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Journal : Ranah Research : Journal of Multidisciplinary Research and Development

Deteksi Kontaminasi Toxoplasma gondii Pada Daging Kambing di Bali dengan Primer BAG1 Santoso, Jemima Lewi; Adrianto, Hebert; Tandean, Victor Setiawan; Yuwono, Victor Kurniawan; Santoso, Novian Budi; Juliasih, Ni Njoman; Sayogo, William; Bravimasta, Bracovanca Diwayestara; Firmanto, Ian Ardhiya; Afifah, Balqis
Ranah Research : Journal of Multidisciplinary Research and Development Vol. 7 No. 6 (2025): Ranah Research : Journal Of Multidisciplinary Research and Development
Publisher : Dinasti Research

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38035/rrj.v7i6.1710

Abstract

Toxoplasmosis is an infection caused by the protozoan parasite Toxoplasma gondii. One of the transmission routes of T. gondii infection is the consumption of undercooked meat. Goats are an important source of income for farmers in Bali and have been reported to have the potential to carry T. gondii. The aim of this study was to detect the presence of Toxoplasma gondii contamination in goat meat in Kuta and Ubud, Bali, using molecular methods with BAG1 primers. Meat samples obtained from markets in Kuta and Ubud were sectioned, followed by extraction of T. gondii DNA, amplified by PCR, and electrophoresed on a 1% agarose gel. The resulting amplification products were approximately 470 base pairs in size. The study results indicated that goat meat was only successfully obtained from the Kuta market. Examination for the presence of T. gondii parasite in goat meat from the Kuta market targeting the BAG1 gene yielded all negative results (100%). Hygiene in goat husbandry should be maintained and even improved. Meat sold in markets or stores must be routinely inspected to ensure safety. Additionally, meat should be cooked at sufficiently high temperatures, and consumption of undercooked or rare meat should be avoided
Co-Authors Abigail Elsha Tiara Susanto Achmad Wahdi Ade Lia Ramadani Ade Lia Ramadani Adellita Ilmas Bintang Mohamad Afifah, Balqis Agung Cahyono Alvionita Muntholib Alya Rachmanita, Fathiya Ananda Nabilla Setyani Aren Lestyani Billy Daniel Mesaakh Billy Daniel Messakh Bimo Sasono Bravimasta, Bracovanca Diwayestara Charles Siahaan Chindy Claudya Angriyanto Cindy Clara Sari Clarissa, Regina Dominikus Raditya Atmaka Donny Arintha Putra Gabriella Dyah Saputri, Arini Eko Budi Koendhori, Eko Budi Eko Budi Santoso Elizabeth Sulastri Nugraheni Eppy Setiyowati Eric Dino Tandoyo Etha Rambung Fasyicha Lisana Nur Ilmiyah Fathoni Syahda , Almer Firmanto, Ian Ardhiya Gonaldy, Vincent Aurelius Hanna Tabita Hasianna Silitonga Hebert Adrianto Imelda Ritunga Indrasari, Setyarina Ira Trio Wahyuni Ivana Velda Jemima Lewi Santoso Jonathan Loody Lukas Justine Thiotansen , Keith Kevin Cerwyn Dedwydd Minarni Wartiningsih Muhammad Amin Rais Najmul Laila Nathania Disa Ariesta Andriani Novian Budi Santoso Nuha, Zakiyathun Nurul Illahi Muhammad Risha Nyoto Nyoto Priyo Susilo Putri, Firda Aulia Putu Gede Julian Yuan Pratama Renny Mareta Sari Reny Mareta Sari Reny Mareta Sari Reny Mareta Sari, Reny Mareta Rifka Irhamna Risa Mauludia Ronald Torang Marsahala Panggabean Sayogo, William Semita, I Nyoman Silitonga, Hanna Singgih Singgih Soegeng Soegijanto Sugiharto Tanzilia, May Fanny Tari Kupula, Tiara Tatas H.P. Brotosudarmo Teguh Hari Sucipto, Teguh Hari Timothy Tobias Umi Hanik Umi Hanik Victor Kurniawan Yuwono Victor Setiawan Tandean Widiya Hari Kurnia Yuniati, Evi