Articles
Analysis of Total Phenolic from a Root Tabar Kedayan
Siti Jubaidah;
Henny Nurhasnawati;
Heri Wijaya
Journal of Tropical Pharmacy and Chemistry Vol. 4 No. 5 (2019): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia
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DOI: 10.25026/jtpc.v4i5.205
East Kalimantan has a diversity of plants from various Dayak ethnic groups. One of them is the Tabar Kedayan (Aristolochia foveolata Merr) plant located in Malinau district. This plant is empirically efficacious as antidiarrhea, antidotum (anti poison). Efficacy of Tabar Kedayan plant has not been scientifically confirmed, this is because there is still little scientific research and information about the content of secondary metabolites and bioactive compounds contained in Tabar Kedayan Root. The presence of secondary metabolite is phenolic which has considerable biological active prospects as antioxidant, antibacterial, antiamuba, anti-inflammatory, antihepatotoxic and antivirus. The aim of this research is to analyze the chemical content and total phenolic content of root tabar kedayan in fractionation with various nonpolar, semipolar and polar solvents. The analysis used in the determination of total phenolic content using visible spectrophotometric method. Data of analysis used standard curve method based on absorbance data and concentration of standard solution. The results of this study obtained the highest total phenolic average on ethyl acetate fraction of 77.74 ±2,633 mg GAE/g then ethanol fraction of water amounted to 38.10 ±0,461 mg GAE/g and the smallest level of n-hexane fraction of 29.36±0,193 mg GAE/g. Conclusions in this study total phenolic content is found in most semipolar fractions.
PENETAPAN KADAR ASAM LEMAK BEBAS DAN BILANGAN PEROKSIDA PADA MINYAK GORENG YANG DIGUNAKAN PEDAGANG GORENGAN DI JL. A.W SJAHRANIE SAMARINDA
Henny Nurhasnawati
Jurnal Ilmiah Manuntung Vol 1 No 1 (2015): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung sekolah tinggi ilmu kesehatan samarinda
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DOI: 10.51352/jim.v1i1.7
Free fatty acids and peroxide are part of cooking oil quality parameters. This study aims to determine the levels of free fatty acids and peroxide value in cooking oil used by fried merchant in Jl. A.W. Sjahranie Samarinda. Sampling was done by total sampling which is cooking oil before frying and after frying a few times from four fried merchants. Determination of free fatty acid content using alkalimetry method and levels of peroxide using iodometric method.The test results of the free fatty acid content of samples A, B, C, D cooking oil before frying is equal to 0.16%; 0.27%; 0.33%; 0.32%, and free fatty acid levels after few times frying is 0.19%; 0.29%; 0.37%; 0.36%. The test results of the peroxide sample A, B, C, D cooking oil before frying in the amount of 18.95 meq O2/kg; 27.63 meqO2/kg; 24.67 meq O2/kg; 23.29 meq O2/kg. Peroxide levels after several times frying is 26.25 meqO2/kg; 35.72 meqO2/kg; 34.54 meqO2/kg; 33.16 meqO2/kg. Average levels of free fatty acids cooking oil before frying is 0.27% and after frying to 0.30%, or an increase of 12.04%. While the average level of peroxide cooking oil before frying of 23.64 meqO2/kg and after frying be 32.42 meqO2/kg or an increase of 37.16%.
PERBANDINGAN PELARUT ETANOL DAN AIR PADA PEMBUATAN EKSTRAK UMBI BAWANG TIWAI (Eleutherine americana Merr) MENGGUNAKAN METODE MASERASI
Hayatus Sa'adah;
Henny Nurhasnawati
Jurnal Ilmiah Manuntung Vol 1 No 2 (2015): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung sekolah tinggi ilmu kesehatan samarinda
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DOI: 10.51352/jim.v1i2.27
Bawang tiwai (Eleutherine americana Merr) is one kind of medicinal plant. To obtain an active substances with good physical and chemical properties, it is needs to do optimization of making extracts, one with solvent optimization. Type of solvent will determine the types of extracted substances in accordance with the polarity. On this research, extraction of bawang tiwai bulbs simplicia by maceration method using ethanol and water as solvent. This research is an experimental research with varying ethanol and water in a three comparison solvent that is water, ethanol, and mixture of water-etanol, then the yields is calculated. Furthermore, the data were analyzed statistically using ANOVA program to determine significant differences in the three yields. In each extract was conducted phytochemical screening to determine the content of secondary metabolites. The results showed significant differences in the yield of bawang tiwai extract with three treatments and two repetitions. The results of average yields using water as solvent is 8.75%, ethanol 5.3%; and water-etanol 8.31%
PENETAPAN KADAR PROTEIN TEMPE JAGUNG (Zea mays L.) DENGAN KOMBINASI KEDELAI (Glycine max (L.) Merill) SECARA SPEKTROFOTOMETRI SINAR TAMPAK
Siti Jubaidah;
Henny Nurhasnawati;
Heri Wijaya
Jurnal Ilmiah Manuntung Vol 2 No 1 (2016): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung sekolah tinggi ilmu kesehatan samarinda
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DOI: 10.51352/jim.v2i1.55
Indonesian is still very dependent of soybean imports, so as to reduce the use of soybean need to find substitute materials in the manufacture of soybean. Corn can be an option because apart from being a source of carbohydrates, corn is also an important source of protein in the menu society in Indonesia. The purpose of this study to determine the levels of soybean protein maize (Zea mays) with a combination of soybean (Glycine max (L.) Merrill) with visible of spectrophotometry. The research design uses a comparison of corn: soybean as follows: P1 = 80: 20; P2 = 70: 30; P3 = 50: 50; P4 = 30: 70 and P5 = 20: 80. The results of this study indicate that P1 has a protein content of 6.7%, P2 = 8.06% = 10.76% P3, P4 and P5 = 13.46% = 14.8%. The greater the concentration ratio of soy protein obtained even greater. In organoleptic quality in soybean corn combination has the texture a little hard to hard, has no smell until the typical aroma smelled soybean, flat white to brown of white
PENENTUAN KADAR RESIDU TETRASIKLIN HCl PADA IKAN AIR TAWAR YANG BEREDAR DI PASAR SEGIRI MENGGUNAKAN METODE SPEKTROFOTOMETRI ULTRA VIOLET
Henny Nurhasnawati;
Siti Jubaidah;
Novita Elfia
Jurnal Ilmiah Manuntung Vol 2 No 2 (2016): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung sekolah tinggi ilmu kesehatan samarinda
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DOI: 10.51352/jim.v2i2.64
Tetracycline HCl is one of the most commonly used antibiotics in fish farming that aims to control diseases caused by bacteria. The purpose of this study was to determine the presence and the level of tetracycline HCl antibiotic residue in freshwater fishes sold at the Segiri traditional market. The method used in this research was a standard addition ultraviolet spectrophotometry. The results showed that residue level of tetracycline HCl in freshwater fish is 192,067 μg/g – 257,409 μg/g. These result was far exceeded the maximum residue level of tetracycline class antibiotics in meat and dairy based on SNI 01-6366-2000 that limit the residue not higher than 0.1 μg/g
FORMULASI DAN EVALUASI KRIM EKSTRAK BAWANG TIWAI (Eleutherine bulbosa)
Husnul Warnida;
Henny Nurhasnawati
Jurnal Ilmiah Manuntung Vol 3 No 1 (2017): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung sekolah tinggi ilmu kesehatan samarinda
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DOI: 10.51352/jim.v3i1.93
Exposure of the skin to ultraviolet (UV) radiation from the sun has various adverse effects, photoaging and skin cancer are of great concern. The ability of botanical antioxidants for prevention of photocarcinogenesis and photoaging had been widely known. Bawang tiwai (Eleutherine bubosa) is traditionally used by the native people of Kalimantan for cancer treatment. The ethanolic extract of bawang tiwai originated from Banjarbaru district, South Kalimantan had strong antioxidant activity. The objective of the study was to formulate Eleutherine bulbosa extract into cream and evaluate its scavenging activity. Antioxidant activity of Eleutherine bulbosa cream was assessed by 2-diphenyl-1-picrylhydrazyl (DPPH) method. The Eleutherine bulbosa extract was incorporated into five cream formulas in varied concentration of emulsifying wax and cetyl alcohol. Evaluation of cream including physical appearance, pH, viscosity, stability, and microbial growth test. The result showed that Eleutherine bulbosa cream has antioxidant activity and good physicochemical quality
PERBANDINGAN METODE EKSTRAKSI MASERASI DAN SOKLETASI TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN JAMBU BOL (Syzygium malaccense L.)
Henny Nurhasnawati;
Sukarmi Sukarmi;
Fitri Handayani
Jurnal Ilmiah Manuntung Vol 3 No 1 (2017): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung sekolah tinggi ilmu kesehatan samarinda
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DOI: 10.51352/jim.v3i1.96
Malay apple (Syzygium malaccense L.) is a plant that can be used for treatment. Antioxidants have the activity to reduce free radical compounds which is one of the causes of the emergence of various diseases in humans. The purpose of this study to determine the comparison of methods of maceration and socletation extraction of antioxidant activity of malay apple leaf. Research stages include sampling, plant determination, making of simplisia, maceration extraction and socletation with 70% ethanol solvent and determine the antioxidant activity by UV-Vis spectrophotometry with DPPH (1,1-diphenyl-2-picrylhydrazyl) as free radical. The parameter is IC50 value that is the concentration of antioxidant compound which can cause 50% loss of DPPH free radical activity. Although both methods show very strong antioxidant activity, it can be concluded that the difference in extraction methods has an effect on the antioxidant activity produced. The results of antioxidant activity test showed that socletation methods gave an average IC50 value of 37.67 ppm, this value is higher than maceration methods with an average IC50 value of 47.80 ppm. Malay apple leaf has the potential as a natural antioxidant, although it has a lower IC50 value than vitamin C which is 9.72 ppm.
IDENTIFIKASI KANDUNGAN FORMALIN PADA MIE BASAH MENGGUNAKAN PEREAKSI SCHRYVER
Nurul Fatimah;
Henny Nurhasnawati;
Roufida Zaki
Jurnal Ilmiah Manuntung Vol 4 No 1 (2018): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung sekolah tinggi ilmu kesehatan samarinda
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DOI: 10.51352/jim.v4i1.147
Foods or food ingredients that contain high water content are not durable to store. This trigger manufacturers of small industries and home industries to use additional ingredients such as preservatives. Banned preservatives used in food are formalin. Identification of formaldehyde can be done by color reaction, among others, using Schryver reagent. This study aims to determine the identification of formalin content in wet noodles using Schryver reagent. This research is a type of experimental research. Sample in this research is chicken noodle and meatball noodle which is obtained from seller at Abdul Wahab Syahranie Street. The research stages include sample collection, Schryver reagent manufacture, spike sample preparation and sample testing. Data analysis used in this research use descriptive analysis method. The results of six samples of chicken noodle and meatball noodles located at Abdul Wahab Syahranie Street, that from six samples did not contain formalin. Keywords : wet noodles, formalin, Schryver reagent
PEMBUATAN STRIP TEST DAN IDENTIFIKASI FENILBUTAZON PADA JAMU PEGAL LINU
Henny Nurhasnawati;
Triswanto Sentat;
Adi Prasetiyo
Jurnal Ilmiah Manuntung Vol 6 No 2 (2020): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung sekolah tinggi ilmu kesehatan samarinda
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DOI: 10.51352/jim.v6i2.383
Jamu (Indonesian herbal medicine) are prohibited from containing chemicals medicine which are isolated or synthetic chemical compounds. Chemical compounds which is commonly added in jamu pegal linu (rheumatic pain) is phenylbutazone. One simple alternative test for detecting chemicals medicine content is to use a strip test. The purpose of this study was to determine the presence or absence of phenylbutazone content in jamu using a strip test. The research conducted is experimental research. This study used 6 samples of jamu pegal linu which were extracted using 95% ethanol solvent. The strip test is carried out by immobilizing reagents into Whatman’s filter paper using the impregnation method. Data obtained from observations of the color change of the strip test. The results showed that strip test made with alizarin red reagent and potassium hexacyanoferate (III) identified 2 samples of jamu pegal linu containing phenylbutazone, namely sample D and E, from 6 samples that circulated in Sungai Pinang District, Samarinda.
IDENTIFICATION AND DETERMINATION OF SAPONIN CONTENT FROM EXTRACT OF Embelia borneensis BARK
Titin Murtiningsih;
Risa Supriningrum;
Henny Nurhasnawati
AL ULUM: JURNAL SAINS DAN TEKNOLOGI Vol 9, No 3 (2023)
Publisher : UPT Publication and Journal Management, Islamic University of Kalimantan
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DOI: 10.31602/jst.v9i3.12499
Abstract Embelia borneensis known sekilang used by the Dayak Kenyah tribe of Long Temuyat Village, Malinau, North Kalimantan as a fish poison and leech repellent. Sekilang bark extract contains alkaloids, tannins, saponins and flavonoids compounds. The purpose of this study was to determine the type and content of saponins in the methanol extract based on different maceration, reflux, and Soxhlet extraction methods. Saponin content was determined by the gravimetric method. The results showed that the type of saponin contained in sekilang bark extract was triterpenoid saponin. The average saponin content with maceration method was 24.87%, reflux method was 28.97 and soxhlation method was 29.99%. The results of data analysis with one-way anova statistical test showed that there was a significant difference between extraction methods on saponin content of sekilang stem bark with sig value of 0.009 with a significant level of 0.05 (5%).