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The Antibacterial Activity of Bajakah Tampala Extracts (Spatholobus littoralis Hassk.) Mouthwash Formulation Inhibited Dental Plaque against Streptococcus mutans Rikhaturhohmah; Rospadila Dwi Adrila; Widiya Dwi Handayani; Rasyani; Ananda Alifvia Suprapto; Nofran Putra Pratama; Mitsalina Fildzah Arifah
Journal of Food and Pharmaceutical Sciences Vol 12, No 2 (2024): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.15147

Abstract

Dental plaque is the main cause of dental caries caused by Streptococcus mutans, with a high prevalence in Indonesia. Currently, the mouthwash market contains high levels of alcohol, which can cause long-term side effects. Tampala bajakah root (Spatholobus littoralis) is used in traditional medicine for the Dayak community in Central Kalimantan. Bajakah Tampala root has antibacterial activity produced by flavonoids and phenolic compounds. The development of herbal cosmetics can be achieved by Bajakah Tampala mouthwash formulations to prevent dental plaque caused by Streptococcus mutans infection. In this study, ultrasound-assisted extraction (UBT) and infusion (IBT) derived the active compounds of Bajakah Tampala root extract. The various concentrations of UBT (20–80%) and IBT (10%) were evaluated for antibacterial activity using the disk diffusion method. The results showed that positive control and 80% UBT have antibacterial activity higher than other extracts, with an inhibition zone of 14,01±2,70 mm. Based on these results, an effective mouthwash dosage formulation can be developed at 80% UBT. The formulation evaluation of mouthwash assessed viscosity, homogeneity, pH, and organoleptic test. The UBT mouthwash product has qualified formulation evaluation parameters. This research contributed to the innovation of herbal cosmetics by developing the potential of Indonesian medicinal plants. Keywords: Bajakah Tampala, Antibacterial activity, Mouthwash, Herbal cosmetic, Streptococcus mutans.
Pengaruh Pemberian Informasi Terhadap Pengetahuan Masyarkat Dusun Turi Tentang TOGA Nofran Putra Pratama; Sari, Kurnia Rahayu Purnomo; Rahayu, Budi
The Journal of Innovation in Community Empowerment Vol 5 No 2 (2023): Journal of Innovation in Community Empowerment (JICE)
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jice.v5i2.968

Abstract

I Peningkatan pembangunan kesehatan menjadi prioritas utama sebagai tolak ukur kemajuan suatu negara. Apoteker adalah salah satu agen tenaga kesehatan yang memiliki peran dalam pembangunan kesehatan, salah satunya dengan melakukan edukasi-edukasi kepada masyarakat seperti pengenalan khasiat bahan alam dalam pengobatan yang dikemas dalam bentuk Tanaman Obat Keluarga atau TOGA yang disertai dengan swamedikasi. TOGA dapat menjadi alternatif pengobatan keluarga yang aman karena bahan yang berasal dari alam. Obat yang berasal dari bahan alam dianggap memiliki efek samping yang lebih kecil, mudah diolah dan dikonsumsi untuk terapi alternatif pada penyakit-penyakit tertentu. Penggunaan TOGA akan lebih baik jika masyarakat juga paham terkait swamedikasi sehingga keberhasilan pengobatan akan semakin tinggi. Banyakanya manfaat dari TOGA berbanding terbalik dengan realita yang ada terutama di Dusun Turi, warga disana banyak yang tidak mengetahui TOGA dan hanya sebagian yang mengetahui tetapi tidak paham bagaimana penggunaanya. Tujuan dari kegiatan ini yaitu memberikan informasi mengenai khasiat dari berbagai jenis TOGA dan cara pengelolaannya TOGA menjadi sehingga menjadi TOGA dengan kualitas yang baik serta mewujudkan masyarakat sehat di Dusun Turi dengan mensinergiskan pengetahuan tentang TOGA dan swamedikasi. Metode yang digunakan dalam pengabdian ini antara lain ceramah, praktik, dan diskusi tanya jawab. Langkah-langkah kegiatan ini melalui beberapa tahapan yaitu tahap persiapan, tahap pelaksanaan, dan tahap evaluasi pelaporan. Dari kegiatan tersebut didapat indikator capaian yaitu pemahaman masyarakat mengenai khasiat dari berbagai jenis TOGA dan cara pengelolaannya TOGA. Kegiatan pengabdian kepada masyarakat ini berjalan dengan baik terbukti dengan banyaknya peserta yang aktif bertanya mengenai materi yang disampaikan. Hasil dari kegiatan ini adalah terjadi peningkatan pengetahuan peserta mengenai TOGA dari 71,43% menjadi 92,86%.
Peningkatan Peningkatan Pengetahuan Masyarakat Terhadap Diare Dan Swamediasinya Lutfi Hidiyaningtyas; Pratama, Nofran Putra
The Journal of Innovation in Community Empowerment Vol 6 No 1 (2024): Journal of Innovation in Community Empowerment (JICE)
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jice.v6i1.1238

Abstract

. Angka kesakitan penyakit diare meningkat dari tahun ke tahun. Penderita diare di D.I. Yogyakarta (DIY) tergolong tinggi. Dari data Laporan pelaksanaan Tugas Tahunan TA 2022, jumlah penderita diare yang ditangani sesuai standar di Kabupaten Sleman pada tahun 2022 sebanyak 24,15%, cakupan ini terbilang rendah meskipun sudah mengalami peningkatan dari tahun 2021 sebesar 12,23%. Swamedikasi merupakan salah satu tindakan dalam mengobati penyakit yang dialami dengan cara pemilihan serta penggunaan obat oleh seseorang tanpa resep dokter. Menurut Badan Pusat Statistik (BPS), persentase masyarakat yang melakukan swamedikasi meningkat dari 80,68% pada tahun 2021, menjadi 82,74% pada tahun 2022. Swamedikasi yang dilakukan secara tidak tepat dan benar dapat menimbulkan berbagai masalah pengobatan, timbul penyakit baru karena efek samping obat, dan peningkatan biaya pengobatan akibat penggunaan obat yang tidak rasional. Berdasarkan hal tersebut perlu dilakukan pemberian informasi yang mendukung dalam pelaksanaan swamedikasi. Kegiatan ini bertujuan untuk meningkatkan pengetahuan masyarakat demi meningkatkan kualitas hidup untuk mencegah terjadinya komplikasi akibat penanganan diare yang kurang adekuat. Sasaran kegiatan ini adalah warga Pedukuhan Rejodani, Kecamatan Ngaglik, Sleman. Evaluasi yang dilakukan melalui perbandingan nilai pretest dan posttest. Berdasarkan hasil analisisi pemberian informasi melalui kegiatan penyuluhan/sosialisasi dapat meningkatkan pengetahuan masyarakat dari 86% menjadi 96,19% tentang diare dan swamedikasinya.
Daya Hambat Fraksi n-heksan, Etil Asetat, dan Air dari Ekstrak Etanol Bunga Cengkeh (Syzygium aromaticum (L.) Merrill & Perry) Terhadap Bakteri Staphylococcus epidermidis Ratih Armay Gustari; Nofran Putra Pratama; Kurnia Rahayu Purnomo Sari
JOURNAL OF PHARMACEUTICAL (JOP) Vol. 1 No. 1 (2023): Journal of Pharmaceutical (JOP)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) UNIVERSITAS JENDERAL ACHMAD YANI YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jop.v1i1.865

Abstract

Background: Clove flower plants are known as traditional plants that can be used in food, drink and medicine. Clove flower ethanol extract (Syzygium aromaticum (L.) Merrill & Perry) has antibacterial activity. Antibacterial is a substance that can interfere with the growth or kill bacteria by interfering with the metabolism of harmful microbes.Objective: This study was conducted to determine how effective the n-hexane, ethyl acetate, and water fractions from clove the ethanol extract of clove flower (Syzygium aromaticum (L.) Merrill &Perry) in inhibiting the growth of Staphylococcus epidermidis bacteria.Method: Extract were made by the maceration method with 70% ethanol as a solvent. Then proceed with the fractionation process using a liquid-liquid extraction method using three solvents, namely n-hexane solvent (non-polar), ethyl acetate solvent (semi-polar), and water solvent (polar). Identification of clove flower extract and fraction flower was carried out by organoleptic test, phytochemical screening test, and thin layer chromatography test. A Bacterial inhibition test was carried out using the Kirby-Bauer disc diffusion method using concentrations of 20%, 40%, 60%, 80% and 100% with positive control used chloramphenicol while the negative control used distilled water.Result: The results of the n-hexane and ethyl acetate fraction had bacterial inhibition at concentrations of 60%, 80%, and 100%. The water fraction had a zone of bacterial inhibition at 100% concentration.Conclusion: Based on the results of the study was found that the n-hexane, ethyl acetate, and water fractions had effectiveness in inhibiting the growth of Staphylococcus epidermidis bacteria.
Peredaman Radikal Bebas Ekstrak Etanol Daun Rosella (Hibiscus sabdariffa L) Dengan Metode Frap (Ferric Reducing Antioxidant Power) Vina Farah Fauziah; Devika Nurhasanah; Nofran Putra Pratama
JOURNAL OF PHARMACEUTICAL (JOP) Vol. 1 No. 1 (2023): Journal of Pharmaceutical (JOP)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) UNIVERSITAS JENDERAL ACHMAD YANI YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jop.v1i1.866

Abstract

Background: Antioxidant are chemical compounds that can donate electrons to unpaired free radicals, thereby reducing the effects of free radical oxidation. One of the plants that have antioxidants activity is the rosella plant, for example in the leaves. Rosella leaves contain phytochemical compounds that are useful as antioxidant, namely flavonoids, alkaloids, saponins, tanin.Objective: Determine the antioxidant activity of the ethanol extract of rosella leave (Hibiscus sabdariffa L) by FRAP method.Method: Rosella leaf was macerated using 70% ethanol as solvent. Then filtered and thicken using a frying pan on an electric stove over medium heat of approximately 300 watt. Phytochemical screening was carried out, namely flavonoids, alkaloids, saponins, tanin and TLC test to indicate the presence of flavonoid compounds as antioxidants. Quantitative analysis was carried out to determine antioxidant activity with the FRAP method measured using a UV-Vis spectrophotometer.Result: The antioxidant activity test of the ethanol extract of rosella leaves has FRAP value of 1,749 mmol FeSO4/mg. Based on statistical analysis T-test, shows that there is a difference between the sample and the standard with a significant value (p<0,05) is 0,001.Conclusion: The antioxidant activity test on rosella leaves with the FRAP method proved that the ethanol extract of rosella leaves had antioxidant content with a FRAP value of 1,749 mmol FeSO4/mg.
Aktivitas Peredaman Ekstrak Daun Pandan Wangi (Pandanus amaryllifolius) dengan Metode DPPH (2,2-difenil-1-pikrilhidrazil) Nurhasanah, Devika; Nofran Putra Pratama; Sri Purwa Pujihastuti
JOURNAL OF PHARMACEUTICAL (JOP) Vol. 1 No. 2 (2023): Journal of Pharmaceutical (JOP)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) UNIVERSITAS JENDERAL ACHMAD YANI YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jop.v1i2.1223

Abstract

Background: Antioxidants are compounds that prevent an oxidation reaction from free radical compounds and important role in capturing free radicals in the human body. Excessive free radicals in the body can cause tissue damage and degenerative diseases. Pandanus amarillyfolius are known to act as natural antioxidant that have the potential to capture free radical compounds, because of their presence of flavonoid compounds. Objective: This study aims to determine the free radical scavenging activity of DPPH against the leaf extract of Pandanus amaryllifolius. Method: Pandanus amaryllifolius extraction was carried out using the maceration method with methanol as solvent (1:10). Concentration was carried out using a rotary evaporator to obtain a thick extract. Phytochemical screening and TLC test were carried out qualitatively using the mobile phase n-butanol: acetic acid: water (6:2:2) and phytochemical testing including flavonoids, alkaloids, saponins, tannins, steroids or terpenoids to determine the presence of flavonoid compounds as antioxidants. Free radical scavenging activity was tested using DPPH free radical scavenger with concentrations of vitamin C 2, 4, 5, 6, 8 ppm and pandanus fragrance extract concentrations of 10, 15, 20, 25 ppm and measured using a UV-Vis spectrophotometer to calculate the IC50 value. Result: The results of the phytochemical screening test of fragrant pandan indicate the presence of flavonoid compounds. The Rf value of the TLC test obtained 0.765 quarcetin and 0.787 extract and the resulting yellow spots, the Rf value of pandan extract which was almost the same as the Rf value of quercetin indicated that the pandan extract may contain the same compounds as quercetin, namely flavonoid compounds. The results of the free radical scavenging activity of methanol extract of pandan leaves were 86,861 g/ml and for comparison, vitamin C was 39,103 g/ml. Based on the statistical analysis of the T-Test, it is known that there is a difference between the sample and the standard with a significant value (p <0.05), namely 0.001 and 0.023. Conclusion: The Pandanus amaryllifolius extract test using the DPPH method is in the strong category (IC50 <50 -100 g/ml) and the comparison of Vitamin C is in the very strong category (IC50 <50 g/ml).
Pengaruh Perbedaan Pelarut Dalam Ekstraksi Herba Seledri (Apium graveolens L.) Terhadap Aktivitas Peredaman Radikal Bebas DPPH Luluk, Luluk nurjanah; Pratama, Nofran Putra; Sari, Kurnia Rahayu Purnomo
JOURNAL OF PHARMACEUTICAL (JOP) Vol. 1 No. 2 (2023): Journal of Pharmaceutical (JOP)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) UNIVERSITAS JENDERAL ACHMAD YANI YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jop.v1i2.1225

Abstract

Background: The decreased physiological function is influenced by age and degenerative diseases. Degenerative diseases can be caused by free radicals. Neutralization of free radicals can be done by giving antioxidants. However, synthetic antioxidants cause many side effects so that natural antioxidants are needed from natural ingredients. Celery (Apium graveolens L.) is a natural antioxidant that can be used as antioxidant because celery contains flavonoid compounds. Objective: This study aims to determine the effect of different solvents of ethanol 96%, ethyl acetate, and n-hexane in the extraction of celery herbs on the free radical scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH). Method: Celery herbs were extracted using the maceration method to obtain ethanol 96%, ethyl acetate, and n-hexane extracts. The extract was then tested for its free radical scavenging activity using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method using UV-Vis spectrophotometry. The principle of the DPPH method is that a hydrogen atom binds to a free radical, causing the free radical to become a non-radical. Result: The results showed that 96% ethanol extract of celery had the highest antioxidant activity with an IC50 value of 4.084 g/mL, followed by ethyl acetate 15.250 g/mL, and n-hexane 28.206 g/mL. Conclusion: which the three solvents could be categorized as very active.
Uji Aktivitas Antibakteri Fraksi N-Heksana Daun Pepaya (Carica papaya Linn.) Terhadap Escherichia coli dan Staphylococcus aureus Aprillia, Hananing; Putra Pratama, Nofran; Nur'Aini Purnamaningsih
JOURNAL OF PHARMACEUTICAL (JOP) Vol. 1 No. 2 (2023): Journal of Pharmaceutical (JOP)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) UNIVERSITAS JENDERAL ACHMAD YANI YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jop.v1i2.1231

Abstract

Background: Infectious diseases are still a problem for the world of health. Infectious diseases are caused by viral, bacterial and parasitic infections. Inappropriate use of antibiotics in treating bacterial infections can cause antibiotic resistance effects. Prevention of the effects of resistance can be done by looking for natural antibiotic agents derived from plants. One of the potential medicinal plants as antibiotics is papaya leaf. Objective: The objective of study was determine the antibacterial activity of the papaya leaf n-hexane fraction against Escherichia coli and Staphylococcus aureus and to determine the diameter zone of inhibition the papaya leaf n-hexane fraction at 20%; 40%; 60%; 80%; and 100% against Escherichia coli and Staphylococcus aureus. Method: Papaya leaf were extraction by using maceration method of 70% ethanol as solvent. Separation of compounds based on the level of polarity using fractionation method with n-hexane: water (1:1 v/v) solvent then carried out phytochemical screening and separation of compounds using TLC method. The antibacterial activity testing used by agar diffusion method Kirby Bauer with the treatment group of papaya leaf n-hexane fraction with a concentration of 20%; 40%; 60%; 80%; and 100%, the positive control group used Ampicillin 10 ?g and the negative control group used Aquades. Result: Diameter zone of inhibition Escherichia coli and Staphylococcus aureus was smallest at 20% was 6,57 ± 0,15 mm and 6,69 ± 0,33 mm, while the diameter zone of inhibition the largest at 100% was 7,92 ± 0,23 mm and 7,94 ± 0,16 mm. Conclusion: The papaya leaf n-hexane fraction concentration of 20%; 40%; 60%; 80%; and 100% had antibacterial activity against Escherichia coli and Staphylococcus aureus. The higher concentration then the larger diameter zone of inhibition formed.
Uji Aktivitas Antibakteri Ekstrak Etanol Sereh Wangi (Cymbopogon nardus L. Rendle) Terhadap Pertumbuhan Bakteri Staphylococcus aureus ATCC 25923 Menggunakan Metode Sumuran Leswara, Dianita Febrina; Enggrainia, Bella; Pratama, Nofran Putra
JOURNAL OF PHARMACEUTICAL (JOP) Vol. 2 No. 1 (2024): Journal of Pharmaceutical (JOP)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) UNIVERSITAS JENDERAL ACHMAD YANI YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jop.v2i1.1442

Abstract

Background: Bacteria are one of the organisms that cause infectious diseases. One of the bacteria that can cause infection is Staphylococcus aureus. Treatment of bacterial infections is by using antibiotics, but using the antibiotics for a long term can cause resistance. It is necessary looking for alternative compounds that can inhibit the growth of Staphylococcus aureus. Cymbopogon nardus L. Rendle is a plant that has antibacterial compound, that compounds are flavonoids and tannins.Objective: To determine the antibacterial activity of Cymbopogon nardus ethanol extract against the growth of Staphylococcus aureus ATCC 25923 using the well method.Method: Cymbopogon nardus is extracted using the maceration method with 70% ethanol solvent. A phytochemical screening test was carried out and continued with an antibacterial activity test using the well method with concentrations of 5%, 10%, 15% and 20%.Results: Phytochemical screening show that Cymbopogon nardus ethanol extract contains saponins, flavonoids, steroids and tannins. The results of the antibacterial test showed that the average inhibitory zone value of Cymbopogon nardus ethanol extract at concentrations of 10%, 15% and 20% was 23.00; 23.77; 24.96 mm, whereas at concentration of 5% it did not show an inhibition zone for the Staphylococcus aureus ATCC 259323 bacteria.Conclusion: Cymbopogon nardus ethanol extract has antibacterial activity against the growth of Staphylococcus aureus ATCC 259323 with a minimum inhibitory concentration (MIC) which is able to inhibit the growth of Staphylococcus aureus ATCC 259323 at concentration of 10%, for concentrations of 10%, 15% and 20% it has an inhibitory power of very strong category.
Pengaruh Variasi Waktu Ekstraksi terhadap Aktivitas Peredaman Radikal Bebas DPPH Ekstrak Biji Kopi Robusta Lampung Barat Arifah, Mitsalina Fildzah; Rusydan, Azka Muhammad; Sayyidah Yahya, Fina Ummu; Pratama , Nofran Putra; Saputra, Diky Permana
JOURNAL OF PHARMACEUTICAL (JOP) Vol. 2 No. 2 (2024): Journal of Pharmaceutical (JOP)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) UNIVERSITAS JENDERAL ACHMAD YANI YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jop.v2i2.1454

Abstract

Background: Robusta Coffee (Coffea canephora) has been cultivated in Indonesia, especially in the West Lampung area. Robusta coffee contains secondary metabolites that act as antioxidants, namely chlorogenic acid and other phenolic components. These secondary metabolites of robusta coffee bean can be obtained using the extraction method. The ultrasound-assisted extraction (UAE) is a well-known method to be more effective and lower temperature than other extraction methods. Variations in time impacted by the antioxidant activity of coffee bean extract. Research objective: To determine the effect of variation in extraction time on the free radical reduction activity of DPPH from West Lampung Robusta coffee bean extract. Research method: Robusta coffee beans were extracted by the UAE method using a 70% ethanol solvent (1:100). The variation in extraction time was 10, 20, and 30 minutes. Robusta coffee bean extract was subjected to organoleptic tests, phytochemical screening, and antioxidant activity in DPPH free radical scavenging. Research results: The yield results at 10, 20, and 30 minutes of robusta coffee bean extract were 24.04%, 35.71%, and 20.15%. The results of the organoleptic test of the extract produce a thick texture, brown color, and unique aroma. The results of the phytochemical screening of the extract contain alkaloids, phenolics, tannins, and flavonoids.  The IC50 values at the time variations of 10, 20, and 30 minutes, and quercetin were 29.97 ± 2.737 ppm; 30.5 ± 1.745 ppm; 23.31 ± 4.638 ppm; and 1.42 ± 0.440 ppm respectively, which were significantly different. Conclusion: The variation in extraction time affected the free radical reduction activity of DPPH was an extraction time of 30 minutes in the most optimal DPPH free radical scavenging activity