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All Journal AGRIVITA, Journal of Agricultural Science Jurnal Penelitian Pertanian Terapan Jurnal Agrotek Tropika Klorofil: Jurnal Penelitian Ilmu-Ilmu Pertanian Jurnal Abdimas Mandiri Jurnal Pengabdian Masyarakat : Pemberdayaan, Inovasi dan Perubahan Jurnal Pengabdian Kepada Masyarakat (Abdimas) Universitas Baturaja
Ekawati Danial
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Journal : AGRIVITA, Journal of Agricultural Science

 1 
IN VITRO SHOOT REGENERATION OF INDONESIAN BANANAS (Musa spp.) cv. AMBON KUNING AND RAJA BULU, PLANTLET ACCLIMATIZATION AND FIELD PERFORMANCE Yusnita, Yusnita; Danial, Ekawati; Hapsoro, Dwi
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of this study was to observe responses of two banana cultivars ‘Ambon’ and ‘Raja Bulu’ on different BA concentrations and effects of different media and fertilizer on survival and growth of plantlets.  Sterilized explants were cultured on initiation medium (MS with 1mg L-1 BA) for 4 weeks, then subjected to media MS with 2.5, 5.0, or 7.5 mg L-1 BA. Numbers of shoot buds, shoots and propagules were recorded after 4 consecutive passages with 4 weeks intervals.  Rooted plantlets were acclimatized in three different media, then treated with or without NPK (32:10:10) fertilizer solution once a week. After 2 months, the survival and growth of plantlets were recorded. Cultures of banana ‘Ambon Kuning’ showed higher regenerative capacity compared to ‘Raja Bulu’, producing higher numbers of shoot buds, shoots and propagules. The best medium for propagule proliferation of both banana cultivars was MS+5 mg L-1 BA, producing 40.7 propagules for ‘Ambon Kuning’, and 12.3 propagules for ‘Raja Bulu’ per explant. In all acclimatization media tested, 100% of plantlet survival was achieved. The best plantlet growth was found in sand: compost (1:1,v/v) with application of NPK solutions. The in vitro-derived plants were planted in the field and produced fruits of high quality.Keywords: acclimatization, benzyladenine, fertili-zer, genotypes, media mixtures
IN VITRO SHOOT REGENERATION OF INDONESIAN BANANAS (Musa spp.) cv. AMBON KUNING AND RAJA BULU, PLANTLET ACCLIMATIZATIONAND FIELD PERFORMANCE Yusnita, Yusnita; Danial, Ekawati; Hapsoro, Dwi
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i1.438

Abstract

The aim of this study was to observe responses of two banana cultivars ‘Ambon’ and ‘Raja Bulu’ on different BA concentrations and effects of different media and fertilizer on survival and growth of plantlets.  Sterilized explants were cultured on initiation medium (MS with 1mg L-1 BA) for 4 weeks, then subjected to media MS with 2.5, 5.0, or 7.5 mg L-1 BA. Numbers of shoot buds, shoots and propagules were recorded after 4 consecutive passages with 4 weeks intervals.  Rooted plantlets were acclimatized in three different media, then treated with or without NPK (32:10:10) fertilizer solution once a week. After 2 months, the survival and growth of plantlets were recorded. Cultures of banana ‘Ambon Kuning’ showed higher regenerative capacity compared to ‘Raja Bulu’, producing higher numbers of shoot buds, shoots and propagules. The best medium for propagule proliferation of both banana cultivars was MS+5 mg L-1 BA, producing 40.7 propagules for ‘Ambon Kuning’, and 12.3 propagules for ‘Raja Bulu’ per explant. In all acclimatization media tested, 100% of plantlet survival was achieved. The best plantlet growth was found in sand: compost (1:1,v/v) with application of NPK solutions. The in vitro-derived plants were planted in the field and produced fruits of high quality.
Co-Authors Albetry, Albetry Ariyadi, Rahmat Asroh, Ardi Dwi Hapsoro DWI HAPSORO Eka Rizki Meiwinda Enda Kartika Sari, Enda Kartika Endang Lastinawati, Endang Fatmanurshanti, Dora Gribaldi Gribaldi Irawan, Ahmad Dieky Joko Kuswanto Lestari, Windi Novriani, Novriani Novriani, Novriani Nurlaili Nurlaili Nurlaili Nurlaili Nurmala Dewi, Nurmala Nurshanti, Dora Fatma Nurshanti, Dora Fatma Oktarina, Yetty Purwadi Purwadi Pusvita, Ema Putri Ayu Ogari, Putri Ayu Putri, Piranti Herdaning Putri, R. A. HayuSriwijaya Rahma, Sindi Siti Rosmawati, Henny Sakalena, Firnawati Saputra, Doris Sebayang, Nico Septianita, Septianita Siti Muyaroah Susanti - Diana SUSANTI DIANA Utari, Sindy Tria Yasa, Adiran Putra Yulhasmir, Yulhasmir Yusnita Yusnita YUSNITA YUSNITA Zairani, Fitri Yetty