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The effect of spirulina gel on fibroblast cell number after wound healing Fitria Rahmitasari; Wisnu Setyari J; Ester Arijani Rachmat
Dental Journal (Majalah Kedokteran Gigi) Vol. 44 No. 4 (2011): December 2011
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (642.701 KB) | DOI: 10.20473/j.djmkg.v44.i4.p192-195

Abstract

Background: Wound healing treatment after tooth extraction should be an important consideration due to mouth discomfort and pain. Spirulina (blue green algae) consists of C-phycocyanin, b–carotenoids, vitamin E, zinc, some other trace elements and natural phytochemical which are believed to act as antioxidant and takes part in wound healing process. Purpose: The purpose of this study was to examine the effect of spirulina gel on fibroblast cell number after wound healing process. Methods: Twenty eight males guinea pig are devided into four group, 7 guinea pig each. They are control group and treatment group which is given 0%, 3%, 6%, and 12% spirulina gel. After tooth extraction, histopathological evaluation was done to count fibroblast cell. The data was analyzed by One-Way ANOVA and Tukey HSD. Results: The research has proven the relation between the increased growth of fibroblast cell and spirulina gel application. The higher the doses, the more cell growth. Hence, there has been significant different (p < 0.05) among groups. Conclusion: Spirulina gel increases the number of fibroblast in wound after tooth extraction and 12% spirulina gel has the most potential ability.Latar Belakang: Proses penyembuhan luka pasca pencabutan gigi merupakan salah satu hal yang penting karena akan menimbulkan rasa nyeri dan tidak nyaman dalam rongga mulut. Spirulina (Blue green Algae) mengandung C-phycocyanin, b-carotenoids, vitamin E, seng, beberapa trace elemen lainnya, dan phytochemical alami yang terbukti dapat berperan sebagai antioksidan dalam proses penyembuhan luka. Tujuan: Tujuan dari penelitian ini adalah untuk mengetahui efek pemberian gel spirulina terhadap jumlah sel fibroblas pada proses penyembuhan luka pasca pencabutan gigi. Metode: Dua puluh delapan ekor guinea pig jantan dibagi dalam 7 kelompok, masing-masing terdiri dari 4 ekor. Kelompok tersebut adalah kelompok kontrol dan kelompok perlakuan yang diberikan gel spirulina dengan konsentrasi 0%, 3%, 6%, dan 12%. Setelah pencabutan gigi, dilakukan penghitungan sel fibroblas dengan metode histopatologi. Hasil: Penelitian ini menunjukkan adanya hubungan antara pemberian gel spirulina terhadap peningkatan jumlah sel fibroblas. Semakin tinggi dosis gel spirulina akan semakin meningkatkan jumlah sel fibroblas pula. Didapatkan perbedaan yang signifikan antar kelompok (p < 0,05). Kesimpulan: Gel spirulina meningkatkan jumlah sel fibroblas pada luka bekas pencabutan dan gel spirulina dengan konsentrasi 12% mempunyai kemampuan yang paling potensial.
The potential of chitosan combined with chicken shank collagen as scaffold on bone defect regeneration process in Rattus norvegicus Fitria Rahmitasari; Retno Pudji Rahayu; Elly Munadziroh
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 1 (2016): March 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.695 KB) | DOI: 10.20473/j.djmkg.v49.i1.p22-26

Abstract

Background: In the field of dentistry, alveolar bone damage can be caused by periodontal disease, traumatic injury due to tooth extraction, cyst enucleation, and tumor surgery. One of the ways to regenerate the bone defect is using graft scaffold. Thus, combination of chitosan and collagen can stimulate osteogenesis. Purpose: The aim of this study was to examine the potential of chitosan combined with chicken shank collagen on bone defect regeneration process. Method: Twelve Rattus norvegicus were prepared as animal models in this research. A bone defect was intentionally created at both of the right and left femoral bones of the models. Next, 24 samples were divided into four groups, namely Group 1 using chitosan – collagen scaffold (50:50), Group 2 using chitosan collagen-scaffold (80:20), Group 3 using chitosan scaffold only, and Control Group using 3% CMC-Na. On 14th day, those animals were sacrificed, and histopathological anatomy examination was conducted to observe osteoclast cells. In addition, immunohistochemistry examination was also performed to observe RANKL expressions. Result: There was a significant difference in RANKL expressions among the groups, except between Group 3 using chitosan scaffold only and control group (p value > 0.05). The highest expression of RANKL was found in Group 1 with chitosan – collagen scaffold (50:50), followed by Group 2 with chitosan-collagen scaffold (80:20). Moreover, there was also a significant difference in osteoclast generation, except between Group 1 using chitosan – collagen scaffold (50:50) and Group 2 using chitosan-collagen scaffold (80:20), p value < 0.05; and between Group 3 using chitosan scaffold only and control group, p value > 0.05. Less osteoclast was found in the groups using chitosan – collagen scaffold (Group 1 and Group 2). Conclusion: Combination of chitosan and chicken shank collagen scaffold can improve regeneration process of bone defect in Rattus novergicus animals through increasing of RANKL expressions, and decreasing of osteoclast.
Penggunaan Scaffold Kitosan-Aloe Vera Terhadap Proliferasi Sel Fibroblas pada Penyembuhan Luka Pasca Pencabutan Gigi Cavia Cobaya Sularsih Sularsih; Fitria Rahmitasari
Jurnal Material Kedokteran Gigi Vol 7 No 2 (2018): JMKG Vol 7 No 2 September 2018
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (583.702 KB) | DOI: 10.32793/jmkg.v7i2.370

Abstract

Aloe vera has been investigated potential use in tissue engineering. Its bioactive compounds interact with growth factor receptor on the fibroblast and stimulate cells proliferation. The aim of this study was to investigate the proliferation of fibroblast cells on wound healing process of Cavia cobaya dental extraction using scaffold combination chitosan and Aloe vera. Cavia cobaya male with 300-350 grams of weight and in the age of 3 to 3.5 months. It divided into ten groups (n=5). For Treatment groups, Aloe vera scaffold(Av); Chitosan Scaffold (Ch); Scaffold combination chitosan-Aloe vera (Ch-Av) and scaffold combination chitosan- Aloe vera- hidroksiapatit(Ch-Av-HA) were applied into the socket of dental extraction. For control group, which were not given scaffold. Cavia cobaya were decaputated at 7 and 14 days and the jaw in the treated regions and control group were cut for Histopatology Anatomy examination to investigate the proliferation of fibroblast cells. Data were analyzed using One way Anova test. The proliferation of fibroblast cells were found higher in the group which given Av-Ch and Av-Ch-HA scaffold. The result showed significant differences in proliferation of fibroblast cells for 7 and 14 days observation compared to control group (p<0,05). The application of scaffold combination Chitosan and Aloe vera accelerate the wound healing process of Cavia cobaya dental extraction.
Agen Crosslink dalam Pembuatan Biomimetic Scaffold Sebagai Material Rekayasa Jaringan Tulang Fitria Rahmitasari; Sularsih Sularsih
Jurnal Material Kedokteran Gigi Vol 8 No 2 (2019): JMKG Vol 8 No 2 September 2019
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jmkg.v8i2.344

Abstract

Case of bone destruction such as socket of post-tooth extraction and periodontitis can cause pain and disrupt the function of mastication, so it takes a tissue engineering material in the form of biomimetic scaffold by mimicking the structure and the original function of bone. Material for bone tissue engineering that has grown widely include the use of collagen, gelatin, chitosan, and hydroxyapatite. These materials can be mutually combined using crosslink agents in the scaffold fabrication process. Determine the role of crosslink agents in the manufacture of biomimetic scaffolds as bone tissue engineering materials in the field of dentistry. Material in the scaffold fabrication is a polymer which can be modified by the incorporation of other polymers. Additionally, polysaccharides have abundant functional groups such as hydroxyl groups, amino groups and/or carboxylic acid groups, amenable to various types of chemical modifications. Modification with other polymers can be done by adding crosslink agents by irradiation or through chemical reactions. The role of a crosslink agent in a material modification can increase the material resistance to heat, light, other physical properties, and can increase the degree of dimensional stability and mechanical strength so these values are important to consider for the survival of cells in scaffolds at early time points. Different forms of crosslink agents can be utilized to obtain good biomimetic scaffold so that optimize the osteoconductivity properties of bone tissue engineering.
Scaffold 3D Kitosan dan Kolagen Sebagai Graft pada Kasus Kerusakan Tulang Fitria Rahmitasari
Jurnal Material Kedokteran Gigi Vol 5 No 2 (2016): JMKG Vol 5 No 2 September 2016
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jmkg.v5i2.246

Abstract

Kerusakan tulang alveolar dalam kasus kedokteran gigi dapat disebabkan oleh penyakit periodontal, trauma pasca pencabutan gigi, post enukleasi kista, dan post operasi tumor. Kitosan merupakan bahan yang sering digunakan sebagai bahan graft namun penggunaannya sendiri kurang osteokonduktif apabila tidak dikombinasikan dengan bahan lain. Kitosan dapat dikombinasikan dengan kolagen dalam bentuk sediaan scaffold 3D untuk penyembuhan kasus kerusakan tulang. Untuk mengetahui sifat osteokonduktifitas bahan kitosan dan kolagen berupa sediaan scaffold 3D. Scaffold 3D kitosan dan kolagen dapat menirukan struktur dan fungsi alami dari protein ekstraseluler matriks, membentuk struktur unik yang dapat meningkatkan kekuatan mekanik, menurunkan tingkat biodegradasi, dan kombinasi keduanya dapat menghasilkan ion kompleks yang berperan untuk merangsang proliferasi dan diferensiasi sel sehingga dapat mempercepat proses penyembuhan tulang. Scaffold 3D kitosan dan kolagen berperan dalam proses penyembuhan tulang karena kedua bahan tersebut membentuk senyawa ion kompleks yang dapat meningkatkan sifat osteokonduktifitas.
Particle size analysis of yellowfin tuna (Thunnus albacares) skin collagen powder using papain-soluble collagen method with varying NaCl concentrations: an experimental laboratory Fatryana, Destya Arizha; Rahmitasari, Fitria; Nanik K., Chaterina Diyah; Rizal, Moh. Basroni; Winias, Saka
Padjadjaran Journal of Dentistry Vol 36, No 2 (2024): July 2024
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol36no2.54588

Abstract

ABSTRACTIntroduction: The skin of yellowfin tuna (Thunnus albacares) contains high protein, which could potentially be a halal collagen product. Collagen extraction using the papain-soluble collagen method has the advantage of producing a higher collagen yield compared to the acid method. Particle size, one of the physical properties of collagen, plays a crucial role in its efficacy in dentistry. This study aims to analyze the particle size of collagen powder synthesized   from Thunnus albacares skin using the papain-soluble collagen method, with varying concentrations of NaCl. Methods: Type of research is an experimental laboratory. Thunnus albacares skin was synthesized by chopping, cleaning, and soaking in a 0.1 M NaOH solution. The extraction process used the enzyme papain and 0.5 M of acetic acid. Samples were divided into four groups, each with different concentrations of NaCL: 0, no NaCL;, 0.9 M NaCl, 1.3 M NaCl, and 1.7 M NaCl. After centrifugation, the samples were freeze-dried. The particle size of collagen powder was measured using a Particle Size Analyzer test tool. The data collected was then analyzed using the Mann-Whitney test. Results: Particle size distributions are as follows: K group (3.36 nm), P1 (1.842 nm), P2 (3.36 nm), and P3 (10.12 nm). There is a significant difference in groups K-P1 and P3, P1-P2 and P3, and P2-P3 (p<0.05). However, there is no significant difference in groups K-P2 (p>0.05). Conclusion: Particle size of this research produced nano-sized collagen powder, with the lowest particle size observed in the 0.9 M NaCl group, measuring at 1.842 nm. The particle size increased in the group without NaCl and in the 1.3 M NaCl group at 3.36 nm, and reached the highest value in the 1.7 M NaCl group at 10.12 nm. 
Pendidikan Dan Pelatihan Kader Sebagai Salah Satu Pilar Penting Perubahan Perilaku Di Bidang Kesehatan Gigi Dan Mulut Pranoto, Amelia Elizabeth; Aprilia, Aprilia; Rahmitasari, Fitria; Soesilo, Diana; Puspita, Sinta; Riski, Mardiyanti; Ariestania, Vivin; Lestari, IGAM Oka; Raharjo, Pambudi; Wijaya, Yongki Hadinata; Rizal, Mohammad Basroni; Sarianoferni, Sarianoferni; Hendro, Onge Victoria; Wira, Rizko; Sularsih, Sularsih; Cahyo, Benny Dwi; Paramita, Putri
Jurnal Pengabdian Masyarakat Bangsa Vol. 2 No. 11 (2025): Januari
Publisher : Amirul Bangun Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59837/jpmba.v2i11.1930

Abstract

Lima dari sepuluh anak-anak Indonesia usia sekolah dasar memiliki masalah gigi berlubang. Hal ini disebabkan oleh kebersihan rongga mulut yang buruk dan kurangnya pengetahuan anak-anak terkait pentingnya menjaga kesehatan gigi dan mulut. Anak-anak juga seringkali tidak menyikat gigi di waktu yang benar, ditambah lagi cara menyikat gigi yang keliru menjadi salah satu faktor munculnya gigi berlubang. Dokter gigi dari Fakultas Kedokteran Gigi, Universitas Hang Tuah Surabaya melakukan kegiatan pengabdian masyarakat berupa School Health Program – Training of Trainer (SHP-ToT). Kegiatan ini bertujuan untuk menciptakan kader kesehatan gigi dan mulut yang berasal dari lingkungan internal sekolah. Diharapkan kader-kader tersebut dapat menjadi pilar penggerak utama pada pendidikan anak-anak usia sekolah dasar akan pentingnya menjaga kebersihan gigi dan mulut secara berkelanjutan. Kader akan diberi pelatihan dan diuji sejauh mana pemahamannya terkait kesehatan gigi dan mulut. Setelah pelatihan selesai, kader akan terus dipandu untuk mengajarkan anak-anak sekolah dasar tentang pentingnya menjaga kesehatan rongga mulut sekaligus menjadi supervisor anak-anak agar mau menyikat gigi pagi dan malam selama 21 hari berturut-turut.
INDEKS DMF-T dan def-t MURID USIA 7-10 TAHUN MI TARBIYATUT THOLABAH LAMONGAN Kurniawan, Hansen; Fitriani, Yufita; Sari, Rima Parwati; Handayani, Budi; Rahmitasari, Fitria; Prabowo, Puguh Bayu; Aprilia, Aprilia; Ariestania, Vivin; Priambodo, Nur Tsurayya; Saptaswari, Dianty
MAJU : Indonesian Journal of Community Empowerment Vol. 2 No. 1 (2025): MAJU : Indonesian Journal of Community Empowerment, Januari 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/fwwcfg92

Abstract

Background Based on the 2018 Basic Health Research (Riskesdas), it was reported that the prevalence of dental and oral problems in Indonesia is still very high, namely 57.6 percent. Of this figure, only 10.20% have received treatment. Dental and oral health in Indonesia, especially in coastal areas, is still a complex problem. (Komarudin, Apriasari and Azizah, 2021) Lack of public attention and equal distribution of health facilities, especially in the field of Dentistry, are still factors that influence public understanding of dental and oral health. Dental and oral health status can be assessed using the DMF-T and def-t indices. Objective To assess the dental and oral health status in children aged 7-10 years MI Tarbiyatut Tholabah, Paciran Lamongan Method Dental and oral examination of children aged 7-10 years MI by assessing the DMF-T and def-t indices. Conclusion The results of the dental and oral examination of students at MI Tarbiyatut Tholabah, Paciran Lamongan with a def-t value of 2.71 which means the dental and oral health status with moderate results, and the DMF-T index with a result of 0.56 which means very low.
Characteristics of chitosan from Penaeus monodon on chitosan-gelatin suspension viscosity Prananingrum, Widyasri; Setiawan, Grace Caroline; Putri, Vindya Meinda; Wahyuning, Baiq Nadia Risna; Rahmitasari, Fitria; Sularsih, Sularsih; Ramadhania, Nurina Rizka; Fatmawati, Sri
Dental Journal (Majalah Kedokteran Gigi) Vol. 58 No. 2 (2025): June
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v58.i2.p135-143

Abstract

Background: Chitosan synthesized from Penaeus monodon shells was developed into a chitosan-gelatin suspension as an injectable bone substitute for socket preservation. Purpose: To investigate the characteristics of chitosan from P. monodon shells and their influence on the viscosity of a chitosan-gelatin suspension. Methods: P. monodon shells from Tarakan Waters were prepared using three methods: Group 1) deproteinization-depigmentation-deacetylation, Group 2) demineralization-depigmentation-deacetylation, and Group 3) deproteinization-demineralization-depigmentation-deacetylation. The chitosan was characterized by morphology, moisture and ash content, molecular weight (MW), deacetylation degree (DD), and viscosity. This chitosan was made into a chitosan-gelatin suspension with a ratio of 45:55 (w/w%) (95 ml:110 ml). The differences in viscosity of the chitosan-gelatin suspension were determined using Kruskal–Wallis and Mann–Whitney tests. The effects of the chitosan’s MW and DD on the viscosity of the chitosan-gelatin suspension were analyzed using Spearman’s correlation. Results: Group 2 had the highest moisture content (10.63%), MW (159.68 kDa), viscosity of the chitosan powder (5.53 dPa.s), and viscosity of the chitosan-gelatin suspension (40.20 cps). Group 1 had the highest ash content (27.83%) and DD (93.72%). Group 3 showed the lowest ash content (1.06%), MW (37.12 kDa), and DD (86.22%), but it had good viscosity of the chitosan-gelatin suspension (37.25 cps). A significant difference in viscosity was found between the chitosan-gelatin suspension groups. Spearman’s correlation coefficients between the viscosity of the chitosan-gelatin suspension and MW and between the viscosity of the chitosan-gelatin suspension and DD were 0.389 and −0.195, respectively. Conclusion: The viscosity of a chitosan-gelatin suspension is influenced by the MW and DD of the chitosan powder. Chitosan from P. monodon can potentially be an injectable bone substitute in socket preservation.
UPAYA PENINGKATAN WAWASAN DALAM MENJAGA KESEHATAN GIGI DAN MULUT PADA GURU DAN ORANG TUA SISWA TK CAHAYA TAZKIA SURABAYA Rahmitasari, Fitria; Lestari, I.G.A.M Oka; Nilawati, Nina; Setianingtyas, Dwi; Yoyada, Novendy; Tantra, Ivan; Indarti, Caecilia; Prabowo, Puguh Bayu; Emilda, Yulie; Hartono, Mardiyanto Riski; Irmawati, Ari Rosita; Ayu A, Shintya Rizki
Jurnal Pengabdian Masyarakat Pesisir VOLUME 3 NOMOR 1
Publisher : Universitas Hang Tuah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpmp.v3i1.96

Abstract

Angka proporsi perilaku menyikat gigi secara benar sebanyak dua kali sehari yaitu setelah sarapan pagi dan sebelum tidur malam hanya sebesar 2,8% pada masyarakat Indonesia. Sejumlah 93% anak-anak usia sekolah mengalami masalah gigi dan mulut, dimana angka ini merupakan angka prevalensi yang sangat tinggi. Upaya Kementerian Kesehatan Republik Indonesia dalam menurunkan angka prevalensi karies gigi yaitu dengan melaksanakan program “Indonesia Bebas Karies 2030”. PT. Unilever Tbk. melakukan kerjasama dengan Asosiasi Fakultas Kedokteran Gigi Indonesia (AFDOKGI), Asosiasi Rumah Sakit Gigi dan Mulut Pendidikan Indonesia (ARSGMPI), Persatuan Dokter Gigi Indonesia (PDGI), dan Fakultas Kedokteran Gigi Universitas Hang Tuah akan melakukan program “School Health Program” (SHP) dalam rangkaian kegiatan Bulan Kesehatan Gigi Nasional (BKGN) 2022. Program Training of Trainer (ToT) pada guru dan orang tua untuk kegiatan sikat gigi pagi dan malam 21 hari mengajarkan anak-anak dan orang dewasa tentang manfaat kebersihan mulut yang baik dan menunjukkan kepada mereka cara menyikat gigi dua kali sehari dengan pasta gigi berfluoride untuk mewujudkan Indonesia bebas karies di tahun 2030. Kegiatan ini mengunakan sistem digital / online melalui aplikasi zoom. Peserta kegiatan ini adalah 5  orang tua dan 5 guru di TK Cahaya Tazkia. Dalam memberikan edukasi, para dokter gigi menggunakan media power point dan video edukasi. Bentuk materi edukasi yang diberikan meliputi video animasi sikat gigi, kalender digital sikat gigi 21 hari, poster/ flyer digital, flipchart. Sebelum memulai edukasi para peserta dilakukan pre test terlebih dahulu tentang pengetahuan seputar gigi dan kebersihannya sesuai materi yang akan disampaikan, setelah diakhir sesi zoom tersebut juga dilakukan post test dengan materi yang sama untuk mengetahui tingkat pengetahuan dan pemahaman peserta terhadap materi tersebut. Kegiatan penyuluhan kesehatan gigi dan mulut melalui ToT pada guru dan orang tua siswa/siswi di TK Cahaya Tazkia Surabaya memberikan hasil peningkatan tingkat pengetahuan guru dan orang tua dalam menjaga kesehatan gigi dan mulut. Hasil analisis statistik menggunakan paired sample t-test untuk membandingkan nilai rata-rata pre test dan post test siswa/siswi menunjukkan nilai signifikansi p=0.001 (p<0.05) yang berarti bahwa terdapat perbedaan yang signifikan pada tingkat pengetahuan guru dan orang tua siswa/siswi di TK Cahaya Tazkia dalam menjaga kesehatan gigi dan mulut sebelum dan sesudah diberi materi Dental Health Education (DHE) melalui “Training of Trainer”.