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The Use of Fish Flour and Palm Sugar Medium to Grow Mataram Indigenous Isolate of Entomopathogenic Bacillus thuringiensis for Controlling Aedes agypti Larvae Suryadi, Bambang Fajar; Hidayat, Hilal Aisyah; Tresnani, Galuh
Journal of Tropical Life Science Vol 9, No 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.446 KB) | DOI: 10.11594/jtls.09.02.04

Abstract

To grow Mataram indigenous local isolate of entomopathonic Bacillus thuringiensis using 4 combinations of fish flour and palm sugar natural liquid medium. The culture was then tested against 3rd instar Aedes aegypti larvae to gain lethal concentration (LC) value within 72-hour test. Four combinations of fish flour and palm sugar liquid medium (in 1:1; 1:3; 1:5 and 1:7 weight ratio) were made to grow B. thuringiensis isolate Bt-TP2B. Cell, endospore and protein crystal were recorded every 24 hours within 72-hour incubation. Seventy-two-hour-old B. thuringiensis cultures from those media were serially diluted, then tested against 3rd instar B. thuringiensis larvae. Larval death was recorded every 24 hours within 72-hour observation to obtain lethal concentration (LC) values. Results from all medium were compared to those of NYSM standar medium. B. thuringiensis isolate BT-TP2B could grow in fish flour and palm sugar natural medium. The highest toxicity (the lowest LC) was seen on B. thuringiensis grown on fish flour and palm sugar natural medium in 1:1 weight ratio. The LC50 values were 1.98x103 cell/mL (in 24-hour observation) and 1.49x103 cell/mL (in 48-hour and 72-hour observation), while LC90 values were 1.35x103 cell/mL in 24-hour observation and 1.58x103 cell/mL in 48 and 72-hour observations). These values were lower (more toxic) compared to those of NYSM standard medium. Fish flour and palm sugar natural medium could be used to grow entomopathogenic B. thuringiensis. Moreover, this natural medium could increase toxicity of B. thuringiensis against B. thuringiensis3rd instar larvae. 
Population Density and Habitat Preferences of the Rinjani Scops Owl (Otus jolandae) in the Mandalika Special Economic Zone, Lombok, Indonesia: Population density and habitat preference of Otus jolandae Permatasari, Baiq Dewi; Suana, I Wayan; Hadiprayitno, Gito; Tresnani, Galuh
Journal of Tropical Life Science Vol. 15 No. 2 (2025): In Press
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/e0jyvz11

Abstract

The Rinjani Scops Owl (Otus jolandae) is an endemic species of Lombok, closely associated with Mount Rinjani National Park. In 2023, its range was newly recorded in the Mandalika Special Economic Zone (SEZ), extending its known distribution. Understanding the population density and habitat preferences of this bird is crucial for effective management and conservation. From April to June 2024, a study was conducted in Mandalika SEZ, covering an area of 1,175 hectares. The point count method was used to record individuals based on sightings and calls. Vegetation analysis was performed at observation sites to identify preferred tree species and calculate habitat preferences using the selection index. Twenty-three individuals of O. jolandae were observed across seven sites, with a population density of 0.02 individuals per hectare—lower than densities recorded in Mount Rinjani National Park—which reflects the significant ecological pressures from development activities. The habitat includes 21 tree species, but the owl actively uses only 14 for activities such as perching, roosting, and nesting. Samanea saman had the highest preference index (3.424), making it the most favored tree, followed by Gmelina arborea and Nauclea orientalis. The species relies heavily on large-diameter trees and the middle canopy layer, reflecting its need for stable and protective habitats. This study provides essential data for future conservation programs and highlights the Mandalika SEZ’s potential as a birdwatching ecotourism destination featuring this unique endemic species