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Aktifitas Antioksidan dan Inhibitor Tirosinase Beberapa Stilbenoid dari Tumbuhan Moraceae dan Dipterocarpaceae yang Potensial untuk Bahan Kosmetik Didin Mujahidin; Euis Holisotan Hakim; Yana Maolana Syah; Lia Dewi Juliawati
Jurnal Matematika & Sains Vol 13, No 2 (2008)
Publisher : Institut Teknologi Bandung

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Abstract

In a drug discovery program to combat various diseases, many natural chemicals originating from Indonesian tropicalplants including Moraceae and Dipterocarpaceae have been investigated in our laboratory. These family of plants areknown as rich sources of stilbenoid type of compounds which have been shown to have various bioactivities, that arepotentially important in cosmetic bioindustry. In the course of our search for bioactive natural products from plants, wewere interested in the stilbenoid constituents of these plants. Thus, as part of this program, several stilbene derivativeswere successfully isolated from extracts of Morus macroura, a Moraceous plant locally named andalas, includingoxyresveratrol (1), lunularin (2) and new resveratrol dimers, namely andalasin A (3) and andalasin B (4). While,biotransformation of oxyresveratrol (1) using cell suspension culture of Solanum mammosum affordedtrans-oxyresveratrol-4-O-β-D-glucopyranoside (5). Similarly, some resveratrol derivatives have been isolated fromDipterocarpaceous plants, such as Shorea assamica, S. seminis, Vatica umbonata, and Dryobalanops oblongifolia.These species gave, among others, resveratrol (6), resveratrol-12-C-β-glucopyranoside (7), the resveratrol dimers(-)-ε-viniferin (8) and the new compound diptoindonesin A (9), in addition to resveratrol trimers (-)-α-viniferin (10), thenew compounds cis-diptoindonesin B (11) and trans-diptoindonesin B (12). Among these compounds oxyresveratrol (1),andalasin B (4), trans-oxyresveratrol-4-O-β-D-glucopyranoside (5), resveratrol (6), cis-diptoindonesin B (11) andtrans-diptoindonesin B (12) exhibited the most potent antioxidant activity [1,1-diphenyl-2-picrylhydrazil (DPPH)radical-scavenging activity]; moreover, andalasin A (3) have been reported to show a very strong tirosinase inhibitoractivity, thus valuable in cosmetic industry.
Novel Mutations in the katG Gene in Isoniazid Resistant Mycobacterium tuberculosis Isolate Purkan Purkan; Ihsanawati Ihsanawati; Debbie Soefie Soefie; Yana Maolana Syah; Achmad Saifuddin Noer; Dessy Natalia
Jurnal Matematika & Sains Vol 18, No 1 (2013)
Publisher : Institut Teknologi Bandung

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Abstract

Isoniazid (INH) resistance in Mycobacterium tuberculosis is commonly associated with mutations in katG encoding catalase-peroxidase.  A clinical isolate of M. tuberculosis L21 exhibits isoniazid resistant phenotype.  A 2.2 kb DNA fragment of katG gene from the L21 isolate had been cloned and characterized.  The L21 katG gene has four point mutations, namely T898C; G1388T; T1997C; C2035T; and one G2203 deletion.  The in silico translation analysis of the katG mutant suggests that the mutation results in a truncated protein lacking of 62 C-terminal amino acids with three amino acid substitutions, Trp300Arg, Arg463Leu, and Ile666Thr. The structural model superposition of the mutant protein with the full length protein suggests that the mutant losses an ability to form a dimer structure, and also posseses a distortion of intermolecular interactions in the substrate binding channel. Keywords: katG gene, KatG, MDR M. tuberculosis, Isoniazid resistance, Structure model.   Beberapa Mutasi Baru pada Gen katG pada Isolat Mycobacterium tuberculosis Resisten Isoniazid Abstrak Resistensi Mycobacterium tuberculosis terhadap isoniazid umumnya berkaitan dengan mutasi yang terjadi pada gen katG yang pengode katalase-peroksidase.  Isolat klinis M. tuberculosis L21 fenotip resisten terhadap isoniazid.  Fragmen DNA berukuran 2,2 kb dari gen katG dari isolate L21 telah diisolasi dan dikarakterisasi. Gen katG dari isolat L21 memiliki empat mutasi, yaitu T898C; G1388T; T1997C; C2035T; dan delesi G2203.  Analisis in silico terhadap gen katG mutan menunjukkan bahwa mutasi yang terjadi menhasilkan protein mutan yang kehilangan 62 asam amino pada ujung-C yang juga mengandung perubahan asam amino Trp300Arg, Arg463Leu, dan Ile666Thr. Superposisi model struktur protein antara protein mutan dengan protein utuh menyarankan bahwa protein mutan diduga tidak dapat membentuk struktur dimer dan juga mengandung interaksi molekular pada poket pengikatan substrat yang berbeda. Kata kunci: Gen katG, M. tuberculosis, Resisten isoniazid, Model struktur.
Synthesis of quinine N-oxide and an NMR tutorial in its structure determination Aisyah, Aisyah; Tamaela, Nila Berghuis; Santoso, Joko; Syah, Yana Maolana; Mujahidin, Didin
Jurnal Penelitian Teh dan Kina Vol 17, No 1 (2014)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (637.555 KB) | DOI: 10.22302/pptk.jur.jptk.v17i1.45

Abstract

Cinchona alkaloids are one of abundant natural chiral pool for organocatalysts. The application of an N-oxide group containing molecule in asymmetric reaction is currently wide developed. The structure of quinine consist two amine groups that could be oxidized to the N-oxide groups at N-1’ of quinoline and at N-1 of quinuclidine moieties. The oxidation reaction took place selectively at quinuclidine moiety by controlling the concentration of oxidizing agents. In this study we showed that N-1-oxide quinine could be occurred chemo­selectively by using a low concentration of ozone thana palladium catalyzed oxidation. The structure of products N1-oxide quinine was elucidated by spectroscopy data including 1H-NMR, 13C-NMR, 2D-NMR, infra-red and mass spectrometry. The developed method is a chemoselectively and eco-friendly method for synthesis N1-oxide quinine.
AKTIVITAS ANTIBAKTERI DARI FRAKSI ARTOCARPUS INTEGER (Thunb.) Merr. DENGAN METODE DIFUSI AGAR (Antibacterial Activity of Artocarpus Integer (thunb.) Merr. Fraction by Difusi Agar Method). Zakaria, Zakaria; Soekamto, Nunuk Hariani; syah, Yana Maolana; Firdaus, Firdaus
Jurnal Industri Hasil Perkebunan Vol 12, No 2 (2017): Jurnal Industri Hasil Perkebunan Volume 12 No. 2 Desember 2017
Publisher : Balai Besar Industri Hasil Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (328.107 KB) | DOI: 10.1111/jihp.v12i2.1771

Abstract

 Artocarpus integer (Thunb.) Merr. (Cempedak) is a plant with a kind of Moraceae family that is widely cultivated in Luwu, South Sulawesi. Extraction and antibacterial bioactivity test of Artocarpus integer (Thunb.) Merr. Heartwood have been done. Preparation of heartwood conducted by shaved, dried, crushed and macerated with methanol to obtain a crude extract of methanol. The methanol extract then partitioned successively in n-hexane, chloroform and ethyl acetate to obtain the fraction of n-hexane, chloroform, ethyl acetate, and methanol. Antibacterial activity test using agar diffusion method on two types of gram-negative bacteria (E. coli and S. typhi) and two types of gram-positive bacteria (S. aureus and S. pneumonia) has been performed. The inhibition zone obtained on each fraction are the n-hexane fraction of 12.80 mm in the bacterium S. pneumonia, chloroform fractions of 13.80 mm in the bacterium S. pneumoniae, ethyl acetate fraction of 9.80 mm in bacteria    S. typhi, and the methanol fraction at 12.90 on S. pneumonia bacteria. The chloroform fraction has potential as antibacterial caused by the highest of inhibition ability toward S. pneumonia. Keywords: Antibacterial activity, Artocarpus integer (Thunb.) Merr., MoraceaeABSTRAK Artocarpus integer (Thunb.) Merr. (Cempedak) merupakan tanaman famili Moraceae yang banyak dibudidayakan di Luwu, Sulawesi Selatan. Ekstraksi dan uji bioaktivitas antibakteri kayu batang Artocarpus integer (Thunb.) Merr. telah dilakukan. Kayu batang diserut, dikeringkan, dihaluskan, dan dimaserasi dengan metanol hingga diperoleh ekstrak kasar metanol. Ekstrak metanol kemudian dipartisi berturut-turut dalam n-heksan, kloroform, dan etil asetat sehingga diperoleh fraksi n-heksan, kloroform, etil asetat, dan metanol. Uji aktivitas antibakteri dengan metode difusi agar menggunakan 2 bakteri gram negatif (E. coli dan S. typhi) dan 2 gram positif (S. aureus dan S. pneumonia). Zona hambat terbesar yang diperoleh masing-masing fraksi adalah fraksi n-heksan sebesar 12,80 mm pada bakteri S. pneumonia, fraksi kloroform sebesar 13,80 mm pada bakteri S. pneumonia, fraksi etil asetat sebesar 9,80 mm pada bakteri S. typhi, dan fraksi metanol sebesar 12,90 pada bakteri S. pneumonia. Fraksi kloroform berpotensi kuat sebagai antibakteri karena memiliki daya hambat terbesar terhadap S. pneumonia. Kata Kunci: Aktivitas Antibakteri, Artocarpus integer (Thunb.) Merr., Moraceae
Dua Dimer Resveratrol dari Kulit Batang Shorea parvifolia (dipterocarpaceae) Rosyidah, Kholifatu; Juliawati, Lia Dewi; Syah, Yana Maolana; Hakim, Euis Holishotan; Achmad, Sjamsul Arifin; Makmur, Lukman
Jurnal Natur Indonesia Vol 12, No 2 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (52.681 KB) | DOI: 10.31258/jnat.12.2.163-166

Abstract

Two resveratrol dimers, (-)-ampelopsin F dan (-)-laevifonol were isolated from aceton extract of the stem bark ofShorea parvifolia. The structures of these compounds were determined based on their spectroscopic data includingspectroscopy ultra violet (UV), infra red (IR), nuclear magnetic resonance (1H-NMR, 13C-NMR) and also were comparedto the reported data.
Senyawa Morusin dari Tumbuhan Murbei Hitam (Morus nigra) Ferlinahayati Ferlinahayati; Euis Holisotan Hakim; Yana Maolana Syah; Lia Dewi Juliawaty
Jurnal Penelitian Sains Vol 15, No 2 (2012)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (584.537 KB) | DOI: 10.56064/jps.v15i2.98

Abstract

Tumbuhan murbei hitam merupakan salah satu spesies dari genus Morus. Tumbuhan dari genus ini telah dilaporkan kaya akan senyawa turunan fenol seperti flavonoid, 2-arilbenzofuran dan stilben. Dalam rangka pencarian senyawa turunan fenol dari tumbuhan murbei Indonesia maka telah berhasil diisolasi suatu senyawa flavon terprenilasi yaitu morusin dari ekstrak metanol kayu batang murbei hitam (M. nigra). Struktur senyawa tersebut telah ditetapkan berdasarkan data-data spektroskopi yang meliputi spektrum UV dan IR serta membandingkan KLTnya dengan senyawa morusin standar.
A Cytotoxic Flavanone from The Pod Peels of Theprosia vogelii Hook.f. Valentina Adimurti Kusumaningtyas; Nia Sri Hardianti; Melina Melina; Lia Dewi Juliawaty; Yana Maolana Syah
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 6, No. 2, November 2020
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v6i2.17551

Abstract

Tephrosia vogelii Hook.f. is a species of the family Fabaceae (Leguminoceae). These plants are termed ″Polong-polongan″ in Indonesia, and are known to contain active flavonoid groups. Previous studies have shown the isolation of one known flavanone: isolonchocarpin from methanol extract, and the structure obtained was established based on chemical evidence as well as spectroscopic methods, including NMR, and also by a comparison with published data. This research is aimed at evaluating the cytotoxic property of methanol extract against larvae of Arthemia salina Leach, using the Brine Shrimp Lethality Test (BSLT) method. The results show potent cytotoxicity at LC50 of 41.40 ppm.
Turunan Senyawa Flavonoid dari Daun Macaranga involucrata (Roxb.) Baill dari Buton Tengah, Sulawesi Tenggara Edi Ilimu; Yana Maolana Syah
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 5, No. 1, May 2019
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1030.18 KB) | DOI: 10.15408/jkv.v5i1.7909

Abstract

Macaranga merupakan salah satu genus terbesar dari famili Euphorbiaceae yang terdiri dari 300 spesies dengan nama lokal mahang-mahangan. Tumbuhan Macaranga tersebar luas di wilayah Afrika dan Madagaskar di bagian barat hingga ke wilayah tropis Asia, Australia utara dan kepulauan Pasifik. Di Indonesia tumbuhan Macaranga tersebar di beberapa daerah yaitu daerah Papua, Maluku, Sulawesi, Kalimantan, Sumatera, Bangka, dan Jawa. Kajian fitokimia beberapa spesies Macaranga menunjukan adanya kelompok senyawa fenolik yaitu turunan flavonoid dan stilben, serta turunan terpenoid. Senyawa turunan fenolik tersebut memiliki keunikan dari struktur molekulnya, yaitu adanya subtituen tambahan dari metabolit terpenoid yaitu prenil (C5), geranil (C10), farnesil (C15), dan geranilgeranil (C20). Pada penelitian ini telah dilakukan isolasi metabolit sekunder dari daun M. involucrata (Roxb.) Baill dengan metode maserasi menggunakan pelarut aseton, kemudian dilanjutkan pemisahan dan pemurnian dengan menggunakan kromatografi cair vakum dan kromatografi radial untuk mendapatkan senyawa murni. Penentuan struktur dilakukan berdasarkan analisis data spektrum NMR 1D (1H-NMR dan13C-NMR), NMR 2D (NOESY, TOCSY, HSQC, dan HMBC), dan spektrum massa (MS). Berdasarkan metodologi tersebut, dua senyawa turunan flavon yaitu 5,7,4’-trihidroksi-3’(3-metilbut-2-enil)-3-metoksiflavon (1) dan makarangin (2), telah berhasil diisolasi dari tumbuhan ini. Berdasarkan hasil penelitian tersebut menunjukan daun M. involucrata (Roxb.) Baill yang berasal dari Kabupaten Buton Tengah, Sulawesi Tenggara menghasilkan senyawa fenolik turunan flavonoid. Kata kunci: Euphorbiaceae, Macaranga involucrata (Roxb.) Baill, flavon. Macaranga is one of the largest genera of the family Euphorbiaceae comprising 300 species with local name “mahang-mahangan”. Macaranga is widespread in the region of Africa and the west of Madagascar to the tropical regions of Asia, northern Australia, and the Pacific islands. In Indonesia Macaranga spread in several areas:  Papua, Maluku, Sulawesi, Kalimantan, Sumatra, Bangka, and Java. Phytochemical studies showed the presence of several phenolic compounds such as flavonoids and stilbene derivatives. The phenolic compounds have a unique molecular structure with the addition of some substituents such as prenyl (C5), geranyl (C10), farnesyl (C15), and geranylgeranyl (C20). This research has been conducted on the isolation of secondary metabolites from the leaves of M. involucrata (Roxb.) Baill by maceration method using acetone, followed by separation and purification by using liquid vacuum chromatography and radial chromatography to obtain pure compounds. Determination of the structure is based on data analysis of 1D NMR spectrum (1H-NMR and 13C-NMR), 2D NMR (1H-1HCOSY, NOESY, TOCSY, HSQC, and HMBC), and mass spectra (MS). Based on this methodology, two flavone derivatives 5,7,4'-trihydroxy-3'(3-methylbut-2-enyl)-3-methoxy flavone (1) and macarangin (2), have been isolated from this plant. Based on these results showed that leaf of M. involucrata (Roxb.) Baill from Central Buton, Southeast Sulawesi produces phenolic compounds from flavonoid derivatives. Keywords: Euphorbiaceae, Macaranga involucrata (Roxb.) Baill, flavone.
AKTIVITAS ANTIBAKTERI DARI FRAKSI ARTOCARPUS INTEGER (Thunb.) Merr. DENGAN METODE DIFUSI AGAR (Antibacterial Activity of Artocarpus Integer (thunb.) Merr. Fraction by Difusi Agar Method). Zakaria Zakaria; Nunuk Hariani Soekamto; Yana Maolana syah; Firdaus Firdaus
Jurnal Industri Hasil Perkebunan Vol 12, No 2 (2017): Jurnal Industri Hasil Perkebunan Volume 12 No. 2 Desember 2017
Publisher : Balai Besar Industri Hasil Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (328.107 KB) | DOI: 10.33104/jihp.v12i2.1771

Abstract

 Artocarpus integer (Thunb.) Merr. (Cempedak) is a plant with a kind of Moraceae family that is widely cultivated in Luwu, South Sulawesi. Extraction and antibacterial bioactivity test of Artocarpus integer (Thunb.) Merr. Heartwood have been done. Preparation of heartwood conducted by shaved, dried, crushed and macerated with methanol to obtain a crude extract of methanol. The methanol extract then partitioned successively in n-hexane, chloroform and ethyl acetate to obtain the fraction of n-hexane, chloroform, ethyl acetate, and methanol. Antibacterial activity test using agar diffusion method on two types of gram-negative bacteria (E. coli and S. typhi) and two types of gram-positive bacteria (S. aureus and S. pneumonia) has been performed. The inhibition zone obtained on each fraction are the n-hexane fraction of 12.80 mm in the bacterium S. pneumonia, chloroform fractions of 13.80 mm in the bacterium S. pneumoniae, ethyl acetate fraction of 9.80 mm in bacteria    S. typhi, and the methanol fraction at 12.90 on S. pneumonia bacteria. The chloroform fraction has potential as antibacterial caused by the highest of inhibition ability toward S. pneumonia. Keywords: Antibacterial activity, Artocarpus integer (Thunb.) Merr., MoraceaeABSTRAK Artocarpus integer (Thunb.) Merr. (Cempedak) merupakan tanaman famili Moraceae yang banyak dibudidayakan di Luwu, Sulawesi Selatan. Ekstraksi dan uji bioaktivitas antibakteri kayu batang Artocarpus integer (Thunb.) Merr. telah dilakukan. Kayu batang diserut, dikeringkan, dihaluskan, dan dimaserasi dengan metanol hingga diperoleh ekstrak kasar metanol. Ekstrak metanol kemudian dipartisi berturut-turut dalam n-heksan, kloroform, dan etil asetat sehingga diperoleh fraksi n-heksan, kloroform, etil asetat, dan metanol. Uji aktivitas antibakteri dengan metode difusi agar menggunakan 2 bakteri gram negatif (E. coli dan S. typhi) dan 2 gram positif (S. aureus dan S. pneumonia). Zona hambat terbesar yang diperoleh masing-masing fraksi adalah fraksi n-heksan sebesar 12,80 mm pada bakteri S. pneumonia, fraksi kloroform sebesar 13,80 mm pada bakteri S. pneumonia, fraksi etil asetat sebesar 9,80 mm pada bakteri S. typhi, dan fraksi metanol sebesar 12,90 pada bakteri S. pneumonia. Fraksi kloroform berpotensi kuat sebagai antibakteri karena memiliki daya hambat terbesar terhadap S. pneumonia. Kata Kunci: Aktivitas Antibakteri, Artocarpus integer (Thunb.) Merr., Moraceae
DFT Study on the Reaction Mechanism of Cyclization of 2-Hydroxy Chalcone Catalyzed by Bronsted Acid with M06-2X Functional Suci Zulaikha Hildayani; Muhamad Abdulkadir Martoprawiro; Yana Maolana Syah
Bulletin of Chemical Reaction Engineering & Catalysis 2021: BCREC Volume 16 Issue 4 Year 2021 (December 2021)
Publisher : Department of Chemical Engineering - Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.9767/bcrec.16.4.11487.796-803

Abstract

Flavanones are one of the flavonoid group that has wide variety of applications such as a precursors in drug discovery. In the laboratory, flavanone is often synthesized from chalcone compounds. The conversion of chalcone to flavanone can be catalyzed by bronsted acid. The reaction mechanism for this process is proposed through the Michael addition reaction, however, the energetic details and the rate determining step for this reaction is not certainly known. This research aimed to investigate the reaction mechanism for chalcone-flavanone conversion with the present of bronsted acid as catalyst and also studied the effect of the solvent on the reaction energy profile with computational method. In this study, the modeling of the reaction mechanism for the said reaction was carried out using the DFT computational method with M06-2X functional. The computation was done both in the gas phase and in present of the solvent effect using the PCM models. The results showed that the mechanism of chalcone-flavanone conversion occurred in three steps which are protonation, cyclization, and then tautomerization. Based on these calculations, the rate determining step was the tautomerization reaction, which exhibited the same results with or without the solvent effects. Copyright © 2021 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0). 
Co-Authors A.A. Ketut Agung Cahyawan W Achmad Saifuddin Noer Affrida Affrida Aisyah Aisyah Aisyah Aisyah Alan Jefferson Aliefman Hakim Asep Aripin Asep Kadarohman Azzania Fibriani Bambang Ariwahjoedi Buchari Buchari Buchari Buchari Bunbun Bundjali Deana Wahyuningrum Debbie Soefie Soefie Dessy Natalia Devitri Amisa Dian Nugraheni Didin - Mujahidin Didin Mujahidin Didin Mujahidin Didin Mujahidin Didin Mujahidin Didin Mujahidin Didin Mujahidin Didin Mujahidin Dikhi Firmansyah Eliza Eliza Elvira Hermawati Elvira Hermawati Emilia L Ghisalberti Emilia L. Ghisalberti Emilio L Ghisalberti Endang Rosdiana Eri Bachtiar Eri Bakhtiar Euis Holishotan Hakim Euis Holisotan Hakim Euis Holisotan Hakim Euis Holisotan Hakim Euis Holisotan Hakim Euis Holisotan Hakim Euis Holisotan Hakim Euis Holisotan Hakim Euis Holisotan Hakim Euis Holisotasn Hakim Feraliana Feraliana Ferlinahayati Ferlinahayati Firdaus, Firdaus Hanapi Usman Hartiwi Diastuti Hartiwi Diastuti Hartiwi Diastuti Haryoto Haryoto Hendra Helmanto Hernandi Sujono Hiromitsu Takayama Hiromitsu Takayama I Dewa Agung Panji Dwipayana I Nyoman Adi Winata Ihsanawati Ihsanawati Ikram M. Said Ilim Ilim Ilimu, Edi Iqbal M. Choudhary Jalifah Latip Jalifah Latip Joko Santoso Kholifatu Rosyidah Laily Bin Din Lia Dewi Juliawati Lia Dewi Juliawati Lia Dewi Juliawaty Lia Dewi Juliawaty Lia Dewi Juliawaty Liliasari Liliasari Lukman Makmur Lukman Makmur Lukman Makmur Lukman Makmur Lukman Makmur Lukman Makmur M I Choudhary Marc Jeannin Mariko Katajima Mariko Kitajima Mariko Kitajima Marlia Singgih Marlia Singgih Marlia Singgih Masatake Niwa Melina Melina Muhamad Abdulkadir Martoprawiro Muhammad N. Jalaluddin Muhtadi Muhtadi Nanik Siti Aminah Nia Sri Hardianti Nila Berghuis Tamaela Nila Berghuis Tamaela, Nila Berghuis Nizar Happyana Nizar Happyana Nordin Hj Lajis Norio Aimi Norio Aimi Norio Aimi Noviany Noviany Nunuk H Soekamto Nunuk Hariani Soekamto Purkan Purkan Reza Aditama Rusjdi Tamin Sadijah Achmad Sahidin . Sahidin Sahidin Senadi Budiman Siti Nurbayti Siti Zubaidah Sjamsul Arifin Achmad Sjamsul Arifin Achmad Sjamsul Arifin Achmad Sjamsul Arifin Achmad Sjamsul Arifin Achmad Sjamsul Arifin Achmad Sjamsul Arifin Achmad Sjamsul Arifin Achmad Soerya Dewi Marliyana Sri Atun Suci Zulaikha Hildayani Sukrido Sukrido Suwandri Suwandri Suwandri Suwandri Tia Setiawati Tjodi Harlim Tri Cahyanto Valentina Adimurti Valentina Adimurti Kusumaningtyas Wasinton Simanjuntak Windayani, Neneng Yaya Rukayadi Yuliana, Trisna Zakaria Zakaria