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EFFECT OF DPPH CONCENTRATION VARIATION ON ANTIOXIDANT ACTIVITY OF EXTRACT OF RASAM FERN LEAF (Dicranopteris linearis) Henny Nurhasnawati; Risa Supriningrum; Ardani Ardani
AL ULUM: JURNAL SAINS DAN TEKNOLOGI Vol 11, No 1 (2025)
Publisher : UPT Publication and Journal Management, Islamic University of Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31602/jst.v11i1.18488

Abstract

Dicranopteris linearis (rasam fern) is known to contain flavonoid compounds that exhibit significant antioxidant activity. In this study, the antioxidant potential of rasam fern leaf extract was assessed using the DPPH assay at three different concentrations: 30 ppm (P1), 40 ppm (P2), and 50 ppm (P3). Phytochemical screening confirmed the presence of flavonoids, tannins, and saponins in the extract. The IC50 values obtained for each treatment were as follows: P1 = 8.37 ppm, P2 = 12.31 ppm, and P3 = 13.57 ppm, all of which fall within the category of very strong antioxidant activity. Statistical analysis using one-way ANOVA revealed a significant difference in IC50 values among the three DPPH concentrations. These results indicate that variations in DPPH concentration significantly influence the IC50 values, thereby affecting the accuracy and interpretation of antioxidant activity measurements.
Aktivitas dan Stabilitas Fisik Lotion Tabir Surya dari Ekstrak Daun Rambai Warnida, Husnul; Nurhasnawati, Henny; Sukawaty, Yullia; Ardhita, Ardhita
FASKES : Jurnal Farmasi, Kesehatan, dan Sains Vol. 3 No. 1 (2025): Bulan Juli 2025, FASKES : Jurnal Farmasi, Kesehatan, dan Sains
Publisher : Program Studi Farmasi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32665/faskes.v3i1.3007

Abstract

Background: Long-term, intense sun exposure can cause erythema and sunburn, as well as premature aging and skin cancer. For generations, the people of the Banjar tribe of Borneo have applied a wet paste of Sonneratia caseolaris leaves mixture on the skin when undertaking tasks in the hot sun. The Sonneratia caseolaris tree is a mangrove plant that grows on river banks throughout the Borneo Island. Objectives: The study aims to formulate Sonneratia caseolaris leaves ethanol extract into a stable lotion and test its efficacy as a sunscreen. Methods: Sonneratia caseolaris leaves were macerate with 70% ethanol, and the sunscreen activity of the extract was examined in vitro with a UV-visible spectrophotometer, and then formulated into a lotion dosage form. Organoleptic and homogeneity observations, pH measurements, viscosity measurements, and spreadability measurements were all performed on the lotion. Following that, the lotion sunscreen activity of was tested in vitro. Results: The Sonneratia caseolaris extract sunscreen lotion has met the requirements of organoleptic, homogeneity, pH, viscosity, and spreadability tests. Conclusions and suggestions: Sunscreen lotion with 2.5% Sonnerata caseolaris extract classified as standard suntan. Sonneratia caseolaris extract of Borneo has the potential to develop as a raw material for herbal cosmetics.
Analisis Pengawet Natrium Benzoat Pada Kecap Asin Dengan Metode Spektrofotometri UV-Vis Henny Nurhasnawati; Anita Apriliana
Jurnal Farmasi Etam (JFE) Vol 3 No 1 (2023): Juni
Publisher : Unit Penelitian dan Pengabdian Kepada Masyarakat (UPPM) STIKES Dirgahayu Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52841/jfe.v3i1.246

Abstract

Kecap asin merupakan produk olahan kedelai yang digunakan sebagai penyedap masakan. Agar memiliki waktu simpan yang cukup lama, produsen umumnya menambahkan pengawet natrium benzoat, salah satu bahan pengawet yang diperbolehkan dalam bahan makanan. Penelitian ini bertujuan untuk mengidentifikasi dan menetapkan kadar natrium benzoat serta kesesuaian kadar natrium benzoat menurut SNI 01-2543-1999 dalam produk kecap asin yang beredar di Pasar Pagi Kota Samarinda. Identifikasi natrium benzoat dilakukan dengan reaksi warna menggunakan FeCl3 5%, sedangkan penetapan kadar natrium benzoat menggunakan metode Spektrofotometri UV-Vis. Analisis data dilakukan secara deskriptif. Hasil penelitian menunjukkan bahwa ketiga sampel yang diuji positif mengandung natrium benzoat ditandai dengan terbentuknya endapan kecoklatan. Kadar natrium benzoat yang diperoleh menunjukkan seluruh sampel memenuhi persyaratan SNI 01-2543-1999 yaitu tidak melebihi ambang batas 600 mg/kg. Kadar natrium benzoat pada sampel A sebesar 157,66 mg/kg ± 1,35, sampel B sebesar 148,65 mg/kg ± 0,20 dan sampel C sebesar 12,90 mg/kg ± 0,27.
KADAR FLAVONOID EKSTRAK ETANOL BUNGA TELANG (Clitoria ternatea L.) BERDASARKAN VARIASI KONSENTRASI PELARUT ETANOL Henny Nurhasnawati; Risa Supriningrum; Rusdiati Helmidanora; Margareta, Sully
Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan Vol. 11 No. 2 (2025): Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v11i2.921

Abstract

Butterfly pea (Clitoria ternatea L.) is a plant used for medicinal purposes, including therapeutic, cosmetic, nutritional, and beauty applications. Flavonoids, as secondary metabolites with diverse chemical structures in plants, perform various important functions. These functions include growth regulation, coloration, protection from UV rays, and roles in defense mechanisms and signaling systems with microorganisms. These compounds also have pharmacological effects as antidiabetics, antioxidants, and antibacterials. Solvent concentration plays an important role, as it can affect the amount of active compounds extracted from natural materials. The purpose of this study was to determine the flavonoid content in butterfly pea flower extracts using ethanol solvents at concentrations of 50%, 70%, and 95%. The results of phytochemical screening tests showed that all extracts contained secondary metabolites in the form of flavonoids. Other parameters analyzed included yield, moisture content, and flavonoid content from the three ethanol concentration variations. The yields obtained were 57.14%, 54.08%, and 40.72%, respectively, while the moisture content was 9.44 ± 2.17%, 8.99 ± 2.12%, and 7.75 ± 1.97%, respectively.The highest flavonoid content was obtained in the extract with 95% ethanol solvent at 1.2939 ± 0.0880%, followed by 70% ethanol at 0.6958 ± 0.0573%, and 50% ethanol at 0.5811 ± 0.0142%. Statistical analysis using ANOVA showed that there were differences in flavonoid content between the ethanol concentration variation groups