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All Journal Jurnal Veteriner Bioscience Biota Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Journal of Tropical Biodiversity and Biotechnology Jurnal Biodjati Molecular and Cellular Biomedical Sciences (MCBS) Animal Production : Indonesian Journal of Animal Production Biota Journal of Aquaculture and Fish Health Jurnal Inovasi Pembelajaran Karakter Berkala Penelitian Hayati Prosiding Konferensi Nasional PKM-CSR Jurnal Pengabdian Masyarakat Charitas Rumphius Pattimura Biological Journal Berkala Ilmiah Biologi

Slamet Widiyanto

Department Of Tropical Biology, Faculty Of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Author-ID : 415791

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary

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Aseet Sosial Pengembangan Pariwisata Pedesaan Tangguh Bencana Berkelanjutan Muhammad Muhammad; Slamet Widiyanto
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 3 (2020): Peran Perguruan Tinggi dan Dunia Usaha Dalam Pemberdayaan Masyarakat Untuk Menyongsong
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Kegiatan Pengabdian Kepada Masyarakat (PKM) yang berlokasi di Desa Kalibening, Kecamatan Dukun, Kabupaten Magelang yang dilaksanakan sejak tahun 2013-2018 telah banyak pengetahuan yang didapatkan dari proses belajar bersama masyarakat yang ditinjau dari berbagai bidang keilmuan. Fakta yang melatarbelakangi pemilihan lokasi kelompok sasaran merupakan kawasan rawan bencana erupsi Gunung Merapi, tingkat pendidikan yang masih rendah, kemiskinan, masyarakat tidak berdaya dan lain-lain. Tujuan utama dalam kajian pelaksanaan Pengabdian Kepada Masyarakat adalah sebagai upaya membangun kesepahaman terhadap bentuk pembangunan melalui pemberdayaan masyarakat yang diharapkan dapat meningkatkan kapasitas masyarakat (capacity building) sehingga mampu memenuhi harapan dan kebutuhan masyarakat. Asset social (social mapping) pada kelompok sasaran dapat mengetahui rendahnya tingkat pengetahuan, kemiskinan dan keterampilan yang dimiliki masyarakat dan kelompok sasaran mengakibatkan mereka tidak mampu mengenali apa yang dibutuhkan dalam meningkatkan kapasitasnya beserta potensi besar yang dimilikinya. Metode dan analisis yang dilakukan dalam kajian ini lebih terfokus pada Focus Group Disscussion (FGD), wawancara mendalam, quisioner dalam upaya menjaring dan menemukenali tingkat partisipatif masyarakat dan wawancara secara terstruktur. Penelitian menggunakan rentang waktu (masa pelaksanaa) pada periode tahun 2014-2018 melalui skema Pengembangan Desa Binaan (Desbin), skema yang dikaji berdasarkan urutan waktu secara berurutan. Hasil penelitian menunjukan tingkat partisipatif yang tinggi dan mengetahui modal sosial berupa: modal ekonomi, modal alam, modal fisik, modal sumber daya manusia modal pengetahuan dan berkontribusi terhadap pengembangan potensi desa berupa pengembangan pariwisata berbasis masyarakat tangguh bencana yang berkelanjutan. Kesimpulan dan hasil perhitungan modal sosial yang didapatkan dengan nilai tertinggi sebesar modal sumber daya alam sebesar (3,9), modal sosial (3,0), modal finansial (3,2), modal fisik merupakan modal yang terendah dengan nilai (2,6).

Maturation of Female Yellow Rasbora (Rasbora Lateristriata Bleeker, 1854) Using Oodev at Different Doses in Feed Rey, Juniman; Widiyanto, Slamet; Retnoaji, Bambang
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.75916

The current high demand for Yellow rasbora (Rasbora lateristriata) is not supported by the availability of captured Yellow rasbora in nature. Aquaculture is the most rational way of utilizing biological natural resources. In intensive aquaculture, it is necessary to optimize all processes that occur in aquaculture, including hatchery. However, the common problem that often happens in hatchery activities is spawning which depends on the season. The hormonal manipulation technique is an appropriate way to stimulate gonadal maturation. Oodev is a hormonal combination of pregnant mare serum gonadotropin and anti-dopamine to stimulate gonadal maturation. The purpose of this study was to determine the effectiveness of using the Oodev with different doses in feed to accelerate gonad maturation of female Yellow rasbora. The study was carried out with four treatments and three replications in 21 days with different doses of Oodev, such as; A (Feed without Oodev), B (0.5 mL/kg feed), C (1 mL/kg feed) and D (2 mL/kg feed). The parameters observed in this study were gonad maturity level, histological structure of ovary, gonadosomatic index, fecundity, and diameter of eggs. The results showed that the dose of Oodev at 1.0 mL/kg feed was an effective dose to optimize the gonad maturity of female Yellow rasbora. This is proven by the highest results shown on all parameters, such as; the maturity level in the IV phase, histological structure of the ovary which showed the dominance of the oocyte maturation phase, gonadosomatic index of 14.014%, the fecundity of 721 eggs, and egg diameter of 0.865 mm. In conclusion, using Oodev in feed at a dose of 1.0 ml/kg of feed for 21 days is an effective dose to optimize the maturation of female Yellow rasbora.

Sistem Memori dan Pembelajaran pada Mamalia susilowati, ari; Widiyanto, Slamet; Kusindarta, Dwi Liliek; Wijayanti, Nastiti
Berkala Ilmiah Biologi Vol 15 No 3 (2024)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v15i3.17604

Memory formation in mammals involves complex stages, from initial learning to long-term storage. This review highlights key memory-related genes and proteins such as SYNGAP1, Arc/Arg3.1, BDNF, FOXP2, COMT, NR3C1, KIBRA, H-Ras, ERK1/2, and other genes. Mammals serve as important models in memory research due to their evolutionary proximity to humans, providing insights into brain structures such as the hippocampus and prefrontal cortex, which are crucial in memory processes. Molecular mechanisms such as transcription, translation, synaptic plasticity, long-term potentiation (LTP), and long-term depression (LTD), as well as neurotransmitters like glutamate, GABA, acetylcholine, dopamine, and serotonin, are comprehensively discussed with a focus on small, large mammals, and humans. Neurotransmitters affect various functions, including emotion, cognition, memory, learning, and motor functions in test animals. Disruptions in neurotransmitter homeostasis have been linked to numerous neurological and neurodegenerative disorders, highlighting the need to understand the complex mechanisms behind memory in mammals. A brief overview of genes and neurotransmitters related to memory and learning will provide insights and considerations in basic neurobiology and biomedical research.

Histopathological Effects of Mangosteen (Garcinia mangostana L.) Peel Decoction on Betta Fish (Betta sp.) Liver Ariesti, Wiwin; Aeniah, Siti; Halim, Shuha Ma’muriyah; Sofyantoro, Fajar; Wijayanti, Nastiti; Retnoaji, Bambang; Nuriliani, Ardaning; Saragih, Hendry T.S.S.G.; Rohmah, Zuliyati; Widiyanto, Slamet; Pusparini, Nur Ainun Oktavia; Empra, Desi Eka Putri; Septriani, Nur Indah
Biota Vol 17 No 2 (2024)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar

Mangosteen (Garcinia mangostana L.) peel contains bioactive compounds known for their health benefits, yet potential toxicity at certain doses remains a concern. This study evaluates the histopathological effects of mangosteen peel decoction on the liver of Betta fish (Betta sp.), a sensitive model organism. Mangosteen peel decoction was prepared and administered to Betta fish at concentrations of 5, 25, and 50 ppm, with a control group receiving no treatment. Fish were observed for changes in swimming activity and appetite over five days. Liver tissues were collected, processed, and analyzed histologically to assess tissue damage including vacuolization, pyknosis, hemorrhage, and necrosis. Data were analyzed using the Kruskal-Wallis and Mann-Whitney tests. Behavioral analysis indicated a dose-dependent reduction in swimming activity and appetite in treated groups. Histopathological examination revealed significant liver damage across all treatment groups, with higher concentrations of decoction correlating with increased hemorrhage, pyknosis, and necrosis. Vacuolization was highest in the control group and lowest in the 50-ppm group. The overall hepatic damage was categorized as moderate, with the control group showing the least damage. Mangosteen peel decoction induced significant hepatic damage in Betta fish, highlighting the cytotoxic effects at higher doses. The observed behavioral and histopathological changes underscore the need for careful consideration of decoction concentrations to avoid adverse effects in aquatic organisms. This study provides crucial insights into the toxicological impacts of mangosteen peel decoction on fish liver health, emphasizing the importance of dose regulation in practical applications. Further research is recommended to explore protective measures and alternative treatments to mitigate liver damage.

n-Hexane Fraction of Cucumis melo L. Cultivar Gama Melon Parfum: An in vitro Study in MCF7 and T47D Cells Line Salamah, Rohmi; Wijayanti, Nastiti; Widiyanto, Slamet
Molecular and Cellular Biomedical Sciences Vol 9, No 2 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i2.558

Background: Cucumis melo a melon species, typically has a sweet taste. Some cultivars are known for their distinctive bitter flesh due to its higher levels of cucurbitacin. Cucurbitacin is semipolar compound that has anticancer properties. However, the anticancer effects of cucurbitacin from gama melon parfum (GMP) have not been widely studied. The use of n-Hexane as a non-polar solvent in GMP melon fractionation is to dissolve the non-polar parts of the plant. However, Cucurbitacin was found in the n-hexane fraction of Cucurbita pepo L. Therefore, this study will investigate the presence of Cucurbitacin in the n-Hexane fraction and its effects on breast cancer cells T47D and MCF7.Materials and methods: Dry simplicia of GMP melon fruit were macerated using methanol and fractionated using n-hexane. The presence of cucurbitacin was detected using the high-performance liquid chromatography (HPLC) method. Cell cytotoxicity tests were assessed using the MTT assay, with concentrations of 7.8125, 15.625, 31.25, 62.5, and 125 µg/mLResults: Cucurbitacin compounds were detected in the n-hexane fraction at a concentration of 7.6 µg/mL per 10 mg of n-hexane fraction. MCF7 cell viability was lower than that of T47D cells across all concentrations tested. MCF7 cell viability was below 50% at a concentration of 62.5 µg/mL. In contrast, T47D cell viability remained at 100% even at the highest concentration of 125 µg/mL. The IC50 value of MCF7 cells was 43.5 µg/mL.Conclusion: The cucurbitacin content in the n-Hexane fraction was 7.6 µg/mL per 10mg fraction. At this concentration, it moderately inhibits the proliferation of MCF7 cells.Keywords: gama melon parfum, cucurbitacin, HPLC, T47D, MCF7

Peningkatan Mutu “Papie-Sue” melaui Pendekatan GMP, SSOP, dan HACCP” Siswara, Hamzah Nata; Huda, Khoirul; Wulandari, Kartika; Widiyanto, Slamet; Kholil, Abdul
Jurnal Pengabdian Masyarakat Charitas Vol. 4 No. 02 (2024): Jurnal Pengabdian Masyarakat Charitas
Publisher : Program Studi Teknik Industri, Fakultas Teknik, Universitas Katolik Indonesia Atma Jaya Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/charitas.v4i02.6110

Papie-Sue is a diversification of agricultural and livestock products. Papie-Sue means banana pie with etawa milk. The main raw material for pie crust comes from banana flour, and the raw material for vla comes from etawa goat's milk. In addition, there are variations in flavors from local fruits, namely starfruit and durian. All raw materials and variations in flavors are local products of Bojonegoro. Because this product is processed from fresh ingredients without preservatives, a method is needed to maintain product quality until it reaches consumers. To improve the quality of Papie-Sue, GMP, SSOP, and HACCP are implemented through training and mentoring for the production team. The implementation of a food safety system based on GMP, SSOP, and HACCP can improve product quality by avoiding food contamination both biologically, chemically, and physically. Potential contamination will be identified to carry out prevention processes during the production process. Training participants consist of a production team of 15 people. The implementation of this food safety system is expected to be one way in the process of increasing the scale of the Papie-Sue business at SMKN Ngambon.

The Use of OODEV® in Improving the Reproductive Performance of Male Yellow Rasbora Fish Rasbora lateristriata (Bleeker, 1854) Hurul Ein; Retnoaji, Bambang; Slamet Widiyanto
Journal of Aquaculture and Fish Health Vol. 13 No. 2 (2024): JAFH Vol. 13 No. 2 June 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i2.46975

A local commodity, Yellow rasbora fish Rasbora lateristriata (Bleeker, 1854), can potentially be used as a food resource by the Kulon Progo community. Added to that, cultivation or aquaculture is known to optimize this potential. Furthermore, Aquaculture will also prevent illegal fishing in nature, where yellow rasbora fish are listed as vulnerable species in the IUCN Red list. This study induced male yellow rasbora fish using the hormone OODEV® injection, which comprises the hormone Pregnant Mare Serum Gonadotropin (PMSG) and anti-dopamine. PMSG is a hormone that contains FSH as well as LH in it. Yellow rasbora fish were nurtured to the age of six months and treated with four different treatments (dose) for a month, namely 0 mL/Kg, 0.25 mL/Kg, 0.5 mL/Kg, and 0.75 mL/Kg. Observed parameters included motility (movement and duration) as well as sperm count, sperm viability, sperm morphology, sperm morphometry, gonadosomatic index (GSI), and survival rate (SR). Data obtained were analyzed using SPSS with the One-way ANOVA test and continued with Duncan's test if the results were significant. The obtained results had no significant effect on the IGS value (p>0.05), with the highest value found at a dose of 0.75 mL/Kg. In addition, administration of OODEV® also improved sperm quality with the best results at 0.75 mL/Kg in terms of motility with the highest progressive movement compared to other doses but not significant (p>0.05) compared to the control, and significantly (p<0.05) longest movement duration compared to other doses, while in terms of morphometry it had the second widest middle piece area but not significant (p>0.05) compared to other doses, widest tail area but not significant (p>0.05) compared to other doses except with 0.25 mL/Kg, longest tail length (p>0.05) but not significant with other doses except with 0.25 mL/Kg , and significantly (p<0.05) had the longest sperm total length. Every dose showed a 100% percentage of survival rate.

Hemotoxicity of Hairy Fig (Ficus hispida L.f.) Fruits on Male Wistar Rats (Rattus norvegicus Berkenhout, 1769) Fitria, Laksmindra; Sari, Annisa Lintang; Handayani, Lisa; Widiyanto, Slamet
Jurnal Biodjati Vol 7 No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.19241

Hairy fig (Ficus hispida) fruits (HFF) are widely consumed as food and traditional medicine in several West Asian countries, both the unripe fruit (UHFF) and ripe fruit (RHFF). However, they are not commonly utilized in Indonesia. Acute oral toxicity studies reported No Observed adverse effect level (NOAEL). Further, a reproductive toxicity study found that UHFF boosted spermatogenesis and increased the quality and quantity of spermatozoa. Meanwhile, RHFF exhibited the opposite effects. To provide comprehensive information from the previous study, this research was conducted to evaluate the hemotoxicity of UHFF and RHFF about their impact on the male reproductive system. Nine Wistar rats were assigned into three groups: the first group received UHFF juice, the second group received RHFF juice, and the third group received distilled water as control. The volume of each treatment was 2 mL/individual/day for 77 days. On days 0, 28, and 77, blood samples were collected for routine hematological profile examination using a hematology analyzer (SysmexÂ®XP-100). Data were analyzed using a one-way ANOVA test and Duncan's test (Î±=0.05) to discover significant differences between groups and times. Results showed that consuming hairy fig fruit, especially the UHFF, had an unfavorable effect on erythrocytes resulting in hypochromic microcytic anemia. Still, there was no adverse effect on leukocytes and platelets. Anemia may have occurred due to the presence of hemotoxic compounds that interfere with the synthesis and binding of hemoglobin or because the hairy fig fruit filtrates were oxidized, thereby increasing the level of oxidative stress within the body, of which is a drop in hemoglobin levels.

Modulatory Effects of Fucoidan on Paracetamol-induced Hepatic Damage and Histological Alterations in Rasbora lateristriata Sofyantoro, Fajar; Dafa, Maula Haqul; Primahesa, Alfian; Oktaviana, Shintia; Mayani, Olvita; Simanungkalit, Eben Ezer; Prissandi, Anthera Al Firdaus; Nuriliani, Ardaning; Retnoaji, Bambang; Saragih, Hendry T. S. S. G.; Rohmah, Zuliyati; Widiyanto, Slamet; Wijayanti, Nastiti; Septriani, Nur Indah
Biota Vol 12 No 1 (2026): Jurnal Biota 2026
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v12i1.27845

Paracetamol overdose is a well-documented cause of hepatic injury across vertebrate species, including teleost fish. This study aimed to evaluate the hepatoprotective potential of fucoidan, a sulfated polysaccharide derived from brown algae, against paracetamol-induced liver damage in Rasbora lateristriata. Fish were divided into five experimental groups and exposed to paracetamol (3 mg/L), either alone or in combination with fucoidan at concentrations of 50, 100, or 300 µg/mL, for seven days. Histopathological evaluation of liver tissues was performed using hematoxylin–eosin staining, with semi-quantitative scoring focused on hydropic degeneration, nuclear pyknosis, and necrosis. The results demonstrated that paracetamol exposure induced moderate hepatocellular injury, characterized by cytoplasmic vacuolization, apoptotic nuclear alterations, and necrotic lesions. Co-treatment with fucoidan at 300 µg/mL was associated with reduced severity across all histopathological parameters, indicating partial hepatoprotective effects. In contrast, the 50 µg/mL fucoidan group exhibited paradoxically severe hydropic degeneration despite the absence of pyknosis and necrosis, suggesting a delayed or altered injury profile. Intermediate outcomes were observed at 100 µg/mL. Overall, fucoidan exhibited dose-dependent but inconsistent hepatoprotective effects. The observed histological variability across concentrations suggests that protection may be influenced by factors such as bioavailability, cellular uptake, or interactions with intracellular stress pathways. These findings highlight the need for further mechanistic investigations before fucoidan can be considered a reliable hepatoprotective agent in aquatic toxicology.

Histopathological Analysis of Liver Damage in Rasbora lateristriata Fish Induced by Extract Butterfly Pea Flower (Clitoria ternatea) Pertiwi, Anggita Endar; Illiyin, Sirayya; Sadewo, Imran; Rahayu, Sekar; Rumansara, Papuani Samparisna; Podhi, Felisitas Moli; Sularsah, Sais; Adelfiani, Adelfiani; Retnoaji, Bambang; Sofyantoro, Fajar; Nuriliani, Ardaning; Wijayanti, Nastiti; Saragih, Hendry T. S. S. G.; Rohmah, Zuliyati; Widiyanto, Slamet; Empra, Desi Eka Putri; Ariesti, Wiwin; Septriani, Nur Indah
RUMPHIUS Vol 8 No 1 (2026): RUMPHIUS Pattimura Biological Journal (in process)
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar

Butterfly pea flower (Clitoria ternatea) is an Indonesian native plant known for its medicinal properties due to its rich content of anthocyanins, flavonoids, and ternatins. Although widely recognized for its health benefits, the long-term effects of its bioactive compounds require careful evaluation. This study aimed to investigate the potential hepatotoxic effects of butterfly pea flower extract on the liver of Rasbora lateristriata fish. Fish were exposed to 0 (control), 50, 75, and 100 ppm of butterfly pea extract for four days, with four fish allocated per group. Histological markers, including necrosis, vacuolization, pyknosis, and hemorrhage, were assessed in liver tissues. Data were analyzed using the Kruskal-Wallis test and Mann-Whitney U test at a 95% confidence level. Results indicated that butterfly pea flower extract caused significant hepatic damage, with increased severity at higher concentrations. The most pronounced damage was observed at 75 ppm, suggesting an optimal dose for inducing oxidative stress. This study highlights the potential hepatotoxicity of butterfly pea flower extract in aquatic species and underscores the need for further research to evaluate its safety for therapeutic use.

Co-Authors Abdul Kholil Adelfiani, Adelfiani Aeniah, Siti Amelia Hana Anita Yuliana Aprilia Rahmawati Ardaning Nuriliani ARI SUSILOWATI Ariesti, Wiwin Bambang Retnoaji BUDI SETIADI DARYONO Claude Mona Airin Dafa, Maula Haqul Dwi Liliek Kusindarta Eko Agus Suyono Empra, Desi Eka Putri Fajar Sofyantoro Giriani Ayu Sabilla Halim, Shuha Ma’muriyah Handayani, Lisa Hendry T.S.S.G. Saragih Hendry Tri Sakti Saragih HERA MAHESHWARI Hurul Ein I Wayan Swarautama Mahardhika Illiyin, Sirayya Irkham Widiyono Kartika Wulandari Khoirul Huda Laksmindra Fitria LUTHFIRALDA SJAHFIRDI Mayani, Olvita Muhamad Muhammad Muhammad Mulyati Mulyati Mulyati Mulyati Mulyati Sarto Nastiti Wijayanti Oktaviana, Shintia Pertiwi, Anggita Endar Podhi, Felisitas Moli Prabowo Purwono Putro Primahesa, Alfian Prissandi, Anthera Al Firdaus PUDJI ASTUTI Pudji Astuti Pusparini, Nur Ainun Oktavia Rahadian Yudo Rahayu, Sekar Rey, Juniman Rohmah, Zuliyati Rumansara, Papuani Samparisna Sadewo, Imran Salamah, Rohmi Saragih, Hendry T. S. S. G. Sari, Annisa Lintang Sarmin Sarmin Septriani, Nur Indah Septriani, Nur Indah Simanungkalit, Eben Ezer Siswara, Hamzah Nata Sularsah, Sais Syamsul Maarif

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