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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences ENVIAGRO Jurnal Sumberdaya Hayati Biopropal Industri BERITA BIOLOGI Jurnal Penelitian Karet ANNALES BOGORIENSES Makara Journal of Science Annales Bogorienses
UTUT WIDYASTUTI
Institut Pertanian Bogor, Jl. Raya Dramaga, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology

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The Effects of Root Endophytic and Arbuscular Mycorrhizal Fungi on Growth and Curcumin Content of Temulawak (Curcuma xanthorrhiza) : The Effects of Root Endophytic and Arbuscular Mycorrhizal Fungi on Growth and Curcumin Content of Temulawak (Curcuma xanthorrhiza) Putra, Sukma Triperdana; Iswantini, Dyah; Widyastuti, Utut; Fadillah, Wendi Nurul; Sukarno, Nampiah
Jurnal Sumberdaya Hayati Vol. 8 No. 2 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.2.49-56

Abstract

Temulawak (Curcuma xanthorrhiza) is known to be used as ingredient in Jamu and traditionally utilized to cure a range of illness. The global market demand for the rhizome of the temulawak affecting the urgency of sustainable rizhome production. The aim of this research was to analyze the effects of root endophytic and arbuscular mycorrhizal fungi on growth and rhizome curcumin content of the temulawak. There were five fungal inoculation treatments on C. xanthorrhiza seedling grown in sterilized and unsterilized growth media. They were root endophytic fungi A. niger (A), Glomus sp. (G), combination of A. niger and AMF Glomus sp. applied at the same time (GA) and at different time (G-A) and control. The experiment was conducted under greenhouse condition. The plant growth parameters, fungal colonization, and rhizomes curcumin content were measured. The result showed that there was a significant interaction between fungal inoculation and control treatments. In general, fungal inoculation on both sterilized and unsterilized growth media increased the plant growth and rhizomes curcumin content. On the sterilized growth media, C. xanthorrhiza inoculated by A. niger showed the best growth parameter. On the other hand, the best growth parameter on the unsterilized media was showed by C. xanthorrhiza inoculated by mixed fungal inoculums. The highest amount of curcumin content was showed by C. xanthorrhiza inoculated by Glomus sp. as a single inoculation.
Keragaman Genetik Kelapa Sawit (Elaeis guineensis Jacq.) Asal Angola Menggunakan Marka SSR Sayekti, Urip; Widyastuti, Utut; Toruan-Mathius, Nurita
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.461 KB) | DOI: 10.24831/jai.v43i2.10420

Abstract

ABSTRACTEffort to increase productivity and other elite characters in Indonesia oil palm breeding program is facing a problem because of the narrow genetic diversity. To broaden the genetic diversity, germplasm exploration has been done in Angola, Central Africa. The objective of this research was to assess the genetic diversity and population structure of Angola originated oil palm germplasm based on 20 SSR markers. The plant materials used were 27 accessions consisted of 136 palms planted in Riau, Sumatera. The DNA was isolated and amplified using PCR. Phylogeny analysis was constructed using Unrooted Neighbor-Joining by DARwin software 6.0.8. The result showed that polymorphic information content (PIC) value is 0.55 (0.17 to 0.75 for each locus) with 102 total number of alleles. Genetic diversity between individuals was higher compared to the genetic diversity within accessions or regions and between accessions or regions. Phylogenetic analysis of 27 accessions showed that accessions were divided into three main groups. Every group containing individuals originated from 5 spatial distribution regions. Principal coordinates analysis (PCoA) showed that accessions were distributed in one structure. Using more primers and samples to get more representative data is recommended for the following research.Keywords: allele, locus, germplasm, molecular marker, polymorphic
Optimization of Somatic Embryogenesis Induction of Cassava (Manihot esculenta Crantz) Susanti, Idha; Suharsono, Suharsono; Widyastuti, Utut; Siregar, Ulfah Juniarti; Tjahjoleksono, Aris
Annales Bogorienses Vol. 21 No. 2 (2017): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The embryogenesis (SE) has important role for genetic engineering of cassava (Manihot esculenta Crantz). However, the success of SE induction depend on plant growth regulator s (PGR)s and treatment enriched in induction media. This experiment tried to induce callus formation of cassava from several in vitro explants: immature leaf, apical bud, and internode; and to develop somatic embryogenesis of cassava in several media enriched with tyrosine and copper sulphate (CuSO4) added into media enrich with picloram as treatment. Different response of explants source to callus induction treatment from those three varieties in callus induction as well as friable callus formation were found in this experiment. The best medium to induce varied with variety; MS media supplemented 12 mg/L picloram + 0.5 mg/L CuSO4 was the best for “Adira 4” and half MS and half GD media supplemented 12 mg/L picloram + 100 mg/L tyrosine for “Malang 6”. All treatments resulted somatic embryo which developed indirectly and in morphologically normal somatic embryos
Co-Authors . Syafarudin, . AKIHO YOKOTA ALEX HARTANA Ardiwinata Ardiwinata, Ardiwinata Aris Tjahjoleksono Asep Nugraha, Asep Asri Pirade Paserang Didy Sopandie DWI SUSILANINGSIH DWINITA WIKAN UTAMI Dyah Iswantini Eries Dyah Mustikarini Fadillah, Wendi Nurul Hadi Susilo Hamim Hamim Hani Susanti, Hani Ida Rosdianti, Ida Idha Susanti Indradewa, Rhian KINYA AKASHI Nampiah Sukarno Nurita Toruan-Mathius Nurwijayanti Ochieng, Fredrick Onyango Putra, Sukma Triperdana RADITE TISTAMA ROHANI CINTA BADIA GINTING Suharsono Suharsono Suharsono, Sukmarayu P. Gedoan Sulistyaningsih, Yohana C Swastika, I Nengah Tiwit Widowati, Tiwit Ulfah Juniarti Siregar Umoro, Asahedi Urip Sayekti Widyaningrum, Dian Noverita windarsih, gut Yanti Rosa, Yanti Yuniati, Ratna