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All Journal HAYATI Journal of Biosciences Jurnal Pengolahan Hasil Perikanan Indonesia bionature AL KAUNIYAH BERKALA SAINSTEK BioWallacea Journal of Biological Research Jurnal Sains dan Inovasi Perikanan/Journal of Fishery and Innovation (JSIPi) Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education Berkala Penelitian Agronomi Jurnal Biofiskim: Pendidikan dan Pembelajaran IPA Bioscientiae, Jurnal ilmu ilmu Biologi International Journal of Science and Environment Jurnal Pengabdian Masyarakat Nasional Majalah Pengabdian Indonesia
Sri Ambardini
Program Studi Bioteknologi, Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Halu Oleo
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary

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Genetic Diversity of Flying Fish (Exocoetidae) in Southeast Sulawesi, Indonesia Indrayani, Indrayani; Ambardini, Sri; Pariakan, Arman; Nur, Indriyani
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.744-749

Abstract

Flying fish (Exocoetidae) is a pelagic fishery commodity that holds economic value among the people of eastern Indonesia. Flying fish are advantageous as they are abundant in Indonesian sea waters, making them an affordable source of omega-3, which is beneficial for human health. However, the high demand for flying fish has resulted in overfishing, which poses a risk of reducing their natural population. Proper management of this commodity is necessary to minimize these risks. Effective management requires adequate information, including genetic diversity data. This study aims to determine the genetic diversity of flying fish in Southeast Sulawesi, specifically in the Banda Sea and Bone Bay populations. Tissue samples were collected from flying fish in the two populations during the study in March 2022. The results show that the genetic diversity values of flying fish in Bone Bay and the Banda Sea were 0.984 and 1.00, respectively, while the nucleotide diversity values were 0.021 and 0.018. High genetic diversity values and nucleotide diversity values were found between the two populations, with values of 0.990 and 0.020, respectively. Based on phylogenetic analysis, the two populations in Southeast Sulawesi are genetically similar.
Training on Processing Natural Resources into Processed Food and Health Products for Mothers in Tondongggeu Village, Nambo District, Kendari City Ambardini, Sri; Ahmad, Sitti Wirdhana; Muhsin, Muhsin; Karya, Adi; Syah, Muhamad Azwar; Adi Parman Rudia, La Ode; Yanti, Nur Arfa
Majalah Pengabdian Indonesia Vol. 1 No. 3 (2024): Desember 2024
Publisher : Teras Kampus as a member of PT Palem Edukasi Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69616/maindo.v1i3.32

Abstract

This community service activity is a training activity on the manufacture of health and food products that have the opportunity to improve the community's economy as well as providing education on the use of moringa leaves as face masks and papaya fruit as an analog tomato sauce to mothers in Tondonggeu Village, Nambo District, Kendari City. Tomato sauce is generally made from tomato raw materials, but it can also be made from other ingredients so it is called an analog tomato sauce. Tomato sauce is generally made from tomato raw materials, but it can also be made from other ingredients so it is called an analog tomato sauce. Papaya as a substitute for tomatoes in making analog tomato sauce can be done because papaya has characteristics like tomatoes, namely color, organic acid content, and viscosity, even papaya has a higher vitamin C level than tomatoes.  Moringa leaves contain many vitamins, including vitamin A, vitamin C, amino acids and antioxidants such as flavonoids, quercetin and zeatin which function to maintain dry skin hydration and prevent the appearance of wrinkles, because it helps fight free radicals, so it is very good to be used as a cosmetic ingredient in the form of a face mask. Participants in this training involved residents of Tondonggeu village, Thematic KKN students and a team of accompanying lecturers from the Biotechnology and Biology study program of FMIPA Halu Oleo University. The training methods used are lectures, demonstrations and discussions. The lecture method was carried out with the aim of providing information and knowledge about moringa masks and analog ketchup. The demonstration aims to provide skills to mothers through the practice of directly processing moringa leaves into masks and papaya fruits into analog tomato sauce while the discussion is intended to show the activeness and understanding of the community during the training process. The results of the training on making masks and analog tomato sauce carried out by mothers are in the good category with an average success of 85%, their response to the implementation of the training is very good as seen from the enthusiasm of the residents to participate in the activity from the beginning to the end. This training activity can increase knowledge and improve mothers' skills in producing moringa masks and papaya analogue tomato sauce.
Analysis of the Molecular Structure of Lipase-Dependent Chaperone from Ralstonia pickettii Strain BK6 Azwar Syah, Muhamad; Ambardini, Sri; Jamili, Jamili; Muzuni, Muzuni; Trisandy, Darul
Jurnal Biodjati Vol 9, No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33770

Abstract

Several biotechnology industries are exploring the characteristics of lipase-dependent chaperones due to their distinctive biochemical traits. This study aimed to employ bioinformatics to analyze the molecular structure of Ralstonia picketii BK6's lipase-dependent chaperon (LipRM). The sequence mapping and amino acid distribution were examined using BioEdit (version 7.0.9.1). SignalP 5.0 and Interpro are employed for signal peptide detection, whereas Swiss-Model and VMD 1.9.2 are used for molecular dynamics modelling. The results showed that the Shine-Dalgarno sequence was discovered in the LipRM promoter, seven nucleotides upstream of the initiation codon (AUG) with the 5'-AGGAGA-3', and has a terminator region that facilitates the formation of a secondary structure. The protein's 3D structure prediction results indicate differences in the alpha helix chains (residues 166-174 and 254-271) between LipRM and the reference lipase. LipRM's molecular structure comprises a detachable signal peptide, and with variations in helix alpha chain conformation and ligand geometry.
Assistance in Mastering PCR Methods for Olympiad Students at MAN Insan Cendekia in Kendari City Syah, Muhamad Azwar; Raharjo, Sapto Raharjo; Jamili; Ambardini, Sri; Jeni; Syahrir; Yamin Yaddi; Munadi, La Ode Muh
Majalah Pengabdian Indonesia Vol. 2 No. 1 (2025): April 2025
Publisher : Teras Kampus as a member of PT Palem Edukasi Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69616/maindo.v2i1.254

Abstract

Madrasah Aliyah Negeri Insan Cendekia (MAN IC) in Kendari City is one of the OSN (National Science Olympiad) participants in the subject of biology, which is required to understand the idea and practice of PCR (Polymerase Chain Reaction). However, there are insufficient skilled PCR teachers at the school, and no PCR facilities are currently accessible. The goal of this mentoring is to provide an in-depth understanding of the concept of PCR as well as full training on how to use PCR effectively. The method consists of two components: community education and training. The introduction of the PCR concept in this community service includes genetic material, DNA concepts, genes, genomes, DNA isolation, and DNA replication, while the training material includes solution taking techniques, PCR solution preparation, PCR usage procedures, PCR result visualization with electrophoresis, and data interpretation. The PCR has three main steps consisting of denaturation, annealing and extension which are carried out for 30-35 cycles with different temperatures at each step. The evaluation results showed that this mentoring helped the student understand PCR and was beneficial for the OSN competition due to its relevance to the competition syllabus. Moreover, the student also received a bronze medal in the biology competition.
Produksi protein sel tunggal mikroalga (Chlorella vulgaris) menggunakan limbah ayam broiler dan aplikasinya dalam pakan : Production of microalgae single-cell protein (Chlorella vulgaris) using broiler chicken waste and its application in feeds Iba, Wa; Padang, Crasilia Yanti; Sari, Nilam; Ambardini, Sri; Suriana, Suriana
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 27 No. 11 (2024): Jurnal Pengolahan Hasil Perikanan Indonesia 27(11)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v27i11.58151

Abstract

Protein sel tunggal dari mikroalga berpotensi digunakan dalam formulasi pakan ayam broiler. Mikroalga yang banyak dikembangkan saat ini salah satunya adalah Chlorella vulgaris. Penelitian bertujuan untuk menentukan konsentrasi C. vulgaris yang dikultur menggunakan media organik limbah ternak ayam broiler dan kandungan proteinnya dalam pakan. C. vulgaris dikultur menggunakan media organik dari feses ayam broiler dalam air laut pada 4 konsentrasi (10, 100, 1.000, dan 10.000 ppm). Kandungan protein C. vulgaris dan pakan dianalisis menggunakan metode Kjeldahl. Protein tertinggi diperoleh pada C. vulgaris dalam media feses ayam 10 ppm, yaitu 23,53%. Biomassa mikroalga yang dikultur dalam media 10 ppm feses ayam ditambahkan dalam formulasi pakan ayam broiler pada konsentrasi yang berbeda, yaitu 0; 2,5; 5; 7,5 dan 10% dengan 3 kali ulangan. Kandungan protein tertinggi, yaitu 34,65% pada penambahan biomassa C. vulgaris 2,5%. Kandungan protein yang mendekati optimal untuk ayam broiler fase pre-starter dan starter yaitu 22,71% dan 24,06% diperoleh dengan penambahan tepung C. vulgaris 5 dan 7,5%.
PEMBERDAYAAN EKONOMI MASYARAKAT PESISIR MELALUI INOVASI PENGOLAHAN IKAN MENJADI NUGGET DAN SOSIS DI KELURAHAN BUNGKUTOKO Ahmad, Sitti Wirdhana; Ardiansyah, Ardiansyah; Amirullah, Amirullah; Ambardini, Sri; Muzuni, Muzuni; Ampa, Andi Tendri
Jurnal Pengabdian Masyarakat Nasional Vol 5, No 2 (2025)
Publisher : Universitas Mercu Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22441/pemanas.v5i2.36921

Abstract

Kegiatan demonstrasi produk olahan makanan berbasis sumber daya lokal dilaksanakan di Balai Kelurahan Bungkutoko, Kecamatan Nambo, Kota Kendari, dengan tujuan untuk memberdayakan ekonomi masyarakat pesisir. Dalam pelaksanaannya, mahasiswa yang didampingi oleh tim Dosen Pembimbing Lapangan (DPL) memperkenalkan dua jenis produk olahan ikan, yaitu sosis ikan dan nugget ikan. Metode yang digunakan dalam kegiatan ini adalah ceramah dan demonstrasi langsung pembuatan produk olahan ikan. Kegiatan diikuti oleh 37 peserta yang terdiri atas anggota UMKM, ibu-ibu PKK, dan tokoh masyarakat setempat. Selama pelatihan berlangsung, peserta menunjukkan antusiasme dan partisipasi aktif dalam setiap tahapan kegiatan. Melalui kegiatan ini, masyarakat memperoleh pengetahuan dan keterampilan praktis dalam mengolah hasil perikanan menjadi produk bernilai tambah yang berpotensi menjadi sumber penghasilan tambahan. Dampak nyata dari kegiatan ini terlihat pada meningkatnya motivasi peserta, khususnya ibu rumah tangga sebanyak 90% menyatakan kesiapan untuk memproduksi olahan ikan secara mandiri dari rumah, sehingga mendukung upaya penguatan ekonomi keluarga dan pemberdayaan masyarakat pesisir secara berkelanjutan.
Bioinsecticidal Activity of Cerbera odollam Gaertn. Leaf Extract Against Thrips parvispinus Karny on Cayenne Pepper (Capsicum frutescens L.) Sri Ambardini; Eva Indraswari; Jendri Mamangkey; La Ode Adi Parman Rudia
International Journal of Science and Environment (IJSE) Vol. 6 No. 2 (2026): May 2026
Publisher : CV. Inara in Colaboration with www.stie-sampit.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51601/ijse.v6i2.611

Abstract

The increasing reliance on synthetic insecticides for pest management has raised concerns regarding environmental contamination, pest resistance, and adverse effects on non-target organisms. Consequently, the development of environmentally friendly alternatives such as botanical insecticides has gained considerable attention. This study aimed to evaluate the effectiveness of Cerbera odollam Gaertn. leaf extract against Thrips parvispinus Karny and to determine the lethal concentration required to kill 50% of the test population (LC₅₀) within 96 h of exposure. The experiment was conducted from August to October 2021 at the Biology Laboratory and Greenhouse, Faculty of Mathematics and Natural Sciences, Halu Oleo University, Indonesia. A Completely Randomized Design (CRD) was employed consisting of four extract concentrations, namely 1.0%, 1.5%, 2.0%, and 2.5%, along with a positive control (Regent 50 SC) and a negative control (distilled water). The extract was applied by spraying onto cayenne pepper plants infested with T. parvispinus, and observations were carried out for seven consecutive days. Mortality percentage, mortality rate, and LC₅₀ values were evaluated. The results demonstrated that C. odollam leaf extract significantly increased thrips mortality. The highest efficacy was observed at a concentration of 2.5%, resulting in 100% mortality and a mortality rate of 0.55 individuals per 24 h, which was statistically comparable to the positive control treatment. Probit analysis revealed an LC₅₀ value of 2.07% after 96 h of exposure. These findings indicate that C. odollam leaf extract possesses considerable potential as an environmentally sustainable botanical insecticide for controlling T. parvispinus populations in cayenne pepper cultivation.
KOMBINASI AIR KELAPA DAN EKSTRAK TOMAT PADA MEDIA GROWMORE UNTUK SUBKULTUR ANGGREK Dendrobium candy strip lasianthera (CL) Ade Putri; Rita Ningsih; Sri Ambardini
Bioscientiae Vol 23, No 2 (2026): Bioscientiae Volume 23 No 2
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v23i2.19775

Abstract

This study aims to determine the effects of individual and combined concentrations of coconut water and tomato extract as natural plant growth regulators (PGRs) on the in vitro growth of Dendrobium candy strip lasianthera (CL) plantlets cultured on Growmore subculture medium. Shoot explants originating from sterile cultures were selected, with an approximate size of 0.5 cm. The experiment employed a completely randomized design (CRD) with a two-factor treatment: tomato extract (T) concentration consisting of four levels (0, 10, 20, and 30%) and coconut water (K) at four levels (0, 50, 75, and 100 mL/L), each with five replications. Observations were conducted over an 8-week period. Data were analyzed using a Two-Way Analysis of Variance (ANOVA) followed by Duncan’s Multiple Range Test (DMRT) at a 5% significance level. The results indicated that the single treatment of tomato extract significantly affected the number of leaves and shoots. The single treatment of coconut water had a significant effect on the number of roots. Furthermore, the combination of coconut water and tomato extract significantly influenced plantlet height and shoot length. The optimal single tomato extract treatments were 30% (T3), producing an average of 3.8 leaves, and 10% (T1), producing an average of 2.6 shoots. The most effective single coconut water treatment was 100 mL/L (K3), which produced an average of 3.05 roots. The most effective combination treatments were 100 mL/L coconut water with 30% tomato extract (K3T3), resulting in a plantlet height of 1.36 cm, and 50 mL/L coconut water with 30% tomato extract (K1T3), resulting in a shoot length of 1.2 mm.
Co-Authors Ade Putri Adi Parman Rudia, La Ode Ahmad, Sitti Wirdhana Amirullah -, Amirullah Ampa, Andi Tendri Anggraini, Wa Ode Linda Rahayu ARDIANSYAH ARDIANSYAH Asrul Sani Cahyanti, Kartika Dwi Denofia Denofia DIRVAMENA BOER, DIRVAMENA Eva Indraswari Fahyuddin Fahyuddin, Fahyuddin Iba, Wa Indrawati Indrawati INDRAYANI INDRAYANI Jamili Jamili Jamili Jendri Mamangkey Jeni Kali, Yustina Rante Karya, Adi La Ode Adi Parman Rudia La Ode Iman Sulaiman La Ode Muhamad Munadi Marlina, Wa Ode Leni Muhamad Azwar Syah Muhiddin, Nurhayani Haji Muhsin Muhsin Muzuni, Muzuni Nilam Sari Nur Arfa Yanti Nur, Indriyani Nurhayani Haji Muhiddin Padang, Crasilia Yanti Pariakan, Arman Raharjo, Sapto Raharjo Ratnaen, Ratnaeni Rita Ningsih Rita Ningsih Rita Ningsih, Rita Sinta, Wa Sulaiman, La Ode Iman Suleman, Darwis Suriana Suriana Syahrir Trisandy, Darul Wirdhana, Sitti Yamin Yaddi