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Antifungal effect of Sticophus hermanii and Holothuria atra extract and its cytotoxicity on gingiva-derived mesenchymal stem cell Kristanti Parisihni; Syamsulina Revianti
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 4 (2013): December 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (346.243 KB) | DOI: 10.20473/j.djmkg.v46.i4.p218-223

Abstract

Background: Sea cucumber had been acknowledged to have some medical properties Sticophus hermanii and Holothuria atra are species of sea cucumber which has been known to have antifungal properties thus potentially explored as therapeutic agent in oral candidiasis. Purpose: The aim of this study was to examine the antifungal property Sticophus hermanii and Holothuria atra extract against Candida albicans and its cytotoxicity to human gingiva-derived mesenchymal stem cell. Methods: The study was an experimental laboratories research with post test only control group design. Methanolic extract of Sticophus hermanii and Holothuria atra in concentrations of 1%, 0.5%; 0.25%; 0.13%, 0.07%; 0.03%, 0.02% and 0.01%; were tested its cytotoxicity on gingiva-derived mesenchymal stem cell. Cell viability were measured by MTT assay. The antifungal property against Candida albicans was tested by disk diffusion method. Data were analyzed by ANOVA followed by LSD. Results: Extract of Sticophus hermanii showed no cytotoxicity in all concentrations (p>0.05), while Holothuria atra showed toxicity in the concentration of 1% and not cytotoxic in the concentrations below (p<0.05). Both sea cucumber extract could inhibit the growth Candida albicans, in vitro, proved by the clear zone around the disc in all concentrations (p<0.05). Conclusion: Stichopus hermanii and Holothuria atra extract had the antifungal effect against Candida albicans. Sea cucumber extract were not cytotoxic togingiva-derived mesenchymal stem cell in the concentration of Sticophus hermanii ≤ 1% and Holothuria atra ≤ 0.5%.Latar belakang: Teripang telah diketahui mempunyai berbagai khasiat medis. Sticophus hermanii dan Holothuria atra adalah spesies teripang yang telah diketahui mempunyai sifat anti jamur sehingga santat potensial untuk diekplorasi sebagai agen terapeutik pada infeksi di rongga mulut. Tujuan: Tujuan dari penelitian ini adalah untuk meneliti sifat anti jamur ekstrak Sticophus hermanii and Holothuria atra terhadap Candida albicans dan sitotoksisitasnya terhadap stem sel mesenkimal yang berasal dari gingiva manusia. Metode: Penelitian ini merupakan penelitian eksperimental laboratoris dengan rancangan post test only control group design. Ekstrak metanol Sticophus hermanii dan Holothuria atra pada konsentrasi 1%, 0,5%; 0,25%; 0,13%, 0,07%; 0,03%, 0,02% and 0,01% diuji sitoksisitasnya terhadap stem sel mesenkimal yang berasal dari gingiva. Viabilitas sel diukur dengan menggunakan metode MTT. Sifat anti jamur terhadap Candida albicans diuji dengan metode difusi. Data dianalisis dengan ANOVA dan LSD. Hasil: Ekstrak Sticophus hermanii tidak menunjukkan adanya toksisitas pada seluruh konsentrasi (p>0,05), sedangkan Holothuria atra menunjukkan adanya toksisitas pada konsentrasi 1% dan tidak toksik pada konsentrasi di bawahnya (p<0,05). Kedua ekstrak mampu menghambat pertumbuhan Candida albicans in vitro yang ditunjukkan dengan adanya zona jernih disekitar disk pada semua konsentrasi (p<0,05). Simpulan: Ekstrak Stichopus hermanii dan Holothuria atra mempunyai daya anti jamur terhadap Candida albicans. Ekstrak teripang bersifat tidak toksik terhadap stem sel mesenkimal yang berasal dari gingiva masing-masing pada konsentrasi Sticophus hermanii ≤ 1%, dan Holothuria atra ≤ 0,5%.
The activity of Stichopus hermanii extract on triglyceride serum level in periodontitis Rima Parwati Sari; Syamsulina Revianti
Dental Journal (Majalah Kedokteran Gigi) Vol. 44 No. 2 (2011): June 2011
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (742.562 KB) | DOI: 10.20473/j.djmkg.v44.i2.p106-110

Abstract

Background: The level of triglyceride can be used as a parameter of  hypercholesterolemia. Periodontitis can make the condition of hypercholesterolemia worse. Stichopus hermanii extract is a source of saturated fatty acid containing omega-3 which can decrease triglyceride blood level. Purpose: The aim of this research was to investigate the effect of Stichopus hermanii extract in triglyceride blood level of wistar rats which got periodontitis. Methods: The samples of this research were 30 rats divided into 5 groups, namely group K(–) as negative control group (without treatment), group K(+) as positive control group (induced with periodontopathogen mix), group P1 as treatment group1 (induced with periodontopathogen and Stichopus hermanii extract mix, 0.09 ml/kgW), Group P2 (induced with periodontopathogen and Stichopus hermanii extract mix, 0,18 ml/kgW), and group P3 (induced with periodontopathogen and Stichopus hermanii extract mix, 0,36 ml/kgW). Then, all of those rats were sacrificed and all serum was measured for their level of triglyceride. Results: All data was analyzed with ANOVA test showing a significant result. LSD test showed a significant different between group K(–) and group K(+), and between group K(+) and group P2 and P3. Conclusion: Stichopus hermanii extract can decrease the triglyceride blood level in wistar rats with periodontitis.Latar belakang: Kadar trigliserida dalam darah dapat digunakan sebagai parameter hiperkolesterolemia. Periodontitis dapat memperburuk kondisi hiperkolesterolemia. Stichopus hermanii ekstrak mengandung asam lemak jenuh terutama omega-3 yang dapat berfungsi menurunkan kadar trigliserida dalam darah. Tujuan: Tujuan dari penelitian ini adalah untuk mengetahui pengaruh ekstrak Stichopus hermanii terhadap kadar trigliserida dalam darah tikus wistar yang mengalami periodontitis. Metode: Sampel penelitian ini adalah 30 ekor tikus wistar yang dibagi dalam 5 kelompok. Kelompok K(–) sebagai kelompok kontrol negatif (tanpa perlakuan), kelompok K(+) sebagai kelompok kontrol positif (tikus diinduksi periodontopatogen campuran), kelompok P1 sebagai kelompok perlakuan 1 (tikus diinduksi periodontopatogen campuran dan Stichopus hermanii ekstrak 0,09 ml/kgW), kelompok P2 (tikus diinduksi periodontopatogen campuran dan Stichopus hermanii ekstrak 0,18 ml/kgW) dan P3 (tikus diinduksi periodontopatogen campuran dan Stichopus hermanii ekstrak 0,36 ml/kgW). Kemudian semua tikus dikorbankan dan diukur kadar trigliserida serumnya. Hasil: Semua data dianalisis dengan uji ANOVA dan menunjukkan hasil yang signifikan, dan uji LSD menunjukkan terdapat perberbedaan yang bermakna antara kelompok K(–) dengan K(+) dan kelompok K(+) dengan P2 dan P3. Kesimpulan: Ekstrak Stichopus hermanii dapat menurunkan kadar trigliserida darah pada tikus wistar yang mengalami periodontitis.
Potency of Stichopus hermanii extract as oral candidiasis treatment on epithelial rat tongue Syamsulina Revianti; Kristanti Parisihni
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 1 (2016): March 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (700.245 KB) | DOI: 10.20473/j.djmkg.v49.i1.p10-16

Abstract

Background: Oral candidiasis is the most prominent oral fungal infection with Candida albicans (C. albicans) as 75% of ethiologic factor. Golden sea cucumbers (Stichopus hermanii) have been consumed by Asian community as folk medicine. It has been known to have antifungal and immunomodulator agent thus potential to be explored as treatment in oral candidiasis. Purpose: The aim of this study was to examine the potency of Stichopus hermanii extract as oral candidiasis treatment. Method: The study was an experimental laboratories research with post test only control group design. Thirty male wistar rats were divided into 5 groups i.e negative control, positive control and 3 treatment groups. Oral candidiasis condition were induced by spraying C. albicans suspension on dorsal tongue of wistar rats, once in 2 days for 14 days. The treatment groups were given Stichopus hermanii extract on the dose of 4.25 ml/kgBW, 8.5 ml/kgBW, 17 ml/kgBW once daily for 14 days. The expression of anti C. albicans antibody and TNF-α were examined by immunohistochemistry on ephitelial tongue. Data was analyzed by Manova and LSD test. Result: Anti C. albicans antibody expression were higher in positive control group than in negative control group while TNF-α expression were lower in positive control group than in negative control group (p<0.05). Treatment with Stichopus hermanii extract on all doses decreased the expression of anti C. albicans antibody and increased the expression of TNF-α (p<0.05). Conclusion: Stichopus hermanii extract decreased the expression of anti C. albicans antibody and increased the expression of TNF-α in epithelial rat tongue.
The effects of Anadara granosa shell-Stichopus hermanni on bFGF expressions and blood vessel counts in the bone defect healing process of Wistar rats Rima Parwati Sari; Sri Agus Sudjarwo; Retno Pudji Rahayu; Widyasri Prananingrum; Syamsulina Revianti; Hansen Kurniawan; Aisah Faiz Bachmid
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 4 (2017): December 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (578.577 KB) | DOI: 10.20473/j.djmkg.v50.i4.p194-198

Abstract

Background: Bone damage can be caused by various factors with treatment usually involving graft materials being applied to the defective area. Moreover, in the bone defect healing process, blood vessels are also considered to be an important energy source for cell proliferation. One of the angiogenic factors playing an important role in blood vessel formation is basic fibroblast growth factor (bFGF). Furthermore, synthesized hydroxyapatite derived from Anadara granosa (AG) shells constitutes one of the potential materials for use in bone graft. The gold sea cucumber genus Stichopus hermanni (SH) possesses the ability to stimulate endothelial progenitor cells inducing bFGF. Purpose: This study aims to investigate the effects of AG shells and SH on bFGF expressions and blood vessel counts within the bone healing process. Methods: Twenty four male Wistar rats were divided into three groups, namely: a control group (C), a treatment group was administered with blood cockle shell (AG), and a treatment group with blood cockle shell and golden sea cucumber (AG+SH). Defects were made on their femurs measuring half the diameter of a circular, no. 018. bur These rats were subsequently sacrificed on day 7 after surgery. The expressions of bFGF were measured by means of IHC technique, while the number of blood vessels was quantified using HE technique. The resulting data was subjected to statistical analysis using an Anova test followed by an LSD test (p < 0.05). Results: The one-way Anova test results combined with those of an LSD test showed there to be significant differences in bFGF expressions and blood vessel counts between the control group (K) and the treatment group (AG) as well as between the treatment group (AG) and the treatment group (AG+SH). Conclusions: A combination of Anadara granosa shell and Stichopus hermanni can increase the expression of bFGF and the number of blood vessels on day 7 during the bone healing process in Wistar rats.
Diet Bubuk Cangkang Anadara Granosa dan Susu Kedelai Meningkatkan Kekerasan Permukaan Gigi Rima Parwati Sari; Syamsulina Revianti; Puguh Bayu Prabowo
Jurnal Material Kedokteran Gigi Vol 1 No 1 (2012): JMKG Vol 1 No 1 Maret 2012
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.204 KB)

Abstract

Wistar teeth always be growth, but to remodeling needed some minerals and protein to accelerate enamel and dentin to be hardness. Anadara granosa cockle shell showed that it contains a lot of minerals, majority calcium that is important to mineralized of teeth. In the absorption of calcium need amino acid as protein-binding transporter that found in soya milk. The aim of this study was to proved diet combination Anadara granosa cockle shell powder and soya milk increased surface hardness wistar teeth. Twenty eight (28) wistar rats were divided four groups. 1st group was given aquadest, 2nd (Anadara granosa cockle shell powder 0,1368 mg/grBB), 3rd (soya milk 0,015 ml/grBB), 4th (Anadara granosa cockle shell powder 0,1368 mg/grBB and soya milk 0,015 ml/grBB). On 12nd day tooth incisors sample was extracted and done measurement by microhardness vickers. All of datas in this study were analyzed by ANOVA and LSD (p=0,05). This study showed that there was significant difference (p>0.05) between 2nd and 4th group with 1st and 3rd groups so it can be cancluded that the use of diet combination Anadara granosa cockles shell powder and soya milk can increased surface hardness of wistar teeth.
EDUKASI KESEHATAN GIGI DAN MULUT SERTA ORAL SCREENING PADA ANAK-ANAK PANTI ASUHAN MAHBUBIYAH SURABAYA Bunga Fauzia; Arya Brahmanta; Nora Lelyana; Kristanti Parisihni; Syamsulina Revianti; Dwi Setianingtyas; Dimas Iman; Nafiah Nafiah; Shintya Rizki Ayu Agitha; Oka Lestari
Jurnal Pengabdian Kepada Masyarakat Sakai Sambayan Vol 7 No 2 (2023)
Publisher : Lembaga Penelitian dan Pengabdian Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jss.v7i2.404

Abstract

Pemberian edukasi tentang kesehatan gigi dan mulut bertujuan untuuk meningkatkan pengetahuan dan cara pemeliharaan kesehatan gigi dan mulut khuusnya pada anak-anak Panti Asuhan Mahbubiyah Surabaya. Beberapa dari mereka kurang pengetahuan tentang bagaimana cara menjaga kesehatan gigi dan mulut yang baik sehingga diperlukan kegiatan pengabdian masyarakat dalam peningkatan pengetahuan pemeliharaan kesehatan gigi dan mulut agar terhindar dari kerusakaan gigi dan mulut yaitu berupa pemberian edukasi dan pemeriksaan rongga mulut anak anak Panti Mahbubiyah Surabaya. Pemeriksaan gigi dan mulut (oral screening) pada anak anak Panti Asuhan Mahbubiyah dilakukan untuk mendeteksi sejak dini kerusakan gigi dan mulut yang terjadi pada anak-anak. Pemberian edukasi kesehatan gigi dan mulut dilakukan dengan menggunakan bantuan alat peraga berupa phantom gigi dan poster. Setelah pemberian edukasi, anak anak diberikan beberapa pertanyaan langsung untuk mengetahui tingkat pengetahuan anak anak tentang pemeliharaan kesehatan gigi dan mulut. Kemudian dilanjutkan dengan pemeriksaan kesehatan gigi dan mulut pada anak anak. semua anak di panti asuhan sangat kooperatif dan mau diperiksa kondisi rongga mulutnya. Dengan adanya kegiatan ini anak-anak panti asuhan tersebut dapat mengerti mengenai permasalahan gigi dan mulut yang ada pada dirinya, sehingga dapat memotivasi keinginan untuk melakukan pemeriksaan dan tindakan lebih lanjut ke dokter gigi.
SCHOOL DENTAL HEALTH PROGRAM: TRAINING OF TRAINER KESEHATAN GIGI DAN MULUT PADA GURU DAN ORANG TUA SEBAGAI UPAYA MENUJU PROGRAM “ INDONESIA BEBAS KARIES 2030” Rahman, Kurnia Hayati; paramita, Ayulistya; Sucahyo, Bambang; Revianti, Syamsulina; Pinasti, Restika Anindya; Paramita, Anisa Listya; Prameswari, Noengki; Lelyna, Nora; Handayani, Budi; Widaningsih, Widaningsih; Wedarti, Yoifah Rizka; Nilawati, Nina; Harnowo, Setyo; Prabowo, Puguh Bayu; Damaiyanti, Dian Widya
MAJU : Indonesian Journal of Community Empowerment Vol. 1 No. 6 (2024): MAJU : Indonesian Journal of Community Empowerment, November 2024
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/aj2jgc13

Abstract

Oral and dental health among school-age children is often neglected, despite its importance in supporting their quality of life. A Training of Trainer (ToT) program on oral and dental health was conducted for teachers and parents at MI KH Ramly Surabaya to support the vision of Indonesia Free from Dental Caries 2030. This training included educational sessions and guidance on proper tooth-brushing habits using fluoride toothpaste. The results showed an improvement in participants' knowledge, with higher average post-test scores compared to pre-test scores. Additionally, there was a positive behavioral change among children in maintaining oral hygiene. Although statistical tests indicated insignificant results, the intervention had a practical impact on establishing healthy habits. Support from teachers, parents, and healthcare professionals is essential to reducing the prevalence of dental caries in the future. .
TRAINING OF TRAINER (TOT) DALAM UPAYA PEMELIHARAAN KESEHATAN GIGI UNTUK ORANG TUA DAN SISWA DI SD RADEN PAKU SURABAYA Rahmitasari, Fitria; Rosita Irmawati, Ari; Revianti, Syamsulina; Nilawati, Nina; Widyastuti; Kurniawan, Hansen; Bayu Prabowo, Puguh; Pangabdian, Fani; Nur Ashrin, Meinar; Widaningsih; Handayani, Budi; Khoironi, Emy; Lukisari, Cane; Samadi, Karlina; Harnowo, Setyo
Jurnal Pengabdian Masyarakat Pesisir VOLUME 3 NOMOR 2
Publisher : Universitas Hang Tuah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpmp.v3i2.120

Abstract

Angka kejadian permasalahan kesehatan gigi dan mulut di Indonesia mencapai 57,6%, dimana tergolong sangat tinggi, dengan 93% di antaranya dialami oleh anak-anak usia sekolah. Pengetahuan, sikap, dan perilaku individu memiliki peran yang penting dalam menjaga kesehatan gigi mulut. Lingkungan sekitar anak, terutama keluarga dan guru, memiliki peran yang krusial dalam membentuk sikap dan perilaku mereka. School Health Program (SHP) yang melibatkan pelatihan Training of Trainer (ToT) mengenai kesehatan gigi mulut bagi guru dan orang tua mencakup kegiatan menyikat gigi dua kali sehari selama 21 hari. Program ini bertujuan untuk memberikan pendidikan kepada anak dan orang tua beserta guru tentang perlunya menjaga oral hygiene yang baik serta mengajarkan teknik menggosok gigi dengan pasta gigi berfluoride. Inisiatif ini juga mendukung pencapaian target "Indonesia Bebas Karies 2030" dan dilaksanakan secara daring melalui aplikasi Zoom. Pemahaman peserta terkait kesehatan gigi dievaluasi melalui kuesioner sebelum dan sesudah diberikan pelatihan. Analisis statistik menggunakan uji t-sampel berpasangan untuk mengetahui dan membandingkan nilai rerata sebelum dan sesudah diberikan pelatihan menunjukkan nilai p<0,05, yang mengindikasikan adanya peningkatan pengetahuan yang signifikan mengenai pemeliharaan kesehatan gigi mulut pada guru serta orang tua siswa di SD Raden Paku melalui program ToT. Kegiatan ini terbukti efektif dalam meningkatkan pengetahuan dan perilaku mengenai kesehatan gigi dan mulut setelah materi diberikan. 
EFFORTS TO IMPROVE DENTAL AND ORAL HEALTH KNOWLEDGE IN THE ELDERLY AT UPTD GRIYA WERDHA JAMBANGAN SURABAYA Pinasti, Restika Anindya; Fauziah, Yessy Andriani; Fauzia, Bunga; Holanda, Ghita Hadi; Maharani, Aulia Dwi; Lelyana, Nora; Parisihni, Kristanti; Revianti, Syamsulina; Brahmanta, Arya
Abdi Dosen : Jurnal Pengabdian Pada Masyarakat Vol. 6 No. 4 (2022): DESEMBER
Publisher : LPPM Univ. Ibn Khaldun Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/abdidos.v6i4.1330

Abstract

The aging process is a process of slowly disappearing the ability of tissues to repair themselves or replace and maintain their normal functions. Tooth decay, tooth mobility, caries, halitosis, gingivitis, gingival recession, loss of periodontal attachment, and alveolar bone are changes in periodontal tissue that are commonly found in the elderly. If this is not treated, it can cause mobility and loss of teeth which will interfere with the function and activity of the oral cavity and will have an impact on the quality of life. Dental and oral health problems become one of the problems that have negative implications for overall health that in the end, it affects the quality of life in the elderly. Based on this analysis, in implementing the Tridarma of Higher Education, especially community service, the strategy is to increase public access to dental health services. One form of community service that can be carried out is promotive and preventive efforts. This activity is an educational approach to increase the ability and participation of the elderly community in maintaining dental health which can affect a person's quality of life.
NATIONAL DENTAL HEALTH MONTH PROGRAM 2022 SCHOOL HEALTH PROGRAM WITH ITS KINDERGARTEN Revianti, Syamsulina; Brahmanta, Arya; Agnita Cevanti, Twi; Puspita, Sinta; Khoironi, Emy; Pangabdian, Fani; Widaningsih, Widaningsih; Hanum, Faizah; Listya Paramita, Annisa; Dwi Maharani, Aulia; Paramita, Ayulistya; Iman, Dimas; Rizal, Mohammad Basroni; Anindya Pinasti, Restika; Soesilo, Diana
Abdi Dosen : Jurnal Pengabdian Pada Masyarakat Vol. 7 No. 3 (2023): SEPTEMBER
Publisher : LPPM Univ. Ibn Khaldun Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/abdidos.v7i3.1835

Abstract

Dental and oral health in Indonesia is still a health problem that needs attention. The most concerning thing is that as many as 93% of school-age children experience dental and mouth problems. This can be caused by a lack of knowledge and awareness of dental hygiene. One of the efforts made to increase knowledge and awareness of dental hygiene is by holding an educational activity called the "School Health Program". This activity was carried out in several schools, one of which was TK ITS. This activity began with the Training of Trainer Program given by dentists to teacher cadres and parents of students/students/students about the benefits of good oral hygiene and how to brush your teeth twice a day with fluoride toothpaste. Dentists provide educational materials using power point media and educational videos through the zoom application. There were 5 cadres consisting of 2 teachers and 3 guardians of students who attended this activity. After that, the teacher cadres and parents of the students/students disseminate information to other parents of students/students so that they can become mentors to their children to apply good and correct behavior of brushing their teeth twice a day for 21 days with the method recorded on the given calendar. The hope is that after this program ends, students at ITS Kindergarten have the behavior of maintaining oral hygiene by brushing their teeth twice a day with fluoride toothpaste.
Co-Authors Agnita Cevanti, Twi Aisah Faiz Bachmid Andriani, Dwi Anindya Pinasti, Restika Ariestania, Vivin Artsiana, Sweeta Arya Brahmanta Arya Brahmanta, Arya Aulia Dwi Maharani Bayu Prabowo, Puguh Bunga Fauzia Cane Lukisari, Cane Damaiyanti, Dian Widya Darojati, Rafika Rusydia Diana Soesilo, Diana Dimas Iman Dwi Andriani, Dwi Dwi Maharani, Aulia Dwi Setianingtyas Enggardini, Ajeng Saghita Fani Pangabdian, Fani Fauzia, Bunga Fitria Rahmitasari Fitriano, Yufita Halim, Peggie Octavia Handayani, Budi Hansen Kurniawan Hanum, Faizah Harnowo, Setyo Hartono, Fransiska amelia Hermanto, Eddy Holanda, Ghita Hadi Iman, Dimas Irmawati, Ari Rosita Karlina Samadi, Karlina Karsini, Isidora Karsini, Isidora Khairina, Ajeng Hanun Winny Khoironi, Emy Kristanti Parisihni Kurniawan, Hansen Lelyana, Nora Lelyna, Nora Lestari, Oka Listya Paramita, Annisa Nafi?ah, Nafi?ah Nafiah Nafiah Nafiah, Nafiah Nilawati, Nina Noengki Prameswari Nora Lelyana Nur Ashrin, Meinar Oka Lestari Paramita, Anisa Listya Paramita, Ayulistya Pargaputri, Agni Febrina Pinasti, Restika Anindya Prabowo, Puguh Bayu Prameswari, Noengki Puguh Bayu Prabowo, Puguh Bayu Puspita, Sinta Rahman, Kurnia Hayati Retno Pudji Rahayu Rizal, Mohammad Basroni Rizka, Yoifah Rosita Irmawati, Ari Santoso, Rasmi Putri sari, Rima parwati Shintya Rizki Ayu Agitha Shintya Rizki Ayu Agitha, Shintya Rizki Ayu Sucahyo, Bambang Sutarto, Ayulistya Paramita Syahdinda, Meralda Rossy Tsurayya, Nur Wardana, Enjel Arjuna Susru Wedarti, Yoifah Rizka Wedarti, Yoifah Rizka Wendi, Yoannita Widaningsih Widaningsih Widaningsih, Widaningsih Widyasri Prananingrum WIDYASTUTI Yessy Andriani Fauziah Yunanda, Auliasari