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Antibiofilm activity of Cyanobacteria spirulina as an irrigation solution against Enterococcus faecalis Soesilo, Diana; Pangabdian, Fani; Juniar, Eriza
Majalah Kedokteran Gigi Indonesia Vol 9, No 1 (2023): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.78246

Abstract

Sodium hypochlorite (NaOCl) is currently the golden standard for root canal irrigation. NaOCl at a concentration of 5.25% to 6% can eliminate E. faecalis, but this concentration can increase the risk of toxic effects. Cyanobacteria spirulina is known to produce several secondary metabolites that have antimicrobial activity against gram-positive and gram-negative bacteria. The aim of this study was to determine the antibiofilm power of Cyanobacteria spirulina against the biofilms of Enterococcus faecalis at concentrations of 60 mg/ml, 70 mg/ml, 80 mg/ml, and 90 mg/ml. This research was a true experiment with a post-test only group design. The object of the research was divided into 6 groups. Group 1 was a negative control group, group 2 was a positive control group with 5% NaOCl, group 3, 4, 5, 6 were treatment groups given Cyanobacteria spirulina solution at a concentration of 60 mg/ml, 70 mg/ml, 80 mg/ml, and 90 mg/ml, respectively. Optical density (OD) of bacteria was bound by staining and analyzed by ELISA auto reader with a wave length of 595 nm (OD 595 nm). The results of the LSD test showed that the significance between K+ and P1, P2, P3 (p< 0.005), K+ and P4 (p= 0.129), P1 and P2 (p=0.449), P3 and P4 (p=0.178). Significance of p<0.0005 showed a significant difference between the groups. The data were analyzed using the one-way ANOVA test followed by a double comparison test with the Least Significance Different (LSD) Post Hoc test method. Cyanobacteria spirulina solution at a concentration of 90 mg/ml had the greatest inhibitory effect on the biofilm formation of Enterococcus faecalis.
Sitotoksisitas komposit serat selulosa sabut kelapa sebagai kandidat novelty basis pada material kedokteran gigi: studi eksperimental cevanti, Twi agnita; Soesilo, Diana; Pangabdian, Fani; Wijaya, Yongki Hadinata; Puspita, Sinta; Hollanda, Ghita Hadi
Padjadjaran Journal of Dental Researchers and Students Vol 7, No 2 (2023): Juni 2023
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v7i2.46092

Abstract

ABSTRAKPendahuluan: Teknologi Fiber-reinforced composite (FRC) sedang dikembangkan sebagai bahan inovatif dalam kedokteran gigi. Menipisnya sumber daya fosil merupakan masalah kritis untuk resin komposit serat saat ini. Sabut kelapa Cocos nucifera L.(coir) memiliki potensi tinggi untuk menggantikan bahan penguat serat sintetis sebagai bahan baru yang akan dikembangkan. Tujuan penelitian mengetahui toksisitas komposit serat selulosa coir sebagai material basis. Metode: Jenis penelitian eksperimental post-test only control group. Selulosa dari coir disintesis menggunakan organosolvent, di bleaching dengan peroxide dalam larutan alkali, serta di nukleasi dengan etanol absolut. Dilakukan uji viabilitas sel fibroblas GT1 untuk serat selulosa kemudian pembuatan komposit selulosa coir dengan fraksi berat 70% filler serat selulosa, dan 30% matriks BisGMA, TEGDMA dan DGEBA. Selanjutnya komposit selulosa coir dilakukan uji toksisitas dari hasil rendaman komposit pada saliva buatan selama 7,14, dan 21 hari menggunakan sel fibroblas BHK-21. Hasil: Hasil uji Post Hoc LSD memberikan kesimpulan bahwa kelompok P-21 didapatkan perbedaan yang signifikan antara kelompok perlakuan K.Sel, P-7, P-14 dengan nilai p<0,05. Dosis paling aman serat selulosa coir sebesar 12,5mg/ml; hasil uji Kruskal-wallis dari lama perendaman komposit terdapat perbedaan yang bermakna antar kelompok dengan nilai signifikansi p<0,05 (p=0,001). Simpulan: Serat selulosa coir tidak toksik sehingga dapat menjadi filler komposit sebagai salah satu prasyarat untuk menjadi kandidat novelty basis pada material komposit kedokteran gigi.KATA KUNCI: coir, komposit, toksistas, basis kedokteran gigiCytotoxicity of cellulose fiber from coconut coir as a novelty base candidate on dental materials: experimental studyABSTRACTIntroduction: Fiber-reinforced composite (FRC) technology is being developed as an innovative material in dentistry. The depletion of fossil resources is a critical issue for current fiber composite resins. Cocos nucifera L. coconut fiber (coir) has high potential to replace synthetic fiber reinforcement as a new material to be developed. The aim of the study was to determine the toxicity of coir cellulose fiber composites as a base material. Methods: This was a post-test only control group experimental study. Cellulose from coir was synthesized using organosolvent, bleached with peroxide in alkaline solution, and nucleated with absolute ethanol. GT1 fibroblast cell viability test was conducted for cellulose fibers and then coir cellulose composites were made with a weight fraction of 70% cellulose fiber filler, and 30% BisGMA, TEGDMA and DGEBA matrix. Furthermore, cellulose coir composites were tested for toxicity from the results of composite immersion in artificial saliva for 7, 14, and 21 days using BHK-21 fibroblast cells. Results: The results of the Post Hoc LSD test concluded that the P-21 group had a significant difference between the K.Sel, P-7, P-14 treatment groups with a value of p<0.05.The safest dose of coir cellulose fiber is 12.5mg/ml; the results of the Kruskal-wallis test of the composite soaking time have significant differences between groups with a significance value of p<0.05 (p=0.001). Conclusion: Coir cellulose fiber is non-toxic so it can be a composite filler as one of the prerequisites to be a novelty base candidate in dental composite materials.KEY WORDS: coir, composite, toxicity, dental base
Pengaruh Budaya, Komitmen Organisasi, dan Kepuasan Kerja Terhadap Kedisiplinan Perawat Gigi (Rumah Sakit Gigi dan Mulut Pendidikan Nala Husada Surabaya) Pangabdian, Fani; Purwadhi, Purwadhi; Handayani, Nining; Aisyah Fitri, Nurul
Jurnal Manajemen dan Administrasi Rumah Sakit Indonesia (MARSI) Vol 8, No 4 (2024): Jurnal Manajemen dan Administrasi Rumah Sakit Indonesia (MARSI)
Publisher : LPPM Universitas Respati Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52643/marsi.v8i4.4629

Abstract

Perbedaan status perawat gigi di RSGMP Nala Husada dapat diklasifikan menjadi dua yaitu perawat gigi BKO (Bawah Kendali Operasi) dan perawat gigi Organik. Perbedaan status pegawai tersebut berdampak terhadap pembagian tugas pokok hingga jadwal pemberian salary, sehingga memicu terjadinya konflik internal di dalam organisasi. Penelitian ini bertujuan untuk mengetahui pengaruh dari budaya, komitmen organisasi, dan kepuasan kerja terhadap kedisplinan perawat gigi di RSGMP Nala Husada. Jenis penelitan ini merupakan penelitian kuantitatif dengan jumlah responden 36 responden. Teknik pengumpulan data menggunakan purposive sampling dengan kuesioner. Metode analisis data dalam penelitian ini menggunakan regresi linier berganda menggunakan alat bantu SPSS. Hasil penelitian menunjukkan bahwa variabel budaya organisasi dan kepuasan kerja berpengaruh signifikan terhadap kedisplinan perawat gigi RSGMP Nala Husada (0,019 dan 0,001). Sedangkan variabel komitmen organisasi tidak berpengaruh signifikan terhadap kedisplinan (0,152). Namun secara simultan ketiga variabel tersebut (budaya, komitmen organisasi dan kepuasan kerja) berpengaruh signikan terhadap kedisiplinan kerja dengan nilai sig 0,000 (< 0.05).
PREVENTION OF DENTAL CARIES THROUGH DENTAL HEALTH EDUCATION AND UTILIZATION OF MARINE PRODUCTS AT ELEMENTARY SCHOOL MUHAMMADIYAH 9 SURABAYA Pinasti, Restika Anindya; Pangabdian, Fani; Damayanti, Nurul
Abdi Dosen : Jurnal Pengabdian Pada Masyarakat Vol. 9 No. 3 (2025): SEPTEMBER
Publisher : LPPM Univ. Ibn Khaldun Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/abdidos.v9i3.3032

Abstract

Muhammadiyah 9 Elementary School, Surabaya, is geographically located on the coast, so it is often referred to as Bahari Muhammadiyah 9 Elementary School. Based on preliminary survey results, Muhammadiyah 9 Elementary School students frequently consume cariogenic foods, resulting in a high caries index of 4.6. From an educational perspective, the learning method is still relatively conventional and does not align with the "Merdeka Belajar" curriculum, resulting in hampered learning and a lack of student knowledge about maintaining health, especially dental and oral health. Furthermore, the implementation of school dental health activities has not been optimal. Community service methods are carried out through several stages: outreach, training, mentoring and evaluation, and a desire program. The participation of the university community in helping solve the problems facing the community in such environments is essential by providing education and regular dental and oral health checks to reduce the caries index, as well as providing snacks such as sea cucumber jelly candy and anchovy cookies made from seafood, which have anti-cariosis properties. The use of seafood as an alternative to prevent dental caries shows very promising potential. Program Pengabdian kepada Masyarakat Bima 2025: This program has succeeded in raising student awareness of the importance of maintaining dental hygiene and introducing innovative and natural ways to prevent caries.
TRAINING OF TRAINER (TOT) DALAM UPAYA PEMELIHARAAN KESEHATAN GIGI UNTUK ORANG TUA DAN SISWA DI SD RADEN PAKU SURABAYA Rahmitasari, Fitria; Rosita Irmawati, Ari; Revianti, Syamsulina; Nilawati, Nina; Widyastuti; Kurniawan, Hansen; Bayu Prabowo, Puguh; Pangabdian, Fani; Nur Ashrin, Meinar; Widaningsih; Handayani, Budi; Khoironi, Emy; Lukisari, Cane; Samadi, Karlina; Harnowo, Setyo
Jurnal Pengabdian Masyarakat Pesisir VOLUME 3 NOMOR 2
Publisher : Universitas Hang Tuah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpmp.v3i2.120

Abstract

Angka kejadian permasalahan kesehatan gigi dan mulut di Indonesia mencapai 57,6%, dimana tergolong sangat tinggi, dengan 93% di antaranya dialami oleh anak-anak usia sekolah. Pengetahuan, sikap, dan perilaku individu memiliki peran yang penting dalam menjaga kesehatan gigi mulut. Lingkungan sekitar anak, terutama keluarga dan guru, memiliki peran yang krusial dalam membentuk sikap dan perilaku mereka. School Health Program (SHP) yang melibatkan pelatihan Training of Trainer (ToT) mengenai kesehatan gigi mulut bagi guru dan orang tua mencakup kegiatan menyikat gigi dua kali sehari selama 21 hari. Program ini bertujuan untuk memberikan pendidikan kepada anak dan orang tua beserta guru tentang perlunya menjaga oral hygiene yang baik serta mengajarkan teknik menggosok gigi dengan pasta gigi berfluoride. Inisiatif ini juga mendukung pencapaian target "Indonesia Bebas Karies 2030" dan dilaksanakan secara daring melalui aplikasi Zoom. Pemahaman peserta terkait kesehatan gigi dievaluasi melalui kuesioner sebelum dan sesudah diberikan pelatihan. Analisis statistik menggunakan uji t-sampel berpasangan untuk mengetahui dan membandingkan nilai rerata sebelum dan sesudah diberikan pelatihan menunjukkan nilai p<0,05, yang mengindikasikan adanya peningkatan pengetahuan yang signifikan mengenai pemeliharaan kesehatan gigi mulut pada guru serta orang tua siswa di SD Raden Paku melalui program ToT. Kegiatan ini terbukti efektif dalam meningkatkan pengetahuan dan perilaku mengenai kesehatan gigi dan mulut setelah materi diberikan. 
NATIONAL DENTAL HEALTH MONTH PROGRAM 2022 SCHOOL HEALTH PROGRAM WITH ITS KINDERGARTEN Revianti, Syamsulina; Brahmanta, Arya; Agnita Cevanti, Twi; Puspita, Sinta; Khoironi, Emy; Pangabdian, Fani; Widaningsih, Widaningsih; Hanum, Faizah; Listya Paramita, Annisa; Dwi Maharani, Aulia; Paramita, Ayulistya; Iman, Dimas; Rizal, Mohammad Basroni; Anindya Pinasti, Restika; Soesilo, Diana
Abdi Dosen : Jurnal Pengabdian Pada Masyarakat Vol. 7 No. 3 (2023): SEPTEMBER
Publisher : LPPM Univ. Ibn Khaldun Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/abdidos.v7i3.1835

Abstract

Dental and oral health in Indonesia is still a health problem that needs attention. The most concerning thing is that as many as 93% of school-age children experience dental and mouth problems. This can be caused by a lack of knowledge and awareness of dental hygiene. One of the efforts made to increase knowledge and awareness of dental hygiene is by holding an educational activity called the "School Health Program". This activity was carried out in several schools, one of which was TK ITS. This activity began with the Training of Trainer Program given by dentists to teacher cadres and parents of students/students/students about the benefits of good oral hygiene and how to brush your teeth twice a day with fluoride toothpaste. Dentists provide educational materials using power point media and educational videos through the zoom application. There were 5 cadres consisting of 2 teachers and 3 guardians of students who attended this activity. After that, the teacher cadres and parents of the students/students disseminate information to other parents of students/students so that they can become mentors to their children to apply good and correct behavior of brushing their teeth twice a day for 21 days with the method recorded on the given calendar. The hope is that after this program ends, students at ITS Kindergarten have the behavior of maintaining oral hygiene by brushing their teeth twice a day with fluoride toothpaste.