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Journal : Biocelebes

Studi Efektivitas Ekstrak Daun Sereh Wangi (Cymbopogon nardus L.) Sebagai Anti Fungi Candida albicans Eka Fitriani; Muhammad Alwi; Umrah Umrah
Biocelebes Vol. 7 No. 2 (2013)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The study was held on effectiveness of extract lemongrass leaf (Cymbopogon nardus L.) as a anti septic of Candida albicans. The study conducted as long as 3 months, from march to may 2013. Extracts was obtained by maceration method. This study aims to determine the extract can be prevent the growing of the Candida albicans, and to determine the effective concentration to prevent the growing of the fungus Candida albicans. This study is an experimental research with a completely randomized design. Bes trew diffusion method by a concentration of 25%, 50%, 75%, 100% and metronidazol liquid is used to determine the zone diameter inhibition in the five treatment and five replications. Inhibition zone diameter data were analyzed statistically ANOVA and Duncen Multiple Range Test (DMRT). The concentration stratified of extract effect on the zone diameter growth of Candida albicans. Based on the study, concentration at 75% is the best to be inhibited the growth of Candida albicans in conclusion, the extract is anti septic to the growth of Candida albicans. Keywords: Cymbopogon nardus L., Maceration, Candida albicans
Analisis Mikrobiologi Stik Kentang Goreng di Cafe Lesehan Talise Palu Rizki Purnamasari; Umrah Umrah; Muhammad Alwi
Biocelebes Vol. 7 No. 2 (2013)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Research entitling "Microbiological Analysis of Stik Potato Fry in Cafe Lesehan Talise Palu" have been executed in March until May 2013 as a mean to analyse the cemaran microbe of at stik potato fry in Cafe Lesehan Talise Palu and to know what stik potato fry the have fulfilled the Health Standard specified by BPOM by mikrobiologis. Result of research indicate that pursuant to microbiological analysis quantitatively is ALT And MPN of at sampel stik potato fry in Cafe Lesehan Talise Palu show 2 sampel which is not growed by colony, 6 sampel which is < 3,0 x 104 CFU/g, 1 incalculable sampel and 1 sampel with the result 3,1 x 104 CFU/g for the ALT of bacterium, while for the ALT of kapang obtained by 1 sampel which is not growed by the colony and 9 sampel which is < 3,0 x 104 CFU/g, and also for the ALT of khamir obtained by 3 sampel which is not growed by the colony, 6 sampel which is < 3,0 x 104 CFU/g and 1 sampel with the result 3,7 x 104 CFU/g. Most Probable Number (MPN) show 9 sampel which is 3,0 x 100 MPN/g (<3) and 1 sampel with the result 2,3 x 101 MPN/g namely Cafe C. From examination result 10 sampel stik potato fry knowable that 90% still fulfill the Health Standard specified by Director General BPOM of while 10% do not fulfill the Health Standard specified by Director General BPOM. Keywords: Microbiological Analysis, Stik Potato Fry, ALT and MPN
Deteksi Bakteri Coliform Dan Escherichia coli Pada Depot Air Minum Isi Ulang Di Kota Pasangkayu Kabupaten Mamuju Utara Sulawesi Barat Hasriani Hasriani; Muhammad Alwi; Umrah Umrah
Biocelebes Vol. 7 No. 2 (2013)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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This research entitle "Detect the bacterium of Coliform and Escherchia coli at depot of drinking water refill the diKota Pasangkayu of Sub-Province of Mamuju of North of West Sulawesi" have been executed in March until May 2013, in Biological Laboratory of Biological Majors Base of Faculty MIPA Tadulako University Palu. Target of this research to detect the bacterium of Coliform and Escherchia coli at depot of drinking water refill in Town of Pasangkayu of Sub-Province Mamuju North West Sulawesi and also determine the quality by mikrobiologis pursuant to Standard of Indonesia National (SNI). Method used in this research is Most Probable Number (MPN). Result of Research indicate that at fifth of depot which have in test, that is at test of MPN Coliform of there is three depot which are positive namely depot B for the BI OF standard water with the value 4 MPN/100 mL sampel, for the depot of C namely standard CI water with the value 150 MPN/100 mL sampel, CII irrigate the process by niali 23 MPN/100 mL sampel, CIII irrigate the gallon with the value MPN 21 MPN/100 mL sampel, while for the depot of D namely IN standard water show the value MPN 43 MPN/100 mL sampel. Pursuant to examination of Coliform Fecal and Escherchia coli expressed by all sampel is negativity. Keywords: Coliform, Escherchia Coli, and Drinking water refill.
Deteksi Bakteri Coliform DAN Escherichia coli Pada Minuman Es Jeruk Di Cafe Lesehan Pantai Talise Palu Ikha Wahyuni; Muhammad Alwi; Umrah Umrah
Biocelebes Vol. 7 No. 2 (2013)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Research entitle "Detect the Bacterium of Coliform and Escherichiacoli at Ices Beverage of Orange In Coastal Cafe Lesehan of Talise Palu" have been executed as a mean to detect and know the amount of bacterium Coiform and Escherichiacoli at ices beverage of orange in Coastal Cafe Lesehan of Talise Palu. This research have the character of descriptive, by using method of Standard Plate Count (SPC) And Most Probeble Number (MPN). Pursuant to research result from test SPC indicate that 50 % impure Cafe Lesehan because standard ineligibility of quality which is in specifying BPOM that is Cafe A of counted 1,1 x 104 CFU/ml, Cafe C of counted 4,3 x 104 CFU/ml, Cafe D of counted 1,5 x 104 CFU/ml, Cafe H of counted 1,8 x 104 CFU/ml. Examination of Method MPN from there are 30 % impure Cafe Lesehan by bekteri Coliform at ices beverage orange of because standard ineligibility of quality specified by BPOM that is at Cafe A of counted 143 x 101 MPN/ml, Cafe H of counted 2,33 x 101 MPN/ml, and Cafe J of counted 1,23 x 101 MPN/ml. Pursuant to examination of coliform fecal and test the Escherichia coli expressed by all sampel is negativity. Keywords: Coliform and Escherichia coli, ices beverage of orange, Cafe Lesehan.
POTENSI LIMBAH CAIR TEMPE SECARA MIKROBIOLOGIS SEBAGAI ALTERNATIF PENGHASIL BIOGAS Nur Hikma; Muhammad Alwi; Umrah Umrah
Biocelebes Vol. 8 No. 1 (2014)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The research was about “Potential of Liquid Waste of Tempe Microbiologically as the alternative of Biogas Producer” had been conducted from July to September 2013 with the purpose of finding out of liquid waste of tempe microbiologically as the alternative of biogas producer and finding out the best concentration in media of liquid waste of tempe producer biogas and finding the best time for fermentation in producing biogas. The research method used is experimental which designed in the form of Complete Random Design (RAL), comprising 6 treatments and 3 times repetition. The research result shows that all treatment can produce biogas. The highest biogas volume is the treatment P5 (50% : 50%) which was as 128.10cm3. The best fermentation time occured on the third day (72 hours) up to the fourth day (96 hours) of fermentation. Keywords: Liquid Waste of Tempe, Microbiology and Biogas.
PEMANFAATAN LIMBAH CAIR TEMPE DAN LIMBAH CAIR TAHU DENGAN MENGGUNAKAN INOKULUM YANG BERBEDA UNTUK PRODUKSI BIOGAS Niluh Budi Artini; Muhammad Alwi; Umrah Umrah
Biocelebes Vol. 8 No. 1 (2014)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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This research was about “ The Usage of Liquid Waste of Tempe and Liquid Waste of Tofu by Using Different Inoculum to Produce Biogas”. The objective of the research was to use liquid waste of tempe and tofu by using different inoculum in producing biogas, finding out the interaction between the waste and microba inoculum in producing biogas, finding out the biggest amount of gas produced by liquid waste of tempe and tofu by using different inoculum in producing biogas and finding out the best time for fermentation in producing biogas. This research was designed by using Factorial Pattern of Complete Random Design (RAL) 2x2 comprising 4 treatments and 3 times repetition. The treatment examined was P1 )Liquid Waste of tempe + Rumen), P2 (Liquid Waste of tofu + Rumen), P3 (Liquid Waste of tempe + Rumen Contents), P4 (Liquid Waste of tofu + cow feces ) with the comparison of every treatment 50% :50%. Based on the research result, it shows that every treatment examined can produce biogas. The highest volume of examined treatment is P1 treatment (Liquid Waste of tempe + Rumen Contents) which was 57.15cm3. The best time for fermentation was the third day (72 hours). Keywords: Liquid Waste of tempe, Liquid Waste of tofu, Inoculum and Biogas.
KARAKTERISASI LAJU PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus) PADA MEDIA DEDAK PADI (Oryza sativaL) DAN JAGUNG (Zea mays L) Nova Damayanti; Umrah Umrah
Biocelebes Vol. 8 No. 2 (2014)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The aim of this study is to characterize the growth rate of mycelium of oyster mushroom (Pleurotus ostreatus). It’s expected that the out come of this study is to find out the best medium which suitable for oyster mushroom propagation. The experiment was designedbased oncompletely randomized design(CRD) consistingof 6 treatmentsand 3 repetitions. The treatment were various ratio of rice bran and corn on the growth medium. The ratio used as treatment (rice : corn) were 100:0 (P1), 80:20 (P2), 60:40 (P3), 40:60 (P4),80:20 (P5), 0:100 (P6). Result of experiment showed that medium P3 was the bestin supporting the growth rate of mycelia which in average of 8,45 mm/day. It were higher than it in other media P4 (7,95 mm/ day), P6 (7,45 mm/ day), P5 (7,08 mm/ day), P1 (6,33 mm/ day) and P2 (5,5 mm/ day). Mycelia on this medium was growth faster that mycelium on the other medium. Keywords: Growthrate,Oyster Mushroom(Pleurotus ostreatus), ricebran (Oryza sativaL), corn (Zea maysL)
STUDI LAJU PERKEMBANGAN BUAH KAKAO PASCA APLIKASI MEDIA JAMUR TIRAM (Pleurotus ostreatus (Jacq) P. Kumm) Roliana Roliana; Umrah Umrah; Miswan Miswan
Biocelebes Vol. 9 No. 2 (2015)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The study Cocoa Fruit rate Post Application Development Media Media Mushroom Compost Waste Oyster Mushroom (Pleurotusostreatus (Jacq) P. Kumm) This study was conducted in the village Langaleso, in order to determine the effect of dose and post-application of compost media oyster mushroom (Pleurotusostreatus( Jacq) P. Kumm) on the rate of development of the fruit and seed weight of cocoa. The research was designed in completely randomized design (CRD), consisting of seven treatments and three replications. The composition of the treatment of waste compost fertilizer applications on any media oyster mushroom cocoa plant P (control, without fertilizer application), P1 (application of compost 500 g), P2 (application of compost in 1000 g), P3 (compost application 1500g), P4 (compost application 2000 g), P5 (compost application 2500 g), P6 (3000 g of cocoa compost applications). Parameter observations pace of development of the cocoa plant fruit and cocoa bean weight. The rate of development of cocoa pods showed between fruit length and circumference of fruit on a day to Fifty. Results of the study showed weight cocoa beans treated (P5) is the best treatment in terms of the average weight of the cocoa beans 45.81 g.Keywords : Cocoa Beans, Compost Waste Oyster Mushroom Media, (Pleurotus ostreatus (Jacq) P.Kumm)
PENGARUH PEMBERIAN BIOKOMPOS (BAHAN AKTIF Trichoderma sp., FORMULA SEDIAAN TABLET) TERHADAP PERTUMBUHAN BIBIT KAKAO (Theobroma cacao L.) Umrah Umrah; Firji Della Sugeha; Miswan Miswan
Biocelebes Vol. 9 No. 2 (2015)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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This research were performed to find out the effect of bio-compost treatment (Trichoderma sp. active material, formula of tablet-form) towards the cacao seedling’s growth.This research has been conducted between June-August 2014, at Langaleso Village, Kecamatan Dolo, districtof Sigi, Provinsi of Central Sulawesi. This research was designed in form of six treatments and threereplication according to Completely Randomized Design.The design of treatments is mixture of soil and bio-compost Trichoderma sp. active material formula of tablet-form, which P0 (soil without bio-compost as control), P1 (mixture of soil and bio-compost with 5 tablets per kg substrate Trichoderma sp. active material), P2 (10 tablets per kg substrate), P3 (15 tablets per kg substrate), P4 (20 tablets per kg substrate), and P5 (25 tabletsper kg substrate). The Parameter observed includes plant hight (cm) and number leafs (sheet) that observed in each week during six weeks, biomass plant dryweight (g) that observed in sixth week. The result of reseach showed that bio-compost treatment (Trichoderma sp. Active material formula tablet-form) indicate significant effect to cacao seedling. The conclusion is P5 treatment are the best among the other treatments including control, according to the growth rate wereon plant height (22,40 cm), number of leafs (9,67) and biomass plant dryweight (2,12 g).Keywords : cacao seedling, bio-compost, Trichoderma sp.
PENGARUH PEMBERIAN LIMBAH MEDIA JAMUR TIRAM (Pleurotus ostreatus (Jacq)P.Kumm) TERHADAP PERTUMBUHAN BIBIT KAKAO (Theobroma cacao L.) Setia Ayu Lestari; Umrah Umrah; Miswan Miswan
Biocelebes Vol. 10 No. 2 (2016)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The  growth of cocoa plant seedlings (Theobroma cacao L.) can be Increased by using organic material, for examples oyster mushroom media waste (Pleurotus ostreatus (Jacq)P.Kumm). The purpose of this study was to determine the effect of oyster mushroom media waste on the growth of cocoa plant seedlings. This study was conducted during period of March until May 2015. Cocoa plant seeds was planted in plastic bags with size 30 x 40 cm, designed in a Completely Randomized Design (CRD). The treatment consists of P0 (soil media without oyster mushroom media waste, as a negative control);P1 (soil media is 90% + oyster mushroom media waste is 10%), P2 (soil media is 80%+ oyster mushroom media waste 20%), P3 (soil media is 70% + oyster mushroom media waste 30%), P4 (soil media is 60% + oyster mushroom media waste is 40%), P5 (soil media is 50% + oyster mushroom media waste 50%), P6 (soil media is 3 kg + NPK Fertilizer is 3 g, as a positive control).The observation parameters includes the plants high, number of leaves, stem diameters, plants fresh biomass and plants dry biomass. The results was showed that the best tretment is P2 acording there are average parameters,  include 24,63 cm high of plant; 10,66 number of leaves; 0,42 cm stem diameters; 4,73 grams of fresh biomass and 2,15 grams of dry biomass.                      Keywords : Cocoaseedlings, oyster mushroom media waste (Pleurotus ostreatus (Jacq)  P.Kumm), Theobroma cacao L.
Co-Authors A. St. Latifah Humairah A AA Sudharmawan, AA Adhiwijaya, Ardian Adi Putra, Andi Budiyanto Aisah Jamili Alwi Alwi Alwi Millang Amalia Purnamasari Zainal Amiruddin Kasim Andi Lindhemuthianingrum Annawaty Annawaty, Annawaty Asfiani, Asfiani Asri Ismail Asri Pirade Paserang Asriani Hasanuddin Asrul Asrul Asrul Asrul Azzahra, Andini Shafa Arezy Azzahra Bidayanti Ruru Budi Indarsih, Budi Burhan, Kurnia Dana Sulistia Dewi, Ni Made Aryani Saraswati Dhini Arwindah Dian Sri Anugrah Djoko Kisworo Eka Fitriani Eny Yuniati Evisilvia Evisilvia Fahri Fahri Febiyanti Firdasari Firdasari Firji Della Sugeha Fitri Kurnianingsih Fitriani Fitriani Fitriani Husain Gina, Fina Alimatul Hardianti Lestari Hamid Hasriani Hasriani Humaero, Siti I Nengah Suwastika I Nyoman P. Aryantha Ika Merdeka Wati Ikha Wahyuni Ilham Bakri Abdul Rahim Thaha,Isrun, Ilham Bakri Irawan, Adit IRWAN LAKANI Jamaluddin Jamaluddin Jaya, I Nyoman Sukartha Jesica Pasaribu Juliana Juliana Kasman Sulaiman Khadijah Kirana Kirana Laksono Trisnantoro Lamadjido, Sri Rahayu Latifah Merdekawaty Lina Mahardiana Makmur, Baitul Mardiana Upara Maulana, Ardian Maulida, Nurhidayatul Meryany Ananda Miranty Sirappa Miswan Miswan Moh. Iqbal, Moh. Mufida Al Idrus Muh. Arsal N. Maddo Muhaemin Muhaemin Muhammad Alwi Muhammad Amiruddin Muhammad Saleh Muhammad Yusril Basir Murnawati Murnawati Musdalifa Musdalifa Musjaya M. Guli Mustafid Rasyiid Mutma Inna Mutmainah Mutmainah Mutmainah Mutmainah Mutmainah Mutmainah Naksabandi, Roydatul Robbi Ni Ketut Dewi Haryani, Ni Ketut Dewi Niluh Budi Artini Nisfaun Safitriana Nova Damayanti Novika Nur Asrinawaty Nur Hikma Nurfianti Nurhayati N Mangopo Nurvita Nurvita Nurzakia Nurzakia Orryani Lambui Rahayu Rahayu Rahmania Rahmania Rahmiati Rahmiati Ramadanil Pitopang Rifka Rifka Rizki Purnamasari Rizkita Rachmi Esyanti Roliana Roliana Saparudin Saparudin Septian, I Gede Nano Setia Ayu Lestari Sitti Ardianti Sitti Masyitah Sri Utami Subhan nuradzan Tamzil, Moh Hasil Tapaul Rozi Tjandra Anggraeni Tjandra Anggraini Tri Marsha Hasibuan Triana, Nina Risnanda Trismansyah Trismansyah Wahyu Harso Wahyu Hidayat Wasilahturahman Widiawati Yulia Astuti Yusran Yusran Yustilawati, Eva