana Ikrar Nusantara Putra Ikrar Nusantara Putra Ilyas, Ichsan Indra Herliana Iskariman, Ahlan Azman Iva Fitriani Jajang Sauman Hamdani Januar, Dodi Ganjar Jihan Fitria Meilani Khumairah, Fiqriah Hanum Latifah, Tipah Leoni Silvia Lia Nur Linda Maharani, Nadhira Saniya Mahdi Argawan Putra Mahfud Arifin Marenda Ishak Sonjaya Sule Masruri, Muhammad Kholil Maulana, Nurzen Maya Damayani Maya Damayanti Meddy Rachmadi Mieke Rochimi S Mieke Rochimi Setiawati Mieke Rochimi Setyawati Muhammad Agus Mulyana Muhammad Amir Solihin Nadia N. Kamaluddin Nadia Nuraniya Kamaluddin Nanda Aditya Setyawan Nandha Afrilandha NENNY NURLAENY Ninda Meiditia Putri Nizar Ulfah Nugraha, Gita Bina Nugraha, Gita Bina Nugraha, Gita Bina Nurullita Fitri Qurnia Oviyanti Mulyani Pirda Nurhopipah Probo Condrosari Rachelita, Nadia Rainaldi, Rainaldi Rara Rahmatika Risanti Reginawanti Hindersah Reginawati Hindersah Rhazista Noviardi Rhazista Noviardi Rhazista Noviardi Richard A. Gunawan Ridha Hudaya Rija Sudirja Rina Devnita Risanti, Rara Rahmatika Risanti, Rara Rahmatika Salsabilla, Camilla Sandra Amalia Riyadi Sandrawati, Apong Santi Rosniawaty Setiawati, Mieke Richimi Shinta Nurrizqi Indrayani Silmi Rahadiana Putri Sumadi Sumadi Suswanto, Irwan syafrizal Syafrizal Syafrizal Syafrizal Syafrizal Syariful Mubarok Syifa Nabila Kurnia Sylvia, Annisa Rosalina Tarra Martiana Dewi Triyani Dewi Tualar Simarmata Ummu ‘Azizah Halimah Uum Umiyati Vera Oktavia Subardja Vera Oktavia Subarja Wati, Dyah Aditya Yori Tridendra Yudhistari Sihombing Yuliati Machfud Yusup Hidayat Zahra Ilmiyati Zellya Handyman Zulkifliani Zulkifliani Zulkifliani Zulkifliani, Zulkifliani