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All Journal Jurnal Teknologi Dan Industri Pangan Biospecies Jurnal Penelitian Pendidikan IPA (JPPIPA) Idaarah: Jurnal Manajemen Pendidikan Bubungan Tinggi: Jurnal Pengabdian Masyarakat Jurnal Biosense BiosciED: Journal of Biological Science and Education Proceeding Biology Education Conference BIOMA : Jurnal Biologi dan Pembelajarannya Jurnal Ilmiah Ilmu Terapan Universitas Jambi BIO PALEMBANICA
Titin Purnaningsih
Program Studi Pendidikan Biologi, FKIP, Universitas Palangka Raya
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Engineering Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Other

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Optimization of in Vitro Culture of Taro Banana (Musa paradisiaca var. sapientum L.) Based on Thidiazuron: A Foundation for Developing a Biotechnology Booklet to Enhance Decision-Making Skills Purnaningsih, Titin; Kimeni; Gunawan, Yohannes Edi; Akhmadi; Nuriman; Adam, Chaidir; Silvita; Fahrina, Ririn; Rahmi, Ennike Gusti
Jurnal Penelitian Pendidikan IPA Vol 11 No 12 (2025): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i12.13368

Abstract

This study aims to optimize the concentration of Thidiazuron (TDZ) in the in vitro culture of taro banana (Musa paradisiaca var. sapientum L.) and to integrate the results as a foundation for developing a biotechnology booklet to enhance decision-making skills. The research was conducted using an experimental method with various TDZ concentration treatments, observing parameters such as the number of shoots and leaves produced. To bridge the gap between biotechnological research and its application in education, this study investigates the optimization of Thidiazuron (TDZ) concentrations for the in vitro multiplication of taro banana (Musa paradisiaca var. sapientum L.). The results showed that the use of TDZ effectively induced shoot formation, with the optimal concentration obtained at 0.01 mg/L TDZ, producing an average of 22.25 shoots and 24.25 leaves. In addition to generating biological data, each stage of this study reflected the implementation of decision-making skills, including problem identification, information gathering, criteria determination, solution selection, and result evaluation. These findings serve as the foundation for developing a biotechnology booklet as a contextual learning medium that not only facilitates the understanding of biotechnology concepts but also trains students to make scientific and reflective decisions.
ISOLATION OF Micractinium Pusillum FRESENIUS FROM FISH PONDS USING SDE TECHNIQUES: SEDIMENTATION, DILUTION, AND ENRICHMENT Adam, Chaidir; Haryono, Agus; Purnaningsih, Titin; Araina, Elga; Mashabhi, Sugeng; Velina, Yessy; Fatiqin, Awalul
Jurnal Ilmiah Ilmu Terapan Universitas Jambi Vol. 9 No. 2 (2025): Volume 9, Nomor 2, June 2025
Publisher : LPPM Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jiituj.v9i2.32929

Abstract

Green water in fish ponds, caused by algal blooms, harbors a diverse array of microalgae species and is commonly observed in aquaculture settings. This resource-rich water source holds promise for research focused on microalgae cultivation at a laboratory scale, serving as a valuable starter sample for such investigations. Preliminary observations suggested that the predominant species in such green water habitat belonged to the genus Micractinium Fresenius 1858. An effective isolation technique of this microalgae species is necessary, not only to reduce the contamination of the rotifers but also to purify the starter cultures. Although automated microalgae isolation techniques have been developed recently, such as using Flow Cytometry via Cell Sorting, traditional isolation techniques are still relevant. One of the traditional microalgae isolation techniques that has been widely used for many years is the dilution technique. This study aims to isolate Micractinium pusillum Fresenius from fish ponds using the modified dilution technique: SDE (sedimentation, dilution, and enrichment). The dilution results showed that rotifer contamination was reduced at a dilution of 10─3 and the density of microalgae was also reduced. At this dilution level, only one type of microalgae was observed, i.e., Micractinium pusillum Fresenius. which was then cultured for enrichment using a simple photobioreactor. This 10-3 culture was observed to grow well during the enrichment stage for 10 days. These results indicate that the SDE isolation technique can be effectively used to isolate microalgae from green water, especially for Micractinium pusillum which is the most abundant microalgae species in green water in this study.
Co-Authors Adam, Chaidir AGUS HARYONO Akhmadi Akhmadi Akhmadi Anggoro, Rahayu Opi Anton Rahmadi Araina, Elga Decenly Decenly Decenly Edy Prasetyo Ennike Gusti Rahmi Fahrina, Ririn Fatiqin, Awalul Gunawan, Yohannes Edi Hartanti, Rio Eka Desi Purwandari Hasanah, Rizka Ipnatul Abdiah Irena Stephani Ambarita Karina Evaying Kimeni Kusuma Putri, Rahmadyah Liswara Neneng Maria Elisha Otis Mashabhi, Sugeng Maya Dewi Sukarno Miranda, Yula Miranda, Yula Nuriman Otis, Maria Elisha Rahayu Opi Anggoro Rahmadyah Kusuma Putri Rahmadyah Kusuma Putri Rahmawati, Rahmawati Riky Riky Rizka Hasanah Silvita Siti Sunariyati Sri Puryaningsih Sugeng Mashabhi Syafutra, Randy Wijaya, Nuriman Yessy Velina