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Journal : PHARMACON

FORMULASI DAN UJI EFEKTIVITAS ANTIBAKTERI SEDIAAN SABUN WAJAH CAIR EKSTRAK ETANOL BUAH PARE (Momordica Charantia L.) TERHADAP BAKTERI Staphylococcus Epidermidis Marhaba, Fera Anelia; Yamlean, Paulina V.Y.; Mansauda, Karlah L.R.
PHARMACON Vol 10, No 3 (2021): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.10.2021.35609

Abstract

ABSTRACTBitter melon (Momordica Charantia L.) contains flavonoid compounds, alkaloids, and saponins which have antibacterial properties. The perpose of this study was to formulate the ethanolic extract of bitter melon into liquid facial soap preparations and to test the antibacterial effectiveness of liquid facial soap preparations against bacteria Staphylococcus epidermidis. This study have done by using laboratory experimental methods. Antibacterial effectiveness of bitter melon extract liquid facial soap was tested using the well diffusion method. Based on the results of the study, it showed that physical evaluations such as organoleptic tests produced (Odor: typical apple scent, color: green, texture: liquid), pH test was 5.5, foam height measurement was 60-62 mm, specific gravity test was 1.03 -1,05 g/ml , free alkali level test was 0.05%-0.08% , water content test was 32-38%. The liquid facial soap obtained from the ethanol extract of bitter melon fruit can inhibitbacteria Staphylococcus epidermidis with strong category in concentration of 2.5% (10.16 mm); 5% (11.58 mm); 7.5% (12.5 mm); 10% (13 mm) and 12.5% (14 mm). It can be concluded that the ethanol extract bitter melon can be formulated into a liquid facial soap that has good physical quality and has the greatest inhibitory power in concentration 12.5%. Keywords: Bitter melon, Liquid Facial Soap, Evaluation of Physical Properties, Antibacterial.  ABSTRAK Buah Pare (Momordica Charantia L.) memiliki kandungan senyawa flavonoid, alkaloid, dan saponin yang berkasiat sebagai antibakteri. Penelitian yang dilakukan bertujuan untuk memformulasikan ekstrak etanol buah Pare menjadi sediaan sabun wajah cair dan menguji efektivitas antibakteri sediaan sabun wajah cair terhadap bakteri Staphylococcus epidermidis. Penelitian ini menggunakan metode eksperimental laboratorium. Pengujian efektivitas antibakteri sabun wajah cair ekstrak buah pare menggunakan metode difusi sumuran. Berdasarkan hasil penelitian, menunjukkan evaluasi fisik seperti uji organoleptik menghasilkan (Bau: khas pengaroma apel, warna: hijau, tekstur: Cair), Uji pH yaitu 5,5, Pengukuran tinggi busa yaitu 60-62 mm, uji bobot jenis yaitu 1,03-1,05 g/ml , uji kadar alkali bebas yaitu: 0,05%-0,08%, uji kadar air yaitu 32-38%. Sabun wajah cair ekstrak etanol buah Pare yang diperoleh dapat menghambat bakteri Staphylococcus epidermidis dengan kategori kuat pada konsentrasi 2,5% (10,16 mm); 5% (11,58 mm); 7,5% (12.5 mm); 10% (13 mm) dan 12,5% (14 mm). Dapat disimpulkan bahwa ekstrak etanol buah Pare dapat diformulasikan menjadi sediaan sabun wajah cair yang memiliki mutu fisik yang baik dan memliki daya hambat yang paling besar pada konsentrasi 12,5%. Kata kunci: Buah Pare, Sabun Wajah Cair, Evaluasi Sifat Fisik, Antibakteri.
FORMULASI SEDIAAN SABUN MANDI PADAT EKSTRAK ETANOL DAUN GEDI (Abelmoschus manihot L.) TERHADAP BAKTERI Staphylococcus epidermidis Mopangga, Elisticia; Yamlean, Paulina V.Y.; Abdullah, Surya Sumantri
PHARMACON Vol 10, No 3 (2021): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.10.2021.35605

Abstract

ABSTRACTGedi leaves (Abelmoschus manihot L.) contain metabolite compounds such as flavonoid, saponins, tannins and terpenoids for antibacterial. This study aims to formulate a solid bath soap with concentrations of 2%, 2,5% and 3% of Gedi leaves ethanol extract and to test the antibacterial activity of solid bath soap against Staphylococcus epidermidis bacteria. Experimental methods were performed for this research. For the formulation of this solid soap several experiments were conducted organoleptic test, pH, homogeneity, moisture content, free alkaline, and high foam. The effectiveness of antibacteria to inhibit Staphylococcus epidermidis growth was tested by wells method. The results showed that the ethanol extract of Gedi leaves (Abelmoschus manihot L.) can be formulated into solid bath soap that meets the requirements according to the standards set to SNI (Indonesian National Standard) with the results of physical tests of all formulas in solid or hard form and scent of rose, the distribution of colors is evenly distributed, has a pH of 8,32-9,09, a moisture content of 8,65%-8,84%, foam height from 3,7-3,8 cm, and free alkaline are in a safe condition against the skin. The results of the activity of antibacteria solid bath soap extract of the Gedi leaves were found to inhibit Staphylococcus epidermidis bacteria which has the strongest inhibition power at a concentration of 3% with an inhibition zone diameter of 14,8 mm. Keywords: Gedi Leaves (Abelmoschus manihot L.), Solid Soap, Antibacterial, Staphylococcus epidermidis.  ABSTRAKDaun Gedi (Abelmoschus manihot L.) memiliki kandungan senyawa flavonoid, saponin, tanin dan terpenoid yang bersifat sebagai antibakteri. Penelitian ini bertujuan untuk memformulasikan sediaan sabun mandi padat ekstrak etanol daun Gedi dengan konsentrasi 2%, 2,5% dan 3% serta menguji aktivitas antibakteri sediaan sabun mandi padat terhadap bakteri Staphylococcus epidermidis. Penelitian ini menggunakan metode ekperimental laboratorium. Uji mutu sediaan sabun mandi padat ekstrak etanol daun Gedi dilakukan uji organoleptik, pH, homogenitas, kadar air, alkali bebas, dan tinggi busa. Pengujian aktivitas antibakteri terhadap pertumbuhan Staphylococcus epidermidis dilakukan dengan metode sumuran. Hasil penelitian menunjukkan ekstrak etanol daun Gedi (Abelmoschus manihot L.) dapat diformulasikan menjadi sediaan sabun mandi padat yang memenuhi persyaratan sesuai dengan standar yang ditetapkan SNI (Standar Nasional Indonesia) dengan hasil semua formula berbentuk padat atau keras dan beraroma rose, penyebaran warna merata, memiliki pH 8,32-9,09, kadar air 8,65%-8,84%, tinggi busa 3,7–3,8 cm, serta alkali bebas dalam kondisi aman terhadap kulit. Hasil uji aktivitas antibakteri sediaan sabun mandi padat ekstrak etanol daun Gedi yang diperoleh dapat menghambat bakteri Staphylococcus epidermidis yang memiliki daya hambat terkuat pada konsentrasi 3% dengan diameter zona hambat 14,8 mm. Kata kunci: Daun Gedi (Abelmoschus manihot L.), Sabun Padat, Antibakteri, Staphylococcus epidermidis
FORMULASI SEDIAAN GRANUL EFFERVESCENT SARI BUAH SIRSAK (Annona muricata L) Burhan, Lisma; Yamlean, Paulina V. Y.; Supriati, Hamidah Sri
PHARMACON Vol 1, No 2 (2012)
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.1.2012.489

Abstract

Soursoup fruit nutrient includes vitamins C and B1. Vitamin which is the most dominant insoursop fruit is vitaman C, where the compound is in the form of a natural antioxidant in thebody. Antioxidants help the body to fight against various free radicals that enter the body and isexcellent for improving the immune system and slow the aging process. This study aimed toinvestigate the effect of different formulations of effervescent granules made from soursoup fruitjuice against physical properties of granules prepared by wet granulation method. Testpreparation includes organoleptic quality, moisture content, flow properties, and solubility time.Of the three formulations, variations in the quantity of citric acid, tartaric acid and sodiumbicarbonate gave effect on water levels, silence angle, and time of depression of granule made ofsoursoup fruit juice. In formulation 1, the water content was 0.19%, a flow rate of 8.2 seconds,the silence angle of 33.02 degrees, and dispersion time was 2 minutes 13 seconds.In the secondformulation, water content was 0,21%, a flow rate of 8.1 seconds, the silence angle of 31.52degrees, and dispersion time at 3 minutes 3 seconds. In the third formulation, water content was0,28%, a flow rate of 9,7 seconds, the silence angle of 31.56 degrees, and dispersion time at 4minutes 5 seconds.Key words: soursoup, effervescent granule, wet granule, natrium bicarbonate
UJI EFEK ANTIINFLAMASI EKSTRAK ETANOL DAGING BUAH LABU KUNING (Cucurbita moschata D.) TERHADAP EDEMA PADA TELAPAK KAKI TIKUS PUTIH JANTAN GALUR WISTAR (Rattus novergicus) Senewe, Merry; Yamlean, Paulina V.Y; Wiyono, Weny
PHARMACON Vol 2, No 1 (2013): pharmacon
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.2.2013.1016

Abstract

ABSTRACT Inflamation is a protective response of body from injury of cell.Edema is an indication of inflammation.Pumpkin pericarp contains of flavonoid which has potency to decrease edema.The aims of this research were to study antiinflamation effect of ethanolic extract of pumpkin pericarp (Cucurbita moschata D.) with concentration 5, 10 and 20%on edema of white rat legs strain wistar.The subjects in this research were 15 white male rat strain wistar which divided into 5 groups, namely negative control (CMC),  positive control (Piroksikam) and extract group with concentration 5, 10 and 20%.Induction of 5% of chicken albumin on rats left leg lead to edema formation, followed with induction of CMC, Piroksikam solution and extractper oral. Measurement of edema volume was done using plestimometer, and observation of edema were made every 6 hours. The result shows that there were significant administration effects of pumpkin pericarp extract with concentration 5 and 10%. As a conclusion, ethanol extract of pumpkin pericarp exhibit potency to decrease edema.
FORMULATION AND ANTIBACTERIAL ACTIVITY TEST OF COCOA BEAN EXTRACT TOOTHPASTE (Theobroma Cacao L.) AGAINST STREPTOCOCCUS MUTANS BACTERIA Apriliani Margaret Marista Lourina Rarung; Paulina Yamlean; Karlah Mansauda
PHARMACON Vol. 11 No. 4 (2022): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Cocoa beans (Theobroma cacao L.) are seeds of the cocoa tree which have a active components, is polyphenols which have the potential as antibacterial. This study aimed to formulate the toothpaste of Cocoa bean ethanol extract with concentrations of 5%, 7.5%, 10%, 12.5%, 15% and tested for physical evaluation of the preparation and to obtain the inhibitory power against Streptococcus mutans bacteria. In this research, the cocoa beans was extracted used the maceration method with 96% ethanol solvent and tested for antibacterial used the well method. The results showed an organoleptic physical evaluation requirements, homogeneous homogeneity test, pH test 7.4-7.7, spreadability test 30-33 mm, and adhesion test 2.25-3.23 seconds. Toothpaste has antibacterial activity with an average diameter of F1 (8.16 mm), F2 (8.5 mm), F3 (9.5 mm), F4 (10.41 mm) are in the medium category and F5 (11.58 mm) is in the strong category. Based on the study result can be concluded that all formulations of toothpaste preparations of Cocoa bean ethanol extract filled the antibacterial activity requirements and physical test requirements and had the strongest antibacterial activity at a concentration of 15% at 11.58 mm.
Formulation and Antibacterial Activity Test of Peel-Off Gel Mask Ethyl Acetate Fraction of Cocoa Pods (Theobroma cacao L.) against Staphylococcus aureus as Anti-Acne Immanuela Irene Mandias; Paulina Yamlean; Surya Sumantri Abdullah
PHARMACON Vol. 11 No. 4 (2022): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

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Abstract

Cocoa pods (Theobroma cacao L.) is a part of the Cocoa plant which contains active compounds such as flavonoids, alkaloids, saponins and tannins that have the potential as antibacterial. This study aimed to formulate a peel-off gel mask preparation of the ethyl acetate fraction which is physically good and to test the inhibitory power of the peel-off gel mask with concentrations of 2, 4, 8, 10, and 12% against Staphylococcus aureus. This research used laboratory experimental method. The results showed that the peel-off gel mask filled the requirements for physical evaluation, that is organoleptic, homogeneity, pH, spreadability, adhesion and drying time. The antibacterial activity test using the well diffusion method showed that the average diameter for F1 to F5 respectively was 7,5; 8,58; 12; 12,75; and 14 mm. Based on the result of the study it can be included that the overall formulation of the peel-off gel mask filled the requirements of the parameters of the physical properties of the preparation and provides the greatest antibacterial activity that is formula 5 with a concentration of 12%, which is 14 mm.
FORMULASI MOUTHWASH EKSTRAK ECENG GONDOK (Eichhornia crassipes (Mart.) Solms) SEBAGAI ANTIBAKTERI KARIES GIGI (Streptococcus mutans) Fadillah Djafar; Paulina V.Y. Yamlean; Jainer P. Siampa
PHARMACON Vol. 10 No. 4 (2021): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.10.2021.37415

Abstract

ABSTRACTWater hyacinth (Eichhornia crassipes (Mart.) Solms) contains compounds such as saponins, flavonoids, polyphenols, and alkaloids which have antibacterial properties. This study aims to make a mouthwash formulation for water hyacinth extract (E. crassipes) and to test the antibacterial activity of the preparation with an extract concentration of 5%, 10%, and 15% against the growth of Streptococcus mutans bacteria. This research uses experimental methods. The preparation formula for water hyacinth extract (E.crassipes) mouthwash is then carried out by quality testing which includes testing for organoleptic, pH, homogeneity, sedimentation test, and determination of density. The diffusion method was used to test the antibacterial activity against S. mutans growth. The results of the test of the quality of the preparations are stated in accordance with the standards. Mouthwash that has been defined, which has a clear color, distinctive smell of menthol and sweet, with a pH of 6,16; 6, 13; and 6,12. The preparations were also homogeneous without precipitates or coarse grains, the density in formulations 1, 2, and 3 was 1, 0245; 1, 0251; 1, 0253. The results of testing the antibacterial activity of water hyacinth leaf infusion extract with a concentration of 5%, 10%, and 15% showed that there was antibacterial activity, and the highest activity was at a concentration of 15% with a strong category. Keywords: Mouthwash, Water Hyacinth, Infundation, Antibacterial.  ABSTRAKEceng Gondok (Eichhornia crassipes (Mart.) Solms) memiliki kandungan senyawa saponin, flavonoid, polifenol, dan alkaloid yang bersifat sebagai antibakteri. Penelitian ini bertujuan untuk membuat formula sediaan mouthwash  ekstrak Eceng Gondok (E. crassipes) serta menguji aktivitas antibakteri sediaan dengan konsentrasi ekstrak 5%, 10%, dan 15% terhadap pertumbuhan bakteri Streptococcus mutans. Penelitian ini menggunakan metode eksperimental. Formula sediaan mouthwash ekstrak Eceng Gondok (E.crassipes) ini kemudian dilakukan pengujian mutu yang meliputi pengujian organoleptik, pH, homogenitas, uji sedimentasi, dan penetapan massa jenis. Pengujian aktivitas antibakteri terhadap pertumbuhan S.mutans dilakukan dengan metode difusi. Hasil dari uji mutu sediaan dinyatakan sesuai dengan standar Mouthwash yang ditetapkan, yakni memiliki warna yang jernih, bau khas menthol dan manis, dengan pH 6, 16; 6, 13; dan 6, 12. Sediaan juga homogen tanpa  endapan maupun butiran kasar, massa jenis pada formulasi 1, 2, dan 3 adalah 1, 0245; 1, 0251; 1, 0253.  Hasil pengujian aktivitas antibakteri ekstrak infusa daun Eceng Gondok konsentrasi 5%, 10%, dan 15% menunjukkan adanya aktivitas antibakteri, dan aktivitas tertinggi terdapat pada konsentrasi 15% dengan kategori kuat.Kata kunci: Mouthwash, Eceng Gondok, Infundasi, Antibakteri.
EFEKTIVITAS PENYEMBUHAN LUKA SAYAT GEL EKSTRAK ALGA TURBINARIA ORNATA PADA TIKUS PUTIH (Rattus norvegicus) Ravael Kurnia Kolibu; Paulina V. Y. Yamlean; Jainer Pasca Siampa
PHARMACON Vol. 11 No. 2 (2022): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.11.2022.41734

Abstract

ABSTRACT Turbinaria ornata algae is a type of algae that has known activity as antibacterial, anti-inflammatory, antioxidant activity which is in line with the mechanism of wound healing. Alga Turbinaria ornata is also one of the brown algae that contains alginate and neophytadiene compounds and has been shown to have wound healing activity. This study aimed to test the effectiveness of the ethanolic extract gel of Turbinaria ornata on wound healing in white rats. Algae extract gel of Turbinaria ornata was tested on 5 groups of rats, consisting of 3 rats in each treatment group, namely negative control, positive control, ethanolic extract gel of Algae Turbinaria ornata 5%, 10% and 15%. Observations were made until day 14. Data were tested using ANOVA (Analysis of Variant). The results of statistical tests showed that there was no significant difference in the duration of wound healing in male white rats. However, the preparation with a concentration of 15% showed faster healing data among the Turbinaria ornata algae extract gel preparations at a concentration of 5% and 10% with brand x gel and gel base. Keywords: Gel, Turbinaria ornata, White Rat, Cuts  ABSTRAK Alga Turbinaria ornate merupakan jenis alga yang meliki aktivitas yang telah diketahui sebagai antibakteri, antiinflamasi, antioksidan merupakan aktivitas yang sejalan dengan mekanisme penyembuhan suatu luka. Alga Turbinaria ornata juga merupakan salah satu alga coklat yang mengandung alginat dan senyawa neophytadiene dan telah terbukti memiliki aktivitas penyembuh luka. Penelitian ini bertujuan untuk menguji efektivitas gel ekstrak etanol Turbinaria ornata terhadap penyembuhan luka sayat pada tikus putih. Gel ekstrak Alga Turbinaria ornata diujikan pada hewan uji tikus sebanyak 5 kelompok yang terdiri dari 3 ekor tikus disetiap kelompok perlakuan, yaitu kontrol negatif, kontrol positif, gel ekstrak etanol Alga Turbinaria ornata 5%, 10% dan 15%. Pengamatan dilakukan sampai hari ke 14. Data diuji dengan menggunakan ANOVA (Analisis Of Variant). Hasil uji statistik menunjukan tidak adanya perbedaan yang signifikan terhadap lamanya penyembuhan luka pada tikus putih jantan. Meskipun, pada sedian dengan konsentrasi 15% menunjukan data penyembuhan yang lebih cepat di antara sediaan gel ekstrak alga Turbinaria ornata pada konentrasi 5% dan 10% dengan gel merek x dan basis gel. Kata Kunci: Gel, Turbinaria ornata, Tikus Putih, Luka sayat
FORMULATION AND PHYSICAL EVALUATION GEL OF AVOCADO SEED (Persea americana Mill.) AS ANTIOXIDANT USING CARBOPOL BASE CONCENTRATION Maureen Regina Satolom; Paulina Yamlean; Jainer Siampa
PHARMACON Vol. 12 No. 1 (2023): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.12.2023.42179

Abstract

Avocado seeds (persea americana mill.) contain flavonoids and have strong antioxidant activity that can help protect organs from toxic agents or oxidative stress. This study aims to formulate, evaluate and test the antioxidant effectiveness of avocado seed extract gel using the DPPH method. This research was conducted experimentally in the laboratory for making avocado seed extract gel formulations (Persea americana Mill.) with a concentration of 0.5%, a concentration of 1%, and a concentration of 1.5% carbopol base. The results showed that the avocado seed ethanol extract gel had IC50 values of 46.05 ppm, 40.01 ppm, 44.80 ppm. Based on the IC50 value obtained, it can be concluded that the avocado seed ethanol extract gel has a very strong antioxidant effectiveness. The SPPS analysis test was continued to see a significant difference but no significant difference was found, therefore the preparation with the lowest concentration will be continued with physical evaluation testing. Physical evaluation of the preparation includes organoleptic observation, pH test, dispersion test, homogeneity test, adhesion test. The results of the physical evaluation test showed that the gel preparation met the requirements.
Antibacterial Potential Test of Cream Preparation of Ethanol Extract of Matoa Stem Bark (Pometia pinnata) against Staphylococcus aureus. Violeta Gavelentri Melga Tambingon; Paulina Yamlean; Jainer Siampa
PHARMACON Vol. 12 No. 1 (2023): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.12.2023.42190

Abstract

Skin diseases can be caused by several factors, one of which is a bacterial infection. One of the bacteria that causes infection is Staphylococcus aureus. The results of previous studies showed that the ethanolic extract of the bark of matoa (Pometia pinnata) had antibacterial potential. This study aims to test the quality of cream preparations including homogeneity test, organoleptic test, pH test, adhesion test and dispersibility test, as well as to test the antibacterial activity of cream preparations ethanol extract of matoa stem bark. The matoa bark extract was obtained by maceration method using 95% ethanol as solvent. The antibacterial activity test was carried out using the well method, with a concentration variation of 0.5%, 1.5%, 2.5% and 3.5%. The test results showed that all the parameters of the test preparation, namely homogeneity test, organoleptic test, pH test, adhesion test and spreadability test met the requirements of the quality of the preparation. Antibacterial testing showed cream preparations with a concentration of 3.5% produced the largest inhibition zone of 8.66 mm which was included in the category of moderate inhibition.
Co-Authors Aaltje Manampiring Abdullah, Surya S. Abdullah, Surya Sumantri Abubakar, Poetry M.S. Abubakar, Poetry Melinda Adeanne Wullur Adithya Yudistira Amanda Putri Pratikto Anisa Puspa Juwita Antasionasti, Irma Apriliani Margaret Marista Lourina Rarung Ardiansa A.T Tucunan, Ardiansa A.T Ariem, Feiby Ba'u, Defrikson Billy Kepel Christania A. E. Pakasi Clements, Garry Daimunon, Regita Defrikson Ba'u Dewa G Katja Dewa Gede Katja Edi Suryanto Edwin de Queljoe Edwin De Queljoe Ekawati Tallei, Trina Elly Suoth Ering, Mariando N. Fabiola N Palobo Fadillah Djafar Falugah, Fathia Fatimawali . Fona Budiarso Frendsiane Pangalinan Gayatri Citraningtyas Grace Riani Pongsipulung Gratia, Berta Gusti Ayu Wulandari Hamdiyah Hamzah Hamidah Sri Supriati Hosea Jaya Edy Hutauruk, Hamido Immanuela Irene Mandias Inriani MarlinMareyke Rumayar Jainer P. Siampa Jainer Pasca Siampa Jainer Siampa Jayanto, Imam Jeane Mongi Jimmy Posangi Jonly Piere Uneputty Julianri Sari Lebang, Julianri Sari Kalalo, Tekla Karlah L. R. Mansauda Korompis, Freisy C.C Lahagina, Juan Lahagina, Juan C. G. Lakoro, Julia Elsa Lebang, Julianri S. Legoh, Dina Imorina Lempoy, Sela S Liempepas, Angelika Lisma Burhan Lolowang, Sheren N. Lomboan, Evander Roliand Majid, Nurul Majid, Nurul S Manarisip, Thesya Marhaba, Fera Anelia Maureen Regina Satolom Max R. J. Runtuwene Max R.J Runtuwene Max R.J. Runtuwene Merry Senewe Mery A R Sinaga Moilati, Veronica O. Monibala, Tiarma Mopangga, Elisticia Nau'e, Dwi A.K. Nelwan, Sabine A. Niswah Paju Novel Kojong Olivia H Naibaho, Omega Agral Pakasi, Christania A. E. Pakpahan, Kevin Yosua Pangemanan, David Albert Pingkan, Aprilia Pogaga, Eklesia Poluan, Omega A Pratasik, Meyla Pratasik, Meyla C. M. Purwoko, Agus Ratte, Titah Amelia Ravael Kurnia Kolibu Risma Meidy Hardina Sitorus Rompis, Ferrna Rumayar, Adisti Aldegonda Rumayar, Ricky C. Rundengan, Gerald Runtuwene, Kristianus Siampa, Jainer Pasca Singal, Ausich Sukandi, Gabrilia Sukandi, Gabrilia P. C. Suru, Eunike Surya S. Abdullah Surya Sumantri Abdullah Surya Sumantri Abdullah Tasya Mangkey Tatuh, Heru Andika Tiarma Tiarma TRINA EKAWATI TALLEI Umboh, Defritsevani Umboh, Defritsevani Y. Vanda S Kamu Vanda S Kamu Veren Naftalia Mamangkey Violeta Gavelentri Melga Tambingon Virginia lasut Walewangko, Marfincy Walewangko, Marfincy S. Weny I Wiyono Weny Wiyono Widdhi Bodhi Widdhi Bodhi Widya Astuty Lolo, Widya Astuty Yos Banne Yuni Arista N Kumesan