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Totok Wianto
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Journal : Pharmascience

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FTIR and Chemometrics Application on Determination of Total Flavonoid Content of Pasak Bumi Root Extract (Eurycoma longifolia Jack.) Liling Triyasmono; Ana Ulfah; Muhammad Ikhwan Rizki; Khoerul Anwar; Totok Wianto; Heri Budi Santoso
Jurnal Pharmascience Vol 7, No 2 (2020): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v7i2.7923

Abstract

The combination of FTIR and chemometrics is an alternative method on determination of total flavonoid content of pasak bumi (Eurycoma longifolia Jack.) root extract. This study aims to determine the method of FTIR and chemometrics can be used for determination of total flavonoid content and determine the total flavonoid content of E. longifolia root extract using FTIR and chemometrics. The samples from three growing area were determined their flavonoid content by colorimetric method and measured their absorbance by FTIR spectrophotometer. The analysis was done by PCA chemometrics to grouping IR spectra based on growing area and PLSR to determine prediction model of total flavonoid content of E. longifolia root extract. The best grouping and prediction model is shown by IR spectra in the range of wavenumbers 1800-1540 cm-1 with  total variation is 99%  and prediction model with equation y = 0.995x + 0.002 (R2 calibration = 0.995; R2 validation = 0.970; RMSEC = 0.008; RMSECV = 0.021). The total flavonoid content of E. longifolia root extract (% b/b ± SD) from Mandiangin, Condong, and Sabuai is 0.225 ± 0.009; 0.437 ± 0.007; and 0.466 ± 0.016 (R2 = 0.995 and RMSEP = 0.008). Based on this, the combination of FTIR and chemometrics can be used to predict the total flavonoid content of unknown E. longifolia root extract
Co-Authors Abdul Gafur Abdulah Hamdi Abdulah Hamdi Agung Nugroho Ahmad Rusadi Arrahimi - Universitas Lambung Mangkurat) Ahmad Rusadi Arrahimi - Universitas Lambung Mangkurat) Akhmad Faisal Akhmad Faisal Ana Ulfah Andy Mizwar Anny Sulaswatty, Anny Apriana, Susi Azizah, Laela Azwari, Ayu Riana Sari Azwari, Ayu RianaSari Darminto . Darminto Darminto Dewi Amelia Widiyastuti Dodon Turianto Nugrahadi Egi Agustian Erdiansyah Rezamela, Erdiansyah Erlina Natasya Kurniasari Fahrudin, Arfan E Fitriana, Iin Noor Gunawan Gunawan Gunawan Gunawan Gusti Atika Urfa Gusti Rusmayadi Hafiizh Prasetia, Hafiizh Hendris Hendarsyah Kurniawan, Hendris Hendarsyah Heri Budi Santoso Husna, Wardatul Husnul Madihah, Husnul Idiannor Mahyudin Intan Fitrianti, Intan Ishaq Ishaq Ishaq Ishaq Janah, Raihul Karmaili, Karmaili Kartika, Listiana Khoerul Anwar Khoerul Anwar Liling Triyasmono Manik, Tetti Novalina Maya Safitri Meta Widyayanti Meta Widyayanti Minarto, Ori Muhaemina, Maulanie Muhammad Ikhwan Rizki Muhammad Itqan Mazdadi MUHAMMAD NUGROHO, MUHAMMAD Muhammad Rif'an Abdi Muhammad Saukani Muhammad Solih Afif Muthia Elma Nabila, Nisrina Najmi, M. Ikhwan Nasrulloh, Amar V Nasrulloh, Amar Vijai Ninis Hadi Haryanti, Ninis Hadi Nova Annisa, Nova Nurma Sari Nurma Sari, Nurma Oktafiansyah, Rahmad Ori Minarto Ori Minarto Ori Minarto Pahmiansyah Pahmiansyah Pahmiansyah, Pahmiansyah Rachman, Rezky Rachmadhany Raihul Janah Raisa Kusuma Dewi Raisa Kusuma Dewi Ramadhoni, Benni F Reida, Rina Rina Reida Saragih, Triando Hamonangan Saukani, Muhammad Seftina, Rahmi Sembiring, Rinawati Simon Sadok Siregar, Simon Sadok Sri Cahyo Wahyono Suarso, Eka Sudarningsih Sudarningsih Sudarningsih Sudarningsih Sudarningsih Sudarningsih Sudarningsih Sudarningsih Sudarningsih Sudarningsih Sudarningsih Sudarningsih Sudarningsih Sudarningsih Suminar Pratapa Sunardi Sunardi Sunardi, Ph.D., Sunardi Suryajaya Suryajaya, Suryajaya Utami Irawati Utomo, Edy Setyo Wahyono, Sri C Wardatul Husna Widya Utama Widya Utama