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Efek Samping Lama Aplikasi dari Bleaching Agent Hidrogen Peroksida 40% Terhadap Kekasaran Permukaan Resin Komposit Tipe Nanofill Izfan Yulviansyah; Widjijono Widjijono; Dyah Irnawati
Jurnal Material Kedokteran Gigi Vol 2 No 2 (2013): JMKG Vol 2 No 2 September 2013
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.726 KB)

Bleaching is a dental treatment chemically on the vital and non vital teeth undergoing discoloration. One technique is in office bleaching. Application of bleaching agent is not only touching the healthy tooth tissue, but also potentially touched restorative nanofill dental composite resins. Materials research is resin composite nanofil (Filtek Z350, 3 m, USA) and the bleaching agent 40% hydrogen peroxide (Opalescense Boost, USA). Disc-shaped sample (diameter 10 mm, thickness 2 mm) as many as 12 samples and divided into 3 groups each sample 4. The samples applied with bleaching agent 40% hydrogen peroxide at room temperatures (25 C) and contact time: 30 minutes, 45 minutes, and 60 minutes. Surface roughness was measured before and after application of bleaching. Surface roughness was measured before and after application of bleaching. Statistical analysis using a one way ANOVA and LSD 0,05. The results showed the average delta composite resin nanofil roughness with time sequential contacts 30, 45 and 60 minutes is 0,040 0,016 µm ± 0,045 µm ± 0.025, and 0.025 ± 0.125 µm. ANOVA test showed that there is a sinificant influence of variation of long application of hydrogen peroxide 40% against a nanofill composite resin surface roughness (p< 0.05). Test LSD indicates there is a significant difference between the delta’s average surface roughness on all groups, except the group of 30 and 45 minutes. Conclusion this research is a variation time contacts of 40% hydrogen peroxide application increases the surface roughness of a composite resin nanofil.

Perbandingan Kekuatan Tarik antara Stranded Fiber dengan Braided Fiber pada Fiber Reinforced Composite Jenis Ultra High Molecular Weight Polyethylene (UHMWPE) Prihashinta Uswatun Hasanah; Purwanto Agustiono; Widjijono Widjijono
Jurnal Material Kedokteran Gigi Vol 3 No 1 (2014): JMKG Vol 3 No 1 Maret 2014
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

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Fiber reinforced composite (FRC) is a material that has at least two components were matrix and fiber. Difference of fiber architecture on the FRC may affect the mechanical strength of FRC. Stranded and braided fiber fiber is one of materials that are commonly used in dentistry, both have differences from the sides of the fiber arcitecture. This research aims to know the comparative tensile strength between fiber stranded with braided fiber on FRC type of UHMWPE. This research uses 8 samples were divided into 2 groups. The types of UHMWPE fiber with braided fiber (Construct TM, Kerr, USA) and stranded fiber (Biodental TM, Australia). The sample is 20x10x2mm according ASTM 638M. Tensile strength is measured by using a universal testing machine. Tensile strength data then tested using the indepedent t-test. The results showed the tensile strength fiber and fiber braided stranded i.e. of 35.65 ± 2.538 MPa and 49.43 ± 2.953 MPa. Independent t-test results test shows there is difference significantly in tensile strength between fiber and fiber braided stranded (p < 0.05). The conclusion of the research was there stranded fiber tensile strength higher than with braided fiber on FRC type of UHMWPE.

Pengaruh Perendaman Pada Saliva Buatan Selama 14 Hari Pada Glass Fiber Non Dental Dan Glass Fiber Dental Terhadap Perlekatan Bakteri Streptococcus Mutans Zwista Yulia Dewi; Widjijono Widjijono; Erna Prawita Setyowati
Jurnal Material Kedokteran Gigi Vol 6 No 1 (2017): JMKG Vol 6 No 1 Maret 2017
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

The material in the oral cavity will be coated by saliva. The humidity of material that is immersed in water will increase from time to time. Longer immersion time in water causes an increase in the absorption of water by a material. Non-dental glass fiber can be used as an alternative replacement for fiber glass dental. The advantages of dental glass fiberis widely available with reasonably priced. Fiber glass non dental commonly used as building materials and automotive. Streptococcus mutans bacteria have the ability to stick to the whole surface of the mouth, including dental materials. The purpose of this study is to determine the effect of dental glass fiber and non-dental glass fiber immersion in artificial saliva for 14 days to the adhesions of Streptococcus mutans bacteria.Fiber used in this study is E-glass fiber dental(Fibersplint, Polydentia SA, Switzerland), non-dental glass fiber A (LT, China), flowable composite (CharmFil Flow, Denkist, Korea) and silane coupling agent (Monobond S, Ivoclar Vivadent, Liechtenstein). Subjects were divided into three groups, each group consisting of 5 samples. Streptococcus mutans bacteria was planted in BHI media and calculated by plate count method. Results were analyzed using ANAVA. From the research it showed that the average dental glass fiber bacterial adhesions value was (953.4 ± 7.19), non-dental glass fiber(953.4 ± 7.19) while the composite resin without using fiber glass (848 ± 7.07). Statistical analysis showed a variable composition have a significant effect (p <0.05). The conclusion of this research that the alkali metal content in the fiber glass can affect the amount of bacteria Streptococcus mutans adhesions.

Perbandingan Hipersensitivitas Tipe IV Akibat Paparan Remanium Gm800 Dan Stainless Steel 316L Ikmal Hafizi; Widjijono Widjijono; Marsetyawan HNE Soesatyo
Jurnal Material Kedokteran Gigi Vol 6 No 1 (2017): JMKG Vol 6 No 1 Maret 2017
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jmkg.v6i1.264

Stainless steel and cobalt chromium is a metal that is used in dentistry. Stainless steel (SS) 316L has good corrosion resistance, but there are still many cases of hypersensitivity due to the use of such materials. Remanium GM800 is a cobalt-based alloy which is relatively mild with the advantages of having a high fracture resistance, high modulus of elasticity and resistance to corrosion. The research aims to know type IV hypersensitivity reactions for cobalt chromium GM800 applications compared with 316L Stainless steel. The research was conducted through the test GPMT (Guinea Pig Maximization Test). The pre-research phase does CoCr patch/SS/control application to 3 guinea pigs of each group with concentrations of 5%, 10%, 20%, 40%, and 80%. Primary research begins with intradermal induction on the backs of guinea pigs for 7 days with a suspension of A (50% FCA emulsion dan 50% Propylene glycol), B (SS/CoCr/blank dan Propylene glycol) and C (50% SS/CoCr/blank suspension and 50% FCA) on the left and right backs of guinea pigs. On the 8th day induction results topical concentration of 40% for 24 hours, then opened to see the reaction and closed again for 48 hours. After that, the research was continued with challenge phase by attaching patch 5% concentration for 14 days. On the 28th day was observed erythema and edema on the skin of guinea pigs followed by sacrifice in order to obtain specimen to do immunohistochemical staining by ED antibodies. The result showed 316L SS cause 40% of the samples sensitized that were grouped in moderate classification, while CoCr GM800 cause 20% of the samples sensitized so classified in the mild classification in triggering type IV hypersensitivity reaction. Histopathology examination showed that 42% of the visual field SS 316L specimen expressed macrophages, while only 28% expressed macrophages in CoCr GM800 specimen. The conclusion of this study CoCr GM800 trigger type IV hypersensitivity reaction is lower than SS 316L.

Enhancing Compressive Strength and Dentin Interaction of Mineral Trioxide Aggregate by Adding SrO and Hydroxyapatite Leny Yuliatun; Eko Sri Kunarti; Widjijono Widjijono; Nuryono Nuryono
Indonesian Journal of Chemistry Vol 22, No 6 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.76231

In this research, the effect of strontium oxide (SrO) and hydroxyapatite (HA) on the properties of mineral trioxide aggregate (MTA) have been studied. MTA contained 20% SiO2, 60% CaO, and 2% Al2O3. Bi2O3 and SrO have been added with 18% (w/w) total percentage. MTA was prepared with a sol-gel process using a weak base (NH3) as a catalyst and calcined at 1000 °C for 3 h. The effect of HA was investigated by adding various percentages (3, 6, and 9%) on the MTA modified with 5% SrO. The modified MTA (MTA-SrO-HA) products were hydrated using water with the MTA to water weight ratio of 3:1. The results showed that tricalcium silicate (C3S), dicalcium silicate (C2S), Bi2O3, and strontium silicate peaks were detected in the XRD patterns. An increase in the intensity in the infrared spectra of CaO occurred after hybridization with HA. In addition, bonding of Ca-O-Si appeared at 879 and 995 cm−1, indicating the formation of cement. MTA modified with 5% SrO and 6% HA showed similar compressive strength to the commercial MTA (ProRoot brand). Furthermore, MTA-SrO5/HA6 showed a strong interface interaction with dentin adheres without any gaps, indicating a potential dental material for the future.

The effect of casein and lactoferrin of bovine's milk on nestin expression of the exposed dental pulp Endah Ariyati Eko Ningtyas; Widjijono Widjijono; Siti Sunarintyas
Odonto : Dental Journal Vol 9, No 2 (2022): December 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.2.273-278

Background: Pulp tissue can regenerate if damage occurs. The pulp differentiates into new odontoblast-like cells in Progenitor cells. Nestin is a marker of the presence of new dontoblast-like and expressed in pathological conditions. Bovine's milk casein and lactoferrin contain proteins that accelerate cell growth. This study aimed to determine the effect of casein and lactoferrin on the expression of nested cells in the exposed dental pulp.Method: The research method is an experimental laboratory with male 30 Spraque Dawley rats. Rats were grouped as : casein, lactoferrin, casein-lactoferrin, and Ca-(OH)2. Each group consisted of 15 samples of molars which were divided into 5 samples of teeth for observation on the 7, 14, and 21 days. The first molars of rats were prepared until the pulp was exposed. The data of nestin quantity with immunohistochemical (IHC) analysis on histological preparations were analyzed using two-way ANOVA then the least significance difference (LSD) test.Result: The results of the average number of nested cells on 7,14 and 21 days were highest on combination of casein and lactoferrin. Two-way ANOVA statistical proved that there was a significant difference (p<0.05) in each observation period and group and the number of expressions of nestin cells. LSD test showed that there was a significant difference (p<0.05) between groups compared to the control group.Conclusion: Combination casein and lactoferrin extracts affect the expression of the number of nestin cells as a marker of new odontoblast-like cells in the exposed dental pulp.

Penyerapan Air Glass Fiber Non Dental – Fiber Reinforced Composite dalam Larutan Mouthwash Etny Dyah Harniati; Widjijono Widjijono; Budiono Budiono
Jurnal Material Kedokteran Gigi Vol 10 No 2 (2021): JMKG Vol 10 No 2 September 2021
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jmkg.v10i2.452

Fiber reinforced composite (FRC) is widely used to replace porcelain fused to metal (PFM) for patients who have lost teeth. Fiber reinforced composite is resin matrix protheses with fiber reinforced to get optimal strength. Glass fiber dental is often used because it does not require complex preparations, not sensitive to chemicals and moisture, but it has an expensive price for Indonesian people and limited availability. An alternative material such as glass fiber non dental can used. Mouthwash is used as an antiseptic and anti-plaque material on the oral cavity. Mouthwah can affect of water sorption of FRC. The aim of this study is to know effect of mouthwash on the absorption of water in non dental glass fiber - reinforced FRC. Sixty specimens divided into four groups (n=4), 2 groups with glass fiber dental soaked on the bisguanida and aquades, 2 groups with glass fiber non dental soaked on the bisguanida and quaternary ammonium. The data analyzed with Anova. Anova test showed that there is an influence of mouthwash bisguanide and quartenary ammonium compounds on the value of absorption of glass fiber reinforced composite water (p<0,05). Water sorption of FRC in bisguanide mouthwash is higher than quartenary ammonium compounds.

The effect of casein and lactoferrin of bovine's milk on nestin expression of the exposed dental pulp Endah Ariyati Eko Ningtyas; Widjijono Widjijono; Siti Sunarintyas
Odonto : Dental Journal Vol 9, No 2 (2022): December 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Pulp tissue can regenerate if damage occurs. The pulp differentiates into new odontoblast-like cells in Progenitor cells. Nestin is a marker of the presence of new dontoblast-like and expressed in pathological conditions. Bovine's milk casein and lactoferrin contain proteins that accelerate cell growth. This study aimed to determine the effect of casein and lactoferrin on the expression of nested cells in the exposed dental pulp.Method: The research method is an experimental laboratory with male 30 Spraque Dawley rats. Rats were grouped as : casein, lactoferrin, casein-lactoferrin, and Ca-(OH)2. Each group consisted of 15 samples of molars which were divided into 5 samples of teeth for observation on the 7, 14, and 21 days. The first molars of rats were prepared until the pulp was exposed. The data of nestin quantity with immunohistochemical (IHC) analysis on histological preparations were analyzed using two-way ANOVA then the least significance difference (LSD) test.Result: The results of the average number of nested cells on 7,14 and 21 days were highest on combination of casein and lactoferrin. Two-way ANOVA statistical proved that there was a significant difference (p<0.05) in each observation period and group and the number of expressions of nestin cells. LSD test showed that there was a significant difference (p<0.05) between groups compared to the control group.Conclusion: Combination casein and lactoferrin extracts affect the expression of the number of nestin cells as a marker of new odontoblast-like cells in the exposed dental pulp.

Orientation and Type of Non-dental Glass Fiber Towards The Flexural of Fiber Reinforced Composite Etny Dyah Harniati; Widjijono Widjijono; Rini Dharmastiti
Indonesian Journal of Dentistry Vol 1, No 1 (2021): February 2021
Publisher : Universitas Muhammadiyah Semarang

Introduction : Non-dental glass fiber can used as alternative dental glass fiber. Flexural strength to withstand masticatory loads that influenced by various factors, including the orientation and type of fiber used. Purpose : The purpose of this study was to see the effect of orientation and type of non-dental glass fiber on flexural strength. Method : The research method used FRC samples measuring 65 x 10 x 2,5 mm which were reinforced with non-dental glass fiber mats, roving, woven roving, and dental glass fiber roving. Non-dental glass fiber mats and woven roving was cut according to the length and width of the sample, while the non-dental glass fiber roving was weighed according to the weigh tof 4 sheets of dental glass fiber roving to meet the sample mold zone. Result : Glass fiber placed in the tension position in the sample mold. The flexural strength was tested using universal testing machine. This study indicate that the type of glass fiber doesn’t have a significant effect on the flexural strength of FRC with reinforced non dental glass fiber and dental glass fiber. Conclusion : The orientation of non-dental glass fiber roving as reinforcement of FRC has greater flexural strength than non-dental glass fiber with mats and woven roving orientation.

PENGARUH JENIS DAN LAMA PERENDAMAN NON DENTAL GLASS FIBER REINFORCED COMPOSITE TERHADAP SITOTOKSISITAS SEL FIBROBLAS Dendy Murdiyanto; Widjijono Widjijono; Nuryono Nuryono
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Volume 2, Nomor 1, Juni 2015
Publisher : Universitas Baiturrahmah

Perawatan di kedokteran gigi mulai menggunakan material fiber reinforced composite (FRC) sebagai bahan penyusun alat-alat tertentu seperti gigi tiruan cekat, restorasi onlay, splinting gigi goyah, pasak gigi dan space maintainer. Penyusun FRC terdiri dari fiber dengan jenis terbanyak glass fiber dan matriks berupa dental composite. Non dental glass fiber merupakan jenis glass fiber yang digunakan pada pembuatan gypsum, patung dan alat-alat otomotif yang mudah dijumpai di pasaran dengan harga terjangkau. Tujuan penelitian ini adalah untuk mengetahui potensi sitotoksisitas jenis dan lama perendaman non dental glass fiber reinforced composite terhadap sel fibroblas yang mati. Penelitian ini menggunakan FRC yang diperkuat oleh 3 jenis non dental glass fiber I, II, III dan dental E-glass fiber sebagai pembanding. Uji sitotoksisitas dilakukan dengan methyl tetrazolium test (MTT) menggunakan sel vero terhadap air hasil rendaman FRC selama 1, 4, 7 dan 10 hari masing-masing 6 pengulangan sampel tiap kelompok. Jumlah sel yang mati menunjukkan tingkat sitotoksisitas dan kemudian dianalisa dengan Anava dua jalur (α = 0,05).Hasil penelitian menunjukkan rata-rata kematian sel tertinggi yaitu 8,55 ± 0,27 % pada FRC III dengan lama perendaman 10 hari, sedangkan rata-rata kematian sel terendah yaitu 8,48 ± 0,35 % pada dental glass fiber dengan lama perendaman 1 hari. Berdasarkan pedoman dari Sjögren bahan tidak bersifat sitotoksis jika kematian sel masih dibawah 10%. Uji Anava dua jalur menunjukkan bahwa jenis non dental glass fiber reinforced composite dan lama perendaman mempunyai pengaruh tidak bermakna (p>0,05) terhadap sitotoksisitas sel fibroblas. Kesimpulan hasil penelitian yaitu non dental glass fiber reinforced composite tidak bersifat sitotoksis terhadap sel fibroblas, jenis non dental glass fiber reinforced composite dan lama perendaman tidak berpengaruh terhadap sitotoksisitas sel fibroblas

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