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Emyliana Listiowati, Emyliana
Universitas Jenderal Soedirman, Purwokerto
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PENERAPAN TEKNOLOGI PENGOLAHAN SAMPAH ORGANIK DESA SALEM BREBES MENUJU DESA MANDIRI SAMPAH Listiowati, Emyliana; Setyanto, Refius Pradipta; Ambarningrum, Trisnowati Budi; Pamudji, Ganjar; Susanto, Irwan; Lestari, Sri
Jurnal Abdi Insani Vol 12 No 2 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i2.2020

Abstract

Waste management is a global problem in Indonesia. Salem Village is one of the villages in Brebes Regency where waste management is not optimal. The condition of the increasingly piled up waste without processing creates an unpleasant odor and disturbs the health of the surrounding community. The waste processing carried out is burning the existing waste, which adds to the problem of air pollution. As a result of continuous waste burning, the hangar was also burned. The impact of burning waste is very dangerous because it releases monoxide gas which disturbs public health. The purpose of this program is to provide socialization of waste processing, waste banks, Village Regulations on waste management, waste processing technology based on BSF flies and maggots into exoenzyme products that have economic value. Activity methods include lectures and discussions, training, demonstration plots and mentoring. Socialization to cadres and administrators of Bumdes Bina Usaha Salem Village regarding a) independent waste management and waste bank applications; b) socialization of village regulations (perdes) governing waste management, c) dissemination of information about organic waste processing technology based on BSF maggots and Ecoenzymes.The results of the socialization showed an increase in participants' knowledge about the necessity of business management and group management, village regulations governing waste management, the bioecology of BSF maggots, and the technology of organic waste bioconversion using BSF maggots. The training and demonstration plots provided to partners included preparation for waste processing, ecoenzym production, baby maggot maintenance, organic waste processing techniques with BSF maggots, and prepupa harvesting.  The evaluation of the activity showed that the pre-test scores initially ranged from 25 (5.88%) to 75 (5.88%). After the technology transfer, the post-test scores increased to a range of 60 (5.88%) to 85 (11.77%).  The Community Service Program can realize the independence of the residents of Salem village in managing organic waste.
Morphological Character and Percentage of Hotate Clams (Patinopecten Yessoensis) Meat Weight in the Growing Phase, in Funka Bay, Hokkaido, Japan. faza; Hary Tjahja Soedibya, Petrus; Listiowati, Emyliana; Gyogyo, Iriyama
Journal Of Artha Biological Engineering Vol. 1 No. 1 (2023)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/7njrz078

Abstract

This study focuses on the growth and meat weight percentage of Hotate clams (Patinopecten yessoensis) during the rearing phase in Funka Bay, Hokkaido, Japan. Japan, as a major producer of seafood, including Hotate clams, plays a significant role in global seafood consumption. The objective of this study was to analyze the length-weight relationship of Hotate scallops and assess their meat weight percentage during the rearing phase. The research method involved observations using a random sampling technique during the period from August to October 2022. Samples were taken in Funka Bay, with coordinates between 42°20'-42°40' North and 140°00'-141°00' East at a depth of 43 meters. Results showed that the length-weight relationship of Hotate clams during August to October exhibited a negative allometric growth pattern, with the highest constant reaching 2.640. This means that length growth is faster than body weight. The percentage of Hotate clam (P. yessoensis) meat weight during the rearing phase ranged from 31.03% to 35.70%. This study provides important insights into the growth and meat composition of Hotate clams during critical phases in their culture in Funka Bay, Hokkaido, Japan. It can serve as a valuable information foundation for the management and maintenance of sustainable and productive Hotate clam aquaculture in the future.
Length-Weight Relationship and Condition Factors of Hotate Clams (Patinopecten yessoensis) Cultivated in Funka Bay, Japan at Different Stations Diandari, Fasya Pingka; Listiowati, Emyliana; Oktavia Kusuma, Rima
Journal Of Artha Biological Engineering Vol. 2 No. 1 (2024)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/vrppxd61

Abstract

Hotate clams (Patinopecten yessoensis) are a species of Bivalvia that is often found in the waters of Funka Bay, Hokkaido, Japan. The information regarding the relationship between length and weight and condition factors for hotate clams is important for knowing their growth patterns. This research aimed to examine the length-weight relationship and conditions of 56 Hotate clams collected in August of 2022 at two different locations. The relationship between length-weight and growth patterns shows that the range of b values at stations A and B is 2.45, and 1.86 in the waters of Funka Bay while the growth pattern in Funka Bay displays negative allometric growth with r values ranging from 0.90-0.92 (strong positive correlation). The condition factor values for Hotate clams at stations A and B are 5.45 and 1.28. There is a good welfare value for Hotate clams because the condition factor (Kn) is greater than one.
Aplikasi Budidaya Ikan Dalam Ember (Budikdamber) Di Kelompok PKK Desa Sawangan Wetan, Kecamatan Patikraja, Kabupaten Banyumas ekasanti, anandita; Dewi Nugrayani; Emyliana Listiowati; Dewi Wisudyanti Budi Hastuti; Hamdan Syakuri
Artha Imperium: Jurnal Pengabdian Kepada Masyarakat Vol. 2 No. 2 (2024): 2024
Publisher : PT. Artha Genetikalab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/fzhndj15

Abstract

Budidaya ikan dilakukan dengan menggunakan ember-emberyang dirancang untuk memelihara ikan sekaligus sayuransecara bersamaan. Tujuan kegiatan ini adalah memberikanpengetahuan dan keterampilan memelihara ikan dan sayurandalam ember kepada ibu-ibu kelompok PKK. Metode kegiatanyang digunakan adalah belajar sambil bekerja (learning bydoing). Pelaksanaan kegiatan dengan model pembelajaranorang dewasa (andragogik) dan partisipatif dalam empattahapan yang meliputi alih teknologi melaluisosialisasi/penyuluhan aplikasi budidaya ikan dalam ember,pelatihan dan percontohan, pendampingan, dan evaluasikegiatan. Hasil kegiatan menunjukkan bahwa 94,44% ibu-ibuPKK peserta pelatihan telah mengetahui teknik budidaya ikanlele dan kangkung dalam ember serta mampu untukmengaplikasikannya dengan baik. Setelah 4 minggupemeliharaan, tinggi tanaman kangkung berkisar 18-22 cmdan sudah bisa dipanen. Setelah 6 minggu pemeliharaan,berat ikan lele mencapai kisaran 38,33-55,33 gram dengantingkat kelangsungan hidup berkisar 80-85%.
GROWTH PERFORMANCE AND INTESTINAL Aeromonas spp. OF BONYLIP BARB (Osteochilus vittatus) AFTER RECEIVING DIET CONTAINING SALT AND HERBAL CONCOCTION SUPPLEMENTATION Syakuri, Hamdan; Soedibya, Petrus Hary Tjahja; Marnani, Sri; Ekasanti, Anandita; Kasprijo, Kasprijo; Kusuma, Rima Oktavia; Wijaya, Rudy; Nugrayani, Dewi; Listiowati, Emyliana; Palupi, Mustika
Indonesian Aquaculture Journal Vol 19, No 2 (2024): (December, 2024)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.2.2024.111-122

Abstract

Salt and herbal supplementations in fish feed are expected to promote aquaculture productivity, including for slow-growing fish such as Bonylip barb (Osteochilus vittatus). The objective of this study was to evaluate the growth performance and occurrence of Aeromonas spp. in the intestine of Bonylip barb fed a diet supplemented with salt and herbs. This experiment was conducted using a completely randomized design with four treatments: 1) control, 2) salt 3% supplementation, 3) herbal supplementation, and 4) Salt 3% and herbal supplementation. Growth performance evaluation included weight gain, relative growth rate, and specific growth rate after 30 and 60 days of rearing.  Bacterial samples were isolated on trypticase soy agar (TSA) and glutamate starch phenol red agar (GSP) media. Yellow colonies on GSP were counted and confirmed as Aeromonas spp. via molecular identification based on 16S rDNA sequence. Results showed that herbal supplementation, either alone or in combination with 3% salt, insignificantly increased the fish growth performance in the short-term (30 days), but tended to decrease it over the long-term (60 days). Salt supplementation consistently decreased fish growth performance but improved fish survival rates. Aeromonas isolates comprised more than 60 % of the culturable intestinal bacteria in all treatments. Furthermore, herbal supplementation appeared to increase the percentage of Aeromonas spp. in the intestine, with identified species including Aeromonas veronii, Aeromonas caviae, and Aeromonas jandaei.
ISOLASI, IDENTIFIKASI, DAN ANALISIS EKSPRESI GEN PENGKODE GROWTH HORMONE PADA IKAN SIDAT (Anguilla bicolor) Syakuri, Hamdan; Wibowo, Satrio Haryu; Ekasanti, Anandita; Soedibya, Petrus Hary Tjahja; Marnani, Sri; Listiowati, Emyliana; Nugrayani, Dewi; Sukardi, Purnama
Jurnal Riset Akuakultur Vol 19, No 1 (2024): (Maret 2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.1.2024.69-83

Abstract

Gen hormon pertumbuhan (growth hormone, GH) perlu dipelajari untuk mendukung domestikasi ikan sidat Anguilla bicolor di Indonesia. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi gen pengkode GH serta menganalisis ekspresinya pada sampel ikan sidat A. bicolor. Primer untuk amplifikasi gen GH ikan sidat A. bicolor didesain berdasarkan sekuen gen GH dari beberapa spesies ikan sidat lain yang tersedia di GenBank. Sampel cDNA hipofisa dan otak ikan sidat digunakan untuk amplifikasi gen GH. Hasil amplifikasi disekuensing dan hasilnya dianalisis menggunakan analisis BLAST, multiple sequences alignment, hormon signature, dan filogenetik. Analisis ekspresi gen GH dilakukan menggunakan teknik realtime PCR dengan metode delta-delta CT pada 14 sampel ikan sidat (23,1 ± 19,6 g; 24,5 ± 4,2 cm). Fragmen DNA sepanjang 486 bp berhasil diamplifikasi dan disekuensing. Sekuen gen GH A. bicolor memiliki similaritas nukleotida sebesar 98,49-99,14% jika dibandingkan dengan gen GH ikan sidat A. anguilla, A. australis, dan A. japonica. Sekuen parsial tersebut secara in silico diketahui mengkode bagian dari protein GH sepanjang 155 asam amino (aa). Sekuen asam amino protein GH A. bicolor sangat mirip dengan sekuen spesies ikan sidat lain dengan hanya tiga perbedaan asam amino dan membentuk satu percabangan pada pohon filogenetik. Tingkat ekspresi gen GH pada sampel ikan sidat memiliki variasi yang tinggi. Hasil penelitian ini dapat menjadi dasar untuk studi selanjutnya khususnya berkaitan dengan peran gen GH dalam pertumbuhan ikan sidat A. bicolor.The growth hormone (GH) gene needs to be studied to support the domestication of the Anguilla bicolor eel in Indonesia. This study aimed to isolate and identify the GH coding gene and analyze its expression in samples of the eel A. bicolor. Primers for amplification of the GH gene of A. bicolor eels were designed based on GH gene sequences from several other eel species available in GenBank. Pituitary and brain cDNA samples of eel were used for GH gene amplification. The amplification results were sequenced and the results were analyzed using BLAST analysis, multiple sequence alignment, hormone signature, and phylogenetic analysis. The GH gene expression analysis was carried out using the real-time PCR technique with the delta-delta CT method on 14 eel samples (23.1 ± 19.6 g; 24.5 ± 4.2 cm). The 486 bp DNA fragment was successfully amplified and sequenced. The GH gene sequence of A. bicolor has a nucleotide similarity of 98.49-99.14% when compared with the GH gene of other eel species A. anguilla, A. australis, and A. japonica. This partial sequence was found in silico to code 155 amino acids (aa) GH protein. The amino acid sequence of the A. bicolor GH protein is very similar to that of other eel species with only three amino acid differences and forms one branch on the phylogenetic tree. The expression level of the GH gene in eel samples had high variations. The results of this study could be a basis for further studies, especially regarding the role of the GH gene in the growth of the eel A. bicolor. 
PENAPISAN BAKTERI SELULOLITIK PADA SALURAN PENCERNAAN IKAN KERAPU CANTANG YANG DIBUDIDAYAKAN DI DESA BABAKAN, KECAMATAN PANGANDARAN, KABUPATEN PANGANDARAN Syukri, Ningam; ., Kasprijo; Tjahja, P.Hary; Syakuri, Hamdan; Listiowati, Emyliana
Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Vol 9 No 2 (2021): Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan
Publisher : UNIVERSITAS MUHAMMADIYAH PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (528.946 KB) | DOI: 10.29406/jr.v9i2.3000

Abstract

Ikan Kerapu Cantang merupakan hasil pesilangan dari ikan Kerapu Macan betina dan ikan Kerapu Kertang jantan. Ikan Kerapu Cantang merupakan salah satu komoditas perikanan budidaya yang memiliki nilai ekonomis tinggi. Penelitian ini bertujuan untuk mengetahui keberadaan bakteri selulolitik serta aktivitas bakteri selulolitik pada saluran pencernaan ikan Kerapu Cantang. Metode yang digunakan pada penelitian ini adalah metode observasi dengan pengambilan sampel secara purpose random sampling. Variabel utama yang diamati pada penelitian ini yaitu mengamati proporsi bakteri selulolitik dan aktivitas bakteri selulolitik pada saluran pencernaan ikan Kerapu Cantang. Untuk variabel pendukung pada penilitian ini yaitu kelimpahan bakteri, uji gram KOH, proporsi Bacillus Serta pewarnaan gram. Keberadaan bakteri Selulolitik pada saluran pencernaan ikan Kerapu Cantang pada penelitian ini ditunjukkan dengan adanya zona bening di sekeliling koloni bakteri yang ditumbuhkan pada media spesifik ( CMC 1%). Hasil penelitian ini untuk proporsi keberadaan bakteri selulolitik pada bagian anterior 49,33% middle 38,66% dan posterior 28%. Aktivitas bakteri selulolitik dari penelitian ini menunjukan hasil dengan indeks hidrolisis pada bagian anterior sebesar 0,14 – 1,4, midddle 0,1 – 3,3 dan posterior 0,2 – 1,6.
Co-Authors A. Ekasanti Agung Cahyo Setyawan Amrullah, Muhamad Anandita Ekasanti Avrila, Ririn Dwi Chairummah, Annis D. Nugrayani D. Wisudyanti Davy Ananda Catur Wibowo Dewi Fitrya Nugraheny Dewi Nugrayani Dewi Nugrayani Dewi Nugrayani Dewi Nugrayani Dewi Nugrayani Dewi Nugrayani Dewi Wisudyanti Dewi Wisudyanti Dewi Wisudyanti Dewi Wisudyanti Budi Hastuti Dewi Wisudyanti Budi Hastuti Diandari, Fasya Pingka Didi Sofiyandi Ekasanti, Anandita Faza Filda Khoirun Nikmah Gyogyo, Iriyama Hamdan Syakuri Hanafi Hanafi Hastuti, Dewi Wisudyanti Budi Hendrayana Hendrayana, Hendrayana Hendro Pramono Irwan Susanto Kasprijo, Kasprijo Kusuma, Baruna Kusuma, Rima Oktavia Laela Fitri Maheno Sri Widodo, Maheno Sri Marhaendro Santoso Mohammad Nurhafid Muh. Sulaiman Dadiono Muhammad Nurhafid Muslih Muslih Ningam Syukri Nugrayani, Dewi Oedjijono Oedjijono P.Hary Tjahja Palupi, Mustika Pamudji, Ganjar Pelamonia, Alamanda Purnama Sukardi R. Oktavia Ririn Dwi Avrila Rivani, Rivani Riviani Riviani Riviani Riviani, Riviani Setiyaningrum, Anita Putri Setyanto, Refius Pradipta Simin Simin, Simin Soedibya, Petrus Hary Tjahja Sri Lestari Sri Marnani Syakuri, Hamdan Syukri, Ningam Taufik Budhi Pramono Taufik Budhi Pramono Tjahja, P.Hary Trisnowati Budi Ambarningrum Wibowo, Davy Ananda Catur Wibowo, Satrio Haryu Widiastuti, Ekaningtyas Wijaya, Rudy Wisudyanti, Dewi Yohanes Evander