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All Journal HAYATI Journal of Biosciences Biotika: Jurnal Ilmiah Biologi Indonesian Journal of Pharmaceutical Science and Technology Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Kultivasi Jurnal Biodjati Chimica et Natura Acta Indonesian Journal of Chemistry Jurnal Mikologi Indonesia Biosaintifika: Journal of Biology & Biology Education JURNAL SELULOSA 3BIO: Journal of Biological Science, Technology and Management Proceeding Biology Education Conference Indonesian Journal of Medical Sciences and Public Health Jurnal Sylva Lestari Makara Journal of Science Journal of Fibers and Polymer Composites Molekul: Jurnal Ilmiah Kimia

Asri Peni Wulandari

Laboratorium Mikrobiologi, Departemen Biologi, FMIPA, Universitas Padjadjaran

Author-ID : 557931

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Veterinary

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UJI AKTIVITAS ANTIBAKTERI BAKTERI ENDOFIT JAMUR LINGZHI (Ganoderma lucidum) DAN MAITAKE (Grifola frondosa) TERHADAP BAKTERI PATOGEN PLAK GIGI Widya, Shafira Nurul; Indrawati, Ida; Wulandari, Asri Peni
BIOTIKA Jurnal Ilmiah Biologi Vol 21, No 2 (2023): BIOTIKA DESEMBER 2023
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v21i2.36285

Jamur maitake (Grifola frondosa) dan jamur lingzhi (Ganoderma lucidum) dilaporkan memiliki berbagai macam metabolit sekunder yang dapat dimanfaatkan sebagai agen pengobatan penyakit pada manusia. Studi mengenai aktivitas antibakteri jamur maitake dan lingzhi terhadap bakteri penyebab plak gigi belum pernah dilakukan sebelumnya. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri bakteri endofit yang diisolasi dari jamur maitake dan jamur lingzhi terhadap bakteri patogen plak gigi. Metode yang digunakan pada penelitian ini yaitu meliputi isolasi, identifikasi makroskopik dan mikroskopik, serta uji aktivitas antibakteri dengan metode Kirby-Bauer. Hasil Isolasi bakteri dari jamur maitake (M) dan lingzhi (L) didapatkan empat isolat, masing-masing: isolat M1, M2, L2 dan L3 yang merupakan bakteri Bacillus sp. Zona hambat yang dihasilkan pada uji aktivitas antibakteri dihasilkan zona hambat sebesar 11 mm pada isolat M1 dan M2, 13 mm pada isolat L2 dan 12 mm pada isolat L3. Isolat L2 berpotensi sebagai kandidat isolat unggulan yang dapat dikembangkan untuk aplikasi kesehatan khususnya karies gigi dan infeksi pada rongga mulut.

Evaluation of Ramie Bark and Albasia Sawdust Substrates for Mycelium-Based Composites Using Leiotrametes lactinea Wulandari, Asri Peni; Sukmana, Dwi Ramadhani; Kusumah, Sukma Surya; Fadillah, Muhammad Nugraha; Abdul Rohmat
Journal of Fibers and Polymer Composites Vol. 3 No. 2 (2024): Journal of Fibers and Polymer Composites
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jfpc.v3i2.207

Mycelium-based composite (MBC) has significant potential to utilize agricultural biomass waste. The use of fungi that are commonly used as MBC materials is still very limited to certain types. This study aims to test the MBC characteristics of the growth of mycelium Leiotrametes lactinea MYCL-3 on three different media substrates containing 72% Albasia (SA) sawdust, 72% ramie bark (BR), and 30% Albasia + 47% ramie mixture (SA-RB). The resulting MBC has dimensions of 20 x 5 x 5 cm and is yellowish-white in color. The structure of MBC shows that the density is higher (BR 0.28%). The absorption percentages for SA, RB, and SA-RB were 28.66, 21.37, and 27.22, respectively. The biodegradability of MBC showed that by day 10, the sample had degraded by about 14.14–17.46%. Mechanical testing for compression strength determined values for SA (117 kPa), RB (350 kPa), and SA-RB (140 kPa), with a final strength at 464 kPa, and the effect of the mixed media reduced the tensile strength of the composite to 277 kPa. The IR spectral results showed that the three composite samples did not show much different structures, but SA composites were known to have more types of constituent compounds not found in BR and SA-RB composites such as C=C (alkenes) bending, O-H (alcohol) associations, C-O-C (glycosidic) stretching, O-H (acid) bending, and C=C (benzene) stretching. Future investigations may focus on improving aggregate interlocking to increase strength and flexibility, tailoring MBC for specific applications.

Screening Mycelium of Macrofungi Isolates in Tahura Djuanda Bandung As A New Candidate for Biomaterial Laoli, Inneke Andita Putri; Wulandari, Asri Peni; Rachmawati, Ulyaa; Sukmana, Dwi Ramadhani; Dewi, Annisa Handayani; Rossiana, Nia
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30021

Â Â Abstract. Cardamom (Amomum compactum) is an aromatic spice plant with numerous benefits, widely used in cooking, medicine, and beverages. The high demand for cardamom remains unmet due to the lengthy germination period required for cardamom seeds and the inability of cardamom shoots to thrive when planted independently from the mother plant. The presence of the mother plant significantly impacts cardamom nurseries utilizing shoots, making it challenging to obtain large quantities of nursery transplants. Growth stimulants, such as vitamin B1 and Indole Butyric Acid (IBA), are required to enÂhance vegetative growth in plants. Vitamin B1 (IPI brand) is applied due to its ease of accessibility and cost-effectiveness. Meanwhile, IBA is utilized for its accessibility, stable chemical content, and prolonged efficacy. This research aims to determine the optimal concentration of vitamin B1, IBA, and their combination to enhance the growth of mother and tiller shoots of cardamom ex vitro. The research employed a two-factorial Randomized Complete Block Design, with vitamin B1 concentrations of 21.5% and 43% and IBA concentrations of 0.75 ppm and 150 ppm. The treatment V1I1 (vitamin B1 21.5% and IBA 75 ppm) on mother plant shoots maintained a survival rate of 67% up to 12 Weeks After Planting (WAP). The interaction between vitamins B1 and IBA exhibited no significant effect on all parameters of mother shoots and tillers; however, vitamin B1 significantly influenced the vegetative growth of cardamom mother shoots. Vitamin B1 at 43% produced a significantly higher number of leaves compared to 21.5%. Thus, vitamin B1 at 43% is recommended for cardamom propagation, while vitamin B1 at 21.5% has the potential to enhance the average growth of tiller shoots across all parameters.Â HTML Source code: Abstract. Cardamom (Amomum compactum) is an aromatic spice plant with numerous benefits, widely used in cooking, medicine, and beverages. The high demand for cardamom remains unmet due to the lengthy germination period required for cardamom seeds and the inability of cardamom shoots to thrive when planted independently from the mother plant. The presence of the mother plant significantly impacts cardamom nurseries utilizing shoots, making it challenging to obtain large quantities of nursery transplants. Growth stimulants, such as vitamin B1 and Indole Butyric Acid (IBA), are required to enÂhance vegetative growth in plants. Vitamin B1 (IPI brand) is applied due to its ease of accessibility and cost-effectiveness. Meanwhile, IBA is utilized for its accessibility, stable chemical content, and prolonged efficacy. This research aims to determine the optimal concentration of vitamin B1, IBA, and their combination to enhance the growth of mother and tiller shoots of cardamom ex vitro. The research employed a two-factorial Randomized Complete Block Design, with vitamin B1 concentrations of 21.5% and 43% and IBA concentrations of 0.75 ppm and 150 ppm. The treatment V1I1 (vitamin B1 21.5% and IBA 75 ppm) on mother plant shoots maintained a survival rate of 67% up to 12 Weeks After Planting (WAP). The interaction between vitamins B1 and IBA exhibited no significant effect on all parameters of mother shoots and tillers; however, vitamin B1 significantly influenced the vegetative growth of cardamom mother shoots. Vitamin B1 at 43% produced a significantly higher number of leaves compared to 21.5%. Thus, vitamin B1 at 43% is recommended for cardamom propagation, while vitamin B1 at 21.5% has the potential to enhance the average growth of tiller shoots across all parameters. Chat GPT: Abstract. Cardamom (Amomum compactum) is an aromatic spice plant with numerous benefits, widely used in cooking, medicine, and beverages. The high demand for cardamom remains unmet due to the lengthy germination period required for cardamom seeds and the inability of cardamom shoots to thrive when planted independently from the mother plant. The presence of the mother plant significantly impacts cardamom nurseries utilizing shoots, making it challenging to obtain large quantities of nursery transplants. Growth stimulants, such as vitamin B1 and Indole Butyric Acid (IBA), are required to enÂhance vegetative growth in plants. Vitamin B1 (IPI brand) is applied due to its ease of accessibility and cost-effectiveness. Meanwhile, IBA is utilized for its accessibility, stable chemical content, and prolonged efficacy. This research aims to determine the optimal concentration of vitamin B1, IBA, and their combination to enhance the growth of mother and tiller shoots of cardamom ex vitro. The research employed a two-factorial Randomized Complete Block Design, with vitamin B1 concentrations of 21.5% and 43% and IBA concentrations of 0.75 ppm and 150 ppm. The treatment V1I1 (vitamin B1 21.5% and IBA 75 ppm) on mother plant shoots maintained a survival rate of 67% up to 12 Weeks After Planting (WAP). The interaction between vitamins B1 and IBA exhibited no significant effect on all parameters of mother shoots and tillers; however, vitamin B1 significantly influenced the vegetative growth of cardamom mother shoots. Vitamin B1 at 43% produced a significantly higher number of leaves compared to 21.5%. Thus, vitamin B1 at 43% is recommended for cardamom propagation, while vitamin B1 at 21.5% has the potential to enhance the average growth of tiller shoots across all parameters.Â Â Abstract. Cardamom (Amomum compactum) is an aromatic spice plant with numerous benefits, widely used in cooking, medicine, and beverages. The high demand for cardamom remains unmet due to the lengthy germination period required for cardamom seeds and the inability of cardamom shoots to thrive when planted independently from the mother plant. The presence of the mother plant significantly impacts cardamom nurseries utilizing shoots, making it challenging to obtain large quantities of nursery transplants. Growth stimulants, such as vitamin B1 and Indole Butyric Acid (IBA), are required to enÂhance vegetative growth in plants. Vitamin B1 (IPI brand) is applied due to its ease of accessibility and cost-effectiveness. Meanwhile, IBA is utilized for its accessibility, stable chemical content, and prolonged efficacy. This research aims to determine the optimal concentration of vitamin B1, IBA, and their combination to enhance the growth of mother and tiller shoots of cardamom ex vitro. The research employed a two-factorial Randomized Complete Block Design, with vitamin B1 concentrations of 21.5% and 43% and IBA concentrations of 0.75 ppm and 150 ppm. The treatment V1I1 (vitamin B1 21.5% and IBA 75 ppm) on mother plant shoots maintained a survival rate of 67% up to 12 Weeks After Planting (WAP). The interaction between vitamins B1 and IBA exhibited no significant effect on all parameters of mother shoots and tillers; however, vitamin B1 significantly influenced the vegetative growth of cardamom mother shoots. Vitamin B1 at 43% produced a significantly higher number of leaves compared to 21.5%. Thus, vitamin B1 at 43% is recommended for cardamom propagation, while vitamin B1 at 21.5% has the potential to enhance the average growth of tiller shoots across all parameters.Â Â <!DOCTYPE html><html lang="en"><head><meta charset="UTF-8"><meta name="viewport" content="width=device-width, initial-scale=1.0"><title>Abstract</title><style>Â Â Â p {Â Â Â Â Â Â Â text-align: justify;Â Â Â }</style></head><body>Â Abstract. Cardamom (Amomum compactum) is an aromatic spice plant with numerous benefits, widely used in cooking, medicine, and beverages. The high demand for cardamom remains unmet due to the lengthy germination period required for cardamom seeds and the inability of cardamom shoots to thrive when planted independently from the mother plant. The presence of the mother plant significantly impacts cardamom nurseries utilizing shoots, making it challenging to obtain large quantities of nursery transplants. Growth stimulants, such as vitamin B1 and Indole Butyric Acid (IBA), are required to enÂhance vegetative growth in plants. Vitamin B1 (IPI brand) is applied due to its ease of accessibility and cost-effectiveness. Meanwhile, IBA is utilized for its accessibility, stable chemical content, and prolonged efficacy. This research aims to determine the optimal concentration of vitamin B1, IBA, and their combination to enhance the growth of mother and tiller shoots of cardamom ex vitro. The research employed a two-factorial Randomized Complete Block Design, with vitamin B1 concentrations of 21.5% and 43% and IBA concentrations of 0.75 ppm and 150 ppm. The treatment V1I1 (vitamin B1 21.5% and IBA 75 ppm) on mother plant shoots maintained a survival rate of 67% up to 12 Weeks After Planting (WAP). The interaction between vitamins B1 and IBA exhibited no significant effect on all parameters of mother shoots and tillers; however, vitamin B1 significantly influenced the vegetative growth of cardamom mother shoots. Vitamin B1 at 43% produced a significantly higher number of leaves compared to 21.5%. Thus, vitamin B1 at 43% is recommended for cardamom propagation, while vitamin B1 at 21.5% has the potential to enhance the average growth of tiller shoots across all parameters.Â </body></html>Â Â Â Â Abstract. Cardamom (Amomum compactum) is an aromatic spice plant with numerous benefits, widely used in cooking, medicine, and beverages. The high demand for cardamom remains unmet due to the lengthy germination period required for cardamom seeds and the inability of cardamom shoots to thrive when planted independently from the mother plant. The presence of the mother plant significantly impacts cardamom nurseries utilizing shoots, making it challenging to obtain large quantities of nursery transplants. Growth stimulants, such as vitamin B1 and Indole Butyric Acid (IBA), are required to enÂhance vegetative growth in plants. Vitamin B1 (IPI brand) is applied due to its ease of accessibility and cost-effectiveness. Meanwhile, IBA is utilized for its accessibility, stable chemical content, and prolonged efficacy. This research aims to determine the optimal concentration of vitamin B1, IBA, and their combination to enhance the growth of mother and tiller shoots of cardamom ex vitro. The research employed a two-factorial Randomized Complete Block Design, with vitamin B1 concentrations of 21.5% and 43% and IBA concentrations of 0.75 ppm and 150 ppm. The treatment V1I1 (vitamin B1 21.5% and IBA 75 ppm) on mother plant shoots maintained a survival rate of 67% up to 12 Weeks After Planting (WAP). The interaction between vitamins B1 and IBA exhibited no significant effect on all parameters of mother shoots and tillers; however, vitamin B1 significantly influenced the vegetative growth of cardamom mother shoots. Vitamin B1 at 43% produced a significantly higher number of leaves compared to 21.5%. Thus, vitamin B1 at 43% is recommended for cardamom propagation, while vitamin B1 at 21.5% has the potential to enhance the average growth of tiller shoots across all parameters.Â Â Â Â Abstract. Type 2 diabetes mellitus (DM) is a chronic metabolic disÂorder characterized by elevated blood glucose levels due to insulin resistance. Type 2 diabetes is considerably more prevalent than othÂer forms (85â€“90%). The risk of type 2 diabetes is higher in women (53.2%). There is an urgent requirement for better and more affordaÂble treatment options considering DM therapy is expensive and may have adverse health effects. The study's objective is to examine how acute toxicity, blood glucose levels, and body weight are affected by Moringa leaf ethanol extract (MLEE). Maceration was employed to eliminate the leaves of the moringa plant. Phytochemical screening was completed to assess the total flavonoid content and screen for alkaloids, flavonoids, phenolics, saponins, and tannins. Acute toxicity testing was performed following OECD guideline 423. Clinical sympÂtoms of acute toxicity were observed every 30 minutes for the first 24 hours post-treatment, followed by observations every 24 hours up to 14 days. The estimated LD50 range was determined. Streptozotocin/ nicotinamide-induced female Mus musculus was administered to evalÂuate the antidiabetic potential of MLEE. Six groups of mice were utiÂlized, which included a healthy control group (aquades not induced), a negative control group (induced aquades), a positive control group (induced glimepiride 0.8 mg/kg BW), and three treatment groups with varying dosages of MLEE (induced; 0, 100, and 150 mg/kg BW). A semi-auto chemical analyzer was employed on days 0 through 31 to determine blood glucose levels. An analytical digital balance was utilized to calculate the body weight. With a total flavonoid concenÂtration of 20.75%, MLEE incorporated alkaloids, flavonoids, phenoÂlics, saponins, and tannins. MLEE demonstrated a significant effect in lowering blood glucose levels at a dose of 100 mg/kg BW (P<0.05). A significant positive correlation has been identified between body weight and blood glucose levels (P<0.05).Â Â Â Â Soil arthropods play an important role in nutrient cycling and maintenance of soil structure. Thus, their abundance and diverÂsity can indicate the biological quality of the soil. Due to different soil management practices, arthropods are also very sensitive to enÂvironmental changes. This study aims to analyze the abundance and diversity of soil arthropods and the environmental factors that supÂport the abundance of soil arthropods in terrestrial of Situ Lengkong Panjalu, West Java, Indonesia. The methods used to obtain samples of soil arthropods are pitfall trap. Soil arthropod data were analyzed to determine the Shannon diversity index (H'), Margalef richness index (R), and Evenness index (E). The effect of abiotic environmental facÂtors was analyzed using Principal Component Analysis (PCA). A total of 1263 arthropods were found in Situ Lengkong Panjalu, belonging to 11 orders, 24 families, and 32 morphospecies. The current work determined the scores of H'= 2.08, R = 3,08, and E = 0.72 of soil arthropods in Situ Lengkong Panjalu, West Java, Indonesia. Results revealed that humidity is one such environmental parameter affecting the presence of soil arthropods in Situ Lengkong Panjalu, West Java, Indonesia. This research can be used as a guide in validating and conservation of the habitat of soil arthropod species on West JavaÂ Obesity has a 15-fold higher risk of coronary heart disease, stroke, and diabetes mellitus. Microalga isone of the natural resources that potentially treat obesity. The purpose of this study was to evaluate the total phenolic contents (TPC), antioxidant, and anti-obesity properties of ethanolic extract of microalgae strain MRB-2. The TPC was determined using the Follin-Ciocalteu method. The antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, and the anti-obesity was analyzed using an anti-lipase pancreatic assay. The morphology of microalga cells was also determined using Scanning Electron Microscopy (SEM). The results revealed that the TPC of ethanolic extract from the ultrasound extraction method was higher than the maceration method with the value of 2.75Â±0.26 mg GAE/g. While the scavenging activity toward DPPH radicals of ethanolic extract from the maceration method was higher than ultrasound, with a value of 38.92Â±1.94% at 0.8 mg/mL. The lipase inhibitory activity of extract from the maceration method was higher than ultrasound with a value of 20.81Â±2.24% at 0.38 mg/mL. Our results indicate that ethanolic extract of MRB-2 was potentially developed for anti-obesity foods and health-functional foods derived from new peatland microalgae. Â Talinum paniculatum Jacq (Gaertn), locally known as Javanese Ginseng, is an essential medicinal plant characterized by high flavonoid content with antioxidant and antimicrobial activities. Compared to other natural sources, a larger number of T. paniculatum leaves is required to obtain high amounts of flavonoid for drug development. In this context, in vitro culture can be used to increase flavonoid production in a controlled condition without reducing plant population. The concentration of Plant Growth Regulators (PGRs) is capable of influencing biomass and flavonoid accumulation in callus culture from several species. Despite these benefits, there is limited information about the optimal concentration of PGRs for flavonoid production from T. paniculatum callus culture. Therefore, this study aimed to investigate the optimum concentration of 2,4-D and kinetin for callus growth, as well as flavonoid production from T. paniculatum. The investigation was carried out using a completely randomized design (CRD), where young leaves explants were cultured on MS media supplemented with various concentrations of 2,4-D (0, 0.5, 1, 2) mg/L and kinetin (0, 0.5, 1, 2) mg/L for callus and flavonoid production. The results showed that the highest callus biomass of 0.105 g was produced from MS medium with 2 mg/L of 2,4-D and 1 mg/L of kinetin. Meanwhile, the highest total flavonoid content of 25.66 mg QE/g DW was produced from media supplemented with 1 mg/Lof 2,4-D and 2 mg/L of kinetin. These results showed that different combinations of 2,4-D and kinetin were required to increase biomass and flavonoid production. In conclusion, this study provided valuable information for the development of bioactive compounds through in vitro culture.Â The mistletoe (Scurrula ferruginea) is a parasitic plant obtaining nutrients and water from host plants at Nglinggo tea plantation, Kulon Progo, Yogyakarta. Despite the numerous benefits of Scurrula ferruginea leaf, substantiation regarding its pharmacological activity is still lacking. Therefore, this research aimed to identify Scurrula ferruginea species and obtain secondary metabolites with antibacterial activity. The experiment was carried out by extracting Scurrula ferruginea leaf using maceration methods with 96% ethanol, followed by qualitatively and quantitatively phytochemical screening, inhibitory zone test, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) test. Several compounds, such as flavonoids, tannins, phenols, saponins, alkaloids, terpenoids, and steroids, were identified in the mistletoe extract. The results showed total flavonoid, phenolic, and tannin levels were 7.41 Â± 0.38 mg QE/mg, 1672 Â± 50.99 mg GAE/g, and 1.43 Â± 0.15 mg TAE/g, respectively. Scurrula ferruginea leaf ethanolic extract showed the potential to form an inhibition zone against S. aureus in the moderate category at 525 mg/mL, but no significant activity was observed against E. coli. The MIC results of Scurrula ferruginea leaf ethanolic extract against S. aureus were 0.6%, and the MBC was at 2.4%. Furthermore, the MIC value against E. coli was 14%, and the MBC value was 28%. Based on these results, it could be concluded that Scurrula ferruginea leaf ethanolic extract showed high effectiveness in inhibiting and killing S. aureus bacteria compared to E. coli, with MIC and MBC values of 0.6% and 2.4%, respectively Â Grand Forest Park (Tahura) Ir. H. Djuanda, Bandung, West Java, Indonesia is a secondary nature conservation area rich in biodiversity, including macrofungi which has not been widely studied. The research aims to isolate, characterize its morphology and screen its potential as a biomaterial source based on the growth of macrofungal mycelium. The research was carried out using an exploration method at three sampling locations: Maribaya (trail area), Goa Jepang (cave area), and Curug Koleang (waterfall area). The obtained macrofungi were isolated and identified based on their macroscopic and microscopic morphological characteristics and coded based on the location and number of isolates. A comparative evaluation was carried out by one-way Analysis of variance (ANOVA) to assess the average mycelial growth of the macrofungal isolates for 9 days on PDA. The results showed that there was a total of 62 species of macrofungi from three locations: 22 isolates from the Maribaya (MB) area, 18 isolates from the Goa Jepang (GJP) area, and 22 isolates from the Curug Koleang (CK) area. The isolates that showed the highest mycelium length and represented each research area were shown by MB-07 (63.98 Â± 1.21 mm), GJP-01 (81.47 Â± 0.41 mm) and CK-13 (72.14 Â± 1.20 mm). Isolate GJP-01 from the Goa Jepang area has the potential to become a superior fungus in its ability to expand mycelium should be developed for mycelium-based material applications.Â Â Â Â Stevia rebaudiana Bert. (Stevia) is used in the commercial and health industries because of its steviol glycosides (stevioside, reÂbaudioside A, rebaudioside C) and flavonoids. Polyploidy induction of stevia plants using oryzalin was held to increase the diversity and produce superior varieties. This research aims to obtain stevia diverÂsity with different characteristics from its diploid. The TawangmanÂgu variety of stevia seedlings was experimentally designed using a completely random design. Nine combinations of oryzalin concentraÂtions (1.5, 2.5, and 3.5Î¼M) and immersion times (4, 6, and 8 hours) treatments were applied to 15 stevia seedlings each. Stevia without treatment was used as a control. Treatments were applied directly to the shoots on the second internode from the tip. Observations on tarÂget shoots included the number of survivors, morphological variables (height, number of internodes, internode length, leaf size, leaf thickÂness, stem diameter, and leaf color), stomata, and plant ploidy level. Data analysis on the number of live plants used frequency and deÂscriptively. Morphological data consisting of plant height, number of shoots, number of internodes, internode length, leaf length, leaf width, leaf thickness, and stem diameter were analyzed using boxplot graphs and descriptive to describe the diversity of M1 stevia treatment reÂsults. Leaf color and stomata were analyzed descriptively. The results showed that up to 3.5Î¼M concentrations of oryzalin and 6 hours of immersion time were safe to use as a mutation agent with above 67% survival rate. Various oryzalin treatments of Tawangmangu stevia vaÂrieties yielded polyploidy morphological growth indications in height, number of internodes, internode length, stem diameter, leaf size, leaf thickness, leaf color, stomata, and stem diameter. In addition, there are growth variations such as chimeras, rosettes, and leaf splitting. However, further flow cytometry tests showed that oryzalin concenÂtration and immersion duration directly on the vegetative material did not produce polyploid stevia individuals.

Bio-Polyurethane Resins Derived from Liquid Fractions of Lignin for the Modification of Ramie Fibers Aristri, Manggar Arum; Lubis, Muhammad Adly Rahandi; Laksana, Raden Permana Budi; Falah, Faizatul; Fatriasari, Widya; Ismayati, Maya; Wulandari, Asri Peni; Nurindah
Jurnal Sylva Lestari Vol. 9 No. 2 (2021): May
Publisher : Department of Forestry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jsl29223-238

Lignin is a biopolymer from agro-forestry biomass which provides greater prospects for higher added value applications in renewable and sustainable products. In this study, technical lignin from black liquor was used as a pre-polymer for the preparation of bio-polyurethane (Bio-PU) resins. Briefly, the isolated lignin was fractionated using ethyl acetate (EtAc) and methanol (MeOH). The liquid fractions of lignin, such as lignin-EtAc (L-EtAc) and lignin-methanol (L-MeOH), were mixed with 10% of polymeric isocyanate (based on the weigth of liquid fractions) to obtain Bio-PU resins. The isolated lignin, fractionated lignin, and lignin-derived Bio-PU resins were characterized using several techniques. The obtained Bio-PU resins then were used to modify ramie fibers using vacuum impregnation method. Fourier Transform Infrared (FTIR) spectroscopy, Differential Scanning Calorimetry (DSC), and Thermogravimetric Analysis (TGA) revealed that the isolated lignin had quite similar characteristics to lignin standar. Fractionation of lignin with EtAc and MeOH altered its characteristics. FTIR, DSC, and TGA showed that solid fractions of lignin had similar characteristics to lignin standard and isolated lignin, while the liquid fractions had characteristics from lignin and the solvents. The absorption band of isocyanate (-N=C=O) groups was shifted to 2285 cm-1 from 2240 cm-1 owing to the reaction with the -OH groups in lignin, forming urethane (R-NH-C=O-R) groups at 1605 cm-1 in Bio-PU resins. Thermal properties of Bio-PU resins derived from L-EtAc exhibited greater endothermic reaction compared to Bio-PU-L-MeOH. As a result, the free -N=C=O groups in Bio-PU resins have reacted with -OH groups on the surface of ramie fibers and improved its thermal properties. Modification of ramie fibers with Bio-PU resins improved the fibers' thermal stability by 15% using Bio-PU-LEtAc for 60 min of impregnation. Keywords: bio-polyurethane resins, impregnation, lignin fractions, ramie fibers, thermal stability

Effect of 6-Benzylaminopurine (BAP) on Apical Shoot Growth of Ramie (Boehmeria nivea L. Gaud.) Wonosobo Clone In Vitro Nuraini, Anne; Hutajulu, Esther Christy Natasya; Wulandari, Asri Peni; Elisabeth, Pauline; Muhammadiyah, Muhammadiyah
3BIO: Journal of Biological Science, Technology and Management Vol. 8 No. 2 (2026)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2026.8.2.2

Ramie (Boehmeria nivea L. Gaud.) is considered an important natural fiber crop, particularly due to its profitability. However, its propagation still faces some ultrapractical cases. When typical vegetative styles are used, the number of new shops created is relatively low, which makes large-scale production less effective. To address this conclusion, an in vitro study was conducted on the initial Wonosobo ramie clone. The aim was to determine how different concentrations of 6- benzylaminopurine (BAP) affect apical shoot conformation. Six concentrations of BAP (0, 0.5, 1.0, 1.5, 2.0, and 2.5 ppm) were examined in a completely randomized design with four replications. During the cultivation period, several responses were recorded. The study observed the time for shoot emergence, number of shoots formed, and leaf development across all treatments. The response varied across different concentrations, with certain treatments exhibiting slower shoot formation and lower quantities. The highest shoot accumulation was obtained at 1.5 ppm BAP. These results indicate that BAP is important for stimulating shoot extension, but its concentration must be carefully optimized, as both too low and too high levels are less effective. Based on these findings, BAP with 1.5 ppm is optimal for enhancing in vitro shoot production of ramie.

Co-Authors Abdul Rohmat Ade Zuhrotun Afina Yusradinan Annas Nursyahid Anne Nuraini Annisa Abdiwijaya Qaromah Annisa Handayani Dewi Aprilia Permata Sari Ayu Wandira Azmi Azhari Azmi Azhari, Azmi Baghya Nugraha Betty Mayawatie Marzuki, Betty Mayawatie Danni Ramdani Desi Harneti Putri Huspa Dewi, Annisa Handayani Dewi, Maranti Dwi Margiati Dwi Ramadhani Sukmana Elisabeth, Pauline Ema Wulandini Eva Aprilia Fadillah, Muhammad Nugraha Faizatul FALAH Faizatul Falah Fajar Fauzi Abdullah Fatih Awliya, Meuthia Felicia Fiandisty Fitri Widiantini Galih Bayu Pratama Heli Siti Halimatul Hutajulu, Esther Christy Natasya Ida Indrawati Ida Indrawati Ida Indrawati Inneke Andita Putri Laoli Irfan Zidni Ismayati, Maya Karen Kezia Lolowang Kartika Sari Kindi Farabi Kusumah, Sukma Surya Laksana, Raden Permana Budi Laoli, Inneke Andita Putri Lubis, Muhammad Adly Rahandi Manggar Arum Aristri Maya Ismayati Michelle Azista N. Casandra Mohamad Nurzaman Muhammad Adly Rahandi Lubis Muhammad Rasyidur Ridho Muhammadiyah, Muhammadiyah Murgayanti Murgayanti Nia Rossiana Nidia Primastia Ningsih, Nur Hasna Nurindah Nurindah Nurindah Oktaviani, Anti Poniah Andayaningsih Pratama, Galih Bayu Purbaya, Sari Rachmawati, Ulyaa Raden Permana Budi Laksana Rahayuningsih, Sri Redjeki Rusky Intan Pratama Sari Purbaya Sari, Aprilia Permata Shiono, Yoshihito Sukmana, Dwi Ramadhani Syifa Nabila, Devina Tati Herlina Ulyaa Rachmawati Unang Supratman Victor Handoko Vira Kusuma Dewi Wahyu Syafriansyah Widya FATRIASARI Widya Fatriasari Widya, Shafira Nurul Windra Yuniarsih Yeni Mulyani Yoshihito Shiono Yuli Andriani Yuniarsih, Windra

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