Debby Saputera, Debby
Department Of Prosthodontics, Faculty Of Dentistry University Of Lambung Mangkurat Banjarmasin

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TOXICITY TEST OF SMALL WHITE GINGER EXTRACT ON BHK-21 FIBROBLAST CELLS IN VITRO Robiansyah Robiansyah; Debby Saputera; Rahmad Arifin
Dentino : Jurnal Kedokteran Gigi Vol 5, No 1 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i1.8113

Abstract

Background: Denture stomatitis is inflammation of the oral mucosa which supporting the denture that caused by Candida albicans. Candida albicans contamination can be prevented by immersing dentures into denture cleanser solution. One of the alternative ingredients that can be used as denture cleanser is small white ginger rhizome (Zingiber officinale var. Amarum). Objective: This study aimed to analyze whether small white ginger extract (Zingiber officinale var. Amarum) was toxic to BHK-21 fibroblast cells using the MTT assay method. Method: This study was conducted in 7 groups. Five groups consisted of extracts of 30%, 40%, 50%, 60%, 70% and 2 control groups comprised of media control and cell control. Absorbance was read using ELISA reader and cell viability was calculated. Results: The percentage of living cells of all groups of small white ginger extract treatment was 100%. The parametric analysis of One Way Annova showed p = 0.498 (p> 0.05) Conclusion: Small white ginger extract (Zingiber officinale var. Amarum) is not toxic to BHK-21 fibroblast cells using the MTT Assay method because cell viability of all concentration groups is  ≥ 60%.
TOXICITY TEST OF BAY LEAF EXTRACT ON BHK-21 FIBROBLAST CELLS IN VITRO Chintya Dewi Styo Ningrum; Debby Saputera; Rahmad Arifin
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7067

Abstract

Background: Denture stomatitis is an inflammatory reaction in the oral mucosa that support the denture. This lesion is caused by the fungus Candida albicans and can be avoided by always maintain the cleanliness of denture. Bay leaf (Syzygium polyanthum) has flavonoid as the highest content which has antifungal and antioxidant properties so that bay leaf can be used as alternative ingredient for denture cleanser. Toxicity test needs to be done to determine the safety of this material. Objective: to analyze the toxicity of bay leaf extract (Syzygium polyanthum) to BHK-21 fibroblast cells using the MTT assay method. Method: This study is a true experimental study, which using posttest-only with control group design. The group, treated with bay leaf extract, were 25%, 20%, 15%, 10% and 5% concentrations including 2 control groups which were media control and cell control. Absorbance was read using ELISA reader and cell viability was calculated. Results: The percentage of living cells in all groups which treated with bay leaf extract was 100%. The parametric analysis of One Way Annova showed that there was no significant difference. Conclusion: Bay leaf extract (Syzygium polyanthum) does not have any toxic effect to BHK-21 fibroblast cell using the MTT assay method because cell viability in all treatment groups was ≥ 60%.
THE INFLUENCE BETWEEN THE LENGTH OF RADIOGRAPHER WORKING TIME AND THE REDUCTION OF SALIVARY pH (Research on Radiographers at RSUD Ulin Banjarmasin and RSGM Gusti Hasan Aman Banjarmasin in 2017) Noorma Noorma; Bayu Indra Sukmana; Debby Saputera
Dentino : Jurnal Kedokteran Gigi Vol 3, No 2 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i2.5366

Abstract

Background: Radiographer is a radiation worker in a field of investigations that uses x-ray radiation sources for health services. X-ray radiation can cause radiation effects for radiographers, patients, the environment and surrounding communities. Salivary glands are organs which susceptible to x-ray radiation. One of the effects caused by ionizing radiation is the decrease in salivary pH. Purpose: The purpose of this study was to analyze the effect of radiographers’ length of working time on salivary pH decrease due to exposure to x-ray radiation received by radiographers while working at RSUD Ulin Banjarmasin and RSGM Gusti Hasan Aman Banjarmasin. Methods: This research was an observational analytic study using cross-sectional design. The samples used in the study consisted of 18 respondents which were radiographers who did saliva taking with total sampling technique. Result: The salivary pH measurement results showed the mean value of salivary pH on the radiographer with the average of 7 years of working which has a decrease by 0.12 from the normal salivary pH of 6.70. The statistical analysis was done using parametric statistic test of Simple Linear Regression where significant value of p = 0,003. Conclusion: There is an influence between the length of radiographer working time and the reduction of saliva pH resulting from the exposure of x-ray radiation received by the radiographer while working at RSUD Ulin Banjarmasin and RSGM Gusti Hasan Aman Banjarmasin
COMPRESSIVE STRENGTH OF TYPE III GYPSUM MIXED WITH WATER OF DIFFERENT WATER HARDNESS LEVEL (Research report) Dewi Puspitasari; Suci Fikriyati; Debby Saputera
Dentino : Jurnal Kedokteran Gigi Vol 4, No 1 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i1.6172

Abstract

Background:  Fabrication of study model to replicate oral dental tissues are needed in dentistry. Type III gypsum is one of gypsum types that is often used to make cast model or die. One of type III gypsum property is compressive strength, which is an ability of the material to resist fracture. Choosing the use of water type should be considered due to final quality of the model and the die. Three different types of water based on water hardness were used in this study.Purpose: to analyze the effect of peat water, tap water and aquades water hardness to compressive strength. Method: This research was pure experimental research design with the post-test only and control design. dental  stone or type III gypsum were mixed with water from peat water, tap water and aquadest (as a control), water hardness were examined. Twenty four die stone were made to  cylinder  with size 40 mm in height and 20 mm in diameter based on ISO 6873:1998 for compressive strength test.  Result: The result of One Way Anova test and Post Hoc Bonferroni test was p= 0.000 (p <0.05)  showing a significant difference among all groups. The mean value of type III  gypsum compressive strength based on water hardness was 12.66 MPa for aquadest (soft); 9.71 MPa for tap water (hard) and 7.40 MPa for peat water (very hard). Conclusion:  there were significant differences of water hardness of peat water, tap water, and aquadest to type III gypsum compressive strength.
THE SOAKING EFFECT OF 100% SMALL WHITE GINGER EXTRACT ON THE VALUE OF THE FLEXURAL STRENGTH OF ACRYLIC BASE (Flexural Strength Value of Acrylic Base Using Acrylic Resin Heat Cured Type) Nadya Islami; Debby Saputera; Rahmad Arifin
Dentino : Jurnal Kedokteran Gigi Vol 5, No 1 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i1.8114

Abstract

Background: The denture base material that is widely used is heat cured acrylic resin. One of mechanical properties of acrylic resin that must be considered in the selection of the basic denture material is flexural strength. Flexural strength is needed, among others, to resist the chewing power that must be received by dentures. The commonly used denture cleanser is Alkaline peroxide. 100% small white ginger extract can be used as natural denture cleanser. Objective:  This study aimed to compare the flexural strength of heat cured acrylic base which in soaked 100% small white ginger extract with Alkaline peroxide solution as artificial denture cleanser. Method: This study was a pure laboratory experimental study with post test only with control group design, using simple random sampling. The sample was rectangular with a size of 65x10x2,5 mm. The number of samples used was 24 heat cured resin acrylic which was divided into 3 immersion groups, 100% small ginger extract, Alkaline peroxide, and aquades. The immersion carried out for 3 days 19 hours 25 minutes, obtained from 5 minutes denture cleanser presentation every day for 3 years. Flexural strength was tested using a Universal Testing Machine 3 Point Bending. Result: The everage flexural strength value of heat cured acrylic resin after soaked in 100% small white ginger extract was 70,98, in Alkaline peroxide 87,37, and 91,05 in aquades. The data was annalyzed using parametric One Way ANOVA test and Post Hoc Bonferroni test. Conclusion: The flexural strength of heat cured acrylic resin that soaked in 100% small white ginger extract is smaller than Alkaline peroxide after immersed for 3 days 19 hours 25 minutes.
SURFACE ROUGHNESS OF BULK-FILL RESIN-BASED COMPOSITE RESTORATIVE MATERIALS AFTER IMMERSION IN PROBIOTIC MILK Florence Brenda Fadewi; Isyana Erlita; Debby Saputera
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7061

Abstract

Background: Dentists often restore posterior cavities caused by caries using the latest resin-based composite; i.e. bulk-fill resin-based composite. Probiotic milk may affect the surface roughness because the acid content may induce degradation of filler components resulted in roughness on the surface of bulk-fill resin-based composite. Objective: This study aims to determine the surface roughness of bulk-fill resin-based composite restorative materials after immersion in probiotic milk for 10 hours and 20 hours. Method: The study applied true experimental method using pretest and posttest with control group design. The research sample was obtained using simple random sampling. This study employed 8 samples which were included in 4 treatments. Thus, the total number of samples used was 32 samples. Results: The average value of surface roughness in group I was 0.2675 µm, group II was 0.4138 µm, group III was 0.0900 µm and group IV was 0.0938 µm. The results of  One-Way ANOVA parametric test revealed p value = 0.000 (p < 0.05) and presented that the immersion of bulk-fill resin-based composite in probiotic milk for 20 hours obtained the highest average surface roughness value compared to the immersion in distilled water and probiotic milk for 10 hours. Conclusion: There is an increase in surface roughness of bulk-fill resin-based composite restorative material after immersion in probiotic milk for 10 hours and 20 hours.
HOLLOW PARTIAL DENTURE ANTERIOR RAHANG ATAS POST HEMIMAXILLECTOMY Debby Saputera; Sukaedi Sukaedi
Dentino : Jurnal Kedokteran Gigi Vol 1, No 1 (2016)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v1i1.428

Abstract

ABSTRACT Background : The hollow partial denture is a prosthesis commonly used as an effective means for rehabilitating post hemimaxillectomy patients. The size and location of the defect usually influences the extent of impairment and difficulty in prosthetic rehabilitation. Retention, stability and support are the three majors factors in the management of rehabilitation patients. Purpose : Provide information about the management of patients post hemimaxillectomy with hollow partial denture. Case : Male patient aged 40 years came to the clinic specialist Prosthodontics Faculty of Dentistry Airlangga University with complaints want to made a new maxillofacial prosthesis because the old prosthesis fracture and often loose when chewing food and to talk feel disturbed. Case Management : Print maxilla and mandible, then make bite record,  teeth arrangement and do the scraping on the palate area and some area were taken for cover hollow, acrylic procesing, reduction in denture hollow and hollow cover, hollow cover attachment process with hollow denture followed by the installation of hollow maxillary denture. Conclusion : hollow partial denture can be used as a rehabilitation post hemimaxillectomy. ABSTRAKLatar belakang : Gigi tiruan sebagian hollow adalah protesa biasa digunakan sebagai cara yang efektif untuk merehabilitasi pasien post hemimaxillectomy. Ukuran dan lokasi yang cacat biasanya mempengaruhi tingkat gangguan dan kesulitan dalam rehabilitasi prostetik. Retensi, stabilitas dan dukungan adalah tiga faktor utama dalam pengelolaan pasien rehabilitasi. Tujuan : Memberikan informasi tentang pengelolaan  pasien post hemimaxillectomy dengan gigi tiruan sebagian hollow. Kasus : Pasien laki-laki berusia 40 tahun seorang buruh datang ke klinik spesialis prostodonsia Fakultas Kedokteran Gigi Universitas Airlangga dengan keluhan ingin dibuatkan protesa maksilofasial yang baru karena protesa yang lama klamernya patah serta sering lepas saat mengunyah makanan dan untuk bicara merasa terganggu. Tata laksana kasus : Mencetak rahang atas dan rahang bawah, kemudian membuat galangan gigit, menyusun gigi dan melakukan pengerokan pada daerah palatum dan sebagian malam diambil buat penutup hollow, procesing akrilik, pengurangan pada gigi tiruan hollow dan penutup hollow, proses perlekatan penutup hollow dengan gigi tiruan hollow dilanjutkan dengan pemasangan gigi tiruan hollow rahang atas. Kesimpulan : gigi tiruan sebagian hollow dapat dipakai sebagai rehabilitasi post hemimaxillectomy.
THE IMMERSION EFFECT OF MIXTURE OF SMALL WHITE GINGER AND GARLIC EXTRACT ON COLOR CHANGES OF ACRYLIC PLATE Dea Pengasih; Debby Saputera; Bayu Indra Sukmana
Dentino : Jurnal Kedokteran Gigi Vol 5, No 1 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i1.8115

Abstract

Background: Heat cured acrylic resin is often used as a component of denture. One of the heat cured acrylic resin properties is water absorption that affects the change in acrylic color. An alternative using natural compound to minimize side effect is arising when compared to synthetic materials. A mixture of  small white ginger and garlic extract can be used as natural denture cleanser. Objective: This study aimed to know the color changes of heat cured resin plate that immersed in a mixture of 70% small white ginger and 10% garlic extract. Method: This was a true experimental study with pretest and posttest with control group design, using simple random sampling. The sample was cylindrical acrylic with 15 mm diameter and 2 mm thickness. The research was done using 18 samples heat cured acrylic resins which were divided into 3 groups, the mixture of 70% small white ginger and 10% garlic extract, alkaline peroxide, and distilled water. The color change of the sample was tested using digital analysis tools set. Results: The mean value of color changes in heat cured acrylic resin plate after immersion in a group of mixed extracts, alkaline peroxide, and distilled water were 6.23, 3.65 and 2.48. Data were analyzed using  One Way ANOVA parametric test and Dunnet T3 Post Hoc test. Conclusion: There is a difference in color changes of heat cured resin plate after the immersion in a mixture of extract and alkaline peroxide. Acrylic resin that immersed in mixed extract demonstrates a higher value of color change.
TOXICITY TEST OF GARLIC EXTRACT ON VERO CELLS IN VITRO Pandu Rana Yudhisi; Debby Saputera; widodo widodo
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7064

Abstract

Background: Denture stomatitis is an inflammation that occurs in the mucosa covered by dentures that is often attributed to increased colonization of the yeast Candida albicans that predisposes bacterial infections. Denture Stomatitis can occur due to the lack of awareness in maintaining denture hygiene. One alternative natural ingredient to serve as denture cleanser is garlic extract. Several studies have shown that garlic extract, contains flavonoids, essential oils, tannins and, mostly, allicin, exerts antibacterial and antifungal properties. Purpose: To investigate whether garlic extract (Allium Sativum L) is toxic to Vero cells using the MTT assay method. Method: This study was a purely experimental study, conducted using posttest-only with control group design. The samples were divided into 8 groups, consisted of several concentrations of garlic extract, including 0.5%, 1%, 2.5%, 5%, 7.5%, 10%, 12.5% and 15%, and 2 control groups, including control media and cell control. The absorbance was read using ELISA reader and the cell viability was calculated. Result: The value of Inhibitory Concentration (IC50) of garlic extract on Vero cells was obtained from the spss probit test that resulted in a value of 18613.782 µg/mL. Conclusion: Garlic extract does not have a toxic effect on Vero cells as indicated by the IC50 value of >1000 μg/mL.
THE EFFECT OF 25% BAY LEAF EXTRACT AND 75% SMALL WHITE GINGER EXTRACT IMMERSION IN THE COLOR CHANGE OF ACRYLIC BASE Nadia Rizky Safitri; Debby Saputera; Isnur Hatta
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7047

Abstract

Background: The most widely used denture is made from heat cured acrylic type. Acrylic resin has some disadvantages, one of which is easy to absorb liquid like water or chemical that affect color change. Alkaline peroxide is a denture cleanser that mostly sold on the market. A mixture of 25% bay leaf and 70% small white ginger extract can be used as natural denture cleanser. Objective: This study aimed to know the color change of heat cured resin base that immersed in a mixture of 25% bay leaf and 70% small white ginger extract. Method: This study was true experimental study with pretest and posttest with control group design, using simple random sampling. The sample was round with a diameter of 15mm and thickness 2mm. The samples were 18 heat cured acrylic resins which were divided into 3 groups, the mixture of 25% bay leaf and 70% small white ginger extract, alkaline peroxide, and aquades. The color change of the sample was tested using digital analysis tools set. Results: The mean value of color change of heat cured acrylic resin base after immersion in a group of mixed extracts, alkaline peroxide, and aquades were (5.22), (3.45) and (2.46). Data were analyzed using the One Way ANOVA parametric test and Bonferroni Post Hoc test. Conclusion: There is a difference in color change on the heat cured resin after immersion in a mixture of extract and alkaline peroxide. The value of acrylic resin that immeresed in mixed extract has the higher color change.