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All Journal Vegetalika Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Agrotrop : Journal on Agriculture Science Jurnal Pengendalian Hayati BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIKA Cropsaver : Journal of Plant Protection Plumula : Berkala Ilmiah Agroteknologi Proceeding International Conference on Global Resource Conservation Jurnal Agroekoteknologi BIOEDUSCIENCE Jurnal SOLMA Jurnal Pertanian Agros Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Agrotek Tropika Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) JURNAL AGROHITA Jurnal Ilmiah RESPATI Jurnal Pembelajaran dan Biologi Nukleus Agrienvi : Jurnal Ilmu Pertanian Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis Nusantara Science and Technology Proceedings Bioscientist : Jurnal Ilmiah Biologi Berkala Penelitian Hayati AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Eduvest - Journal of Universal Studies Journal of Applied Plant Technology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Arika Purnawati
Universitas Pembangunan Nasional Veteran Jawa Timur
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Potensi Trichoderma sp. Asal Tanah Gambut Sebagai Agensia Pengendali Biologi Jamur Patogen Terbawa Benih Padi Maulina Aizah, Shinta; Purnawati, Arika; Prasetyawati, Endang Triwahyu
BIOEDUSCIENCE Vol 8 No 3 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/15354

Abstract

Background: Rice (Oryza sativa) is the world's second most important food crop after wheat. In Indonesia, more than 90% of the population consumes rice, and there is a very high level of dependence, so rice has essential value for the Indonesian population. However, this has not been accompanied by the availability of healthy seeds because many pathogenic fungi are carried by rice seeds, such as Rhizopus sp., Mucor sp., Aspergillus sp., Fusarium sp., Curvularia sp. Seed treatment using synthetic fungicides is an effort to control pathogenic fungi on seeds, but it hurts seeds, humans, and the environment. A safe control alternative is using biological agents, namely the fungus Trichoderma sp. isolated from peatlands and has an antibiosis mechanism. The research aimed to test the potential of the fungus Trichoderma sp. from peat soil as a biological control agent for pathogenic fungi carried by rice seeds (Oryza sativa). Methods: The test uses the incubation method on PDA media and the growing test method on sterile soil media. The research used a completely randomized design with a factor of 2 isolates of Trichoderma sp. and eight replications and data analysis using BNJ5%. Results: isolation results obtained two isolates of Trichoderma sp. (P1 and P2), two isolates of Trichoderma sp. (P1 and P2) were able to suppress the level of pathogenic fungal infections carried by rice seeds in the incubation method using PDA media and the growing on test method, 3.75%, two isolates of Trichoderma sp. able to increase the germination capacity of rice seeds by P1 (12.50%) and P2 (31.25%) respectively. Conclusions: Two isolates of Trichoderma sp. (P1 and P2) could suppress the level of pathogenic fungal infections carried by rice seeds in the incubation method using PDA media and in the growing on test method. Apart from that, two isolates of Trichoderma sp.
EFIKASI KONSORSIUM Bacillus sp. DAN Pseudomonas fluorescens TERHADAP LAYU FUSARIUM PADA TANAMAN CABAI RAWIT (Capsicum frutescens L.) Nensi Agustina; Arika Purnawati; Endang Triwahyu Prasetyawati; Safira Rizka Lestari
Jurnal Agrotek Tropika Vol 12, No 3 (2024): JURNAL AGROTEK TROPIKA VOL 12, AGUSTUS 2024
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v12i3.5724

Abstract

Fusarium oxysporum f.sp. capsici merupakan patogen penting pada cabai rawit karena menyebabkan penyakit layu, merugikan dan menurunkan produksi hingga 50%. Pengendalian secara kimiawi menggunakan fungisida telah banyak dilakukan namun berdampak negatif terhadap lingkungan. Berdasarkan percobaan di lapangan, aplikasi konsorsium bakteri Bacillus sp. dan Pseudomonas fluorescens dapat mengendalikan penyakit layu Fusarium. Tujuan dari penelitian ini adalah untuk mengetahui tingkat efikasi dan pengaruh aplikasi konsorsium Bacillus sp. dan Pseudomonas fluorescens terhadap penyakit layu yang disebabkan oleh F. oxysporum f.sp. capsici. Penelitian ini dilakukan dengan menginokulasi 100 ml  F. oxysporum f.sp. capsici (104 spora ml-1) dan 25 ml konsorsium bakteri (109 CFU mL-1), lalu diamati tingkat efikasi dan pengaruhnya terhadap perkembangan penyakit layu Fusarium. Rancangan penelitian ini adalah Rancangan Acak Lengkap (RAL) yang terdiri dari dua faktor yaitu konsorsium Bacillus sp. (Ba9) dengan Pseudomonas fluorescens, konsorsium Bacillus sp. (Ba17) dengan Pseudomonas fluorescens, ulangan sebanyak tiga kali dan analisis data menggunakan uji Tukey's HSD. Hasil penelitian bahwa konsorsium bakteri dapat menurunkan intensitas penyakit rata-rata 45-66%.
The In Vitro Antifungal Impact of Secondary Metabolites from Trichoderma sp. on Fusarium sp. Uswatun Hasanah; Arika Purnawati; Herry Nirwanto
Jurnal Agrinika: Jurnal Agroteknologi dan Agribisnis Vol 7 No 2 (2023): SEPTEMBER
Publisher : Kadiri University - Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30737/agrinika.v7i2.4511

Abstract

Fusarium sp. is a significant pathogenic fungus responsible for causing wilt in various plants, including chilies, eggplants, and shallots. One approach to address this issue involves utilizing secondary metabolites from the fungus Trichoderma sp. These metabolites were applied at concentrations of 10%, 20%, and 30% alongside a fungicide called M-Dithane 45, following the recommended dosage specified on the packaging. This research aims to evaluate the impact of these secondary metabolites in suppressing the Fusarium sp. pathogen. The study occurred at the Plant Health Laboratory I within the Faculty of Agriculture at Universitas Pembangunan Nasional “Veteran” East Java from October to December 2022. It adopted a single-factor, Completely Randomized Design (CRD), and data analysis was carried out using R-studio software. In vitro analysis was conducted through the dual culture technique on a PDA medium, assessing inhibition percentages and observing the morphology of Fusarium sp. fungi. The results demonstrated that the highest inhibition percentage of Fusarium sp. occurred at a 30% concentration, reaching 26% compared to the control group. Morphological examinations of Fusarium sp. indicated that all treatments affected the growth of abnormal hyphae, including bending, curling, coiling, and lysis.
POTENTIAL OF ENCAPSULATION Bacillus cereus BTH-22 AGAINTS BACTERIAL WILT DISEASE ON EGGPLANT: - Purnawati, Arika; Triwahyu P, Endang; Fari A, Haidar
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.3736

Abstract

Endophytic bacteria are a group of bacteria that live in plant tissues, potentially as biological agents of plant diseases, especially wilt disease caused by Ralstonia solanacearum and as an inducer of eggplant (Solanum melongena) plant growth. One of these bacteria is Bacillus cereus BTH-22 which was isolated from healthy eggplant stems in Kediri. The purpose and formulation of the problem are to obtain an encapsulation formulation of B. cereus BTH-22 which has the potential as a biological agent against wilt disease caused by R. solanacearum and as an inducer of eggplant (S. melongena) plant growth. The novelty of the research is the encapsulation formulation of B. cereus which is applied to eggplant plants with wilt, because the application generally uses root soaking, watering to the soil surface, mixing with fertilizer, liquid and powder formulations but all are easily washed away by rain. The research method is as follows: (1) Making encapsulation formulation: 75% Na-alginate with 10% aloe vera extract (Na-1) and 75% Na-alginate, 0.875% zeolite and 0.875% sago flour (Na-2), (2) Sterile soil, inoculate R. solanacearum and prepare 1 month old plants, (3) Application of encapsulation formulation was carried out 3 days after inoculation of R. solanacearum (107CFU/mL) using a Completely Randomized Design with 3 replications. Observations were made on days 7, 14, 21, 28, 35, 42, 49 including : disease intensity, plant height, number of leaves, number of flowers. The results of the study showed: viability in the Na-1 and Na-2 treatments was higher than control at the 48th to 96th hours, disease intensity in the Na-1 and Na-2 treatments was lower than control at the 7th to 49th day, plant height in the Na-1 and Na-2 treatments was higher than control at the 14th to 49th day.
POTENSI BAKTERI ENDOFIT ASAL LAHAN BASAH KALIMANTAN SELATAN SEBAGAI AGENSIA HAYATI NEMATODA PURU AKAR (Meloidogyne sp. ) PADA TANAMAN SELEDRI (Apium graveolens L.) SECARA IN VITRO Althaf, Marcellino; Widayati, Wilujeng; Purnawati, Arika
Agros Journal of Agriculture Science Vol 24, No 2 (2022): edisi Juli
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v24i2.1929

Abstract

Seledri (Apium graveolens L.) merupakan tanaman rempah penting yang dibudidayakan di dataran tinggi. Salah satu penyakit yang dapat menurunkan produksi tanaman seledri adalah puru akar yang disebabkan oleh nematode parasit Meloidogyne sp. Pengendalian nematoda sampai dengan saat ini menggunakan pestisida yang toksik, sulit terdegradasi, dan berdampak negatif terhadap lingkungan. Upaya pengendalian biologis saat ini banyak dikembangkan, salah satunya penggunaan mikroorganisme bakteri endofit. Tujuan penelitian: mengetahui potensi bakteri endofit asal lahan basah Kalimantan Selatan sebagai agensia hayati nematode puru akar Meloidogyne sp pada tanaman seledri. Penelitian dilaksanakan dua tahap yaitu : eksplorasi bakteri endofit di lahan basah Kalimantan Selatan Bulan November 2021 dan uji vitro dilaksanakan di Laboratorium Kesehatan Tanaman Fakultas Pertanian Universitas Pembangunan Nasional “Veteran” Jawa Timur Bulan Desember 2021 menggunakan Rancangan Acak Lengkap. Uji dilakukan dengan cara 1 ml suspensi nematoda berisi ± 50 ekor juvenile 2 nematoda hasil ekstraksi dituang dalam 5 ml suspensi bakteri endofit berumur 48 jam yang dipanen menggunakan media NB dan diuji keamanan hayatinya dengan kode isolate BLR 1.2, BLR 1.3, BLR 2.1, BLR 2.2, BLR 2.3, BLR 3.2, AKL 2.1, AKL 2.2, AKL 3.1. Isolate kode BLR 2.2, BLR 1.3 ,BLR 3.2, BLR 2.1 merupakan isolate dengan jumlah mortalitas tertinggi dengan jumlah 50.67%, 45.33%, 49.33% dan 45.33% pada 12 jam setelah perlakuan
POTENSI FORMULASI BIOFUNGISIDA Bacillus sp. (Bth-22) DAN Streptomyces sp. (TMP) TERHADAP PERKECAMBAHAN BENIH JAGUNG Maharani, Salsabilla Diva; Purnawati, Arika; Suryaminarsih, Penta
Agrika Vol. 19 No. 1 (2025): MEI 2025
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v19i1.6754

Abstract

Jagung (Zea mays) adalah salah satu tanaman pangan utama yang memiliki peran penting dalam ketahanan pangan global. Fusarium sp. merupakan patogen tanah yang sering ditemukan pada lahan jagung dan dapat mengganggu perkecambahan dan pertumbuhan tanaman jagung. Penggunaan bakteri Bacillus sp. dan Streptomyces sp. sebagai bahan aktif biofungisida dapat meningkatkan persentase perkecambahan benih dan pertumbuhan tanaman jagung yang terserang patogen Fusarium sp.. Penelitian ini bertujuan untuk mengetahui pengaruh jenis media dan potensi formulasi biofungisida dengan bahan aktif Bacillus sp. dan Streptomyces sp. dalam menunjang perkecambahan dan pertumbuhan tanaman jagung. Penelitian dilakukan dengan Rancangan Acak Lengkap (RAL) dengan dua faktor. Faktor pertama yakni media produksi bakteri: K: Air rebusan kedelai, E: Estrak Kentang Gula (EKG), dan A: Air kelapa. Faktor kedua adalah perbandingan formulasi Bacillus sp. dan Streptomyces sp. yang terdiri atas 4 taraf yaitu BS0 (0:0), BS1 (3:3), BS2 (2:4), dan BS3 (1:5). Hasil penelitian menunjukkan tidak terdapat interaksi antara kedua faktor perlakuan. Perlakuan BS2 merupakan perlakuan terbaik yang memberikan presentase perkecambahan sebesar 82%, hari berkecambah 3.64 HST, jumlah daun pada 35 HST sebanyak 5.7 helai dan 62.8 cm.
The Application of Organic Plant Media Composition on Indian Mustard Crops (Brassica Juncea L) in Polybag Augustien, Nora; Indah, Pawana Nur; Arika , Purnawati; Irsyad, Irsyad; Suhardjono , Hadi
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 1 No. 1 (2017)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v1i1.3

Abstract

Indian mustard plants are the main vegetable crops consumed by urban communities. The need for this vegetable increases along with the increase in culinary tourism in each region and the government's appeal on sustainable food home. One effort made to meet the needs of vegetable mustard in urban areas can be cultivated using polybags. The main problem of vegetable cultivation on polybags is the availability of soil media. To overcome the limitations of soil media it is attempted to substitute it with organic litter enriched with MOL (local microorganisms). The purpose of research is to find the right combination of planting media for mustard plants. The results showed that the composition of K7 = soil: compost: litter of hay: litter of maize (2: 1: 1: 1) best on plant length, number of leaves, length of root leaves and number of roots while the wet weight of mustard plant (g) K7 = soil: compost: litter of maize: litter of corn (2: 1: 1: 1) and K8 = soil: compost: litter of maize: litter of maize: cocopeat (2: 1: 1: 1: 1) or soil: compost + urea. Increased weights of wet mustard by 63% compared to soil media and 20% compared to soil composition: compost + urea. Organic straw in the form of straw: litter of corn stalk: cocopeat enriched with MOL are able to become ready-made planting media on the cultivation of indian mustard plants in polybags.
Antagonistic Test of Bacillus spp. against Fusarium sp., the Causal Agent of Wilt Disease of Red Chili Plants Heriyati, Sukma; Prasetyawati, Endang Triwahyu; Purnawati, Arika
CROPSAVER Vol 6, No 1 (2023)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v6i1.45386

Abstract

Wilt disease caused by Fusarium oxysporum is one of the diseases affecting chili plants. The utilization of Bacillus spp. can be a solution for plant disease control because it can colonize plants and produce useful microbe compounds to inhibit the development of plant pathogen. This study aimed to determine the ability of Bacillus spp. to suppress the growth of Fusarium sp. isolates from chili plants in vitro. Bacillus sp. isolates Ba-6, Ba-9, Ba-12, Ba-15, Ba-17, and a control (aquadest) were used as treatments, each replicated four times. The observation parameters were the inhibition zone test and microscopic observations of the morphology of Fusarium sp. after the inhibition zone test. The highest inhibition was shown in the treatment of Bacillus sp. isolate Ba-15, which was 20.02%. The observation of Fusarium sp. hyphae after the inhibition zone test showed abnormal growth, which was different for each isolate. Some hyphae were bent, coiled, shrunken, swollen, curled, or lysed.
Metabolit Sekunder Bacillus sp. sebagai Antijamur Jamur Patogen Tular Benih pada Benih Jagung dengan Metode Uji Blotter Setyowati, Laras; Purnawati, Arika; Mujoko , Tri; Mukaromah, Fathul
BIOEDUSCIENCE Vol 8 No 1 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12588

Abstract

Background: Seed is a planting material that must have high viability and quality, including being free from pathogens. One of the obstacles in the supply of quality maize seeds is seed-borne fungal pathogen which can affect the quality of maize seeds and cause plant disease. Endophytic bacteria Bacillus sp. has been widely studied to be able to produce secondary metabolites as antifungals. This study aimed to determine the exact concentration of secondary metabolites of endophytic bacteria Bacillus sp. to decrease the infections of seed-borne pathogens fungal on maize seeds using the blotter test method. Methods: This study used a completely randomized design with four concentration levels (10%, 15%, 20%, 25%) and two strain codes of endophytic bacteria Bacillus sp. (Bth-31a and Bth-22). Results: The result of identifying seed-borne fungal pathogen on maize seed included Fusarium sp., Aspergillus flavus, Aspergillus niger, and Rhizopus sp. The Bth-22 treatment with a concentration of 25% could decrease the infection of seed-borne fungal pathogen and had the highest percentage until 50% compared to the control. While the lowest percentage to decrease the infections of seed-borne fungal pathogen occurred in the Bth-31a treatment with a concentration of 10%, which was 32.1% compared to the control. Conclusions: All seed treatments that used secondary metabolites of Bacillus sp. able to decrease the infections of seed-borne fungal pathogen on maize seed compared to the control. In addition, the treatment of secondary metabolites Bacillus sp. is able to increase the germination of maize seeds.
POTENTIAL OF ENCAPSULATION Bacillus cereus BTH-22 AGAINTS BACTERIAL WILT DISEASE ON EGGPLANT: - Purnawati, Arika; Triwahyu P, Endang; Fari A, Haidar
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.3736

Abstract

Endophytic bacteria are a group of bacteria that live in plant tissues, potentially as biological agents of plant diseases, especially wilt disease caused by Ralstonia solanacearum and as an inducer of eggplant (Solanum melongena) plant growth. One of these bacteria is Bacillus cereus BTH-22 which was isolated from healthy eggplant stems in Kediri. The purpose and formulation of the problem are to obtain an encapsulation formulation of B. cereus BTH-22 which has the potential as a biological agent against wilt disease caused by R. solanacearum and as an inducer of eggplant (S. melongena) plant growth. The novelty of the research is the encapsulation formulation of B. cereus which is applied to eggplant plants with wilt, because the application generally uses root soaking, watering to the soil surface, mixing with fertilizer, liquid and powder formulations but all are easily washed away by rain. The research method is as follows: (1) Making encapsulation formulation: 75% Na-alginate with 10% aloe vera extract (Na-1) and 75% Na-alginate, 0.875% zeolite and 0.875% sago flour (Na-2), (2) Sterile soil, inoculate R. solanacearum and prepare 1 month old plants, (3) Application of encapsulation formulation was carried out 3 days after inoculation of R. solanacearum (107CFU/mL) using a Completely Randomized Design with 3 replications. Observations were made on days 7, 14, 21, 28, 35, 42, 49 including : disease intensity, plant height, number of leaves, number of flowers. The results of the study showed: viability in the Na-1 and Na-2 treatments was higher than control at the 48th to 96th hours, disease intensity in the Na-1 and Na-2 treatments was lower than control at the 7th to 49th day, plant height in the Na-1 and Na-2 treatments was higher than control at the 14th to 49th day.
Co-Authors . NUGROHORINI . WAGIYANA Ahmad Adibul Akrom Ahmad Davin Najwan Adhyasta Akrom, Ahmad Adibul Al Rahmad, Nirmala Putri Alfa, Maria Rodhya Althaf, Marcellino Alvia Cindy Apriliani Amalia, Anisa Rizki Aris Cahyono Arkan Haykal Augustien, Nora Bambang HADISUTRISNO Diasi, Ananta Rizki Didik Sulistyanto Dwirizki Permata, Aprilla Hendiana Elly Syafriani Fari A, Haidar Fatimah Nabila Zahra Firmansyah, Moch Ryan Hadi Suhardjono Haidar Fari Aditya Hasni Wardahni Heriyati, Sukma Herry Nirwanto Herry Nirwanto Iis Purnamawati Indah Pemuda Indah, Pawana Nur Indarwati Indarwati Irsyad Irsyad Irsyad, Irsyad irsyad, irsyad Ketut Srie Marhaeni Julyasih Khairunnisa, Nabila Zahra Kurniawati Kurniawati Kusumawati, Diah Budi Laras Setyowati Laras Setyowati Maharani, Salsabilla Diva Marcellino Althaf Maria Rodhya Alfa Maulidya, Aisyah Maulina Aizah, Shinta Muhammad Raihan Naufal Mujoko , Tri Mukaromah, Fathul Nensi Agustina Nensi Agustina Nensi Agustina Nijma Nurfadila Noni Rahmadhini Nora Augustien Novi Nafisah Najmi Nur Aini Mahfud Nur Aini Mahfud Nurul Afida Nurul Laily Pawana Nur Indah Penta Suryaminarsih Penta SURYAMINARSIH Penta Suryaminarsih Prasetyawati, Endang Triwahyu Prasetyowati, Endang Triwahyu Presti Mardiyani Pribadi, Didik Utomo RAMADHANI MAHENDRA KUSUMA Rizky Ika Noviyanti Rizky Ika Noviyanti Saefur Rohman Safira Rizka Lestari Satrio Hanif Haryawan Sayekti, Ninik Ari Setyowati, Laras Sri Wiyatiningsih Sri Wiyatiningsih Sri Wiyatiningsih Suhardjono , Hadi Syamsul Arifin Tri Mudjoko Tri MUJOKO Triwahyu P, Endang Triwidodo ARWIYANTO USWATUN HASANAH Wafiah Huwaina Zata Dini Widayati, Wilujeng Widyananda, Kanaya Syafina Wilujeng Widayati Windriyanti, Wiwin Wiwik Harjani Yanti, Vika Yenny WURYANDARI Yenny Wuryandari Yerni Andryani Yuningsih Yuningsih Zakaria Wildan Fachrezzy Zakqy, Nauval