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Journal : Biocelebes

PENGAMATAN PERTUMBUHAN TANAMAN BAYAM (Amaranthus tricolor L.) PASCA APLIKASI BIOFERTILIZER (BAHAN AKTIF Aspergillus sp.) SEDIAAN CAIR Yulia Astuti; Umrah; Abdul Rahim Thaha
Biocelebes Vol. 14 No. 2 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i2.15272

Abstract

The study about the observation of spinach (Amaranthus tricolor L.) growth after the addition of liquid biofertilizer (active ingredient Aspergillussp.) had been conducted in Taman Pangan Gizi Langaleso Village, Dolo Subdistrict, Sigi District, Central Sulawesi, and Laboratory of Biotechnology, Biology Department, Faculty of Mathematics and Natural Sciences, Tadulako University, Palu, from March to November 2018. The aim of the study was to observe the growth of spinach after the addition liquid biofertilizer (active ingredient Aspergillus sp.) along with the fermented soybean liquid waste and coconut water waste as the main media. This study was designed using the Completely Randomized Design which consisted of 7 treatments with 5 repetitions. The treatments were arranged as follows: P0 (without the addition ofbiofertilizer, as the negative control), P1 (2.5%biofertilizer addition), P2 (5%biofertilizer addition), P3 (7.5%biofertilizer addition), P4 (10%biofertilizer addition), P5 (12.5%biofertilizer addition), P6 (NPK addition, without the addition of biofertilizer, acting as the positive control). The results showed that treatment P4 was indicated as the best growth among any other treatments as well as the control, based on the parameters of the height of the plants (10 cm), the number of leaves (8.35 leaf blades), the dry weight of biomass (1.95 g), the fresh weight of canopy (4.94), the fresh weight of leaves (2.07 g), the dry weight of leaves (0.22 g), the fresh weight of roots (2.11 g), the dry weight of roots (0.24 g) and the length of the roots (12.2 cm).
PENERAPAN PUPUK ORGANIK CAIR (Bahan Aktif Aspergillus sp.) BERBAHAN DASAR LIMBAH CAIR TEMPE DAN AIR KELAPA PADA TANAMAN KANGKUNG DARAT (Ipomoea reptans Poir.) Miranty Sirappa; Umrah; Abdul Rahim Thaha
Biocelebes Vol. 14 No. 3 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i3.15423

Abstract

Liquid Organic fertilizer is a fertilizer produced from organic materials in the form of liquid preparations. Research on the application of liquid organic fertilizer (active ingredient Aspergillus sp.) is based on liquid waste of Tempe and coconut water on the Kangkung land plant (Ipomoea reptans Poir.), this research has been conducted in March to November 2018 in the village Langaleso, Dolo subdistrict, Sigi Regency, Biotechnology Laboratory of Biology Department of Mathematics and Natural Sciences of Tadulako University. This research aims to observe the growth of plant Kangkung post application of liquid organic fertilizer (active ingredient Aspergillus sp.). The research designed in the complete random plan (RAL) consists of seven types and five times the replay. Composition of treatment; P0 (without the application of POC, control), P1 (Application of POC 2.5%), P2 (Application of POC 5%), P3 (Application of POC 7.5%), P4 (Application of POC 210%), P5 (Application of POC 12.5%), P6 (NPK application, without POC). Description POC Liquid Organic fertilizer (active ingredient Aspergillus sp.). The observation results showed the best growth on the implementation of P1 based on the fresh weight of the heading (8.47 g), the fresh weight of biomass (6.02 g), the fresh weight of the leaves (4.66 strands), the fresh weight of the roots (5.72 g). The P2 is based on the root length (27.10 cm). P4 treatment based on the high average plant (27.34 cm), the number of leaves (14.90 strands), dry weight of the roots (1.61 g). P6 treatment based on leaf dry weight (2.07 g).
PENGAMATAN PERTUMBUHAN DAN PRODUKSI TANAMAN BAWANG MERAH (Allium ascalonicum L.) VARIETAS LOKAL LEMBAH PALU PASCA APLIKASI BIOKOMPOS Febiyanti; Umrah
Biocelebes Vol. 14 No. 3 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i3.15424

Abstract

Biocompost is an organic fertilizer term that is made from the process of decomposition of organic material through the addition of decomposers. This research has been carried out at the Botanical Gardens Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University, Palu, from March to May 2019. The research waere aimed to determine the growth and production of shallot plants Allium ascalonicum L. and the appropriate dosage of biocompost to increase the growth and production of shallot plants of A. ascalonicum L. local varieties of the valley Hammer post biocompost application. The method used in this study is Complete Randomized Design method. The results of the observations can be concluded that biocompost made from livestock waste from goat chickens, cattle with oyster mushroom waste supplements made from active Aspergillus sp. Does not increase the growth and production of onion plants due to biocompost doses that are too high. Provision of 100 and 500 grams of biocompost / polybag has the lowest tuber dry weight.
EKSPLORASI BAKTERI LOKAL PENGHASIL ANTIMIKROBA DI CAGAR ALAM TANJUNG API TELUK TOMINI SULAWESI TENGAH, INDONESIA Alwi Millang; Widiawati; Umrah; Orryani Lambui
Biocelebes Vol. 15 No. 1 (2021)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v15i1.15472

Abstract

ABSTRACT Tanjung Api Nature Reserve is a headland located in Tomini Bay Ampana District Tojo Una-Una Regency, Central Sulawesi Province. This study aims to obtain local bacteria isolates as antimicrobial producers. The method used in this research is purposive sampling and screening method using "Agar Dua Lapis" (Double Layer Method). Testing antimicrobial potency using the method to make Well Diffusion (well) using three pathogens of microbial tests (S. aureus, E. coli, and C. albicans). In each well hole is given 500 μL of bacterial isolates each and incubated at a temperature of 37 oC for 24 hours and continued for 48 hours. The observed parameter is a clear zone formed around the well. Antimicrobial-producing bacteria screening results. obtained as many as 24 isolates of bacteria. Testing of antimicrobial potency by looking at the resulting inhibitory zone is obtained five isolates namely S2U1, S3U3, S7U3, S9U3, and S10U3 are the highest isolates of the bland zone of 12.25 mm but only against S. aureus, while S9U3 is the best isolate because it is able to inhibit the four test microbes namely S. aureus by 5.50 mm, E. coli 9.25 mm, and C. albicans 5.50 mm. Keywords: Exploration of bacteria, antimicrobials, and Nature Reserve
POTENSI EKONOMI PEMANFAATAN LIMBAH BATANG PISANG DAN LIMBAH JERAMI JAGUNG SEBAGAI SUBSTRAT DASAR UNTUK PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH umrah umrah; I Nengah Suwastika; Lina Mahardiana; Meryany Ananda; Novika
Biocelebes Vol. 15 No. 1 (2021)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v15i1.15490

Abstract

Pertumbuhan miselium jamur tiram putih (Pleurotus ostreatus) pada substrat (batang pisang dan batang jagung) telah dilakukan di Laboratorium Mikrobiologi, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam. Penelitian ini bertujuan untuk mengetahui: (a) pertumbuhan P. ostreatus pada substrat yang ditambahkan dengan limbah air kelapa dan limbah tempe; (b) karakteristik pertumbuhan P. ostreatus pada perlakuan berbagai jenis substrat. Penelitian ini dirancang berdasarkan Rancangan Acak Lengkap (RAL) yang terdiri dari enam perlakuan dan empat ulangan. Komposisi dari perlakuan adalah rasio antara batang pisang : batang jagung yaitu P1 (100%), P2 (80%: 20%), P3 (60%: 40%), P4 (40%: 60%), P5 (20% : 80%), P6 (100%). Hasil penelitian menunjukkan bahwa Perlakuan P6 lebih baik dibandingkan dengan perlakuan lain pada pertumbuhan miselium.
POTENSI EKONOMI LIMBAH TANDAN KOSONG KELAPA SAWIT DAN DEDAK PADI SEBAGAI MEDIA TUMBUH MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus) umrah umrah; Lina Mahardiana; Meryany Ananda; Eny Yuniati; Mutma Inna
Biocelebes Vol. 15 No. 2 (2021)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v15i2.15607

Abstract

Penelitian ini perlu di lakukan karena pada limbah tandan kosong kelapa sawit dan dedak padi merupakan sumber nutrisi yang bernilai ekonomi tinggi yang dapat dimanfaatkan sebagai media pertumbuhan jamur tiram putih (Pleurotus ostreatus). Tujuan penelitian ini adalah (1) mengetahui pertumbuhan miselium jamur tiram putih (P. ostreatus) pada substrat limbah tandan kosong kelapa sawit dan dedak padi; (2) mengetahui karakteristik pertumbuhan jamur tiram putih (P. ostreatus) pada perlakuan jenis substrat yang berbeda. Penelitian ini telah di lakukan dalam rancangan acak lengkap (RAL) yang terdiri tujuh perlakuan dan tiga kali pengulangan. Susunan perlakuan merupakan perbandingan antara tandan kosong kelapa sawit : dedak padi, yakni P1 (50% : 50%), P2 (45% : 55%), P3 (40% : 60%), P4 (35% : 65%). P5 (30% : 70%), P6 (25% : 75%), P7 (serbuk geragaji 70%, dedak padi 20%, tepung jagung 10%). Adapun hasil penelitian menunjukkan laju pertumbuhan miselium tercepat pada perlakuan P1 yaitu 89 mm dan terendah pada perlakuan P5 yaitu 80 mm. Jumlah koloni tertinggi pada perlakuan P5 yaitu 1,4x1012 CFU/g dan terendah pada perlakuan P4 yaitu 0,4x1012 CFU/g. Waktu inkubasi paling cepat pada perlakuan P4 yaitu 28 hari dan terendah pada perlakuan P7 yaitu 33 hari.
KARAKTERISTIK PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus) PADA MEDIUM DASAR JERAMI DAN TONGKOL JAGUNG umrah umrah; I Nengah Suwastika; eny Yuniati; Rifka Rifka; Nurvita Nurvita
Biocelebes Vol. 15 No. 2 (2021)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v15i2.15621

Abstract

Laju pertumbuhan miselium jamur tiram putih (Pleurotus ostreatus (Jacq) P. Kumm) dengan medium dasar jerami dan tongkol jagung telah dilaksanakan di Laboratorium Mikrobiologi Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam. Tujuan Penelitian; (a) Untuk mengamati pertumbuhan jamur tiram putih (Pleurotus ostreatus) pada formulasi media limbah jerami dan tongkol jagung; (b) Untuk mengetahui perbandingan dosis limbah jerami dan tongkol jagung yang dapat memberikan pertumbuhan terbaik terhadap jamur tiram putih. Penelitian ini didesain berdasarkan Rancangan Acak Lengkap (RAL) yang terdiri dari 7 perlakuan dan 3 kali ulangan. Susunan perlakuan merupakan perbandingan antara Serbuk jerami jagung : serbuk Tongkol Jagung yakni P1 (Serbuk Jerami 100%, tanpa Serbuk Tongkol Jagung), P2 (80% + 20%), P3 (60% + 40%), P4 (40% + 60%), P5 (20% + 80%), P6 (Serbuk Tongkol Jagung 100%, tanpa Serbuk jerami), P7 (Serbuk Gergaji 70% + Dedak Padi 20% + Tepung Jagung 10%, tanpa Serbuk Jerami dan Tongkol Jagung). Parameter pengamatan; (a) Media dasar pertumbuhan; (b) Karakteristik mikroskopik hifa; (c) Laju pertumbuhan miselium; (d) Karakteristik makroskopik koloni; (e) Masa inkubasi. Hasil penelitian menunjukkan laju pertumbuhan miselium tercepat pada perlakuan P4 yaitu 103 mm dan terendah pada perlakuan P1 yaitu 75 mm. Jumlah koloni (CFU) tertinggi pada perlakuan P7 yaitu 1,6x1012CFU/g dan terendah pada perlakuan P4 yaitu 0,2x1012CFU/g. Waktu inkubasi paling cepat pada perlakuan P7 yaitu 28 hari dan terendah pada perlakuan P4 yaitu 30 hari.
PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus) PADA MEDIA FORMULA JERAMI JAGUNG DAN LIMBAH BIJI KOPI umrah umrah; Eny Yuniati; Amiruddin Kasim; Kirana Kirana
Biocelebes Vol. 16 No. 1 (2022)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v16i1.15950

Abstract

Corn straw and coffee bean waste as a medium and source of nutrients needed for the growth of mycelium white oyster mushroom (P. ostreatus). This research has been carried out at the Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. The purpose of this study was to determine the growth of mycelium and the characteristics of white oyster mushroom (P. ostreatus) in the formulation of corn straw and coffee bean waste media. This study was designed in a completely randomized design (CRD), consisting of seven treatments and three replications, namely P1 (100% corn straw powder), P2 (95% corn straw powder + 5% coffee bean waste), P3 (90% corn straw powder + 10% coffee bean waste), P4 (85% corn straw powder + 15% coffee bean waste), P5 (80% corn straw powder + 20% coffee bean waste), P6 (75% corn straw powder + 25% coffee bean waste), P7 (70% sawdust + 20% rice bran + 10% corn flour as a positive control). The results showed that corn straw and coffee waste which were used for growth media of white oyster mushroom mycelium (P.ostreatus) could be used as basic media because mycelium could grow well in all treatments. The growth rate of white oyster mushroom mycelium in P3 treatment enjoyed very fast growth and P6 treatment showed very slow growth. The highest number of colonies was in treatment P3 6.40x1011CFU/grand the lowest number of colonies was in treatment P6 2.66x1011CFU/g. Treatment P3 takes a shorter time to fill the culture bottles, which is 30 days, while P6 takes a longer time to fill the culture bottles, which is 48 days.
PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus) PADA MEDIA DASAR LIMBAH KULIT SINGKONG umrah umrah; Asriani Hasanuddin; Eny Yuniati; Trismansyah Trismansyah
Biocelebes Vol. 17 No. 1 (2023)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v17i1.16440

Abstract

Cassava peel used as the basic medium for the growth of mycelium of white oyster mushroom (Pleurotus ostreatus). The purpose of this study : to determine the growth of white oyster mushroom mycelium (P. ostreatus) and to determine the ratio of basic media that can provide the best growth of white oyster mushroom mycelium. This study was designed in a completely randomized design (CRD) consisting of seven treatments and three replications, namely P1 (100% cassava peel base medium), P2 (MD 80% + SG 20%), P3 (MD 60% + SG 40% ), P4 (MD 40% + SG 60%), P5 (MD 80% + SG 10%), P6 (SG 100%), P7 (70% sawdust, 20% rice bran + 10% corn flour). The results of observation this research of the fastest growth at P4, P5 and the lowest at P6, were seen from the observation of mycelium growth. Colony stages on Potato Sucrose Agar (PSA) media, the results obtained were the highest number of colonies (CFU) in P1 treatment 1.83 x 1012 CFU/g and the lowest in P6 treatment 0.8 x 1012 CFU/g. The fastest storage time in treatment P4, P5 and the lowest in treatment P6.
MEDIA DASAR LIMBAH DAUN KAKAO UNTUK PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus) Umrah; Saparudin, Saparudin; Yuniati, Eny; Ananda, Meryany
Biocelebes Vol. 17 No. 2 (2023)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v17i2.16606

Abstract

Cocoa leaf wastes as a basic medium are the nutrition that needed for the growth of white oyster mushroom mycelium (Pleurotus ostreatus). The objectives of this study were determine the cocoa leaf wastes as a basic medium for the growth of white oyster mushroom mycelium and the dosage of basic medium to encourage the best fungal growth. This research was designed in a Completely Randomized Design (CRD), consisting of seven treatments and three replications, namely P1 (Basic Media of 100% cocoa leaf waste), P2 (Basic Media 90% + Suplement 10%), P3 (Basic Media 80% + Suplement 20 %), P4 (Basic Media 70% + Suplement 30%), P5 (Basic Media 60% + Suplement 40%), P6 (Basic Media 50% + Suplement 50%), P7 (Sawdust 70%, 20% rice bran + flour corn 10%). The method that used in this study were media preparation, drying, grinding and formulation. The parameters that observed were the basic media, microscopic characteristics of hyphae, mycelium growth curve and macroscopic characteristics of colonies. The results of P1 and P7 showed the best growth for mycelium, observation of mycelium growth were done every 3 days. The isolation stage was carried out on Potato Sucrose Agar (PSA) media, the results obtained that P1 was 0,1 x 1012 CFU/g, P2 was 0,4 x 1012 CFU/g, P3 was 0,3 x 1012 CFU/g, P4 as much as 0,7 x 1012 CFU/g, P5 as much as 0,6 x 1012 CFU/g, P6 as much as 0,4 x 1012 CFU/g, P7 as much as 2,4 x 1012 CFU/g, respectively. Cocoa leaf waste are potentially as a growth medium for mycelium of white oyster mushroom.
Co-Authors A. St. Latifah Humairah A AA Sudharmawan, AA Adhiwijaya, Ardian Adi Putra, Andi Budiyanto Aisah Jamili Alwi Alwi Alwi Millang Amalia Purnamasari Zainal Amiruddin Kasim Andi Lindhemuthianingrum Annawaty Annawaty, Annawaty Asfiani, Asfiani Asri Ismail Asri Pirade Paserang Asriani Hasanuddin Asrul Asrul Asrul Asrul Azzahra, Andini Shafa Arezy Azzahra Bidayanti Ruru Budi Indarsih, Budi Burhan, Kurnia Dana Sulistia Dewi, Ni Made Aryani Saraswati Dhini Arwindah Dian Sri Anugrah Djoko Kisworo Eka Fitriani Eny Yuniati Evisilvia Evisilvia Fahri Fahri Febiyanti Firdasari Firdasari Firji Della Sugeha Fitri Kurnianingsih Fitriani Fitriani Fitriani Husain Gina, Fina Alimatul Hardianti Lestari Hamid Hasriani Hasriani Humaero, Siti I Nengah Suwastika I Nyoman P. Aryantha Ika Merdeka Wati Ikha Wahyuni Ilham Bakri Abdul Rahim Thaha,Isrun, Ilham Bakri Irawan, Adit IRWAN LAKANI Jamaluddin Jamaluddin Jaya, I Nyoman Sukartha Jesica Pasaribu Juliana Juliana Kasman Sulaiman Khadijah Kirana Kirana Laksono Trisnantoro Lamadjido, Sri Rahayu Latifah Merdekawaty Lina Mahardiana Makmur, Baitul Mardiana Upara Maulana, Ardian Maulida, Nurhidayatul Meryany Ananda Miranty Sirappa Miswan Miswan Moh. Iqbal, Moh. Mufida Al Idrus Muh. Arsal N. Maddo Muhaemin Muhaemin Muhammad Alwi Muhammad Amiruddin Muhammad Saleh Muhammad Yusril Basir Murnawati Murnawati Musdalifa Musdalifa Musjaya M. Guli Mustafid Rasyiid Mutma Inna Mutmainah Mutmainah Mutmainah Mutmainah Mutmainah Mutmainah Naksabandi, Roydatul Robbi Ni Ketut Dewi Haryani, Ni Ketut Dewi Niluh Budi Artini Nisfaun Safitriana Nova Damayanti Novika Nur Asrinawaty Nur Hikma Nurfianti Nurhayati N Mangopo Nurvita Nurvita Nurzakia Nurzakia Orryani Lambui Rahayu Rahayu Rahmania Rahmania Rahmiati Rahmiati Ramadanil Pitopang Rifka Rifka Rizki Purnamasari Rizkita Rachmi Esyanti Roliana Roliana Saparudin Saparudin Septian, I Gede Nano Setia Ayu Lestari Sitti Ardianti Sitti Masyitah Sri Utami Subhan nuradzan Tamzil, Moh Hasil Tapaul Rozi Tjandra Anggraeni Tjandra Anggraini Tri Marsha Hasibuan Triana, Nina Risnanda Trismansyah Trismansyah Wahyu Harso Wahyu Hidayat Wasilahturahman Widiawati Yulia Astuti Yusran Yusran Yustilawati, Eva