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All Journal Vegetalika Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Agrotrop : Journal on Agriculture Science Jurnal Pengendalian Hayati BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIKA Cropsaver : Journal of Plant Protection Plumula : Berkala Ilmiah Agroteknologi Proceeding International Conference on Global Resource Conservation Jurnal Agroekoteknologi BIOEDUSCIENCE Jurnal SOLMA Jurnal Pertanian Agros Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Agrotek Tropika Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) JURNAL AGROHITA Jurnal Ilmiah RESPATI Jurnal Pembelajaran dan Biologi Nukleus Agrienvi : Jurnal Ilmu Pertanian Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis Nusantara Science and Technology Proceedings Bioscientist : Jurnal Ilmiah Biologi Berkala Penelitian Hayati AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Eduvest - Journal of Universal Studies Journal of Applied Plant Technology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Arika Purnawati
Universitas Pembangunan Nasional Veteran Jawa Timur
Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Social Sciences Veterinary Other

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Secondary Metabolites of Trichoderma sp. as Antifungal Against Rice Seed-borne Pathogen Fungi Amalia, Anisa Rizki; Purnawati, Arika; Prasetyawati, Endang Triwahyu; Yanti, Vika
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4822

Abstract

The use of quality seeds is necessary to increase rice productivity. The existence of pathogenic fungi infections causes the quality of rice seeds to decrease and impacts the ability to grow plants. Trichoderma sp. is known to produce secondary metabolites that can inhibit the growth of pathogens. This study aimed to examine the ability of the secondary metabolites of Trichoderma sp. at various concentration levels and immersion time to suppress pathogenic fungi infections carrying rice seeds. The research was carried out by giving seed immersion treatment. Then identify the pathogenic fungi the seeds carry for further testing with the blotter test method. The parameters tested were infection rate, infection suppression, and seed viability. The identification results showed types of pathogenic fungi carried by rice seeds included Fusarium sp., Alternaria sp., Aspergillus sp. Penicillium sp., and Rhizopus sp. Research also shows that the secondary metabolites of Trichoderma sp. are effective in improving the quality of rice seeds. The test results showed that the secondary metabolites of Trichoderma sp. can suppress the level of pathogenic fungi infection. There was no interaction between the treatment of metabolite concentrations and immersion time on all test parameters. Seed immersion treatment with secondary metabolites in the 5-20% concentration range has effectively reduced fungi infection rates and increased seed viability. Meanwhile, various levels of soaking time did not affect reducing the level of fungi infection but instead affected the viability of the rice seeds.
Potential of Endophytic Fusarium sp. Against Bacterial Wilt Disease in Tomato Plants Nur Aini Mahfud; Ahmad Adibul Akrom; Arika Purnawati
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.4008

Abstract

Ralstonia solanacearum is a pathogen that causes bacterial wilt in tomato plants (Lycopersicum esculentum), can decrease production until 90%. The controls generally use chemical pesticides which are harmful to the environment and humans, so it is necessary alternative controls using endophytic Fusarium sp. The aim of the research was to determine the effect of endophytic Fusarium sp. to bacterial wilt. Endophytic Fusarium sp. was explored on tomato plant in Tlekung, Junrejo-Batu and taken systematically randomly then brought to the laboratory. Isolation of endophytic fungi was done by washing the plants with running water, cutting 1cm then was sterilized. The sample was planted on Potato Dextrose Agar (PDA) and incubated at 37°C for 48 hours, if its growth was purified on new media. Isolation of R. solanacearum was done by washing the stems, cutting 0.5 cm then sterilizing. The piece is put into a test tube, added 10 ml of sterile water until the bacterial mass comes out and dissolves in sterile water. The mass of bacteria in sterile water is shaken using vortex until suspension forms. The suspension was grown on TZC agar and incubated at 280C for 24 hours, if it grow was purified on new media. Application of endophytic Fusarium sp. carried out using immersing technique of the roots of tomato plant. Inoculation of R. solanacearum was done into sterile soil before the plant were planted. The study used completely randomized design (CRD) with control and isolate of endophytic Fusarium sp. as treatments with 3 repeat and conducted in the greenhouse of the Faculty of Agriculture UPN "Veteran" East Java. Observations are incubation period and disease intensity, data were analyzed using BNT 5%. The results show that endophytic Fusarium sp. delayed the period incubation and suppressed the intensity of bacterial wilt disease in tomato plants.
The Effect of Water Activity on the Growth of Aspergillus flavus Laras Setyowati; Nijma Nurfadila; Arika Purnawati
Nusantara Science and Technology Proceedings The 4th International Conference on Agriculture and Environmental Sciences (ICAES) 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.3904

Abstract

Aspergillus flavus is recognized as one of the fungi responsible for inducing diseases and causing damage to both food and feed commodities. This fungus is capable of producing mycotoxins that possess toxic attributes to both humans and animals. The contamination by A. flavus spans a broad spectrum, commencing from field cultivation and extending to storage facilities. One alternative approach to manage this fungus involves the modification of its growth environment. Microorganisms inherently possess a minimum water activity (aw) value crucial for their metabolic processes. The objective of this study is to modify the aw value to inhibit the growth of A. flavus. This research was conducted in vitro using PDA media supplemented with glycerol and distilled water to establish aw conditions of 0.90, 0.92, 0.95, and 0.97. On the seventh day after incubation, the results demonstrated that for 0.90 aw exhibited a significant inhibition on A. flavus growth, with an average colony diameter is 1.34 mm, followed by 0.92 is 1.54 then 0.95 is 1.83 mm, and 0.97 is 1.84 mm. Conversely, treatments with 0.90 displayed the lowest inhibitions (1.34 mm) and 0.97 displayed the highest (1.84 mm). All modified water activity treatments exerted an impact on the growth of A. flavus. As the aw value decreases, the growth of A. flavus becomes increasingly constrained.
Effect of Formula Endophytic Bacteria in Organic Fertilizer to Wilt Disease Fusarium in Chili Plants Laras Setyowati; Arika Purnawati; Tri Mujoko
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3103

Abstract

Chili (Capsicum annum) is one of the horticultural commodities that has an important role in human life and Indonesian chili plants have important economic value and occupy second place after beans. Meanwhile, in East Java, chili commodity production in 2013-2017 reached 440 tons and one of the highest contributors was Ringinsari, Kandat, Kediri. However, the increase in chili commodities in the village has so far been strongly influenced by the presence of the fungi pathogen is Fusarium oxysporum f.sp.capsici that cause wilt disease, can reduce production on average up to 75 % and cause the plant to die. The control of that pathogen generally uses chemical control that hurts humans and the environment so until now it have not been satisfactory. Depend of that it needs alternative control using endophytic bacteria that are formulated in organic fertilizer as biological control. Endophytic bacteria are group of bacteria that live in plant tissues without causing negative effects, including not causing plants to get sick and having a mutual relationship with their host plants. Endophytic bacteria can be found in various plants including agricultural crops and can be isolated from all parts of the plant, namely roots, stems, leaves, and seeds. Endophytic bacteria produce secondary metabolites and compounds that can be used to induce plant health and growth. Endophytic bacteria produce fenol, flavonoid that increase plant resistance to diseases and pests, improve plant health, phytohormones increase plant growth. The method stages of research are propagation of endophytic bacteria, manufacture of suspension of endophytic bacteria, mixing of suspension of endophytic bacteria into organic fertilizer, data analysis using analysis of variance and 5% BNT test. The results are Incubation period is slower, disease intensity decreases, and plant height increases.
Endophytic Bacteria from Wet Soil of South Kalimantan as Biological Control Agent for Root Nematodes (NPA) in Celery (Apium graveolens) Marcellino Althaf; Wilujeng Widayati; Arika Purnawati
Nusantara Science and Technology Proceedings Seminar Nasional Magister Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3203

Abstract

Endophytic bacteria are microorganisms that are associated with plants, don’t cause disease, and mutualism associated with plants. One of the uses of endophytic bacteria in agriculture is as biological agent in overcoming the attack of the root-knot nematode Meloidogyne sp. It is a polyphagous parasite that can attack plants by forming galls on the roots of celery plants (A. graveolens), can lack nutrients, and in chronic conditions causes death of host plants. It’s control is important to reduce crop yield loss. Control of the nematode Meloidogyne sp., at this time use many chemical pesticides because they quickly kill nematodes, but the continuous use of chemical nematicides will potentially pollute the environment. So, it is necessary to study alternative control using endophytic bacteria. The purpose of this study was to determine the potential of endophytic bacteria from wetlands of South Kalimantan as biological agents of it in celery plants. This research was conducted in Sumber Glagah, Pacet, Mojokerto, using Completely Randomized Design (CRD). The treatment was carried out by immersing the roots of celery plants in a suspension of endophytic bacteria (24 hours) and concentration (108 cfu/ml), then planted in polybags measuring 30 x 30. The polybags were filled with soil that had been divested with 50 juvenile Meloidogyne sp. Observation parameters were the number of galls per g of roots and juvenile population per g of root and per 10 g of soil. The results showed that the treatment of endophytic bacteria reduce: (1) number gall per g celery roots was 19.67–31.00 and control (46.67); (2) population juvenile population per g root was 9.33-25.67 and control 50.67; (3) juvenile population per 10 g of soil was 11.33-17.00 and control 29.33.
Natural Ingredients of Beans as Growth Medium and Propagation of Endophytic Bacteria from Lowland Eggplant Arika Purnawati; Safira Rizka Lestari; Iis Purnamawati
Nusantara Science and Technology Proceedings 7st International Seminar of Research Month 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3308

Abstract

Endophytic bacteria are defined as a group of microbes that live in plant tissues that do not cause disease and are mutually related to the host plant, can be isolated from all parts of the plant were roots, stems, leaves, and seeds. Endophytic bacteria have the potential to produce natural products for agriculture, increase plant growth, and support the sustainable development of agriculture. The growth of endophytic bacteria in vitro generally uses a medium that contains nutrients including macronutrients, namely elements (C, H, O, and N), micronutrients, namely non-metallic elements (S and P), metallic elements (Ca, Zn, Na, K, Cu). , Mn, Mg, and Fe), vitamins, water, and energy. To meet these growth requirements, currently the growth and propagation of endophytic bacteria generally still use synthetic media which are expensive and in the field, mainly by farmers, are not easy to obtain. The study aimed to obtain an alternative medium made from beans for the growth and propagation of endophytic bacteria from lowland eggplants. The types of beans were soybeans, green beans, red beans, cowpeas, peanuts, and Nutrient Agar (NA) as control. Inoculation into the medium was done using the spread plate technique. This technique is preceded by a dilution and at dilution of 10-7 was taken 0.1 ml then to be spread on the growing medium. Observations were growing and colony counting was done 24 hours after inoculation. The results were: (1) endophytic bacteria from lowland eggplant can grow well on all mediums with the best growth on NA medium, (2) all media can be used as the propagation of endophytic bacteria from lowland eggplant plants, (3) the assumed of the growth curve is logarithmic with 4 phases were lag phase, exponential phase, stationer phase, death phase.
Formulation of Endophytic Bacteria in Organic Fertilizer as Health Promoter for Corn Plants in Mentaos, Gudo, Jombang Arika Purnawati; Tri Mujoko; Endang Triwahyu Prasetyawati
Nusantara Science and Technology Proceedings 7st International Seminar of Research Month 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3309

Abstract

Corn (Zea mays) has an important role in the food supply in Indonesia, as the second commodity after rice and the second source of carbohydrates after rice which is a derivative of rice and is still considered feasible to meet national needs. The increase in national corn production is strongly supported by several corn production center provinces such as East Java and one of the districts that have the potential to support corn production in East Java is Jombang, but the reality the production experiences several obstacles, one of which is the presence of plant pest organisms (OPT). namely the fungus Peronosclerospora maydis caused by downy mildew. In the control of disease so far in the field, many use fungicides that are harmful to humans and the environment. The solution to reduce the use of fungicides is to utilize endophytic bacteria from eggplant and it has been formulated with organic fertilizer so that it is easier to apply to corn plants, this solution is expected to control disease rates, reduce losses, and improve corn plant health. The method used in community service in Mentaos, Gudo, and Jombang were situation analysis, problem identification, socialization that was attended by 25 farmers, and application of endophytic bacteria from eggplant plants in organic fertilizer formulas. The results obtained are that the people of Mentaos, Gudo, and Jombang were able to apply the endophytic bacterial formula in organic fertilizer with the sprinkle technique. The results show that the community can apply the endophytic bacterial formula, corn plants become healthy, and disease intensity is low.
Potensi Bioenkapsulasi Bakteri Endofit Bacillus sp. sebagai Biokontrol Busuk Batang Fusarium pada Tanaman Jagung Akrom, Ahmad Adibul; Purnawati, Arika; Prasetyowati, Endang Triwahyu
Jurnal Agroekoteknologi Vol 16, No 2 (2024)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v16i2.27488

Abstract

Busuk batang fusarium merupakan salah satu penyakit penting pada tanaman jagung. Busuk batang dapat mengakibatkan penurunan produktivitas. Saat ini, banyak fungisida kimia yang digunakan untuk mengendalikan berbagai penyakit pada tanaman yang disebabkan oleh Fusarium sp. Penggunaan fungisida kimia secara terus-menerus dapat menimbulkan kerusakan lingkungan dan menimbulkan resistensi patogen. Penggunaan agens pengendali hayati secara langsung selama ini sering dilakukan, namun hasilnya kurang efektif. Oleh karena itu, penelitian ini bertujuan untuk meningkatkan efektivitas agens pengendali hayati dalam menghambat penyakit tersebut melalui bioenkapsulasi. Diharapkan hasil penelitian tentang potensi bioenkapsulasi bakteri endofit Bacillus sp. ini bermanfaat sebagai rekomendasi pengendalian penyakit busuk batang fusarium. Aplikasi bioenkapsulasi beads bakteri endofit Bacillus sp. sebagai pengendali hayati dapat menjadi alternatif dalam pengendalian penyakit busuk batang fusarium pada tanaman jagung dibandingkan dengan penggunaan fungisida kimia berbahan aktif prophineb. Hasil penelitian menunjukkan bahwa perlakuan K3 (konsentrasi natrium alginat 3%) memperoleh hasil tertinggi pada pengujian viabilitas dan efisiensi enkapsulasi, yang berpengaruh terhadap nilai intensitas penyakit, serta nilai tertinggi pada pengujian rumah kaca. Hasil yang diperoleh juga tidak berbeda nyata dengan perlakuan menggunakan fungisida kimia. 
Characterization of Bacterial Blight Pathogen on Rice Plants in Sidoarjo Sayekti, Ninik Ari; Purnawati, Arika; Lestari, Safira Rizka
Journal of Applied Plant Technology Vol 3 No 2 (2024): Journal of Applied Plant Technology (JAPT)
Publisher : Agrotechnology Study Program, Faculty of Agriculture, Wijaya Kusuma Surabaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/xwdype52

Abstract

Rice (Oryza sativa L.) is a staple crop for all Indonesian people. Rice production in Indonesia in 2019 experienced a decline in production. Plant disease attacks are a factor that influences the decline in rice production. Bacterial leaf blight is an important disease in rice plants that can cause yield losses of up to 50%. Characterization of disease-causing bacteria needs to be carried out to develop appropriate, effective and efficient control in suppressing the development of bacterial leaf blight. The aim of this research was to characterize the morphological and physiological characteristics of the bacteria that cause bacterial leaf blight. The method used in this research included the isolation of bacteria that cause bacterial leaf blight disease carried out on rice plantations in Pulungan Village, Sedati, Sidoarjo. Identification is carried out through macroscopic and microscopic observations, analysis of physiological properties through the gram staining test, fermentative oxidative test, yellow colony test on YDC media, urease test, hypersensitivity test, and pathogenicity test. The results of the research show that the bacteria that cause bacterial leaf blight have the morphological characteristics of being yellow, round and shiny. The physiological characteristics of the bacterial isolate are gram-negative bacteria, are oxidative, yellow in YDC media, and react negatively in the urease test.
Potential Trichoderma sp. from Peat Soil in Controlling Seed-Borne Pathogens and Growth Stimulator in Soybean (Glycine max L.) Diasi, Ananta Rizki; Purnawati, Arika; Wiyatiningsih, Sri
BIOEDUSCIENCE Vol 8 No 3 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/14563

Abstract

Background: Seed-borne pathogens threaten plants because they can survive in seed tissues for long periods. Efforts that can be made include treating the seeds before planting them in the field with biological agents, one of which is Trichoderma sp. It is often found in soil with high organic matter, namely peat soil. This research aims to determine the potential of Trichoderma sp. isolates. from peat soil on pathogen infection of soybean seeds and germination of soybean seeds. Method: This research used a factorial method. The first factor was sick and healthy seeds, and the second was Trichoderma sp. isolates, consisting of TP1 and TP2. The control treatment uses the active ingredient fungicide mancozeb 80%. Seed treatment was carried out by soaking the seeds in a suspension of Trichoderma sp. with a spore density of 106 for 24 hours. The seeds were then tested using the growing-on-test method in sterile soil. Results: Identifying pathogens in soybean seeds found two genera, Aspergillus sp., and Fusarium sp., in the immersion treatment on Trichoderma sp isolates. TP2 gave the best results, where the lowest infection power was 46.6% for healthy seeds and 73.3% for symptomatic seeds. In addition, treatment with isolates of Trichoderma sp. TP2 can increase the germination capacity of soybean seeds by 60.0%. However, seed treatment with Trichoderma sp. does not affect soybean plant height and root length growth. Conclusion: The two isolates of Trichoderma sp. from peat soil positively protect seeds against soybean seed pathogens, seed germination, and soybean growth.
Co-Authors . NUGROHORINI . WAGIYANA Ahmad Adibul Akrom Ahmad Davin Najwan Adhyasta Akrom, Ahmad Adibul Al Rahmad, Nirmala Putri Alfa, Maria Rodhya Althaf, Marcellino Alvia Cindy Apriliani Amalia, Anisa Rizki Aris Cahyono Arkan Haykal Augustien, Nora Bambang HADISUTRISNO Diasi, Ananta Rizki Didik Sulistyanto Dwirizki Permata, Aprilla Hendiana Elly Syafriani Fari A, Haidar Fatimah Nabila Zahra Firmansyah, Moch Ryan Hadi Suhardjono Haidar Fari Aditya Hasni Wardahni Heriyati, Sukma Herry Nirwanto Herry Nirwanto Iis Purnamawati Indah Pemuda Indah, Pawana Nur Indarwati Indarwati Irsyad Irsyad Irsyad, Irsyad irsyad, irsyad Ketut Srie Marhaeni Julyasih Khairunnisa, Nabila Zahra Kurniawati Kurniawati Kusumawati, Diah Budi Laras Setyowati Laras Setyowati Maharani, Salsabilla Diva Marcellino Althaf Maria Rodhya Alfa Maulidya, Aisyah Maulina Aizah, Shinta Muhammad Raihan Naufal Mujoko , Tri Mukaromah, Fathul Nensi Agustina Nensi Agustina Nensi Agustina Nijma Nurfadila Noni Rahmadhini Nora Augustien Novi Nafisah Najmi Nur Aini Mahfud Nur Aini Mahfud Nurul Afida Nurul Laily Pawana Nur Indah Penta Suryaminarsih Penta SURYAMINARSIH Penta Suryaminarsih Prasetyawati, Endang Triwahyu Prasetyowati, Endang Triwahyu Presti Mardiyani Pribadi, Didik Utomo RAMADHANI MAHENDRA KUSUMA Rizky Ika Noviyanti Rizky Ika Noviyanti Saefur Rohman Safira Rizka Lestari Satrio Hanif Haryawan Sayekti, Ninik Ari Setyowati, Laras Sri Wiyatiningsih Sri Wiyatiningsih Sri Wiyatiningsih Suhardjono , Hadi Syamsul Arifin Tri Mudjoko Tri MUJOKO Triwahyu P, Endang Triwidodo ARWIYANTO USWATUN HASANAH Wafiah Huwaina Zata Dini Widayati, Wilujeng Widyananda, Kanaya Syafina Wilujeng Widayati Windriyanti, Wiwin Wiwik Harjani Yanti, Vika Yenny WURYANDARI Yenny Wuryandari Yerni Andryani Yuningsih Yuningsih Zakaria Wildan Fachrezzy Zakqy, Nauval