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All Journal HAYATI Journal of Biosciences Biotika: Jurnal Ilmiah Biologi Indonesian Journal of Pharmaceutical Science and Technology Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Kultivasi Jurnal Biodjati Chimica et Natura Acta Indonesian Journal of Chemistry Jurnal Mikologi Indonesia Biosaintifika: Journal of Biology & Biology Education JURNAL SELULOSA 3BIO: Journal of Biological Science, Technology and Management Proceeding Biology Education Conference Indonesian Journal of Medical Sciences and Public Health Jurnal Sylva Lestari Makara Journal of Science Journal of Fibers and Polymer Composites Molekul: Jurnal Ilmiah Kimia

Asri Peni Wulandari

Laboratorium Mikrobiologi, Departemen Biologi, FMIPA, Universitas Padjadjaran

Author-ID : 557931

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Veterinary

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Green Synthesis Nanopartikel Perak dengan Bioreduktor Ekstrak Daun Rami (Boehmeria nivea) Melalui Iradiasi Microwave Victor Handoko; Afina Yusradinan; Annas Nursyahid; Ayu Wandira; Asri Peni Wulandari
Chimica et Natura Acta Vol 10, No 1 (2022)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v10.n1.35755

Nanopartikel perak dikategorikan sebagai material antibakteri karena kemampuannya merusak integritas membran sel bakteri. Terdapat berbagai metode untuk menyintesis nanopartikel perak, salah satunya adalah dengan pendekatan green synthesis menggunakan bioreduktor ekstrak tanaman. Daun rami (Boehmeria nivea) mengandung senyawa aktif yang bersifat antibakteri dan antiviral seperti etil palmitat, 1-(4-bromobutil)-2-piperidinon, dan α-metil linoleat. Selain itu ekstrak daun rami berpotensi mereduksi ion perak menjadi nanopartikel perak karena mengandung senyawa dietilen glikol, asam miristoleat dan tetradeka-13-en-11-un-1-ol. Proses reduksi ion perak dapat dioptimalkan dengan iradiasi microwave melalui pemanasan yang seragam dan cepat. Penelitian ini bertujuan untuk melakukan green synthesis dan karakterisasi nanopartikel perak dengan ekstrak daun rami melalui iradiasi microwave. Tahapan penelitian ini terdiri dari pembuatan ekstrak daun rami, sintesis nanopartikel perak dengan prekursor perak nitrat, dan karakterisasi dengan menggunakan spektrofotometer UV-Vis, FT-IR, PSA dan XRD. Hasil pengukuran dengan spektrofotometer UV-Vis menunjukkan adanya serapan pada panjang gelombang 444 nm yang mengonfirmasi terbentuknya nanopartikel perak. Berdasarkan hasil PSA dan SEM diketahui nanopartikel perak terbentuk di antara sejumlah aglomerat. Hasil FT-IR menunjukkan adanya peran senyawa fenolik dari ekstrak dalam mereduksi prekursor perak nitrat, sedangkan hasil XRD menunjukkan kristal berbentuk kubus berpusat muka dengan rata-rata ukuran krsital sebesar 18,80 nm.

Skrining Aktivitas Antibakteri dari Fungi Simbion Karang Lunak Sarcophyton sp. Hasil Isolasi di Perairan Pulau Pramuka, Taman Nasional Laut Kepulauan Seribu Asri Peni Wulandari; Nidia Primastia
Jurnal Mikologi Indonesia Vol 6, No 1 (2022): Juni 2022
Publisher : Perhimpunan Mikologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46638/jmi.v6i1.184

Fungi yang bersimbiosis dengan karang lunak Sarcophyton sp. telah diisolasi dari Perairan Pulau Pramuka, Taman Nasional Laut Kepulauan Seribu dan diperoleh sebanyak enam isolat. Penelitian ini bertujuan untuk melakukan identifikasi secara makroskopis terhadap isolat fungi dari Sarcophyton sp. serta melakukan uji antibakteri terhadap Escherichia coli dan Staphylococcus aureus. Konsentrasi hambat minimum ditentukan dengan metode dilusi cair. Hasil penelitian menunjukkan isolat fungus dengan kode FSarc-04 memiliki aktivitas antibakteri terbaik terhadap dua bakteri standar uji dengan nilai konsentrasi hambat minimum (KHM) terhadap bakteri E. coli ATCC 25922 adalah 150 mg L-1, sedangkan E. coli tipe liar 300 mg L-1. Nilai KHM untuk S. aureus ATCC 25923 adalah 300 mg L-1, dan S. aureus tipe liar 600 mg L-1. Hasil penelitian ini menunjukkan potensi isolat fungi FSarc-04 dapat dikembangkan sebagai kandidat obat anti diare. Identifikasi lebih lanjut perlu dilakukan untuk mengetahui jenis fungi yang ada pada Sarcophyton sp.

Metabolites Profiling of Penicillium citrinum Recovered from Endophytic of Ramie (Boehmeria nivea) as a Potential Biocontrol Against Pathogenic Fungi Kartika Sari; Vira Kusuma Dewi; Asri Peni Wulandari; Nia Rossiana; Tati Herlina; Fitri Widiantini
HAYATI Journal of Biosciences Vol. 30 No. 2 (2023): March 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.2.246-255

Endophytes are a potent source of bioactive compounds that mimic plant-based metabolites. Fungi Fusarium spp. and Clonostachys rosea have been identified as pathogenic microorganisms in ramie (Boehmeria nivea). The antifungal test was carried out using the diffusion method and the MIC50 and Minimum Fungicidal Concentration (MFC) values using the tested microbial pathogens were Fusarium solani isolate 3248941, F. solani isolate Colpat-359, F. oxysporum isolate N-61-2, dan Clonostachys rosea strain B3042. Identification of secondary metabolites of the extract was carried out using GC-MS. The chromatogram of GC-MS analysis of this ethyl acetate extract (EA) showed seven dominant chemical compounds with various biological activities. Ethyl asetate extract of P. citrinum showed inhibition zone ranged from 19.10–22.07 mm with strong-very strong category at 200 mg ml-1 concentration against the tested microbial pathogens. Results revealed that the significant MIC values were observed against F. solani isolate 3248941 and Clonostachys rosea strain B3042 by less than 6.3  mg ml-1 and against F. solani isolate Colpat-359 and F. oxysporum isolate N-61-2 with 12.5 mg ml-1 and 25 mg ml-1 respectively. Minimum Fungicidal concentrations (MFC) for EA of P. citrinum were also reported against F. solani isolate 3248941 and C. rosea strain B3042, by 12.5 mg ml-1, whilst Fusarium solani isolate Colpat-359 by 25 mg ml-1 and Fusarium oxysporum isolate N-61-2 ,by 50 mg ml-1 respectively. Results of this study showed that P. citrinum, endophytic fungi of ramie, could be a promising source of compounds for antifungal agents.

Effect of Electron-Beam Irradiation on the Growth Profile and Fatty Acid Composition of Botryococcus sp. Windra Yuniarsih; Heli Siti Halimatul; Asri Peni Wulandari
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14891

Botryococcus sp. is an economically important microalgae as it contains carbohydrates, lipids, proteins, and a number of phytochemicals. The high concentrations of saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) in this strain also a valuable source for production of biofuel. Mutagenic engineering using irradiation has been known to increase the content of these biomass. The aim of this study is to know the effect of electron-beam irradiation to the growth profile and fatty acid composition of Botryococcus sp. In this study, Botryococcus sp. adjusted an optical density 1.0 after being exposed to different doses of electron-beam irradiation (160 kGy, 240 kGy, and 320 kGy) and induced random mutagenesis for strain improvement was observed based on high energy (1.5 MeV and 2 mA). Several mutants obtained were designated as strain B160, B240, and B320, respectively. The profile growth was significantly different between control (0 kGy) and irradiated microalgae strain. The highest growth is found in B320 mutant. Fatty acid of Botryococcus sp. control produced 7 fatty acids, B160 produced 7 fatty acids, B240 produced 6 fatty acids, whereas B320 produced 9 fatty acids. B320 produced hydrocarbon and phtalic acid as well as fatty acids. Total SFAs and MUFAs of B240 was increased 1.6 times compared to the wild type. The results would give some implications to improve the quality of biodiesel from Botryococcus sp.

Arohynapene A Produced by Penicillium steckii JB-NW-2-1 Isolated from Avicennia marina (Forssk.) Vierh and Its Cytotoxic Activities Yeni Mulyani; Wahyu Syafriansyah; Asri Peni Wulandari; Azmi Azhari; Sari Purbaya; Aprilia Permata Sari; Galih Bayu Pratama; Fajar Fauzi Abdullah; Kindi Farabi; Unang Supratman; Yoshihito Shiono
Indonesian Journal of Chemistry Vol 24, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.83982

Mangrove-associated endophytic fungi are producers of secondary metabolites in unique and diverse structures with interesting biological activities such as antiviral, antifungal, antibacterial, anti-inflammatory, and cytotoxic agents. Endophytes play an important role in the physiological activities of the host plants, influencing the improvement of resistance to stress, insects, nematodes, and diseases. In this study, arohynapene A, a polyketide compound, was successfully isolated from the mangrove-derived fungus Penicillium steckii JB-NW-2-1 obtained from mangrove plant Avicennia marina (Forssk) Vierh. The structure was determined by a spectroscopic method including IR, MS, 1D-, and 2D-NMR techniques. This compound was evaluated for cytotoxic activities using resazurin assay against four cancer cells, HeLa cervical, MCF-7 breast cancer, B16-F10 melanoma, and A549 lung adenocarcinoma. The results showed no significant activities against all cancer cells tested (IC50 > 500 µM).

Diketopiperazine Cyclo-(S-Pro-R-Leu) Produced by Periconia pseudobyssoides K5 Isolated from Toona sureni (Meliaceae) and its Heme Polymerization Inhibition Activity Azhari, Azmi; Harneti, Desi; Wulandari, Asri Peni; Mulyani, Yeni; Purbaya, Sari; Sari, Aprilia Permata; Pratama, Galih Bayu; Supratman, Unang; Shiono, Yoshihito
Makara Journal of Science Vol. 27, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Fungi endophytes are living microorganisms colonizing inside the internal tissue of a plant and provide ecological benefits for their host. Endophytes provide various metabolites for plant adaptation toward biotic and abiotic stresses and have tremendous pharmacological activities. Toona sureni (T. sureni) (Meliaceae) belongs to the Toona genera and is reported to have antimalarial activity. To determine the compounds produced by the endophytic fungus from this plant, we isolated a compound from Periconia pseudobyssoides (P. pseudobyssoides) K5 endophytes from the stem bark of T. sureni. Diketopiperazine cyclo-(S-Pro-R-Leu), a non-ribosomal peptide, was isolated from brown rice fermented at 28 ± 2 ℃ for 30 days. The structure was determined by spectroscopic methods including fourier-transform infrared spectroscopy, mass spectrometry, and 1D and 2D nuclear magnetic resonance techniques. This compound was evaluated for heme polymerization inhibition activity (HPIA) with an IC50 value of 9.89 ± 0.24 mmol/L compared with positive control chloroquine phosphate with an IC50 value of 3.08 ± 0.58 mmol/L. This compound has been categorized as having low activity three times lower than positive control chloroquine phosphate. This information provides new leads about the compound diketopiperazine cyclo-(S-Pro-R-Leu) produced by P. pseudobyssoides K5 endophytes having low activity in inhibiting heme polymerization. In the future, to explore the potency of this compound as antimalarial agent, the other antimalarial test such as lactate dehydrogenase assay might be useful.

Screening Mycelium of Macrofungi Isolates in Tahura Djuanda Bandung As A New Candidate for Biomaterial Inneke Andita Putri Laoli; Asri Peni Wulandari; Ulyaa Rachmawati; Dwi Ramadhani Sukmana; Annisa Handayani Dewi; Nia Rossiana
Jurnal Biodjati Vol 9, No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30021

Abstract. Cardamom (Amomum compactum) is an aromatic spice plant with numerous benefits, widely used in cooking, medicine, and beverages. The high demand for cardamom remains unmet due to the lengthy germination period required for cardamom seeds and the inability of cardamom shoots to thrive when planted independently from the mother plant. The presence of the mother plant significantly impacts cardamom nurseries utilizing shoots, making it challenging to obtain large quantities of nursery transplants. Growth stimulants, such as vitamin B1 and Indole Butyric Acid (IBA), are required to enhance vegetative growth in plants. Vitamin B1 (IPI brand) is applied due to its ease of accessibility and cost-effectiveness. Meanwhile, IBA is utilized for its accessibility, stable chemical content, and prolonged efficacy. This research aims to determine the optimal concentration of vitamin B1, IBA, and their combination to enhance the growth of mother and tiller shoots of cardamom ex vitro. The research employed a two-factorial Randomized Complete Block Design, with vitamin B1 concentrations of 21.5% and 43% and IBA concentrations of 0.75 ppm and 150 ppm. The treatment V1I1 (vitamin B1 21.5% and IBA 75 ppm) on mother plant shoots maintained a survival rate of 67% up to 12 Weeks After Planting (WAP). The interaction between vitamins B1 and IBA exhibited no significant effect on all parameters of mother shoots and tillers; however, vitamin B1 significantly influenced the vegetative growth of cardamom mother shoots. Vitamin B1 at 43% produced a significantly higher number of leaves compared to 21.5%. Thus, vitamin B1 at 43% is recommended for cardamom propagation, while vitamin B1 at 21.5% has the potential to enhance the average growth of tiller shoots across all parameters. HTML Source code: Abstract. Cardamom (Amomum compactum) is an aromatic spice plant with numerous benefits, widely used in cooking, medicine, and beverages. The high demand for cardamom remains unmet due to the lengthy germination period required for cardamom seeds and the inability of cardamom shoots to thrive when planted independently from the mother plant. The presence of the mother plant significantly impacts cardamom nurseries utilizing shoots, making it challenging to obtain large quantities of nursery transplants. Growth stimulants, such as vitamin B1 and Indole Butyric Acid (IBA), are required to enhance vegetative growth in plants. Vitamin B1 (IPI brand) is applied due to its ease of accessibility and cost-effectiveness. Meanwhile, IBA is utilized for its accessibility, stable chemical content, and prolonged efficacy. This research aims to determine the optimal concentration of vitamin B1, IBA, and their combination to enhance the growth of mother and tiller shoots of cardamom ex vitro. The research employed a two-factorial Randomized Complete Block Design, with vitamin B1 concentrations of 21.5% and 43% and IBA concentrations of 0.75 ppm and 150 ppm. The treatment V1I1 (vitamin B1 21.5% and IBA 75 ppm) on mother plant shoots maintained a survival rate of 67% up to 12 Weeks After Planting (WAP). The interaction between vitamins B1 and IBA exhibited no significant effect on all parameters of mother shoots and tillers; however, vitamin B1 significantly influenced the vegetative growth of cardamom mother shoots. Vitamin B1 at 43% produced a significantly higher number of leaves compared to 21.5%. Thus, vitamin B1 at 43% is recommended for cardamom propagation, while vitamin B1 at 21.5% has the potential to enhance the average growth of tiller shoots across all parameters. Chat GPT: Abstract. Cardamom (Amomum compactum) is an aromatic spice plant with numerous benefits, widely used in cooking, medicine, and beverages. The high demand for cardamom remains unmet due to the lengthy germination period required for cardamom seeds and the inability of cardamom shoots to thrive when planted independently from the mother plant. The presence of the mother plant significantly impacts cardamom nurseries utilizing shoots, making it challenging to obtain large quantities of nursery transplants. Growth stimulants, such as vitamin B1 and Indole Butyric Acid (IBA), are required to enhance vegetative growth in plants. Vitamin B1 (IPI brand) is applied due to its ease of accessibility and cost-effectiveness. Meanwhile, IBA is utilized for its accessibility, stable chemical content, and prolonged efficacy. This research aims to determine the optimal concentration of vitamin B1, IBA, and their combination to enhance the growth of mother and tiller shoots of cardamom ex vitro. The research employed a two-factorial Randomized Complete Block Design, with vitamin B1 concentrations of 21.5% and 43% and IBA concentrations of 0.75 ppm and 150 ppm. The treatment V1I1 (vitamin B1 21.5% and IBA 75 ppm) on mother plant shoots maintained a survival rate of 67% up to 12 Weeks After Planting (WAP). The interaction between vitamins B1 and IBA exhibited no significant effect on all parameters of mother shoots and tillers; however, vitamin B1 significantly influenced the vegetative growth of cardamom mother shoots. Vitamin B1 at 43% produced a significantly higher number of leaves compared to 21.5%. Thus, vitamin B1 at 43% is recommended for cardamom propagation, while vitamin B1 at 21.5% has the potential to enhance the average growth of tiller shoots across all parameters. Abstract. Cardamom (Amomum compactum) is an aromatic spice plant with numerous benefits, widely used in cooking, medicine, and beverages. The high demand for cardamom remains unmet due to the lengthy germination period required for cardamom seeds and the inability of cardamom shoots to thrive when planted independently from the mother plant. The presence of the mother plant significantly impacts cardamom nurseries utilizing shoots, making it challenging to obtain large quantities of nursery transplants. Growth stimulants, such as vitamin B1 and Indole Butyric Acid (IBA), are required to enhance vegetative growth in plants. Vitamin B1 (IPI brand) is applied due to its ease of accessibility and cost-effectiveness. Meanwhile, IBA is utilized for its accessibility, stable chemical content, and prolonged efficacy. This research aims to determine the optimal concentration of vitamin B1, IBA, and their combination to enhance the growth of mother and tiller shoots of cardamom ex vitro. The research employed a two-factorial Randomized Complete Block Design, with vitamin B1 concentrations of 21.5% and 43% and IBA concentrations of 0.75 ppm and 150 ppm. The treatment V1I1 (vitamin B1 21.5% and IBA 75 ppm) on mother plant shoots maintained a survival rate of 67% up to 12 Weeks After Planting (WAP). The interaction between vitamins B1 and IBA exhibited no significant effect on all parameters of mother shoots and tillers; however, vitamin B1 significantly influenced the vegetative growth of cardamom mother shoots. Vitamin B1 at 43% produced a significantly higher number of leaves compared to 21.5%. Thus, vitamin B1 at 43% is recommended for cardamom propagation, while vitamin B1 at 21.5% has the potential to enhance the average growth of tiller shoots across all parameters. <!DOCTYPE html><html lang="en"><head><meta charset="UTF-8"><meta name="viewport" content="width=device-width, initial-scale=1.0"><title>Abstract</title><style> p { text-align: justify; }</style></head><body> Abstract. Cardamom (Amomum compactum) is an aromatic spice plant with numerous benefits, widely used in cooking, medicine, and beverages. The high demand for cardamom remains unmet due to the lengthy germination period required for cardamom seeds and the inability of cardamom shoots to thrive when planted independently from the mother plant. The presence of the mother plant significantly impacts cardamom nurseries utilizing shoots, making it challenging to obtain large quantities of nursery transplants. Growth stimulants, such as vitamin B1 and Indole Butyric Acid (IBA), are required to enhance vegetative growth in plants. Vitamin B1 (IPI brand) is applied due to its ease of accessibility and cost-effectiveness. Meanwhile, IBA is utilized for its accessibility, stable chemical content, and prolonged efficacy. This research aims to determine the optimal concentration of vitamin B1, IBA, and their combination to enhance the growth of mother and tiller shoots of cardamom ex vitro. The research employed a two-factorial Randomized Complete Block Design, with vitamin B1 concentrations of 21.5% and 43% and IBA concentrations of 0.75 ppm and 150 ppm. The treatment V1I1 (vitamin B1 21.5% and IBA 75 ppm) on mother plant shoots maintained a survival rate of 67% up to 12 Weeks After Planting (WAP). The interaction between vitamins B1 and IBA exhibited no significant effect on all parameters of mother shoots and tillers; however, vitamin B1 significantly influenced the vegetative growth of cardamom mother shoots. Vitamin B1 at 43% produced a significantly higher number of leaves compared to 21.5%. Thus, vitamin B1 at 43% is recommended for cardamom propagation, while vitamin B1 at 21.5% has the potential to enhance the average growth of tiller shoots across all parameters. </body></html> Abstract. Cardamom (Amomum compactum) is an aromatic spice plant with numerous benefits, widely used in cooking, medicine, and beverages. The high demand for cardamom remains unmet due to the lengthy germination period required for cardamom seeds and the inability of cardamom shoots to thrive when planted independently from the mother plant. The presence of the mother plant significantly impacts cardamom nurseries utilizing shoots, making it challenging to obtain large quantities of nursery transplants. Growth stimulants, such as vitamin B1 and Indole Butyric Acid (IBA), are required to enhance vegetative growth in plants. Vitamin B1 (IPI brand) is applied due to its ease of accessibility and cost-effectiveness. Meanwhile, IBA is utilized for its accessibility, stable chemical content, and prolonged efficacy. This research aims to determine the optimal concentration of vitamin B1, IBA, and their combination to enhance the growth of mother and tiller shoots of cardamom ex vitro. The research employed a two-factorial Randomized Complete Block Design, with vitamin B1 concentrations of 21.5% and 43% and IBA concentrations of 0.75 ppm and 150 ppm. The treatment V1I1 (vitamin B1 21.5% and IBA 75 ppm) on mother plant shoots maintained a survival rate of 67% up to 12 Weeks After Planting (WAP). The interaction between vitamins B1 and IBA exhibited no significant effect on all parameters of mother shoots and tillers; however, vitamin B1 significantly influenced the vegetative growth of cardamom mother shoots. Vitamin B1 at 43% produced a significantly higher number of leaves compared to 21.5%. Thus, vitamin B1 at 43% is recommended for cardamom propagation, while vitamin B1 at 21.5% has the potential to enhance the average growth of tiller shoots across all parameters. Abstract. Type 2 diabetes mellitus (DM) is a chronic metabolic disorder characterized by elevated blood glucose levels due to insulin resistance. Type 2 diabetes is considerably more prevalent than other forms (85–90%). The risk of type 2 diabetes is higher in women (53.2%). There is an urgent requirement for better and more affordable treatment options considering DM therapy is expensive and may have adverse health effects. The study's objective is to examine how acute toxicity, blood glucose levels, and body weight are affected by Moringa leaf ethanol extract (MLEE). Maceration was employed to eliminate the leaves of the moringa plant. Phytochemical screening was completed to assess the total flavonoid content and screen for alkaloids, flavonoids, phenolics, saponins, and tannins. Acute toxicity testing was performed following OECD guideline 423. Clinical symptoms of acute toxicity were observed every 30 minutes for the first 24 hours post-treatment, followed by observations every 24 hours up to 14 days. The estimated LD50 range was determined. Streptozotocin/ nicotinamide-induced female Mus musculus was administered to evaluate the antidiabetic potential of MLEE. Six groups of mice were utilized, which included a healthy control group (aquades not induced), a negative control group (induced aquades), a positive control group (induced glimepiride 0.8 mg/kg BW), and three treatment groups with varying dosages of MLEE (induced; 0, 100, and 150 mg/kg BW). A semi-auto chemical analyzer was employed on days 0 through 31 to determine blood glucose levels. An analytical digital balance was utilized to calculate the body weight. With a total flavonoid concentration of 20.75%, MLEE incorporated alkaloids, flavonoids, phenolics, saponins, and tannins. MLEE demonstrated a significant effect in lowering blood glucose levels at a dose of 100 mg/kg BW (P<0.05). A significant positive correlation has been identified between body weight and blood glucose levels (P<0.05). Soil arthropods play an important role in nutrient cycling and maintenance of soil structure. Thus, their abundance and diversity can indicate the biological quality of the soil. Due to different soil management practices, arthropods are also very sensitive to environmental changes. This study aims to analyze the abundance and diversity of soil arthropods and the environmental factors that support the abundance of soil arthropods in terrestrial of Situ Lengkong Panjalu, West Java, Indonesia. The methods used to obtain samples of soil arthropods are pitfall trap. Soil arthropod data were analyzed to determine the Shannon diversity index (H'), Margalef richness index (R), and Evenness index (E). The effect of abiotic environmental factors was analyzed using Principal Component Analysis (PCA). A total of 1263 arthropods were found in Situ Lengkong Panjalu, belonging to 11 orders, 24 families, and 32 morphospecies. The current work determined the scores of H'= 2.08, R = 3,08, and E = 0.72 of soil arthropods in Situ Lengkong Panjalu, West Java, Indonesia. Results revealed that humidity is one such environmental parameter affecting the presence of soil arthropods in Situ Lengkong Panjalu, West Java, Indonesia. This research can be used as a guide in validating and conservation of the habitat of soil arthropod species on West Java Obesity has a 15-fold higher risk of coronary heart disease, stroke, and diabetes mellitus. Microalga isone of the natural resources that potentially treat obesity. The purpose of this study was to evaluate the total phenolic contents (TPC), antioxidant, and anti-obesity properties of ethanolic extract of microalgae strain MRB-2. The TPC was determined using the Follin-Ciocalteu method. The antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, and the anti-obesity was analyzed using an anti-lipase pancreatic assay. The morphology of microalga cells was also determined using Scanning Electron Microscopy (SEM). The results revealed that the TPC of ethanolic extract from the ultrasound extraction method was higher than the maceration method with the value of 2.75±0.26 mg GAE/g. While the scavenging activity toward DPPH radicals of ethanolic extract from the maceration method was higher than ultrasound, with a value of 38.92±1.94% at 0.8 mg/mL. The lipase inhibitory activity of extract from the maceration method was higher than ultrasound with a value of 20.81±2.24% at 0.38 mg/mL. Our results indicate that ethanolic extract of MRB-2 was potentially developed for anti-obesity foods and health-functional foods derived from new peatland microalgae. Talinum paniculatum Jacq (Gaertn), locally known as Javanese Ginseng, is an essential medicinal plant characterized by high flavonoid content with antioxidant and antimicrobial activities. Compared to other natural sources, a larger number of T. paniculatum leaves is required to obtain high amounts of flavonoid for drug development. In this context, in vitro culture can be used to increase flavonoid production in a controlled condition without reducing plant population. The concentration of Plant Growth Regulators (PGRs) is capable of influencing biomass and flavonoid accumulation in callus culture from several species. Despite these benefits, there is limited information about the optimal concentration of PGRs for flavonoid production from T. paniculatum callus culture. Therefore, this study aimed to investigate the optimum concentration of 2,4-D and kinetin for callus growth, as well as flavonoid production from T. paniculatum. The investigation was carried out using a completely randomized design (CRD), where young leaves explants were cultured on MS media supplemented with various concentrations of 2,4-D (0, 0.5, 1, 2) mg/L and kinetin (0, 0.5, 1, 2) mg/L for callus and flavonoid production. The results showed that the highest callus biomass of 0.105 g was produced from MS medium with 2 mg/L of 2,4-D and 1 mg/L of kinetin. Meanwhile, the highest total flavonoid content of 25.66 mg QE/g DW was produced from media supplemented with 1 mg/Lof 2,4-D and 2 mg/L of kinetin. These results showed that different combinations of 2,4-D and kinetin were required to increase biomass and flavonoid production. In conclusion, this study provided valuable information for the development of bioactive compounds through in vitro culture. The mistletoe (Scurrula ferruginea) is a parasitic plant obtaining nutrients and water from host plants at Nglinggo tea plantation, Kulon Progo, Yogyakarta. Despite the numerous benefits of Scurrula ferruginea leaf, substantiation regarding its pharmacological activity is still lacking. Therefore, this research aimed to identify Scurrula ferruginea species and obtain secondary metabolites with antibacterial activity. The experiment was carried out by extracting Scurrula ferruginea leaf using maceration methods with 96% ethanol, followed by qualitatively and quantitatively phytochemical screening, inhibitory zone test, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) test. Several compounds, such as flavonoids, tannins, phenols, saponins, alkaloids, terpenoids, and steroids, were identified in the mistletoe extract. The results showed total flavonoid, phenolic, and tannin levels were 7.41 ± 0.38 mg QE/mg, 1672 ± 50.99 mg GAE/g, and 1.43 ± 0.15 mg TAE/g, respectively. Scurrula ferruginea leaf ethanolic extract showed the potential to form an inhibition zone against S. aureus in the moderate category at 525 mg/mL, but no significant activity was observed against E. coli. The MIC results of Scurrula ferruginea leaf ethanolic extract against S. aureus were 0.6%, and the MBC was at 2.4%. Furthermore, the MIC value against E. coli was 14%, and the MBC value was 28%. Based on these results, it could be concluded that Scurrula ferruginea leaf ethanolic extract showed high effectiveness in inhibiting and killing S. aureus bacteria compared to E. coli, with MIC and MBC values of 0.6% and 2.4%, respectively Grand Forest Park (Tahura) Ir. H. Djuanda, Bandung, West Java, Indonesia is a secondary nature conservation area rich in biodiversity, including macrofungi which has not been widely studied. The research aims to isolate, characterize its morphology and screen its potential as a biomaterial source based on the growth of macrofungal mycelium. The research was carried out using an exploration method at three sampling locations: Maribaya (trail area), Goa Jepang (cave area), and Curug Koleang (waterfall area). The obtained macrofungi were isolated and identified based on their macroscopic and microscopic morphological characteristics and coded based on the location and number of isolates. A comparative evaluation was carried out by one-way Analysis of variance (ANOVA) to assess the average mycelial growth of the macrofungal isolates for 9 days on PDA. The results showed that there was a total of 62 species of macrofungi from three locations: 22 isolates from the Maribaya (MB) area, 18 isolates from the Goa Jepang (GJP) area, and 22 isolates from the Curug Koleang (CK) area. The isolates that showed the highest mycelium length and represented each research area were shown by MB-07 (63.98 ± 1.21 mm), GJP-01 (81.47 ± 0.41 mm) and CK-13 (72.14 ± 1.20 mm). Isolate GJP-01 from the Goa Jepang area has the potential to become a superior fungus in its ability to expand mycelium should be developed for mycelium-based material applications. Stevia rebaudiana Bert. (Stevia) is used in the commercial and health industries because of its steviol glycosides (stevioside, rebaudioside A, rebaudioside C) and flavonoids. Polyploidy induction of stevia plants using oryzalin was held to increase the diversity and produce superior varieties. This research aims to obtain stevia diversity with different characteristics from its diploid. The Tawangmangu variety of stevia seedlings was experimentally designed using a completely random design. Nine combinations of oryzalin concentrations (1.5, 2.5, and 3.5μM) and immersion times (4, 6, and 8 hours) treatments were applied to 15 stevia seedlings each. Stevia without treatment was used as a control. Treatments were applied directly to the shoots on the second internode from the tip. Observations on target shoots included the number of survivors, morphological variables (height, number of internodes, internode length, leaf size, leaf thickness, stem diameter, and leaf color), stomata, and plant ploidy level. Data analysis on the number of live plants used frequency and descriptively. Morphological data consisting of plant height, number of shoots, number of internodes, internode length, leaf length, leaf width, leaf thickness, and stem diameter were analyzed using boxplot graphs and descriptive to describe the diversity of M1 stevia treatment results. Leaf color and stomata were analyzed descriptively. The results showed that up to 3.5μM concentrations of oryzalin and 6 hours of immersion time were safe to use as a mutation agent with above 67% survival rate. Various oryzalin treatments of Tawangmangu stevia varieties yielded polyploidy morphological growth indications in height, number of internodes, internode length, stem diameter, leaf size, leaf thickness, leaf color, stomata, and stem diameter. In addition, there are growth variations such as chimeras, rosettes, and leaf splitting. However, further flow cytometry tests showed that oryzalin concentration and immersion duration directly on the vegetative material did not produce polyploid stevia individuals.

DEGRADASI BIOMASSA KULIT BATANG RAMI (Boehmeria Nivea) BERDASARKAN AKTIVITIVITAS LIGNOSELULOXILANOLITIK DARI Aspergillus, Penicillium, DAN Trycophyton Fatih Awliya, Meuthia; Dewi, Maranti; Syifa Nabila, Devina; Peni Wulandari, Asri
BIOTIKA Jurnal Ilmiah Biologi Vol 21, No 1 (2023): BIOTIKA JUNI 2023
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v21i1.36223

Biomassa yang berasal dari tanaman pada umumnya mengandung selulosa, lignin, dan xilan yang sebagian besar sebagai buangan sisa dari kegiatan agroindustri. Hasil pengomposan biomassa buangan rami diperoleh tiga isolat mikrofungi yang dikode sebagai FKR-A1, FKR-D5, dan FKR-D17. Tujuan Penelitian ini adalah mengetahui potensi isolat mikrofungi berdasarkan aktivitas enzim ligninase, selulase, dan xylanase (lignoseluloxilanotik) dalam mendegradasi biomassa rami. Proses degradasi selulosa serat dan serat kasar rami, serta kulit batang rami sisa dekortikasi menggunakan metode fermentasi terendam (SMF) dengan waktu pengamatan selama 10 hari. Pengamatan secara kualitatif dilakukan untuk mengetahui perubahan karakter morfologi biomassa rami. Pengujian aktivitas enzim ditentukan secara kuantitatif dengan metode asam 3,5-dinitrosalisilat menggunakan spektrofotometri UV-Vis. Standar yang digunakan adalah glukosa dengan reagen dinitrosalisilat serta substrat masing -terdiri dari Carboxymethyl Cellulose (CMC), xilan, dan lignin. Hasil penelitian ini menunjukkan ketiga isolat fungi yang diidentifikasi sebagai Aspegillus, Penicillium, dan mempunyai aktivitas enzim ligninase, selulase, dan xilanase. Aspergilus, menunjukkan aktivitas ligninase, selulase, dan xylanase tertinggi dengan masing-masing sebesar 2,932 U/ml, 2,072 U/ml, dan 1,909 U/ml. Isolat terseleksi tersebut berpotensi untuk dikembangkan sebagai biostarter dalam proses degradasi biomassa tanaman lainnya.

Steroids Produced by Endophytic Fungus Lasiodiplodia Theobromae From Aglaia Argentea Blume and Their Cytotoxic Activity Against Hela Cervical Cancer Cell Lines Supratman, Unang; Purbaya, Sari; Harneti, Desi; Wulandari, Asri Peni; Mulyani, Yeni; Azhari, Azmi; Sari, Aprilia Permata
Molekul Vol 18 No 3 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.3.7824

Three steroids, ergosterol (1), ergosterol peroxide (2) and stigmasterol (3) have been isolated from endophytic fungus, Lasiodiplodia theobromae derived from the root of Aglaia argentea Blume. The steroids were isolated by vacuum chromatography and column chromatography, the chemical structure was established following the analysis of 1D-NMR, 2D-NMR, IR, MS and by comparison with previously reported spectra data. Ergosterol peroxide (2) and stigmasterol (3) were reported for the first time isolated from Lasiodiplodia theobromae endophytic fungus. Cytotoxic activities of the compounds were tested with resazurin assay against HeLa cervical cancer cells, compound 2 displayed strongest cytotoxic activities against HeLa cervical cancer cells with IC50 values of 0.28 µM, while compounds 1 and 3 showed IC50 values of 0.34 µM and 27.32 µM, respectively

ISOLASI, IDENTIFIKASI, DAN JUMLAH BAKTERI ENDOFIT ASAL JAMUR TIRAM COKELAT DAN SHIITAKE BERDASARKAN KANDUNGAN ASAM GLUTAMAT Oktaviani, Anti; Indrawati, Ida; Marzuki, Betty Mayawatie; Wulandari, Asri Peni
BIOTIKA Jurnal Ilmiah Biologi Vol 22, No 1 (2024): BIOTIKA JUNI 2024
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v22i1.39515

Jamur tiram cokelat dan shiitake merupakan jamur pangan yang mengandung berbagai vitamin, mineral, serta mengandung asam glutamat alami. Studi mengenai pengaruh asam glutamat terhadap keberadaan bakteri endofit yang berasal dari jamur tiram cokelat dan shiitake belum pernah dilakukan. Penelitian ini bertujuan untuk memperoleh isolat bakteri endofit dan mengidentifikasi makroskopik dan mikroskopik, serta mengkaji kandungan asam glutamatnya. Perhitungan jumlah bakteri dilakukan dengan metode TPC, lalu diidentifikasi secara makroskopik dan mikroskopik, selanjutnya kandungan asam glutamat pada jamur tiram cokelat dan shiitake dianalisis menggunakan HPLC. Hasil isolasi bakteri menghasilkan enam isolat dengan kode IJTC dan IJS. Pengamatan mikroskopik menunjukkan isolat bakteri terdiri dari gram positif dan negatif dengan morfologi kokus dan basil. Total Plate Count dari isolat jamur tiram coklat dan jamur shiitake berturut-turut adalah 10,18 x 103 CFU/g dan 7,2 x 103 CFU/g. Analisis kandungan asam amino menunjukkan total asam glutamat tertinggi diperoleh pada jamur shiitake sebesar 58.297,31 mg/kg, sementara jamur tiram cokelat sebanyak 33.850,32 mg/kg. Berdasarkan total kandungan asam glutamat tidak mempengaruhi jumlah bakteri endofit. Selain itu, kandungan metabolit sekunder bakteri endofit jamur tiram cokelat dan shiitake belum diketahui aktivitas biologisnya sehingga memerlukan penelitian lebih lanjut untuk memberikan potensi terhadap bioprospeksi edible mushroom.

Co-Authors Abdul Rohmat Ade Zuhrotun Afina Yusradinan Annas Nursyahid Anne Nuraini Annisa Abdiwijaya Qaromah Annisa Handayani Dewi Aprilia Permata Sari Ayu Wandira Azmi Azhari Azmi Azhari, Azmi Baghya Nugraha Betty Mayawatie Marzuki, Betty Mayawatie Danni Ramdani Desi Harneti Putri Huspa Dewi, Annisa Handayani Dewi, Maranti Dwi Margiati Dwi Ramadhani Sukmana Elisabeth, Pauline Ema Wulandini Eva Aprilia Fadillah, Muhammad Nugraha Faizatul Falah Faizatul FALAH Fajar Fauzi Abdullah Fatih Awliya, Meuthia Felicia Fiandisty Fitri Widiantini Galih Bayu Pratama Heli Siti Halimatul Hutajulu, Esther Christy Natasya Ida Indrawati Ida Indrawati Ida Indrawati Inneke Andita Putri Laoli Irfan Zidni Ismayati, Maya Karen Kezia Lolowang Kartika Sari Kindi Farabi Kusumah, Sukma Surya Laksana, Raden Permana Budi Laoli, Inneke Andita Putri Lubis, Muhammad Adly Rahandi Manggar Arum Aristri Maya Ismayati Michelle Azista N. Casandra Mohamad Nurzaman Muhammad Adly Rahandi Lubis Muhammad Rasyidur Ridho Muhammadiyah, Muhammadiyah Murgayanti Murgayanti Nia Rossiana Nidia Primastia Ningsih, Nur Hasna Nurindah Nurindah Nurindah Oktaviani, Anti Poniah Andayaningsih Pratama, Galih Bayu Purbaya, Sari Rachmawati, Ulyaa Raden Permana Budi Laksana Rahayuningsih, Sri Redjeki Rusky Intan Pratama Sari Purbaya Sari, Aprilia Permata Shiono, Yoshihito Sukmana, Dwi Ramadhani Syifa Nabila, Devina Tati Herlina Ulyaa Rachmawati Unang Supratman Victor Handoko Vira Kusuma Dewi Wahyu Syafriansyah Widya Fatriasari Widya FATRIASARI Widya, Shafira Nurul Windra Yuniarsih Yeni Mulyani Yoshihito Shiono Yuli Andriani Yuniarsih, Windra

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