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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences BIOTROPIA - The Southeast Asian Journal of Tropical Biology ENVIAGRO Jurnal Sumberdaya Hayati Biopropal Industri BERITA BIOLOGI Jurnal Penelitian Karet ANNALES BOGORIENSES Makara Journal of Science Annales Bogorienses
UTUT WIDYASTUTI
Institut Pertanian Bogor, Jl. Raya Dramaga, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Veterinary

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The Effects of Root Endophytic and Arbuscular Mycorrhizal Fungi on Growth and Curcumin Content of Temulawak (Curcuma xanthorrhiza) : The Effects of Root Endophytic and Arbuscular Mycorrhizal Fungi on Growth and Curcumin Content of Temulawak (Curcuma xanthorrhiza) Putra, Sukma Triperdana; Iswantini, Dyah; Widyastuti, Utut; Fadillah, Wendi Nurul; Sukarno, Nampiah
Jurnal Sumberdaya Hayati Vol. 8 No. 2 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.2.49-56

Abstract

Temulawak (Curcuma xanthorrhiza) is known to be used as ingredient in Jamu and traditionally utilized to cure a range of illness. The global market demand for the rhizome of the temulawak affecting the urgency of sustainable rizhome production. The aim of this research was to analyze the effects of root endophytic and arbuscular mycorrhizal fungi on growth and rhizome curcumin content of the temulawak. There were five fungal inoculation treatments on C. xanthorrhiza seedling grown in sterilized and unsterilized growth media. They were root endophytic fungi A. niger (A), Glomus sp. (G), combination of A. niger and AMF Glomus sp. applied at the same time (GA) and at different time (G-A) and control. The experiment was conducted under greenhouse condition. The plant growth parameters, fungal colonization, and rhizomes curcumin content were measured. The result showed that there was a significant interaction between fungal inoculation and control treatments. In general, fungal inoculation on both sterilized and unsterilized growth media increased the plant growth and rhizomes curcumin content. On the sterilized growth media, C. xanthorrhiza inoculated by A. niger showed the best growth parameter. On the other hand, the best growth parameter on the unsterilized media was showed by C. xanthorrhiza inoculated by mixed fungal inoculums. The highest amount of curcumin content was showed by C. xanthorrhiza inoculated by Glomus sp. as a single inoculation.
Keragaman Genetik Kelapa Sawit (Elaeis guineensis Jacq.) Asal Angola Menggunakan Marka SSR Sayekti, Urip; Widyastuti, Utut; Toruan-Mathius, Nurita
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.461 KB) | DOI: 10.24831/jai.v43i2.10420

Abstract

ABSTRACTEffort to increase productivity and other elite characters in Indonesia oil palm breeding program is facing a problem because of the narrow genetic diversity. To broaden the genetic diversity, germplasm exploration has been done in Angola, Central Africa. The objective of this research was to assess the genetic diversity and population structure of Angola originated oil palm germplasm based on 20 SSR markers. The plant materials used were 27 accessions consisted of 136 palms planted in Riau, Sumatera. The DNA was isolated and amplified using PCR. Phylogeny analysis was constructed using Unrooted Neighbor-Joining by DARwin software 6.0.8. The result showed that polymorphic information content (PIC) value is 0.55 (0.17 to 0.75 for each locus) with 102 total number of alleles. Genetic diversity between individuals was higher compared to the genetic diversity within accessions or regions and between accessions or regions. Phylogenetic analysis of 27 accessions showed that accessions were divided into three main groups. Every group containing individuals originated from 5 spatial distribution regions. Principal coordinates analysis (PCoA) showed that accessions were distributed in one structure. Using more primers and samples to get more representative data is recommended for the following research.Keywords: allele, locus, germplasm, molecular marker, polymorphic
ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE SUHARSONO, SUHARSONO; TRISNANINGRUM, NIKEN; SULISTYANINGSIH, LULUT DWI; WIDYASTUTI, UTUT
BIOTROPIA Vol. 16 No. 1 (2009): BIOTROPIA Vol. 16 No. 1 June 2009
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.362 KB) | DOI: 10.11598/btb.2009.16.1.64

Abstract

Metallothionein is an important protein for detoxifying heavy metal ions. This research was conducted to isolate and clone cDNA of gene encoding for metallothionein type 2 from Melastoma affine. Total RNA was isolated from young leaves. Total cDNA was synthesized from the total RNA by reverse transcription. The MaMt2 cDNA was successfully isolated by PCR technique. The MaMt2 cDNA was inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into Escherichia coli DH5α. DNA sequencing analysis showed that this cDNA is full length consisting of 246 pb encoding 81 amino acid residues. This cDNA is identical to mRNA of AtMt2 from Arabidopsis thaliana. It does not contain any restriction sites found in the cloning sites of pGEM-T easy. The deduced protein of MaMT2 contains 14 cysteine residues distributed in the Cys-Cys, Cys-X-Cys, and Cys-X-X-Cys motifs.   Key words: cDNA, metallothionein, Melastoma affine, cloning, cysteine
Optimization of Somatic Embryogenesis Induction of Cassava (Manihot esculenta Crantz) Susanti, Idha; Suharsono, Suharsono; Widyastuti, Utut; Siregar, Ulfah Juniarti; Tjahjoleksono, Aris
Annales Bogorienses Vol. 21 No. 2 (2017): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The embryogenesis (SE) has important role for genetic engineering of cassava (Manihot esculenta Crantz). However, the success of SE induction depend on plant growth regulator s (PGR)s and treatment enriched in induction media. This experiment tried to induce callus formation of cassava from several in vitro explants: immature leaf, apical bud, and internode; and to develop somatic embryogenesis of cassava in several media enriched with tyrosine and copper sulphate (CuSO4) added into media enrich with picloram as treatment. Different response of explants source to callus induction treatment from those three varieties in callus induction as well as friable callus formation were found in this experiment. The best medium to induce varied with variety; MS media supplemented 12 mg/L picloram + 0.5 mg/L CuSO4 was the best for “Adira 4” and half MS and half GD media supplemented 12 mg/L picloram + 100 mg/L tyrosine for “Malang 6”. All treatments resulted somatic embryo which developed indirectly and in morphologically normal somatic embryos
cDNA ENCODING GROWTH HORMONE FROM HUMPBACK GROUPER (Cromileptes altivelis) Syaifudin, Mochamad; Alimuddin; Widyastuti, Utut; Sudrajat, Agus Oman; Sumantadinata, Komar; Aliah, Ratu Siti
BIOTROPIA Vol. 14 No. 2 (2007): BIOTROPIA Vol. 14 No. 2 December 2007
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2007.14.2.14

Abstract

Growth hormone (GH) that plays an important role in growth, reproduction, seawater adaptation, and immune function was isolated and sequenced from humpback grouper, Cromileptes altivelis. The cDNA was isolated from pituitary using RT-PCR. The 618 bp open reading frame encodes a 205 amino acid (aa) protein, which represents an 18 aa signal peptide followed by a 187 aa mature GH polypeptide. The fragment contained conserved domain of somatotropin–1, somatotropin–2, casein kinase II phosphorylation, protein kinase C phosphorylation, N–myristoylation and N–glycosilation. The similarity of deduced protein of humpback grouper GH was 65.0 - 89.5% with other fishes
Introduction of the Serine Green Fluorescent Protein (sGFP) Gene into Pyricularia grisea Race dc4 Isolated from Digitaria ciliaris using Agrobacterium tumefaciens-mediated Genetic Transformation Stephanie, Stephanie; Widyastuti, Utut; Wiyono, Suryo
BIOTROPIA Vol. 22 No. 1 (2015): BIOTROPIA Vol. 22 No. 1 June 2015
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2015.22.1.329

Abstract

Blast disease (caused by Pyricularia grisea) has long been known as a serious problem for upland rice, and recently, it has also begun infecting lowland rice. However, the mechanism enabling this range expansion is still unknown. One possible method to study this shift is by inserting a marker gene into P. grisea so that its spread can be monitored. The sGFP (Synthetic Green Fluorescent Protein) gene has been widely used as a reporter to track gene expression and cellular processes in fungi. In this study, the sGFP gene was integrated into the genome of P. grisea isolate DC4 from Digitaria ciliaris. The sGFP plasmid was first introduced into Agrobacterium tumefaciens using the triparental mating method (TPM). Genetic transformation was then carried out by co-cultivating spores of P. grisea DC4 with A. tumefaciens strain LBA4404 harboring the pCAMBIA-sGFP construct. Transformants were selected on medium containing 300 µg/mL hygromycin. Successful integration of the sGFP gene into the fungal genome was confirmed using PCR with sGFP-specific primers, an sGFP–Nos terminator primer pair, and a β-tubulin primer pair as an internal control. Fluorescent microscopy using blue light excitation confirmed sGFP expression in the P. grisea DC4 transformant.
Binary Vector Construction and Agrobacterium tumefaciens-mediated Transformation of Lysozyme Gene in Seaweed Kappaphycus alvarezii handayani, Tri; Alimuddin, Alimuddin; Widyastuti, Utut; Suryati, Emma; Parenrengi, Andi
BIOTROPIA Vol. 21 No. 2 (2014): BIOTROPIA Vol. 21 No. 2 December 2014
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2014.21.2.348

Abstract

Ice-ice disease is the biggest problem in the cultivation of seaweed Kappaphycus alvarezii. The disease is caused by bacterial infection and induced by drastic changes of water quality. Lysozyme has the ability to break down bacterial cell wall. The purpose of this research was to construct of a binary vector pMSH1-Lys carrying chicken Lysozyme (Lis) gene and introduce pMSH1-Lis on K. alvarezii. The binary vector expression was transformed into Agrobacterium tumefaciens LBA4404 by triparental mating. Thallus was inoculated with A. tumefaciens carrying pMSH1-Lis and then the transformed thallus was selected by adding 20 mg/L hygromycin to the culture medium. PCR analysis showed that the construction of the binary plasmid pMSH1-Lys was established. Percentage of transformation of pMSH1-Lis on K. alvarezii was 23.56%, while the efficiency of regeneration was 11.32%. PCR analysis showed that three of the regenerated thallus contained Lysozyme gene. Thus, transgenic K. alvarezii was produced successfully and this can be useful for studying the mechanisms of seaweed defense against bacterial infection.
Co-Authors . Syafarudin, . Agus Oman Sudrajat AKIHO YOKOTA ALEX HARTANA Alimuddin Andi Parenrengi Ardiwinata Ardiwinata, Ardiwinata Aris Tjahjoleksono Asep Nugraha, Asep Asri Pirade Paserang Didy Sopandie DWI SUSILANINGSIH DWINITA WIKAN UTAMI Dyah Iswantini Emma Suryati Eries Dyah Mustikarini Fadillah, Wendi Nurul Hadi Susilo Hamim Hamim Hani Susanti, Hani Ida Rosdianti, Ida Idha Susanti Indradewa, Rhian KINYA AKASHI Komar Sumantadinata Lulut Dwi Sulistyaningsih Mochamad Syaifudin, Mochamad Nampiah Sukarno Nurita Toruan-Mathius Ochieng, Fredrick Onyango PUJI LESTARI Putra, Sukma Triperdana RADITE TISTAMA Ratu Siti Aliah ROHANI CINTA BADIA GINTING Stephanie Stephanie Suharsono Suharsono Suharsono, Sukmarayu P. Gedoan Sulistyaningsih, Yohana C Suryo Wiyono Swastika, I Nengah Tiwit Widowati, Tiwit Tri Handayani Trisnaningrum, Niken Ulfah Juniarti Siregar Umoro, Asahedi Urip Sayekti Widyaningrum, Dian Noverita windarsih, gut Yanti Rosa, Yanti Yuniati, Ratna