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All Journal Vegetalika Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Agrotrop : Journal on Agriculture Science Jurnal Pengendalian Hayati BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIKA Cropsaver : Journal of Plant Protection Plumula : Berkala Ilmiah Agroteknologi Proceeding International Conference on Global Resource Conservation Jurnal Agroekoteknologi BIOEDUSCIENCE Jurnal SOLMA Jurnal Pertanian Agros Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Agrotek Tropika Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) JURNAL AGROHITA Jurnal Ilmiah RESPATI Jurnal Pembelajaran dan Biologi Nukleus Agrienvi : Jurnal Ilmu Pertanian Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis Nusantara Science and Technology Proceedings Bioscientist : Jurnal Ilmiah Biologi Berkala Penelitian Hayati AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Eduvest - Journal of Universal Studies Journal of Applied Plant Technology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Arika Purnawati
Universitas Pembangunan Nasional Veteran Jawa Timur
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Effectiveness of Alternative Media for the Mass Rearing of Trichoderma sp. in Controlling Anthracnose Colletotrichum sp. Disease on Red Chili Plant Maulidya, Aisyah; Purnawati, Arika; Mujoko, Tri
BIOEDUSCIENCE Vol 10 No 2 (2026): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/22059

Abstract

Background: Anthracnose caused by Colletotrichum sp. is one of the major diseases limiting red chili production. Trichoderma sp. is a promising biological control agent, but its large-scale application requires suitable propagation media. Methods: This study aimed to evaluate alternative media for the mass propagation of Trichoderma sp. and the suppression of anthracnose through in vitro and in vivo experiments using a Completely Randomized Design (CRD). The in vitro assay involved an antagonism test using five treatments with four replications, measuring the inhibitory effect via the dual culture method. The in vivo assay assessed the incubation period and disease intensity using five treatments with four replications each, yielding 20 experimental units. Each experimental unit consisted of five plant samples, making a total of 100 plants. The collected data were analyzed using ANOVA and the Honestly Significant Difference (HSD) test at a 5% significance level. Results: Alternative media affected the growth and effectiveness of Trichoderma sp. In the in vitro test, inhibition against Colletotrichum sp. ranged from 30.67% to 52.58% at 5 days after inoculation. In the in vivo test, disease intensity at 84 days after planting ranged from 6.25% to 22.50% in treated plants, compared with 28.33% in the control. Corn-rice and rice-bran media showed the most favorable overall performance in supporting propagation and disease suppression. Conclusions: Alternative media can support the propagation of Trichoderma sp. and maintain its antagonistic potential against anthracnose in red chili. Corn-rice and rice-bran media showed the best overall potential among the tested media.
Co-Authors . NUGROHORINI . WAGIYANA Ahmad Adibul Akrom Ahmad Davin Najwan Adhyasta Akrom, Ahmad Adibul Al Rahmad, Nirmala Putri Alfa, Maria Rodhya Althaf, Marcellino Alvia Cindy Apriliani Amalia, Anisa Rizki Aris Cahyono Arkan Haykal Augustien, Nora Bambang HADISUTRISNO Diasi, Ananta Rizki Didik Sulistyanto Dwirizki Permata, Aprilla Hendiana Elly Syafriani Fari A, Haidar Fatimah Nabila Zahra Firmansyah, Moch Ryan Hadi Suhardjono Haidar Fari Aditya Hasni Wardahni Heriyati, Sukma Herry Nirwanto Herry Nirwanto Iis Purnamawati Indah Pemuda Indah, Pawana Nur Indarwati Indarwati Irsyad Irsyad irsyad, irsyad Irsyad, Irsyad Ketut Srie Marhaeni Julyasih Khairunnisa, Nabila Zahra Kurniawati Kurniawati Kusumawati, Diah Budi Laras Setyowati Laras Setyowati Maharani, Salsabilla Diva Marcellino Althaf Maria Rodhya Alfa Maulidya, Aisyah Maulina Aizah, Shinta Muhammad Raihan Naufal Mujoko , Tri Mukaromah, Fathul Nensi Agustina Nensi Agustina Nensi Agustina Nijma Nurfadila Noni Rahmadhini Nora Augustien Novi Nafisah Najmi Nur Aini Mahfud Nur Aini Mahfud Nurul Afida Nurul Laily Pawana Nur Indah Penta SURYAMINARSIH Penta Suryaminarsih Penta Suryaminarsih Prasetyawati, Endang Triwahyu Prasetyowati, Endang Triwahyu Presti Mardiyani Pribadi, Didik Utomo RAMADHANI MAHENDRA KUSUMA Rizky Ika Noviyanti Rizky Ika Noviyanti Saefur Rohman Safira Rizka Lestari Satrio Hanif Haryawan Sayekti, Ninik Ari Setyowati, Laras Sri Wiyatiningsih Sri Wiyatiningsih Sri Wiyatiningsih Suhardjono , Hadi Syamsul Arifin Tri Mudjoko Tri MUJOKO Triwahyu P, Endang Triwidodo ARWIYANTO USWATUN HASANAH Wafiah Huwaina Zata Dini Widayati, Wilujeng Widyananda, Kanaya Syafina Wilujeng Widayati Windriyanti, Wiwin Wiwik Harjani Yanti, Vika Yenny Wuryandari Yenny WURYANDARI Yerni Andryani Yuningsih Yuningsih Zakaria Wildan Fachrezzy Zakqy, Nauval