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All Journal Journal of Islamic Pharmacy Pharmaceutical Journal of Indonesia Lumbung Farmasi : Jurnal Ilmu Kefarmasian Hubungan Tingkat Pengetahuan Petugas Pengelola Obat dengan Tingkat Ketersediaan Obat Di Puskesmas Kota Malang PARAHITA : Jurnal Pengabdian kepada Masyarakat Jurnal Riset Farmasi Jurnal Ilmiah Sains JPMP Medical Sains : Jurnal Ilmiah Kefarmasian Jurnal Sains dan Kesehatan
Astrid Kusuma Putri
Program Studi Farmasi, Fakultas Farmasi, Universitas Hang Tuah, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Physics Public Health Social Sciences

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Determination of Total Phenolic Content of Waru Leaves Methanol and Ethyl Acetate Extracts (Hibiscus tiliaceus L.) Kusuma Putri, Astrid
Pharmaceutical Journal of Indonesia Vol. 10 No. 2 (2025)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.pji.2025.010.02.5

Abstract

Hibiscus tiliaceus L., also known as the waru plant, is a valuable source of traditional medicine due to its phenolic compounds, which have antibacterial, anti-inflammatory, antithrombotic, anticancer, and antioxidant properties. The study investigates the presence of phenolic compounds in the methanol and ethyl acetate extracts of waru leaf, which were obtained through maceration with methanol and ethyl acetate solvents, highlighting the significant impact of extraction solvent choice on the extract's active compounds. The total phenolic content in the leaf extract of waru (Hibiscus tiliaceus L.) is determined using the Folin-Ciocalteau method, in which the addition of 7% sodium carbonate creates an alkaline atmosphere that produces phenolic ions. Furthermore, it will reduce Folin to form a blue complex. The results showed that the total phenolic content of waru leaf extract (Hibiscus tiliaceus L.) was 15.348 mg GAE/g and 10.060 GAE/g of methanol and ethyl acetate extract, respectively, at a maximum wavelength of 765 nm. The repeatability precision was 1.7 x 10-14% RSD, with the gallic acid standards curve showing good linearity (R2 > 0.999).
Co-Authors Andayani, Rina Asti Rahayu Asti Rahayu Deva Oktavy American Iswardjono Dewi Perwito Sari Ersanda Nurma Praditapuspa Etik Wahyuningsih Faqihuddin Faqihuddin Fithri Farchah Frischah Sari Hardani, Prisma Trida Hardiyono Hardiyono Izazi, Farizah Kresnamurti, Angelica Lindawati Setyaningrum Liza Yudistira Yusan Lunardhi Susanto Mochamad Yuwono Nadya Ambarwati Nailufa, Yuyun Nani Wijayanti Dyah Nurrahman Praditapuspa, Ersanda Nurma Purbosari, Ira Rachma Nurhayati Rakhma, Dita Nurlita Rina Andayani Rizky Anugrah Dirgantara Putra Wahyudi Wahyudi Yanu Andhiarto Yuwono, Mochamad Yuyun Nailufa