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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biopropal Industri BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Teknologi Indonesia Makara Journal of Science Makara Journal of Technology Jurnal Mahasiswa Mengabdi (JIMAWAbdi) JUPADAI : Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Ibnu Sina Jurnal Manajerial dan Bisnis Tanjungpinang Sharia Economic and Management Business Journal (SEMBJ) Journal Of Chemical Process Engineering Annales Bogorienses
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Journal : JURNAL BIOLOGI INDONESIA

 1 
Pertumbuhan Optimal Bakteri Laut Pseudomonas aeruginosa LBF-1-0132 dalam Senyawa Piren Safitriani, Safitriani; Thontowi, Ahmad; Yetti, Elvi; Suryani, Suryani; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3100

Abstract

ABSTRACTPyrene is a high molecular weight chemical compound belongs to polycyclic aromatic hydrocarbon (PAHs) group that are difficult to degrade by environment. Biodegradation techniques using indigenous marine bacteria are used to be as an effort to reduce pollutants that are carsinogenic. The objectives of this research are to screen of 18 marine bacteria isolates qualitatively by sublimation method and quantitatively by growth test and to optimize degradation activity of marine bacteria isolates by pyrene concentration and cell concentration. Identification by 16S rDNA and phylogenetic tree analysis were conducted to determine the molecular basis of bacterial identity. The result of sublimation showed that 15 isolates were positive result for pyrene degradation and classified to 3 groups. The first group consisted of 5 isolates that can produce clear zone, while the second group are 5 isolates with isolate color changes. The third group have both of activities. Growth test showed that isolate LBF-1-0132 has high potency to degrade pyrene compound. Isolate LBF-1-0132 is capable of degrading pyrene compounds optimally at concentration of 600 ppm and optimum cell concentration of 20. Based on 16S rDNA gene analysis, isolate LBF-1-0132 is Pseudomonas aeruginosa with 98% identity.Keywords :pyrene, marine bacteria, optimization, 16S rDNA identification
Isolats Bakteri Indigenous Penghasil Milk-Clotting Protease untuk Fermentasi Keju Rahmani, Nanik; Sari, Yana Nurita; Palupi, Nurheni Sri; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.729 KB) | DOI: 10.14203/jbi.v9i2.166

Abstract

The aims of this research is to isolation of bacteria that potential to produce of milk clotting protease enzymes fromfermented food that will be used as a substitute for rennet in cheese making. There are five food fermentations suchas tauco, tempeh, red oncom, sticky tape, and pickled mustard greens that are used as a source for isolation of bacteriathat could produce milk clotting protease. The results obtained four isolates proteolytic bacteria from two fermentedfood samples, three isolates bacteria from tauco (TCN 1, TCN 2, TCN 3) and one isolate from pickledmustard greens (DSN 1). Based on 16S rDNA, these isolates were identified as Bacillus sp. Bacterial isolate TCN 1has a milk clotting activity of 29.17 U/mL, whereas bacteria isolates of TCN 2, TCN 3 and DSN 1 have activities of70 U/mL achieved at the 24 hours incubation, respectively. The proteolytic activities of bacteria isolates TCN 1,TCN 2, TCN 3 and DSN 1 at the 24 hours fermentation process were 0.0117 U/mL, 0.0021 U/mL, 0.0150 U/mL,and 0.200 U/mL, respectively. The ratio of milk clotting protease activity and the proteolytic activity for bacteriaisolates TCN 1, TCN 2, TCN 3 and DSN respectively were 5402, 175000, 7292, and 3333. This showed that theenzyme from bacterial isolates TCN 2 can be used as an alternative to rennin in cheese making.Keywords: milk clotting protease, cheese, calf rennet, fermentation food
Pemurnian Parsial dan Karakterisasi Amilase dari Bakteri Laut Arthrobacter arilaitensis LBF-003 Rahmasari, Dianti; Wijanarka, Wijanarka; Pujiyanto, Sri; Rahmani, Nanik; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2323

Abstract

Starch is an abundant carbon source in nature, and ?-amylase (1, 4-?-D-glucanohydrolase; EC 3.2.1.1), which hydrolyzes ?-1, 4-glucosidic linkage in starch-related molecules. Microbe ?-amylase production is a hydrolytic enzyme and one ofinterest in its microbial production has increased dramatically due to its wide spread use in food, textile, baking anddetergent industries in recent years. Here we report ?-amylase from marine bacterium which was purified andcharacterized, as well as analyzed its hydrolysis product on starch. The enzyme of Arthrobacter arilaitensis partiallypurified by acetone precipitation with 90% and ion exchange chromatography produced specific activity 0.25 U/mg and0.38 U/mg, and it’s purity rate increased until 1.14 fold compared with former crude extract. Purifed extracelluler amilasehad an optimum activity at temperature 50°C and pH 9.0. An apparent molecular mass was between 50-75 kDa, asestimated by zimogram electrophoresis. Hydrolysis products of this enzyme on starch were maltose, maltotriose andmaltoheptaose.Keywords: alfa amylase, marine bacterium, Arthrobacter arilaitensis, purification, charaterization
Karakterisasi Biodegradasi Senyawa Poliaromatik Dibenzothiophene Oleh Bakteri Laut Novosphingobium mathurense LBF-1-0061 Tanjung, Puspasari Noerwan; Yetti, Elvi; Thontowi, Ahmad; Suprihadi, Agung; Purwantisari, Susiana; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1056.931 KB) | DOI: 10.14203/jbi.v12i2.2894

Abstract

ABSTRACTDibenzothiophene is one of polycyclic aromatic hydrocarbon (PAH) compound containing sulfur element. This compound has toxicity, mutagenic and quiet persistent in environment. From sreening test, it was known that isolate LBF-1-0061 was potential to degrade dibenzothiophene. The objectives of this study are to study dibenzotiophene degrading capability by marine bacteria isolate LBF-1-0061 using screening test; analysis of dibenzothiophene residue by GC/MS and identifiy the isolate by molecular identification. The result of this research shown that LBF-1-0061 isolate could grow up to 100 ppm of dibenzotiophene. This isolate also presented degrading capability approximately 37.5% of dibenzotiophene in 14 days incubation. Based on partial 16S rRNA gene analysis, LBF-1-0061 was identified 99% as Novosphingobium mathurense strain SM117.Keywords: sea bacteria, biodegradation, dibenzotiofen, hydrocarbon aromatic polisiclic
Keragaman Bakteri Laut Pendegradasi Alkana dan Poliaromatik Hidrokarbon di Pulau Pari Jakarta Thontowi, Ahmad; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 9, No 1 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.283 KB) | DOI: 10.14203/jbi.v9i1.154

Abstract

Minyak mentah merupakan salah satu sumber pencemaran di lingkungan laut. Degradasi oleh bakteri memegangperanan penting dalam bioremediasinya. Sejumlah 66 bakteri laut dari Pulau Pari, Kepulauan Seribu Jakarta telahdiisolasi, dianalisa berdasarkan gen 16S rDNA, dan diuji kemampuannya dalam mendegradasi minyak. Berdasarkananalisis gen 16S rDNA diperoleh lima kelompok bakteri pendegradasi minyak, yaitu α-proteobakteria (43.6%), γ-proteobakteria (48.5 %), Flavobakteria (4.5 %), Aktinobakteria (1,5 %), dan Bacillales (1,5%). Bakteribakteritersebut mampu mendegradasi komponen minyak (senyawa alkana dan poliaromatik hidrokarbon). γ-Proteobakteria dan α-proteobakteria mempunyai peran penting dalam bioremediasi minyak di kawasan lingkunganlaut di Pulau Pari. Dari hasil tersebut membuktikan bahwa bakteri pendegradasi minyak dari Pulau Pari sangatberagam.Kata kunci: minyak, laut, bakteri, bioremediasi, alkana, poliaromatik hidrokarbon
PERTUMBUHAN OPTIMAL BAKTERI LAUT PSEUDOMONAS AERUGINOSA LBF-1-0132 DALAM SENYAWA PIREN Safitriani, Safitriani; Thontowi, Ahmad; Yetti, Elvi; Suryani, Suryani; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3100

Abstract

ABSTRACTPyrene is a high molecular weight chemical compound belongs to polycyclic aromatic hydrocarbon (PAHs) group that are difficult to degrade by environment. Biodegradation techniques using indigenous marine bacteria are used to be as an effort to reduce pollutants that are carsinogenic. The objectives of this research are to screen of 18 marine bacteria isolates qualitatively by sublimation method and quantitatively by growth test and to optimize degradation activity of marine bacteria isolates by pyrene concentration and cell concentration. Identification by 16S rDNA and phylogenetic tree analysis were conducted to determine the molecular basis of bacterial identity. The result of sublimation showed that 15 isolates were positive result for pyrene degradation and classified to 3 groups. The first group consisted of 5 isolates that can produce clear zone, while the second group are 5 isolates with isolate color changes. The third group have both of activities. Growth test showed that isolate LBF-1-0132 has high potency to degrade pyrene compound. Isolate LBF-1-0132 is capable of degrading pyrene compounds optimally at concentration of 600 ppm and optimum cell concentration of 20. Based on 16S rDNA gene analysis, isolate LBF-1-0132 is Pseudomonas aeruginosa with 98% identity.Keywords :pyrene, marine bacteria, optimization, 16S rDNA identification
ISOLATS BAKTERI INDIGENOUS PENGHASIL MILK-CLOTTING PROTEASE UNTUK FERMENTASI KEJU Rahmani, Nanik; Sari, Yana Nurita; Palupi, Nurheni Sri; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i2.166

Abstract

The aims of this research is to isolation of bacteria that potential to produce of milk clotting protease enzymes fromfermented food that will be used as a substitute for rennet in cheese making. There are five food fermentations suchas tauco, tempeh, red oncom, sticky tape, and pickled mustard greens that are used as a source for isolation of bacteriathat could produce milk clotting protease. The results obtained four isolates proteolytic bacteria from two fermentedfood samples, three isolates bacteria from tauco (TCN 1, TCN 2, TCN 3) and one isolate from pickledmustard greens (DSN 1). Based on 16S rDNA, these isolates were identified as Bacillus sp. Bacterial isolate TCN 1has a milk clotting activity of 29.17 U/mL, whereas bacteria isolates of TCN 2, TCN 3 and DSN 1 have activities of70 U/mL achieved at the 24 hours incubation, respectively. The proteolytic activities of bacteria isolates TCN 1,TCN 2, TCN 3 and DSN 1 at the 24 hours fermentation process were 0.0117 U/mL, 0.0021 U/mL, 0.0150 U/mL,and 0.200 U/mL, respectively. The ratio of milk clotting protease activity and the proteolytic activity for bacteriaisolates TCN 1, TCN 2, TCN 3 and DSN respectively were 5402, 175000, 7292, and 3333. This showed that theenzyme from bacterial isolates TCN 2 can be used as an alternative to rennin in cheese making.Keywords: milk clotting protease, cheese, calf rennet, fermentation food
PEMURNIAN PARSIAL DAN KARAKTERISASI AMILASE DARI BAKTERI LAUT ARTHROBACTER ARILAITENSIS LBF-003 Rahmasari, Dianti; Wijanarka, Wijanarka; Pujiyanto, Sri; Rahmani, Nanik; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2323

Abstract

Starch is an abundant carbon source in nature, and ?-amylase (1, 4-?-D-glucanohydrolase; EC 3.2.1.1), which hydrolyzes ?-1, 4-glucosidic linkage in starch-related molecules. Microbe ?-amylase production is a hydrolytic enzyme and one ofinterest in its microbial production has increased dramatically due to its wide spread use in food, textile, baking anddetergent industries in recent years. Here we report ?-amylase from marine bacterium which was purified andcharacterized, as well as analyzed its hydrolysis product on starch. The enzyme of Arthrobacter arilaitensis partiallypurified by acetone precipitation with 90% and ion exchange chromatography produced specific activity 0.25 U/mg and0.38 U/mg, and it?s purity rate increased until 1.14 fold compared with former crude extract. Purifed extracelluler amilasehad an optimum activity at temperature 50°C and pH 9.0. An apparent molecular mass was between 50-75 kDa, asestimated by zimogram electrophoresis. Hydrolysis products of this enzyme on starch were maltose, maltotriose andmaltoheptaose.Keywords: alfa amylase, marine bacterium, Arthrobacter arilaitensis, purification, charaterization
KARAKTERISASI BIODEGRADASI SENYAWA POLIAROMATIK DIBENZOTHIOPHENE OLEH BAKTERI LAUT NOVOSPHINGOBIUM MATHURENSE LBF-1-0061 Tanjung, Puspasari Noerwan; Yetti, Elvi; Thontowi, Ahmad; Suprihadi, Agung; Purwantisari, Susiana; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2894

Abstract

ABSTRACTDibenzothiophene is one of polycyclic aromatic hydrocarbon (PAH) compound containing sulfur element. This compound has toxicity, mutagenic and quiet persistent in environment. From sreening test, it was known that isolate LBF-1-0061 was potential to degrade dibenzothiophene. The objectives of this study are to study dibenzotiophene degrading capability by marine bacteria isolate LBF-1-0061 using screening test; analysis of dibenzothiophene residue by GC/MS and identifiy the isolate by molecular identification. The result of this research shown that LBF-1-0061 isolate could grow up to 100 ppm of dibenzotiophene. This isolate also presented degrading capability approximately 37.5% of dibenzotiophene in 14 days incubation. Based on partial 16S rRNA gene analysis, LBF-1-0061 was identified 99% as Novosphingobium mathurense strain SM117.Keywords: sea bacteria, biodegradation, dibenzotiofen, hydrocarbon aromatic polisiclic
KERAGAMAN BAKTERI LAUT PENDEGRADASI ALKANA DAN POLIAROMATIK HIDROKARBON DI PULAU PARI JAKARTA Thontowi, Ahmad; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 9, No 1 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i1.154

Abstract

Minyak mentah merupakan salah satu sumber pencemaran di lingkungan laut. Degradasi oleh bakteri memegangperanan penting dalam bioremediasinya. Sejumlah 66 bakteri laut dari Pulau Pari, Kepulauan Seribu Jakarta telahdiisolasi, dianalisa berdasarkan gen 16S rDNA, dan diuji kemampuannya dalam mendegradasi minyak. Berdasarkananalisis gen 16S rDNA diperoleh lima kelompok bakteri pendegradasi minyak, yaitu α-proteobakteria (43.6%), γ-proteobakteria (48.5 %), Flavobakteria (4.5 %), Aktinobakteria (1,5 %), dan Bacillales (1,5%). Bakteribakteritersebut mampu mendegradasi komponen minyak (senyawa alkana dan poliaromatik hidrokarbon). γ-Proteobakteria dan α-proteobakteria mempunyai peran penting dalam bioremediasi minyak di kawasan lingkunganlaut di Pulau Pari. Dari hasil tersebut membuktikan bahwa bakteri pendegradasi minyak dari Pulau Pari sangatberagam.Kata kunci: minyak, laut, bakteri, bioremediasi, alkana, poliaromatik hidrokarbon
Co-Authors A.A. Ketut Agung Cahyawan W ADE ANDRIANI Agung Pranata, Gandi Ahmad Thontowi Anam, M. Khairul Anja Meryandini Awan Purnawan Bambang Prasetya Budiawan Budiawan Dewi, Fitria DWI SUSILANINGSIH Elvi Yetti, Elvi Endang Sukara Gulo, Happy Christman Hapzi Ali Harayama, Shigeaki Harayama, Shigeaki Harwati, Theresia Umi Helprida Simamora Heryenzus, Heryenzus Hidayat, Haris Suganda Ilmiah, Zidny Jonmaianto Sihombing La Ifa Maggy T Suhartono Maggy Thenawidjaja Suhartono Martin, Andri Fadillah Muhammad Iqbal NANIK RAHMANI Nuryana, Isa Nuryati Nuryati Nuzul Farini, Nuzul Okazaki, Fumiyoshi Okazaki, Fumiyoshi Oktavianti Oktavianti Palupi, Nurheni Sri Purwoko, Agus PUSPITA LISDIYANTI Putra, Filemon Jalu Nusantara Putri, Feby Heryani Rahmasari, Dianti Rahmasari, Dianti Riznando, Reza Rohmattusolihat Rohmattusolihat, Rohmattusolihat ROHMATUSSOLIHAT ROHMATUSSOLIHAT Ruth Melliawati Safitriani, Safitriani Safitriani, Safitriani Sari, Yana Nurita Sari, Yana Nurita Septa Diana Nabella SHANTI RATNAKOMALA Sihombing, Jonmaianto Simanjuntak, Jackhon Pahala Sita Heris Anita Situmorang, Robert Sri Hartati Sri Pujiyanto Suhanda La Tima, Suhanda Sulastri Sulastri Suryani Suryani Susiana Purwantisari Tanjung, Puspasari Noerwan Tanjung, Puspasari Noerwan Theresia Harwati, Theresia Tun Tedja Irawadi Tutik Murniasih Urip Perwitasari, Urip Wahyudi, Muhammad Eko WIBOWO MANGUNWARDOYO Wijanarka Wijanarka Wijaya, Hans Yantyati Widyastuti