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All Journal Buletin Veteriner Udayana Jurnal Veteriner Bioscience Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Journal of Tropical Biodiversity and Biotechnology Molecular and Cellular Biomedical Sciences (MCBS) Animal Production : Indonesian Journal of Animal Production Biota Jurnal Inovasi Pembelajaran Karakter Berkala Penelitian Hayati Prosiding Konferensi Nasional PKM-CSR Berkala Ilmiah Biologi
Slamet Widiyanto
Department Of Tropical Biology, Faculty Of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary

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Biochemical compounds and sub-chronic toxicity test of Chlorella sp. and Spirulina sp. isolated from Glagah Coastal Water Slamet Widiyanto; Mulyati Sarto; Laksmindra Fitria; Rahadian Yudo; Eko Agus Suyono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 1 (2018): December 2018
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1127.852 KB) | DOI: 10.23869/37

Abstract

Microalgae are microscopic photosynthetic organisms found in marine and freshwater environments. This organism is a eukaryotic group of polyphyletic and very diverse. This study investigated the biochemical composition and sub-chronic test of Chlorella Sp. and Spirulina Sp. isolated from Glagah coastal water (Yogyakarta, Indonesia) as a preclinical study to create potential nutritional supplement for human health. Microalgae culture was analyzed for biochemical composition using several methods: AAS for metal analysis (Pb, Cu, Fe, Mg, Zn, and Hg), GC-MS and UV spectrophotometer for fatty acid profiles analysis (lenolenic acid, eicosatrienoic acid, arachidonic acid, linoleic acid, oleic acid, eicosenoic acid, EPA, DHA, omega 6 and 9), Alkaloids, Steroids, Tanin and Saponin. The next step is to do a sub-chronic test of microalgae biomass using experimental animals. The result shows that Spirulina sp. and Chlorella sp. contain 16 and 10 different biochemical compounds respectively. Furthermore, organic nutritional properties in both of microalgae are relatively high with a rich biochemical profile of Fe and nutritious poly-unsaturated fats (such as linolenic acid, arachidonic acid , omega 6, and 9). Regarding sub-chronic toxicity test, it was clear that Spirulina and Chlorella did not affect the blood profile. These findings suggest that both of Glagah strain microalgae are considered to have great potential as a multi-nutritional human health supplement.
Aseet Sosial Pengembangan Pariwisata Pedesaan Tangguh Bencana Berkelanjutan Muhammad Muhammad; Slamet Widiyanto
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 3 (2020): Peran Perguruan Tinggi dan Dunia Usaha Dalam Pemberdayaan Masyarakat Untuk Menyongsong
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (474.378 KB) | DOI: 10.37695/pkmcsr.v3i0.962

Abstract

Kegiatan Pengabdian Kepada Masyarakat (PKM) yang berlokasi di Desa Kalibening, Kecamatan Dukun, Kabupaten Magelang yang dilaksanakan sejak tahun 2013-2018 telah banyak pengetahuan yang didapatkan dari proses belajar bersama masyarakat yang ditinjau dari berbagai bidang keilmuan. Fakta yang melatarbelakangi pemilihan lokasi kelompok sasaran merupakan kawasan rawan bencana erupsi Gunung Merapi, tingkat pendidikan yang masih rendah, kemiskinan, masyarakat tidak berdaya dan lain-lain. Tujuan utama dalam kajian pelaksanaan Pengabdian Kepada Masyarakat adalah sebagai upaya membangun kesepahaman terhadap bentuk pembangunan melalui pemberdayaan masyarakat yang diharapkan dapat meningkatkan kapasitas masyarakat (capacity building) sehingga mampu memenuhi harapan dan kebutuhan masyarakat. Asset social (social mapping) pada kelompok sasaran dapat mengetahui rendahnya tingkat pengetahuan, kemiskinan dan keterampilan yang dimiliki masyarakat dan kelompok sasaran mengakibatkan mereka tidak mampu mengenali apa yang dibutuhkan dalam meningkatkan kapasitasnya beserta potensi besar yang dimilikinya. Metode dan analisis yang dilakukan dalam kajian ini lebih terfokus pada Focus Group Disscussion (FGD), wawancara mendalam, quisioner dalam upaya menjaring dan menemukenali tingkat partisipatif masyarakat dan wawancara secara terstruktur. Penelitian menggunakan rentang waktu (masa pelaksanaa) pada periode tahun 2014-2018 melalui skema Pengembangan Desa Binaan (Desbin), skema yang dikaji berdasarkan urutan waktu secara berurutan. Hasil penelitian menunjukan tingkat partisipatif yang tinggi dan mengetahui modal sosial berupa: modal ekonomi, modal alam, modal fisik, modal sumber daya manusia modal pengetahuan dan berkontribusi terhadap pengembangan potensi desa berupa pengembangan pariwisata berbasis masyarakat tangguh bencana yang berkelanjutan. Kesimpulan dan hasil perhitungan modal sosial yang didapatkan dengan nilai tertinggi sebesar modal sumber daya alam sebesar (3,9), modal sosial (3,0), modal finansial (3,2), modal fisik merupakan modal yang terendah dengan nilai (2,6).
Maturation of Female Yellow Rasbora (Rasbora Lateristriata Bleeker, 1854) Using Oodev at Different Doses in Feed Rey, Juniman; Widiyanto, Slamet; Retnoaji, Bambang
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.75916

Abstract

The current high demand for Yellow rasbora (Rasbora lateristriata) is not supported by the availability of captured Yellow rasbora in nature. Aquaculture is the most rational way of utilizing biological natural resources. In intensive aquaculture, it is necessary to optimize all processes that occur in aquaculture, including hatchery. However, the common problem that often happens in hatchery activities is spawning which depends on the season. The hormonal manipulation technique is an appropriate way to stimulate gonadal maturation. Oodev is a hormonal combination of pregnant mare serum gonadotropin and anti-dopamine to stimulate gonadal maturation. The purpose of this study was to determine the effectiveness of using the Oodev with different doses in feed to accelerate gonad maturation of female Yellow rasbora. The study was carried out with four treatments and three replications in 21 days with different doses of Oodev, such as; A (Feed without Oodev), B (0.5 mL/kg feed), C (1 mL/kg feed) and D (2 mL/kg feed). The parameters observed in this study were gonad maturity level, histological structure of ovary, gonadosomatic index, fecundity, and diameter of eggs. The results showed that the dose of Oodev at 1.0 mL/kg feed was an effective dose to optimize the gonad maturity of female Yellow rasbora. This is proven by the highest results shown on all parameters, such as; the maturity level in the IV phase, histological structure of the ovary which showed the dominance of the oocyte maturation phase, gonadosomatic index of 14.014%, the fecundity of 721 eggs, and egg diameter of 0.865 mm. In conclusion, using Oodev in feed at a dose of 1.0 ml/kg of feed for 21 days is an effective dose to optimize the maturation of female Yellow rasbora. 
Sistem Memori dan Pembelajaran pada Mamalia susilowati, ari; Widiyanto, Slamet; Kusindarta, Dwi Liliek; Wijayanti, Nastiti
Berkala Ilmiah Biologi Vol 15 No 3 (2024)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v15i3.17604

Abstract

Memory formation in mammals involves complex stages, from initial learning to long-term storage. This review highlights key memory-related genes and proteins such as SYNGAP1, Arc/Arg3.1, BDNF, FOXP2, COMT, NR3C1, KIBRA, H-Ras, ERK1/2, and other genes. Mammals serve as important models in memory research due to their evolutionary proximity to humans, providing insights into brain structures such as the hippocampus and prefrontal cortex, which are crucial in memory processes. Molecular mechanisms such as transcription, translation, synaptic plasticity, long-term potentiation (LTP), and long-term depression (LTD), as well as neurotransmitters like glutamate, GABA, acetylcholine, dopamine, and serotonin, are comprehensively discussed with a focus on small, large mammals, and humans. Neurotransmitters affect various functions, including emotion, cognition, memory, learning, and motor functions in test animals. Disruptions in neurotransmitter homeostasis have been linked to numerous neurological and neurodegenerative disorders, highlighting the need to understand the complex mechanisms behind memory in mammals. A brief overview of genes and neurotransmitters related to memory and learning will provide insights and considerations in basic neurobiology and biomedical research.
Histopathological Effects of Mangosteen (Garcinia mangostana L.) Peel Decoction on Betta Fish (Betta sp.) Liver Ariesti, Wiwin; Aeniah, Siti; Halim, Shuha Ma’muriyah; Sofyantoro, Fajar; Wijayanti, Nastiti; Retnoaji, Bambang; Nuriliani, Ardaning; Saragih, Hendry T.S.S.G.; Rohmah, Zuliyati; Widiyanto, Slamet; Pusparini, Nur Ainun Oktavia; Empra, Desi Eka Putri; Septriani, Nur Indah
Biota Vol 17 No 2 (2024)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mangosteen (Garcinia mangostana L.) peel contains bioactive compounds known for their health benefits, yet potential toxicity at certain doses remains a concern. This study evaluates the histopathological effects of mangosteen peel decoction on the liver of Betta fish (Betta sp.), a sensitive model organism. Mangosteen peel decoction was prepared and administered to Betta fish at concentrations of 5, 25, and 50 ppm, with a control group receiving no treatment. Fish were observed for changes in swimming activity and appetite over five days. Liver tissues were collected, processed, and analyzed histologically to assess tissue damage including vacuolization, pyknosis, hemorrhage, and necrosis. Data were analyzed using the Kruskal-Wallis and Mann-Whitney tests. Behavioral analysis indicated a dose-dependent reduction in swimming activity and appetite in treated groups. Histopathological examination revealed significant liver damage across all treatment groups, with higher concentrations of decoction correlating with increased hemorrhage, pyknosis, and necrosis. Vacuolization was highest in the control group and lowest in the 50-ppm group. The overall hepatic damage was categorized as moderate, with the control group showing the least damage. Mangosteen peel decoction induced significant hepatic damage in Betta fish, highlighting the cytotoxic effects at higher doses. The observed behavioral and histopathological changes underscore the need for careful consideration of decoction concentrations to avoid adverse effects in aquatic organisms. This study provides crucial insights into the toxicological impacts of mangosteen peel decoction on fish liver health, emphasizing the importance of dose regulation in practical applications. Further research is recommended to explore protective measures and alternative treatments to mitigate liver damage.
n-Hexane Fraction of Cucumis melo L. Cultivar Gama Melon Parfum: An in vitro Study in MCF7 and T47D Cells Line Salamah, Rohmi; Wijayanti, Nastiti; Widiyanto, Slamet
Molecular and Cellular Biomedical Sciences Vol 9, No 2 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i2.558

Abstract

Background: Cucumis melo a melon species, typically has a sweet taste. Some cultivars are known for their distinctive bitter flesh due to its higher levels of cucurbitacin. Cucurbitacin is semipolar compound that has anticancer properties. However, the anticancer effects of cucurbitacin from gama melon parfum (GMP) have not been widely studied. The use of n-Hexane as a non-polar solvent in GMP melon fractionation is to dissolve the non-polar parts of the plant. However, Cucurbitacin was found in the n-hexane fraction of Cucurbita pepo L. Therefore, this study will investigate the presence of Cucurbitacin in the n-Hexane fraction and its effects on breast cancer cells T47D and MCF7.Materials and methods: Dry simplicia of GMP melon fruit were macerated using methanol and fractionated using n-hexane. The presence of cucurbitacin was detected using the high-performance liquid chromatography (HPLC) method. Cell cytotoxicity tests were assessed using the MTT assay, with concentrations of 7.8125, 15.625, 31.25, 62.5, and 125 µg/mLResults: Cucurbitacin compounds were detected in the n-hexane fraction at a concentration of 7.6 µg/mL per 10 mg of n-hexane fraction. MCF7 cell viability was lower than that of T47D cells across all concentrations tested. MCF7 cell viability was below 50% at a concentration of 62.5 µg/mL. In contrast, T47D cell viability remained at 100% even at the highest concentration of 125 µg/mL. The IC50 value of MCF7 cells was 43.5 µg/mL.Conclusion: The cucurbitacin content in the n-Hexane fraction was 7.6 µg/mL per 10mg fraction. At this concentration, it moderately inhibits the proliferation of MCF7 cells.Keywords: gama melon parfum, cucurbitacin, HPLC, T47D, MCF7
Co-Authors Aeniah, Siti Amelia Hana Anita Yuliana Aprilia Rahmawati Ardaning Nuriliani ARI SUSILOWATI Ariesti, Wiwin Bambang Retnoaji BUDI SETIADI DARYONO Claude Mona Airin Dwi Liliek Kusindarta Eko Agus Suyono Empra, Desi Eka Putri Fajar Sofyantoro Giriani Ayu Sabilla Halim, Shuha Ma’muriyah Hendry T.S.S.G. Saragih Hendry Tri Sakti Saragih HERA MAHESHWARI I Wayan Swarautama Mahardhika Irkham Widiyono Laksmindra Fitria LUTHFIRALDA SJAHFIRDI Muhamad Muhammad Muhammad Mulyati Mulyati Mulyati Mulyati Mulyati Sarto Nastiti Wijayanti Prabowo Purwono Putro Pudji Astuti PUDJI ASTUTI Pusparini, Nur Ainun Oktavia Rahadian Yudo Rey, Juniman Rohmah, Zuliyati Salamah, Rohmi Sarmin Sarmin Septriani, Nur Indah Syamsul Maarif