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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of Innovation and Applied Technology Research Journal of Life Science Plantropica: Journal of Agricultural Science AGRIVITA, Journal of Agricultural Science Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education Cakrawala: Jurnal Litbang Kebijakan Jurnal Entomologi Indonesia Jurnal HPT (Hama Penyakit Tumbuhan) Journal of Fisheries & Marine Jurnal Pengabdian Masyarakat SAPANGAMBEI MANOKTOK HITEI
Hagus Tarno
Jurusan Hama Dan Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Social Sciences Other

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Journal : Research Journal of Life Science

 1   2 
Eating Behavior of Imago Aulacophora similis Oliver on Cucumber (Cucumis sativus L.) with Treatment Plant Growth Promoting Rhizobacteria (PGPR) Dwi Wulandari; Luqman Qurata Aini; Hagus Tarno
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.10

Abstract

The cucumber harvests failure occurred mainly due to the destruction of crops by the leaf-eating beetle A. similis Oliver. Many ways are taken especially pesticides that are synthesized widely because it is considered the fastest and most potent to overcome pest disorders. Based on, one of the control techniques that can be used is PGPR, which is a group of bacteria that can be utilized to strengthen plants against pest attacks and plant diseases, so research needs to be done by using this technique to change the feeding behavior of the A. similis Oliver beetle on cucumber plants. The research was conducted at the Green House Department of Pest and Plant Diseases University of Brawijaya Malang in September 2018 to March 2019. It was conducted using Randomized Block Design (RBD) 5 treatment, i.e., PGPR, urea fertilizer and control (without treatment). Based on the results of the study shows that: Application of PGPR on cucumber plant can reduce damage on either leaves or flower due to the eating behavior of imago A. similis Oliver and Application of PGPR can increase the cucumber plant growth during the eating behavior of imago A. similis Oliver.
Fungal Endophytic Beauveria bassiana in Chinese Kale Against Plutella xylostella (L.) Larvae Afandhi, Aminudin; Ikawati, Silvi; Baroro, Ismatul; Afiyanti, Mufidah; Sari, Rose Novita; Handoko, Handoko; Suyono, Tri; Setiawan, Yogo; Tarno, Hagus
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.4

Abstract

This study evaluated three methods of inoculation of Beauveria bassiana endophytes from Chinese kale and then infected into Plutella xylostella L to determine the mortality rate. The endophytic B. bassiana using three different inoculation methods including seeds-soaking, leaf-spraying, and soil-wetting. The Chinese kale seeds from Winsa variety, B.bassiana isolates derived from collection planthopper. The endophytic fungus was identified to be B. bassiana based on the analysis of colony morphology. The results of this study included the degree of colonization of Chinese kale plants and the mortality of P. xylostella. The average B. bassiana colonies in leaves were 13.89%, stems 2.77% and roots 5.55%. The results showed on leaves that the seeds-soaking method obtained higher colonization of B. bassiana rate when compared to leaf-spraying and soil-wetting. The highest percentage of P. xylostella mortality was generated from seeds-soaking (45%), soil-wetting (37.5%), leaf-spraying (35%). The B. bassiana fungus which caused the fastest LT50 with a seeds-soaking (409.48 hours), and then soil-wetting (679.15 hours), leaf-spraying (1090.21 hours). The positive effects of seeds-soaking of endophytic B. bassiana and mortality of Plutella xylostell.
Colony Growth, Sporulation, and Viability of Entomopathogenic Fungus Beauveria bassiana (Balsamo) Vuillemin on Various Agricultural Waste as Growing Media Kurniawan, Irvan; Afandhi, Aminudin; Tarno, Hagus
Research Journal of Life Science Vol 9, No 2 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.02.4

Abstract

Beauveria bassiana is an entomopathogenic fungus that is widely produced for the benefit of biological control agents of various insect pests. The current production that is expected is to use cheap media but produce quality isolates. This study aims to determine colony growth, sporulation and viability of Beauveria bassiana conidia in several agricultural waste media. The experiment was compiled in a complete randomized design and replicated four times. B. bassiana in rice bran media added with cricket flour  had the fastest colony growth while B. bassiana in rice husk media was the lowest, lower than the control treatment. In  wheat bran media added cricket flour has slower colony growth than rice bran with cricket flour media similar to control treatment but has the second highest sporulation after rice bran with cricket flour media. The highest viability belongs to B. bassiana which is grown on rice bran with cricket flour media. The production in each treatment medium has a real effect but the addition of cricket flour has a significant influence in the production of quality isolates.
Association between Predatory Arthropods and Weeds on Sugarcane Plants Devi, Mia Prastika; Rahardjo, Bambang Tri; Tarno, Hagus
Research Journal of Life Science Vol 9, No 3 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.03.4

Abstract

While a large number of farmers in the tropics consider weeds to be just a nuisance, some weeds can potentially have benefits for cultivated crops. Weeds around cultivated plants can act as a biocide, soil improvement, and a food source for humans and animals, as well as a habitat for some insects. Based on the regression analysist, weed density has a relationship to the diversity of predatory arthropod species (R2= 0.026; P<0.001) and the abundance of individual predatory arthropods (R2= 0.010; P<0.001). Beneficial weeds around sugarcane plantations create mutually beneficial interactions. Weeds can be used as a place to live, reproduce, and produce nutrients for predatory arthropods. Research on the role of weeds against natural enemies from predators in sugarcane agroecosystems is also rarely studied. In this study, the focus was on the association of weeds with the diversity and abundance of predatory arthropods in the sugarcane agroecosystem.
Nematicidal Activity of Turmeric Extract against Nematodes Meloidogyne spp. Ulfa, Maria; Himawan, Toto; Tarno, Hagus
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.7

Abstract

In an effort to reduce the impact of synthetic nematicides, a new formula was developed using plant-based nematicides that are more environmentally friendly, one of the basic ingredients that can be used is turmeric. The purpose of this study was to determine the effectiveness of turmeric extract (Curcuma domestica) with various different solvents namely aquades, methanol, and n-hexane at concentration of 5%, 10%, 15% and 20% respectively in inhibiting the activity of Meloidogyne spp. The experiment used was a Completely Randomized Design (CRD). Each treatment was repeated 4 times with 4 controls so that there were 52 experimental units. The result of different research data was analyzed using Analysis of Variance (ANOVA) which was then further tested with Honest Significant Difference (HSD) at the 5% level if real, and t-test for in vivo test. Based on the tested extract, it was found that turmeric extract with various solvent (aquades, methanol, and n-hexane) was effective in inhibiting the activity of Meloidogyne spp. nematodes outside the roots (hatching eggs and penetration), turmeric extract didn’t affect the activity of Meloidogyne spp. in the roots (development and reproduction), vegetable nematicides which the most active in suppressing the population and inhibiting the activity of nematodes was turmeric extract using methanol as a solvent at a concentration of 20%.
Abundance and Diversity of Fruit Flies Species (Diptera: Tephritidae) in Bogor and Depok District Octavia, Evy; Tarno, Hagus; Himawan, Toto; Setiawan, Yogo
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.7

Abstract

Fruit flies (Diptera: Tephritidae) are important pests of horticultural crops. This study investigates the abundance and species richness of Bogor and Depok District as a center area horticulture commodity based on Cue Lure Trap. Each Cue Lure Trap was observed to record the abundance and species richness of fruit flies at seven days intervals from June until August 2021 in Bogor District (Babakan, Mekarsari and Parigi Mekar) and Depok District (Bedahan and Pancoran Mas), West Java, Indonesia. A total of 1,025 individuals were collected and consist of nine species of fruit fly i.e., Bactrocera albistrigata, B. carambolae, B. neocognata, B. verbascifoliae, Dacus conopsoides, D. longicornis, Zeugodacus calumniata, Z. caudatus, and Z. cucurbitae. The number of individuals of fruit flies was highest in the Bedahan, Depok District (F = 5.454, P = 0.002). Zeugodacus cucurbitae was the most abundant species (792 individuals), representing 77.27% of the specimens collected during the sampling period. The highest Shannon–Wiener diversity index of all fruit flies captured in this study was in Bedahan, Depok (1.11) and the lowest in Parigi Mekar, Bogor (0.23). A Bray–Curtis analysis showed that the species compositions of fruit flies on Bedahan and Parigi Mekar had a similarity of 70.1%.
Diversity and Abundance of Insect on Shallot (Allium Ascalonicum L.) with Integrated Pest Management (IPM) and Conventional Patterns Manna, Dorkas Wantiawati; Afandhi, Aminudin; Tarno, Hagus
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.3

Abstract

Research on diversity and abundance of insect on shallot with Integrated Pest Management (IPM) and conventional patterns has been carried out. This study aims to analyze diversity and abundance of insects, damage level of shallot, and relationship between diversity and abundance of insects with growth and production of shallot in IPM and conventional land. This research was conducted in Junrejo village, Batu City in September to December 2017 while identification of insect was carried out at the Laboratory of Plant Pest and Disease, University of Brawijaya. This study uses Yellow pan trap and Pitfall trap methods. The result showed diversity index (H') of IPM land was 2.50 and of conventional land was 2.67, which both are moderate diversity. The abundance of insect on IPM land is higher (8672 individuals) compared to conventional land (3475 individuals). The level of crop damage is lower in IPM land compared to conventional land, while shallot growth and production is higher in IPM land compared to conventional land.
Forecasting Model of Coffee Berry Borer (Hypothenemus Hampei) in Pasuruan District Nadiah, Annisrien; Himawan, Toto; Tarno, Hagus
Research Journal of Life Science Vol 10, No 1 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.01.2

Abstract

Coffee, a globally important commodity, faces difficulties in Indonesia due to pests that reduce its quality and productivity. The coffee berry borer (Hypothenemus hampei) is particularly dangerous, causing hollowed-out coffee seeds and causing massive annual losses worldwide and in Indonesia. Factors such as climate, weather, and other environmental aspects play a pivotal role in influencing pest lifecycles, emphasizing the necessity of understanding the intricate relationship between pests and their environment for effective management. This research aims to contribute to minimizing coffee production losses and harvest failures resulting from H. hampei infestations. This study relies on secondary data from the BBPPTP in Surabaya and NASA. Using panel data regression analysis, the study discovers that coffee berry borer attacks in the Pasuruan District follow a seasonal pattern. Furthermore, with a forecasting accuracy of 15.94%, the model reveals that air humidity, temperature, and previous attacks are the dominant variables influencing current coffee berry borer attacks. The developed model provides a reliable tool for forecasting coffee berry borer attacks, with humidity and previous month's attacks emerging as the most influential factors, particularly in the Pasuruan District of East Java.
Biodiversity and Distribution Rice Arthropods in Malang Indonesia Istiqomah, Rina Nur; Tarno, Hagus; Mudjiono, Gatot
Research Journal of Life Science Vol 10, No 3 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.03.5

Abstract

Climate change is closely related to changes in abiotic conditions. These changes could have an impact on agricultural cultivation and affect the existence of agricultural arthropods. Different environmental conditions will influence the distribution patterns, richness and diversity of arthropods living in cultivated land. The research was conducted at 8 rice cultivation locations in Greater Malang. Observation of arthropods using pitfall and yellow pan traps, with a diagonal pattern. The total arthropods obtained were 3,937 individuals, 39 families, 46 genera and 48 species. The highest predator species found were Micraspis discolor, the herbivore Nilaparvata lugens, other insect is Culista incidens, and the predator is Drynus baegamensis. The highest index analysis for diversity in Lowokwaru H'= 3,075 in the high category, evenness in Karangploso E=0,864 in the high category and dominance in Pagelaran D=0,179 in the low category. PCA analysis of species composition divided into 2 habitat types, lowland and medium land groups. Species richness increased with increasing observation locations. Environmental factors will bring in species that are suitable for the habitat. Further research needs to be carried out regarding ecological variables or environmental factors, which affect the patterns composition and species richness of arthropods.
Use Of Organic Fertilizer and IPM in Certified Organic Rice Farming in East Java Winarno, Dinariningrum Rahma; Tarno, Hagus; Mudjiono, Gatot
Research Journal of Life Science Vol 11, No 1 (2024)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2024.011.01.2

Abstract

This study aims to analyze the effect of organic fertilizer use and Integrated Pest Management (IPM) on the success of certified organic rice farming in East Java. The research method used is descriptive quantitative with Structural Equation Modeling-Partial Least Squares (SEM-PLS) analysis. Data was collected by surveying 100 organic rice farmers in Kediri District. The results showed that the use of organic fertilizers and IPM significantly increased the productivity of organic rice. Organic fertilizers, such as compost and animal manure, can improve soil fertility and crop yields. Meanwhile, IPM helps control pests without using chemical pesticides. The SEM-PLS model confirmed that both variables positively and significantly influence the success of organic rice farming. The results also show that farmers who implement organic farming have a higher level of environmental awareness and are more concerned about consumer health. However, some challenges are still faced, such as limited market access and farmers' lack of knowledge about organic farming techniques. Therefore, more intensive efforts are needed to provide education and training to farmers, as well as to develop policies that support the development of organic farming. In conclusion, the application of organic fertilizer and IPM is the key to the success of organic rice farming in East Java. With the right support, organic farming can be a sustainable solution to increase productivity, preserve the environment, and improve farmers' welfare.
Co-Authors Abdul Latief Abadi Adriani Sunuddin Afiyanti, Mufidah Agus Nugroho, Agus Ainul Hayat Aji Santoso Aji Santoso Akhmad Rizali Alvan Fajarudin Amin Setyo Leksono Aminudin Afandhi Aminudin Afandhi Aminudin Afandhi Andik Isdianto Anggraeni, Alifia Nurma Annike Putri Damayanti Arifin Noor Sugiharto Arin, Iftita Yustitia Arum Yuli Kristanti Bambang Semedi Bambang Tri Rahardjo Bambang Tri Rahardjo Baroro, Ismatul Budi, Setyo DAMAYANTI BUCHORI Dede Durahman Dede Durahman Devi, Mia Prastika Dewi, Citra Satrya Utama Dwi Budi Santoso Dwi Wulandari Edson Begliomini Eko Wahyu Budi Darmawan Eko Wahyu Budi Darmawan Estri Pamungkasih Fadhila Hasna Fauziah, Septia Hana Fery Abdul Choliq Firdausi, Wita Gatot Ciptadi Gatot Mudjiono Gunawan Prayitno Hamdoen, Fadel Muhammad Handoko Handoko Hari Sutrisno Hari Sutrisno Hasan Suprapto Hauroinsiyah, Fatimah Havinda Anggrilika W.S. Herandarudewi, Sekar Mira Hermawan, Romy Hongye Qi Irvan Kurniawan Isna Kartika Wati Istiqomah, Rina Nur Johannis, Midzon Kafif Andani Karisma Aditya Wardani Karuniawan Puji Wicaksono Karuniawan Puji Wicaksono Karuniawan Puji Wicaksono Karuniawan, Anggik Kazuyoshi Futai Liza Afifah Liza Afifah Liza Afifah Luqman Qurata Aini Manna, Dorkas Wantiawati Maria Ulfa Maris Purnanto Maris Purnanto Masahide Kobayashi Maspupah Huzni Maspupah Huzni Meinardhy, Ricky Mintarto Martosudiro Moch. Dawam Maghfoer Mudji Santosa Muhammad Anton Astoni Muhammad Febriansyah Nadiah, Annisrien Nurul Badriyah Octavia, Evy Presti Mardiyani Purwaningtyas Presti Mardiyani Purwaningtyas, Presti Mardiyani Primastya Dinarwika Primastya Dinarwika Rahmadina Fitria Ristanti Ramayanti, Kinta Retno Dyah Puspitarini Ria Febrianasari Ria Febrianasari Rina Rachmawati Rizki Puji Widiastuti Rizki Puji Widiastuti Ronny Pamuji Ronny Pamuji Ryoko Hirata Sari, Riya Fatma Sari, Rose Novita Sari, Sasmita Satoshi Ito Seca Gandaseca Senoaji, Wasis Silvi Ikawati Silvi Ikawati, Silvi Slamet Wahyudi Sofia Ery Rahayu Sri Heriza Sri Heriza Sri Wahyuni Sudiarso Sudiarso Sukandar Sukandar Sulthoni, Fahmi Suputa Suputa Suyono, Tri Syamsulhadi, Mochammad Theresia Rani Kartika Ayu Toto Himawan Toto Himawan Toto Himawan Tsuyoshi Kajisa Wahyudie, Diyan Eko Wardana, Fitri Candra Wasiska Iyati Wiadnya , Dewa Gede Raka Winarno, Dinariningrum Rahma WINDRA PRIAWANDIPUTRA, WINDRA Yahya, Minhajul Qowim Yasa Palaguna Umar Yasushi Mitsuda Yogo Setiawan Zeni Ningrum Zeni Ningrum Zulfaidah Penata Gama