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All Journal Bioscience Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Techno: Jurnal Penelitian BIOMA : Jurnal Biologi Makassar Jurnal Penelitian Pertanian Terapan Jurnal SOLMA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Biosilampari: Jurnal Biologi Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Jurnal Medika Malahayati Floribunda Jurnal Ilmiah Farmasi Farmasyifa Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun Journal of Sustainability Science and Technology JURNAL BIOLOGI PAPUA AMMA : Jurnal Pengabdian Masyarakat
Sri Wahyuningsih
Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Lampung
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Efek Antimitosis Biomutagen dari Tanaman Kembang Sungsang (Gloriosa superba L.) pada Pembelahan Sel Ujung Akar Kecambah Cabai Merah Besar (Capsicum annuum L.) Eti Ernawiati; Sri Wahyuningsih; Yulianty Yulianty
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 2 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.2.214

Abstract

Flame lily (Gloriosa superba L.)  is one of the plants containing colchicine, often used as antimitotic compound, in the whole organ. This study aims to gain the combination of extract source  and extract concentration of G. superba capable of inhibit mitosis in root tip cells of chili  sprouts.  The plant is extracted  using the extraction and dilution methods to determine the extract concentration of the treatment. The squash method is used to make a  mitosis preparation. The study is arranged in a factorial using Randomized Complete Block Design (RCBD) with 4 replications. The first factor is extract source:   roots, stems, and leaves. The second factor is the extract concentration: 0, 20.40, 60, and 80%.   The observation shows that the extract of leaf can inhibit mitosis more than exctract of bulbs and roots.  All of the concentration treatment from all extract sources tend to result in similar mitotic index, but significantly different from cells that are not given treatment. The combination of the source extract and concentration of the extract result in varied mitotic index, but  it is obvious that the leaf extract with a concentration of 80% gives the most inhibiting mitosis index: 1.298%.
ANATOMICAL CHARATERISTICS OF ARACEAE FAMILY IN LIWA BOTANICAL GARDEN, WEST LAMPUNG, LAMPUNG Putri Kendari; Sri Wahyuningsih; Yulianty Yulianty; Martha Lulus Lande
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 7 No. 2 (2020)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v7i2.153

Abstract

Indonesia is one of the countries with high Araceae diversity, which is about  36 genera consisting of 669 species. However research on the characteristics of Araceae in Indonesia is still limited. The existence of Araceae in Liwa Botanical Garden has not been studied. One of the characteristics in assessing plant diversity is anatomical features. Therefore, the aim of this study is to determine the anatomical characteristics of Araceae in Liwa Botanical Garden. This research was conducted from October to November 2019 in Liwa Botanical Garden, West Lampung, Lampung using the cruise method (Cruise Method). Araceae plants that are found identified in the Botany Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung. The anatomical features of the leaves and petioles of Araceae was studied carefully. The results of this study indicate that each type of Araceae plant has different characteristics of epidermal cell structure and stomata. The characteristic shape of the epidermal cells is upright, while the characteristic shape of the stomata is amphibrachyparacitic. A certain types of Araceaeshows s a special characteristic which is having secretory cells.
KAJIAN STRUKTUR ANATOMI DAN MORFOLOGI DAUN PLANLET PISANG KEPOK KUNING HASIL PEMBERIAN EKSTRAK UMBI KEMBANG SUNGSANG SECARA IN VITRO Yoga Aji Saputra; Eti Ernawiati; Rochmah Agustrina; Sri Wahyuningsih
Jurnal Biosilampari : Jurnal Biologi Vol 3 No 2 (2021): Biosilampari
Publisher : LP4MK STKIP PGRI Lubuklinggau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31540/biosilampari.v3i2.1268

Abstract

Plantain is a fruit commodity that has a high level of market demand, but the yield is low. To overcome this problem, tissue culture techniques are used to increase plantlet production in a short time. Plantain have parthenocarpy character, high sterility, and different levels of ploidy. Tissue culture with the addition of flame lily tuber biomutagen containing colchicine was used as a strategy to overcome the above constraints because it was thought to increase the production of polyploid banana plantlets. Changes in the anatomical and morphological structures are indicators of polyploidy. The purpose of this study was to obtain information on changes in the anatomical and morphological structures of plantain leaves as a result of giving in vitro plantlet propagation media as an indicator of the formation of polyploidy. Plantlets were grown on tissue culture media supplemented with three treatments: 10% flame lily tuber extract, 0.1% pure colchicine, and without addition (control). The parameters observed were epidermal cell size, stomata size, stomata index, number and leaf area. Epidermal cell size, stomata size and stomatal index were analyzed using range values ​​and mean values. The number of leaves, and the average wide of leaf, is then shown in the form of a bar chart. The results showed that administration of flame lily tuber extract was able to increase epidermal cell size, stomata size, wide of a leaf, and able to reduce the stomata index and number of leaves.
Variation of response induced by ceratorhiza on Dendrobium discolor and Phalaenopsis amabilis to odontoglossum ringspot virus infection based on disease intensity and plant resistance level Mahfut Mahfut; Mitha valentina treesya Panjaitan; Tundjung Tripeni Handayani; Sri Wahyuningsih
Bioscience Vol 6, No 1 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202261114027-0-00

Abstract

The popular cultivated orchid is Phalaenopsis sp. and Dendrobium sp. Mycorrhizae are very important for orchid plants to complete their life cycle. Mycorrhizae are capable of symbiosis with the root system of the host plant which can increase the absorption of these elements. The purpose of this study was to find out the symptoms of infection, the intensity of infectious diseases, the level of resistance to infection. The method used in this study was completely randomized design (CRD) with 2 factors and 4 iterations, the first factor was the type of orchid used Phalaenopsis amabilis (A1) and Dendrobium discolor (A2), and the second factor was the type of administration of mycorrhizal., Virus , and mycorrhizal virus. Observation of the symptoms of the disease is done by looking at the symptoms that appear on the leaves that have been infected with ORSV, while to determine the intensity of the disease and the level of resistance can use a predetermined formula. The results showed that the disease intensity in Phalaenopsis amabilis was more severe than in Dendrobium discolor, which was 40% on each leaf. The level of resistance in Dendrobium discolor is more resistant than Phalaenopsis amabilis, this indicates that Dendrobium discolor on average has a tolerant response. Ceratorhiza could not suppress the intensity of the disease, because from the results obtained, treatment of orchids inoculated with mycorrhiza and virus showed more severe symptoms than treatment only inoculated with virus. Ceratorhiza was unable to increase the resistance of Phalaenopsis amabilis and Dendrobium discolor, this was because treatment of orchids inoculated with mycorrhiza and virus showed a very simple response compared to treatmentonly inoculated with virus.Anggrek populer dibudidayakan adalah Phalaenopsis sp. dan Dendrobium sp. Mikoriza sangat penting bagi tanaman anggrek untuk melengkapi siklus hidupnya. Mikoriza  mampu bersimbiosis dengan sistem akar tanaman inang yang dapat meningkatkan daya serap unsur tersebut. Tujuan penelitian untuk mengetahui gejala infeksi, intensitas penyakit menular, tingkat resistensi terhadap infeksi. Metode penelitian: Rancangan Acak Lengkap (RAL) dengan 2 faktor dan 4 iterasi, faktor pertama adalah jenis anggrek yang digunakan Phalaenopsis amabilis (A1) dan Dendrobium discolor (A2), dan faktor kedua adalah jenis pemberian mikoriza, virus, dan virus mikoriza. Pengamatan gejala penyakit dilakukan dengan melihat gejala yang muncul pada daun yang telah terinfeksi ORSV. Hasil penelitian menunjukkan intensitas penyakit pada Phalaenopsis amabilis lebih parah dibandingkan dengan Dendrobium discolor yaitu 40% pada setiap daun. Tingkat ketahanan pada Dendrobium discolor lebih tahan dibandingkan Phalaenopsis amabilis. Dendrobium discolor memiliki respon yang toleran. Ceratorhiza tidak dapat menekan intensitas penyakit. Pengobatan  anggrek yang diinokulasi dengan mikoriza dan virus menunjukkan gejala yang lebih parah daripada pengobatan yang hanya diinokulasi virus. Ceratorhiza tidak mampu meningkatkan ketahanan terhadap Phalaenopsis amabilis dan Dendrobium discolor, hal ini dikarenakan perlakuan anggrek yang diinokulasi mikoriza dan virus menunjukkan respon yang sangat sederhana dibandingkan dengan perlakuan yang hanya diinokulasi virus.
Study of Resistance on Orchids Induced Rhizoctonia to ORSV Based on Secondary Metabolite Analysis Mahfut Mahfut; Widi Aryani; Tundjung Tripeni Handayani; Sri Wahyuningsih
TECHNO: JURNAL PENELITIAN Vol 11, No 2 (2022): TECHNO JURNAL PENELITIAN
Publisher : Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/tjp.v11i2.3044

Abstract

Dendrobium discolor and Phalaenopsis amabilis are in high demand because of their beautiful shape, color, texture, and flower arrangement. The current obstacle to orchid cultivation is infection with Odontoglossum ringspot virus (ORSV). One of the efficient ways to control the infection of this pathogen and does not cause an impact on the environment is to use Rhizoctonia mycorrhizae. Mycorrhizal relationship with plants will form induced resistance. Plants resulting from induced resistance will induce secondary metabolite, namely peroxidase enzymes. This type of secondary metabolites will prevent the growth and development of pathogens and plants become resistant to pathogenic infections. The purpose of this study was to determine differences in peroxidase enzyme activity and differences in resistance to P. amabilis and D. discolor after Rhizoctonia induction against ORSV infection. This research was carried out in several stages, namely plantlet preparation, mycorrhizal inoculation, mycorrhizal inoculation, and peroxidase enzyme activity analysis using a UV Vis spectrometer at a wavelength of 420 nm. The results showed that D. discolor had a higher peroxidase enzyme activity than P. amabilis. In the treatment application factor, the peroxidase enzyme activity was the highest in the Mycorrhizal Virus treatment compared to the Mycorrhizal and Virus treatments. This study showed that D. discolor was more resistant to ORSV infection than P. amabilis. and the application of Mycorrhizae proved to be successful in increasing the activity of the peroxidase enzyme as a response to plant protection against viral infections.
Variation of resistance response of orchids induced by Rhizoctonia against the infection of Odontoglossum ringspot virus (ORSV) based on percentage of disease development Fania Nur Izzati; Mahfut Mahfut; Eti Ernawiati; Sri Wahyuningsih
Journal of Sustainability Science and Technology Vol. 1 No. 1 (2021)
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/josst.v1i1.3

Abstract

Orchid is one of the largest groups of flowering plants that are in great demand by society. Besides, the various flowers with a long vase life, the price of orchids is also stable and affordable. Phalaenopsis and Dendrobium are the most popular types of orchids. To anticipate this, orchid cultivation needs to be improved. However, orchid cultivation is not always accompanied by the effort of disease prevention, particularly those caused by viruses. The virus that most infects orchids is the Odontoglossum ringspot virus (ORSV). Symptoms that appear on the leaves are chlorosis, mosaic with a pattern of lines, circles, and necrotic rings/rings. Control of viral infections can utilize organisms that are able to induce resistance, namely mycorrhizae. Mycorrhizae will penetrate into the root cortex tissue and provide nutrients for the orchid. Mycorrhizae that are quite often found are Rhizoctonia and are applied by induction to orchids. Induction was undertaken by planting orchids in Rhizoctonia inoculum until anatomically a peloton structure (solid coils) was formed on the orchid roots. The aim of this study was to study the response of orchid plants in the form of percentage incidence and intensity of the disease and the level of plant resistance to ORSV infection in Phalaenopsis amabilis and Dendrobium discolour which had been induced by Rhizoctonia. This research was conducted at the Laboratory of Botany, Biology, FMIPA, Universitas Lampung, using a factorial completely randomized design (CRD). Factor 1 was the type of orchid and factor 2 was mycorrhizal induction, virus inoculation, and a combination of both with 4 replications. The finding indicated that Phalaenopsis amabilis was more susceptible to ORSV infection than Dendrobium discolour based on a higher incidence and intensity of the disease. Mycorrhizal induction and virus inoculation (MAV) in Dendrobium discolour showed an increase of resistance response compared to Phalaenopsis amabilis.
Produksi Cabai Merah (Capsicum annuum L.) dari Benih yang Diinduksi Medan Magnet 0,2 mT dan Diinfeksi Jamur Fusarium sp. Rochmah Agustrina; Herta M. Manullang; Bambang Irawan; Sri Wahyuningsih; Sumardi Sumardi
Jurnal Biologi Papua Vol 12, No 1 (2020)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.287 KB) | DOI: 10.31957/jbp.1063

Abstract

As one of the horticultural plants that has important economic value, the cultivation of red chili (Capsicum annuum L.) is often constrained by the attack of Fusarium sp., which can cause losses due to decreased production. Induction of the magnetic field on the plant is known to be able to increase growth, development, and production in various plants.  In this research, we studied the production of chili grown from seeds exposed to a 0.2 mT magnetic field and then infected with Fusarium sp. The study was conducted factorial using a completely randomized design with 2 factors. The first factor is the exposure of a 0.2 mT magnetic field to the seeds consisting of controls, without exposure to a magnetic field (M0), magnetic field exposure for 7 minutes 48 seconds (M7), and 15 minutes 30 seconds (M15). The second factor is Fusarium sp. infection on seeds that have been exposed to a magnetic field for 60 minutes by immersion of the seeds with spore suspension of Fusarium sp. with the density of 1 x 107 conidia/ml (F60), and without Fusarium sp. infection (F0). Each treatment unit was repeated 5 times. The data obtained were analyzed for variance at α = 5% followed by the least significant difference test using the Fishers Test at α = 5%. The results of data analysis showed that the interaction of 0,2 mT magnetic field exposure and infection of Fusarium sp. in chili seeds significantly affect the stomata index, the fruiting rate, and the number of fruit but do not affect the content of total chlorophyll, carbohydrate, as well as the number of flowers. The highest fruiting rate and number of fruits obtained from the treatment result of 0.2 mT magnetic field exposure for 15 minutes 36 seconds without Fusarium sp. infection (M15F0). Infection of Fusarium sp. in these seeds (M15F60) reduce the fruiting rate and the number of fruit but it is not significant and even almost similar with the fruiting rate and the fruit number of the plant growth from control treatment (M0F60).   Key words: magnetic field, Fusariumsp., seed, and production.  
Penyuluhan Teknik Perbanyakan Tanaman Hias Sebagai Penunjang Perekonomian Keluarga Eti Ernawiati; Tundjung Tripeni Handayani; Sri Wahyuningsih; Mahfut Mahfut
Jurnal Pengabdian Kepada Masyarakat (JPKM) TABIKPUN Vol. 4 No. 2 (2023)
Publisher : Faculty of Mathematics and Natural Sciences - Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpkmt.v4i2.113

Abstract

Pemberdayaan ekonomi keluarga merupakan bentuk kepedulian atas permasalahan kemiskinan dan penciptaan lapangan kerja. Ibu-ibu rumah tangga di pedesaan perlu didorong berwirausaha agar dapat menunjang ekonomi keluarga. Wirausaha budidaya tanaman hias cocok bagi ibu-ibu rumah tangga karena berskala rumahan dengan modal kecil. Perbanyakan tanaman merupakan salah satu komponen penting dalam budidaya tanaman hias untuk menjamin ketersediaan bibit berkualitas. Ibu-ibu rumah tangga di Desa Bandar Sari, Kecamatan Padang Ratu, Kabupaten Lampung Tengah sebagian besar berpendidikan rendah sehingga pengetahuan dan keterampilan budidaya tanaman hias mereka terbatas. Oleh karena itu kegiatan ini bertujuan untuk memberikan pengetahuan dan keterampilan teknik perbanyakan tanaman hias. Kegiatan dilaksanakan menggunakan metode ceramah dan demonstrasi. Hasil evaluasi kegiatan yang dilakukan melalui pretest dan posttest menunjukkan peningkatan pemahaman peserta yang cukup signifikan sebesar 76,96 %, yaitu dari nilai rata-rata pretest 48,84 meningkat menjadi 63,46 pada posttest. Sesi diskusi juga berlangsung kondusif dengan pertanyaan-pertanyaan peserta yang menunjukkan keingintahuan yang tinggi untuk memperoleh pengetahuan.
KEANEKARAGAMAN MARGA Nothaphoebe Blume, SUKU LAURACEAE (MEDANG - MEDANGAN) DARI PROVINSI LAMPUNG, KOLEKSI HERBARIUM BOGORIENSE (BO) Fersiana Riska Devilia; Yulianty -; Sri Wahyuningsih; Deby Arifiani
Floribunda Vol. 7 No. 3 (2023): Floribunda Oktober 2023
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32556/floribunda.v7i3.2023.431

Abstract

Nothaphoebe Blume is a genus of the Lauraceae or Medang-medangan family, and is included in the Persea subgroup together with the genera Alseodaphne, Dehaasia, Persea, and Phoebe. Information on the diversity of Nothaphoebe in Sumatra, especially in Lampung province, has never been reported. Therefore, the aim of this study was to understand the morphological characters and species of Nothaphoebe occured in Lampung. The morphological characters observed included twigs, leaf shape, flowers, and fruits. The results of morphological characterisation show that there are five species of Nothaphoebe, i.e., Nothaphoebe falcata, Nothaphoebe foetida, Nothaphoebe macrocarpa, Nothaphoebe magnifica, and Nothaphoebe umbelliflora. Identification key for species, descriptions of each species and distribution maps are presented in this article. Keywords: Herbarium Bogoriense, Lauraceae, Morphology, Nothaphoebe, Lampung Province
Implementasi Aplikasi Sistem “Pakar Anggrek” Berbasis Android Di Kebun Raya Liwa Mahfut; Admi Syarif; Tundjung Tripeni Handayani; Sri Wahyuningsih; Kurnia Muludi; Muhammad Irfan Ardiansyah
AMMA : Jurnal Pengabdian Masyarakat Vol. 2 No. 7 : Agustus (2023): AMMA : Jurnal Pengabdian Masyarakat
Publisher : CV. Multi Kreasi Media

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Natural orchids are one of the original flora collections in Liwa Botanical Gardens that need to be preserved. Based on the results of our previous research, it is known that some natural orchids show symptoms of disease infection. The Liwa Botanical Gardens do not yet know a safe disease infection control technique. Disease control is still using pesticides that are harmful to the environment. The results of our previous research have resulted in new methods and solutions for disease control through a web-based expert system. This method was considered very helpful for diagnosis but was considered inefficient, so the Android-based "Orchid Expert" application was developed. This Community Service Dissemination of Research Results (PKM-DHR) activity is a continuation of our previous research results. The problem-solving formulation method is carried out through direct counselling and training at the Liwa Botanical Gardens. The activity was carried out on Wednesday, August 16, 2023, with a total of 30 participants. The results of the training activities at the demonstration stage of downloading and using the application show that the accuracy of the system diagnostic results is valid, with an accuracy rate of 92.9%. This can be interpreted as meaning that this application can be applied in society. The results of this activity indicate that the Liwa Botanical Garden management understands and is able to apply the development of natural orchid disease control methods as a conservation effort. Furthermore, the Liwa Botanical Garden is also expected to be able to independently diagnose and control natural orchid disease infections.
Co-Authors Achmad Arifiyanto Admi Syarif Agustrina, Rochmah Aryani Refiliya Bambang Irawan Deby Arifiani Emantis Rosa Endang Nurcahyani Eti Ernawiati Eti Ernawiati Eti Ernawiati Eti Ernawiati Fania Nur Izzati Fania Nur Izzati Fersiana Riska Devilia Garri Abdi Nusantara Hardoko Insan Qudus Hasti Maulidya Fassya Herta M. Manullang Istiqomah istiqomah Kinasih Cahyono Kurnia Muludi Mahfut Martha Lulus Lande Martha Lulus Lande Martha Lulus Lande Mitha Valentina Treesya Panjaitan Muhammad Irfan Ardiansyah Putri Kendari Ratri Mauluti Larasati Sahira Josy Arifannisa Sukimin Sukimin Sumardi, Sumardi Sutyarso Tundjung T. Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Valentina Dwi Anggita Sari Wanda Amelia Widi Aryani Yoga Aji Saputra Yulianty Yulianty - Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Zulkifli Zulkifli Zulkifli Zulkifli