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All Journal HAYATI Journal of Biosciences SEMIRATA 2015 Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Biotechnology Majalah Obat Tradisional Journal of Tropical Biodiversity and Biotechnology Jurnal Kimia Riset Indonesian Journal of Chemistry Biosaintifika: Journal of Biology & Biology Education Cakrawala: Jurnal Litbang Kebijakan Darmabakti Cendekia: Journal of Community Service and Engagements Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Bio-molecule Research And Engineering Andan Jejama: Indonesian Journal Of Community Engagement
Alfinda Novi Kristanti
Departemen Kimia, Fakultas Sains Dan Teknologi, Universitas Airlangga.
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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PENDAMPINGAN MERDEKA BELAJAR BIDANG KIMIA KEPADA GURU DAN SISWA SMA DI KOTAWARINGIN BARAT – KALIMANTAN TENGAH Nanik Siti Aminah; Alfinda Novi Kristanti; Rico Ramadhan; Andika Pramudya Wardana; Tio Christiawan Bramayudha
Jurnal Layanan Masyarakat Vol. 6 No. 2 (2022): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v6i2.2022.492-499

Abstract

Kabupaten Kotawaringin Barat, Provinsi Kalimantan Tengah memiliki penduduk yang didominasi oleh usia muda/dewasa dan pemerintah daerah selalu berupaya meningkatkan kualitas SDM sebagai salah satu kiat menuju penguatan ekonomi yang merupakan fokus perhatian pemerintah. Departemen Kimia Universitas Airlangga mendukung usaha pemerintah daerah dengan menyelenggarakan kegiatan pengabdian masyarakat kepada guru SMA bidang Kimia dan siswa SMA. Kegiatan dilaksanakan pada 22 – 23 Agustus 2022 dan meliputi 3 program utama. Pertama, kegiatan pelatihan skrining bioaktivitas ekstrak tanaman dan penggunaan aplikasi Chemdraw sebagai platform pembelajaran siswa diperuntukkan kepada guru SMA sederajat. Kedua, kegiatan sosialisasi pendidikan sarjana dan demonstrasi praktikum kimia ditargetkan kepada siswa SMA sederajat. Kedua kegiatan ini diselenggarakan di SMAN 1 Pangkalan Bun, Kabupaten Kotawaringin Barat. Ketiga, international community service yang dihadiri melalui zoom meeting oleh mahasiswa internasional dan terintegrasi dengan program pengmas untuk siswa SMA. Rangkaian kegiatan ini diharapkan dapat membuka wawasan guru dan siswa SMA dalam mengimplementasikan merdeka belajar. Merdeka belajar merupakan pendekatan yang dilakukan agar siswa dapat mengoptimalkan bakat dan minat guna memberikan sumbangsih terbaik bagi bangsa.
Effect of Carbon Source Variations on Growth, Physiological Stress, and Saponin Levels of Talinum paniculatum Gaertn. Adventitious Roots Nindi Novia Erin; Arif Yachya; Alfinda Novi Kristanti; Djarot Sugiarso; Yosephine Sri Wulan Manuhara
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 3 (2022): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.69359

Abstract

Monosaccharide and disaccharide as carbon sources can affect the production of secondary metabolites. The study aims to determine the effect of variations in carbon sources on growth, physiological stress, and saponin levels of the adventitious roots of Talinum paniculatum Gaerthn. Adventitious roots are subculture in liquid MS medium treated with various sugars: 3% sucrose, 3% glucose, 3% fructose, 3% lactose, 3% maltose, 3% dextrose, sucrose + fructose (1.5% + 1.5%), sucrose + glucose (1.5% + 1.5%), glucose + fructose (1.5% + 1.5%), sucrose + dextrose (1.5% + 1.5%) for 6 weeks. The results of this study show that the 3% fructose treatment produces the highest fresh and dry biomass, which are 1.30 g and 0.23 g compared to the control. The morphology of adventitious roots in the treatment of carbon source variation is not different from the control treatment. The highest MDA (malondialdehyde) levels are found in the sucrose + fructose treatment (1.5% + 1.5%). Meanwhile, the highest proline levels are found in the 3% maltose treatment. Saponin levels analyzed using thin layer chromatography show the data in the form of color intensity and stain area based on ImageJ software analysis. The 3% fructose treatment shows the highest color intensity and stain area compared to the control. Variations in carbon sources affect physiological stress, biomass, and saponin levels of adventitious roots of T. paniculatum, but do not effect on root morphology.
Evaluation of Flavonoid Content from Shoot Culture of Gynura procumbens in Balloon-type Bubble Bioreactors Ira Nailas Sa'adah; Yosephine Sri Wulan Manuhara; Alfinda Novi Kristanti
Majalah Obat Tradisional Vol 27, No 2 (2022)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.72734

Abstract

Gynura procumbens has been widely used to treat several health disorders, such as hyperglycemia, hypertension, diabetes, and allergies. This study aimed to determine the flavonoid content of G. procumbens cultured in a balloon-type bubble bioreactor using various sucrose concentrations (10, 30, and 50 g/L) and explant densities (5, 10, and 15 explants). The plant parts used were stem nodes of G. procumbens, grown in a balloon-type bubble bioreactor using Murashige and Skoog (MS) liquid media with IAA 2 mg/L and BAP 4 mg/L. The total flavonoid content was measured using a UV-Vis spectrophotometer (λ= 510 nm), and the flavonoid profile was tested using thin-layer chromatography (TLC). The administration of 10 g/L sucrose produced the highest total flavonoid production. In the treatment of explant density, the most increasing total flavonoid production was obtained in the treatment of 5 explants. The TLC results showed that each treatment forms spots like standard. This study proved that sucrose and explant densities culture on balloon-type bubble bioreactor method had optimized the production of flavonoids of plant shoots of G. procumbens culture.
ISOLATION AND STRUCTURE ELUCIDATION OF SECONDARY METABOLITE COMPOUNDS FROM Curcuma aeruginosa Alsya Firdausi Nuzula; Alfinda Novi Kristanti; Nanik Siti Aminah; Yoshiaki Takaya; Marisa Nur Sahadatin; Andika Pramudya Wardana
Jurnal Kimia Riset Vol. 8 No. 1 (2023): June
Publisher : Universitas Airlangga, Campus C Mulyorejo, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkr.v8i1.44073

Abstract

Black turmeric (Curcuma aeruginosa) is Indonesian medicinal plant belonging to the Zingeberaceae family, mostly found on Java. This study aimed to determine the new molecular structures of the compounds isolated from black turmeric originating from Indonesia. Isolation of the secondary metabolite compounds was initiated by solid-liquid extraction using the maceration method with ethanol solvent, followed by liquid-liquid extraction using the partition method with n-hexane and dichloromethane as solvents. Separation and purification were carried out using vacuum liquid chromatography (VLC) and gravity column chromatography (GCC). The isolated compounds were characterized using spectroscopic methods, including UV-Vis, IR, and NMR spectroscopy. The isolation resulted in three sesquiterpenoid compounds, (E)-7-isopropyl-4,10-dimethylcyclodec-10-ene-5,8-dione (curdione), (1R,10R)-Epoxy-(-)-1,10-dihydrocurdione, and zedoalactone B, together with two common terpenes, ethyl tetradecanoate and 1-hexadecene.
A novel pilot bioreactor for scaling up biomass and bioactive compounds on Gynura procumbens adventitious root culture Dannis Yuda Kusuma; Alfinda Novi Kristanti; Yosephine Sri Wulan Manuhara
Indonesian Journal of Biotechnology Vol 28, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.78420

Abstract

Bioreactors for adventitious root culture have been developed to obtain biomass and plant bioactive compounds in large quantities. These technologies provide a great opportunity to produce biomass and bioactive compounds more quickly from Gynura procumbens compared to conventional plant cultivation systems. In previous studies, biomass and bioactive compounds of G. procumbens adventitious roots were successfully increased using a small‐scale bioreactor. In this study, a pilot bioreactor the capacity of 19 L polycarbonate gallon was successfully developed. This bioreactor can be sterilized under the pressure of 0.18 MPa for approximately 60 min. While the bioreactor could not be sterilized when the pressure was less than 0.18 MPa damage may have occurred to the bioreactor vessel at pressures exceeding 0.18 MPa. The results of the chemical grade test as root culture media showed that MS‐Tek provided an optimal root biomass compared to MS‐PA after a 35‐day of the culture period. In addition, the productivity of the total phenolics and flavonoids of adventitious root in MS‐PA was higher than in MS‐Tek. This novel pilot bioreactor is suitable for G. procumbens adventitious root culture, and the technical‐grade chemicals are suitable for improving root biomass production.
Optimization of Biomass and Secondary Metabolite Production in Gynura procumbens (Lour.) Merr. Adventitious Roots Culture by Using the Method of Subculture and Fed-batch Cultivation in a Bioreactor Dannis Yuda Kusuma; Alfinda Novi Kristanti; Anjar Tri Wibowo; Boon Chin Tan; Yosephine Sri Wulan Manuhara
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.797-807

Abstract

The valuable extract of bioactive compounds from Gynura procumbens has been widely manufactured into various health products. The demand for these compounds is continuously increasing, but production through conventional farming methods is insufficient due to limited agricultural land and environmental stresses. An alternative to producing plant biomass is in vitro cultivation methods. This method requires less space and enables biomass propagation in a controlled condition that can facilitate stable and efficient production of plant secondary metabolites. This study evaluated the effect of inoculum subculture periods and culture methods on G. procumbens biomass and secondary metabolite production in a bioreactor. The 3-L airlift balloon type-bubble bioreactors was modified in this study to adopt the treatment of 1st-5th subculture periods and fed- and batch-cultivation strategies. We found the G. procumbens adventitious root culture was optimally derived from the 1st subculture produced biomass of 148.02±1.45 g FW and 8.59±0.12 g DW, and TPC (14.48±1.08 mg GAE/g DW) and TFC (116.89±0.44 mg KE/g DW and 33.97±0.13 mg QE/g DW). Additionally, the fed method after 28 days of culture using double distilled water replenishment improved adventitious root biomass (213.75±35.00 g FW and 11.21±0.18 g DW), while nutrient replenishment improved TFC (52.14±0.44 mg KE/g DW and 14.54±0.13 mg QE/g DW). These results can be used to optimize the cultivation of G. procumbens adventitious roots in a large-scale bioreactor.
Biomass Production of Gynura procumbens Adventitious Roots in Different Type of Liquid Culture Yosephine Sri Wulan Manuhara; Dannis Yuda Kusuma; Rafika Lailiyatul Kurnia Sari; Alfinda Novi Kristanti
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.9670

Abstract

Gynura procumbens has a potency to produce raw material for pharmaceutical industry. Liquid culture systems have significant effects on multiplication rates of organ, so the propagation technology to increase biomass of this plant or organ using the liquid culture system was necessary. This research was conducted to compare biomass production of adventitious roots of G. procumbens in different liquid culture (shake flask, temporary immersion bioreactor and balloon-type bubble bioreactor). Adventitious roots culture was maintained in Murashige and Skoog liquid medium supplemented with IBA (indole butyric acid) 5 mg/L and different concentrations of sucrose (10, 30, 50 g/L). Result showed that the highest biomass production (fresh weight) was in shake flask that was 3.9-fold higher than initial explant, while in temporary immersion system was 5.12-fold higher than initial explant, and in balloon- type bubble bioreactor was 13.1-fold higher than initial explant. The highest enhancement of adventitious roots was occurred at sucrose supplementation of 50 g/L. Based on this research we found that the best type of liquid culture to increase biomass of G. procumbens adventitious roots was balloon-type bubble bioreactor. Scaling-up of adventitious root culture are necessary to fulfill the need of raw material for pharmaceutical industry and ballon-type bubble bioreactor was the most suitable method to scale up the performance. 
THE PRELIMINARY STUDY OF ANTIOXIDANT ACTIVITY FROM XYLO-OLIGOSACCHARIDE OF CORNCOB (ZEA MAYS) HYDROLYSIS PRODUCT WITH ENDO-β-XYLANASE ENZYME Yamani, Laura Navika; Kristanti, Alfinda Novi; Puspaningsih, Ni Nyoman
Indonesian Journal of Tropical and Infectious Disease Vol. 3 No. 2 (2012)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.817 KB) | DOI: 10.20473/ijtid.v3i2.219

Abstract

Xylo-oligosaccharide derived from corncob hemicellulose has been reported to possess antioxidant activity. In order to assess the effective scavenging of xylo-oligosaccharide, we conducted in vitro studies based on self-made xylo-oligosaccharide with DPPH (2,2diphenyl-1-picrilhydrazil) method. Xylo-oligosaccharide was prepared with enzymatic hydrolysis. The enzyme used for hemicellulose hydrolysis was endo-β-xylanase enzyme from PC-01 isolated bactrerium. PC-01 isolated bacterium used in this study was Pacet hot spring which was isolated from East Java. Endo-β-xylanase enzyme is an extracelluler enzyme. There was about 0.199 U/mL after purification and dialysis process. Hydrolisis product of hemicellulose A and B from corncob were analyzed with TLC (Thin Layer Chromatography) and HPLC (High Performance Liquid Chromatography). This analysis showed that hydrolysis product of hemicellulose B had a lot of xylo-oligosaccharide hydrolysis product of hemicellulose than Xylo-oligosaccharide hydrolysis product of hemicelluloses A. Xylo-olygosaccharide was analyzed as on antioxidant activity. Xylo-oligosaccharide hydrolysis product ofhemicellulose B (IC = 48.96) has higher antioxidant activity than Xylo-oligosaccharide hydrolysis product of hemicellulose A (IC 50 50 = 92.302). The toxicity of xylo-oligosaccharide can be calculated by the value of LC 50 (Lethality concentration). LC of xylooligosaccharide derived from corncob hemicellulose was 400 ppm so that xylo-oligosaccharide has anti tumor activity because xylooligosaccharide has LC 50 < 1000 ppm.
Methyl Jasmonate Elicitation Enhanced Biomass, Phenolic, and Flavonoid Production of Adventitious Root Culture of Gynura procumbens in Balloon Type Bubble Bioreactor Wijayanti, Ely Tri; Kusuma, Dannis Yuda; Kristanti, Alfinda Novi; Wibowo, Anjar Tri; Sugiharto, Sugiharto; Sugiarso, Djarot; Manuhara, Yosephine Sri Wulan
Majalah Obat Tradisional Vol 29, No 2 (2024)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.87736

Abstract

Secondary metabolites in the medicinal plant Gynura procumbens have various beneficial pharmacological activities. However, the limited availability of these beneficial compounds has constrained the possibilities for therapeutic applications. Exogenously applied methyl jasmonate (MeJa) as an elicitor has been reported to trigger the accumulation of secondary metabolites in plant tissues cultured in vitro. This study aimed to assess how varying periods of MeJa elicitation affect the growth and accumulation of phenolic and flavonoid compounds in adventitious root cultures of G. procumbens within a balloon-type bubble bioreactor (BTBB). Adventitious roots were induced from leaf and stem explants obtained from established in vitro shoots. Upon transfer to the BTBB, the cultures were supplemented with 100 µM MeJa and sampled at 1, 2, 3, and 4 weeks. The results revealed that including MeJa in the culture medium significantly boosted growth and the accumulation of phenolic and flavonoid compounds in G. procumbens. The highest biomass and concentrations of phenolic and flavonoid compounds were observed after 3 weeks of elicitation. These findings highlight the potential of MeJa elicitation for enhancing the production of valuable bioactive compounds in G. procumbens adventitious root cultures, showcasing its utility for industrial applications.
Evaluation of Temperature Stress Under Different Hydroponic Systems on Growth and Saponin Content of Talinum paniculatum Gaertn. Cuttings Yachya, Arif; Kristanti, Alfinda Novi; Manuhara, Yosephine Sri Wulan
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.84241

Abstract

Increases in the temperature of nutrient solutions have restricted the use of hydroponic cultivation in the tropics, predominantly due to plant stress. This study aimed to evaluate the effects of temperature stress under different hydroponic systems on the growth and saponin content of Talinum paniculatum cuttings. Three hydroponic systems, i.e., deep flow technique (DFT), nutrient film technique (NFT), and aeroponic, were tested. The temperature of the nutrient solution was set for each system, i.e., under ambient temperature (UAT) and with controlled temperature (WCT) at 26° C. The cultivation period was 60 days. The result showed peroxidation activity and proline accumulation for the adventitious roots of T. paniculatum cuttings with UAT and WCT, alongside various levels of plasma membrane damage. Levels of Malondialdehyde (MDA) and proline were analyzed by spectrophotometer. Membrane damage was analyzed with Evans blue dye. The results indicated that the levels of MDA and proline accumulation under the three hydroponic systems were higher for the WCT than for the UAT treatment. In contrast, vegetative growth was higher in UAT than in WCT. The saponin content of the adventitious root correlated with the MDA level. Saponin production was triggered by oxidative stress during cultivation, while the adventitious roots had a higher saponin content in all three hydroponic systems with the WCT treatment compared to the UAT treatment. Among the systems, aeroponic was superior for biomass and saponin. Root growth was promoted in the nutrient solution under ambient temperature whereas the production of saponins was stimulated under the controlled temperature. In the aeroponic system, root biomass values of 1.17 and 0.478 g dry weight were obtained under ambient and controlled temperatures, respectively. The total saponin contents differed slightly, namely 189.83 and 195.61 mg/g, respectively. 
Co-Authors Abdjan, Muhammad Ikhlas Alfa Akustia Widati Alfian, Fariz Rizky Alsya Firdausi Nuzula Aminy, Aisyah Ana Firdausyah Andika Pramudya Wardana Andika Pramudya Wardana Anik Fathur Rohmah Aning Purwaningsih Anjar Tri Wibowo Antonius Budi Prasetyo Ardi Kurniawan Arga Wal Yudha Arif Yachya Baharin, Siti Nor Atika Boon Chin Tan Choirotul Isma Citra Putri Pramitha Dannis Yuda Kusuma Dannis Yuda Kusuma Djarot Sugiarso Djarot Sugiarso Fatmawati Fatmawati Haq, Kautsar Ul Hariyanto, Geraldo Figo Hartati Hartati Herdianto, Muhammad Hendra Hery Suwito Imam Siswanto Ira Nailas Sa&#039;adah Khoso, Mir Hasan Kusuma, Dannis Yuda Kusuma, Dannis Yuda Laura Navika Yamani Marisa Nur Sahadatin Mochamad Zakki Fahmi Nanik Siti Aminah Ni Nyoman Puspaningsih, Ni Nyoman Nindi Novia Erin Nur Chamidah Qonitah Labibah R. Djarot Sugiarso Rafika Lailiyatul Kurnia Sari Rafika Lailiyatul Kurnia Sari Rico Ramadhan, Rico Rimuljo Hendradi Riska Surya Ningrum Rusdipoetra, R. Aryabraga S. Sugiharto Siregar, Naufal Ramadhan Al Akhwal Sugiharto Suhaili, Suhaili Syarifah Asyura, Syarifah Takaya, Yoshiaki Tio Christiawan Bramayudha Wardana, Andika Pramudya Wijayanti, Ely Tri Wulandari, Nuryuliana Yaung Kwee Yohanes Kartjito Putro Yosephine Sri Wulan Manuhara Yoshiaki Takaya Yoshiaki Takaya