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Journal : Jurnal Penelitian Pertanian Terapan

Multiplikasi Mata Tunas Pisang ‘Cavendish’ In Vitro Pada Berbagai Konsentrasi Benziladenin Maulida, Desi; Erfa, Lisa; Sesanti, Rizka Novi
Jurnal Penelitian Pertanian Terapan Vol 18, No 1 (2018)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.958 KB) | DOI: 10.25181/jppt.v18i1.748

Abstract

Banana is one of the most important global agriculture commodities. Asia accounts for the production of bananas amounted to 56.4% of total banana in the world and Indonesia is one of the banana-producing countries in Asia. The demand for bananas increased need is anticipated with good cultivation techniques to meet domestic and international market demand. Plant regeneration in vitro tissue culture techniques or can be used to produce propagules true-to-type in large quantities in a relatively short period and free from disease. This research aims to study the influence of various concentrations of BA (0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0 mg/l) against the growth and reproduction of Cavendish banana propagules in vitro. Time appears buds, propagules length and the number of propagules per explant, and the number of primary roots of plants per explant observed after eight weeks of culture. The experiment results show that after explant grown for eight weeks on the media's treatment shows the different response. The increased concentration of BA can increase the number of shoots. The increased concentration of BA from 0.5 mg/l be 1-3 mg/l increased the number of propagules, but the number of propagules will decrease in the concentration of BA more 3.5 or 4 mg/l. In addition to BA concentration 0.5, 1 and 1.5 mg/l MS media into producing shoot buds appear faster time high, highest propagules and root amount more on banana propagules Cavendish, but the number of shoots is fewer. The best of BA concentrations to induce propagules plant bananas Cavendish is 3.0 mg/l.
Pengaruh Pemberian Iba (Indole Butyric Acid) dan Konsentrasi Naa (Naphthalene Acetic Acid) terhadap Keberhasilan Penyetekan Sirih Merah (Piper Crocatum Ruiz and Pav.) Desi Maulida; Rugayah Rugayah; Dewi Andalasari
Jurnal Penelitian Pertanian Terapan Vol 13 No 3 (2013)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.444 KB) | DOI: 10.25181/jppt.v13i3.179

Abstract

The research was conducted at the cutting red betel (Piper crocatum Ruiz and Pav.) to determined the effect of (1) the difference growth of cuttings of red betel which are given IBA and without IBA, (2) the concentration of NAA on growth of cutting red betel, (3) the concentration of NAA on growth of cutting red betel on each given IBA. The treatment was arranged in factorial (2 x 4) in randomized block design with three replication. The first factor were without IBA (a0) and the given of 1.000 ppm IBA (a1). The second factor were the concentration of NAA consists of: 0 ppm (b0), 1.000 ppm (b1), 2.000 ppm (b2), and 4.000 ppm (b3). The results showed that the NAA concentration of 4000 ppm produced the most number of roots on either at the node or at the base of cutting. Planting cutting red betel which given IBA 1.000 ppm was able to accelerated the time leaves open and increased the number of cutting which germinate. The mixtured of giving IBA 1.000 ppm and the concentration of NAA 4.000 ppm, produced the most number of cutting that germinated. Keywords : red betel, given IBA, dan concentration NAA
Keberhasilan Aklimatisasi dan Pembesaran Bibit Kompot Anggrek Bulan (Phalaenopsis) Pada Beberapa Kombinasi Media Tanam Lisa Erfa; Desi Maulida; Rizka Novi Sesanti; Yuriansyah Yuriansyah
Jurnal Penelitian Pertanian Terapan Vol 19 No 2 (2019)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v19i2.1420

Abstract

Aklimatisasi merupakan tahap yang kritis bagi plantlet. Pada lingkungan yang baru plantlet dapat mengalami cekaman lingkungan. Kondisi lingkungan seperti media yang tidak mendukung pada tahap aklimatisasi dapat menyebabkan kematian plantlet.  Penelitian ini bertujuan:  1)  Mendapatkan kombinasi  media yang paling baik bagi keberhasilan aklimatisasi plantlet anggrek Phalaenopsis; 2)Mengetahui apakah terdapat interaksi antara media bagian bawah dengan atas pot; 3) Mendapatkan kombinasi media yang paling baik bagi pertumbuhan bibit anggrek anggrek Phalaenopsis pada tahap pembesaran, dan 4) Melihat apakah media sabut kelapa dapat digunakan untuk aklimatisasi dan pembesaran anggrek Phalaenopsis.  Penelitian dilaksanakan di rumah jaring Politeknik Negeri Lampung dari bulan Maret hingga Agustus 2018.  Plantlet diaklimatisasikan 4 minggu.  Percobaan faktorial dilaksanakan dalam rancangan acak kelompok. Faktor A :  macam media bagian bawah (bata/arang).  Faktor B :  macam media bagian atas (moss sphagnum/pakis/sabut kelapa).  Enam kombinasi perlakuan dicobakan (masing-masing dengan 10 ulangan), yaitu:   P1 (bata+moss); P2 (bata+pakis); P3 (bata+sabut kelapa); P4 (arang+moss); P5 (arang+pakis); dan P6 (arang+sabut kelapa).  Peubah yang diamati: 1)  persen keberhasilan plantlet menjadi bibit, 2) tinggi bibit, 3) panjang daun, 4) lebar daun, dan 5) jumlah daun.   Data yang diperoleh dilakukan analisis ragam dan dilanjutkan dengan uji BNJ 5%.  Hasil penelitian menunjukkan: 1) Media moss atau pakis dikombinasikan dengan bata maupun arang memberikan persen keberhasilan aklimatisasi plantlet anggrek Phalaenopsis yang paling baik (100%); 2) Tidak terdapat interaksi antara media bagian atas dengan bagian bawah; 3) Kombinasi media yang paling baik bagi pertumbuhan bibit anggrek Phalaenopsis pada tahap pembesaran adalah moss baik dikombinasikan dengan bata ataupun arang, kemudian diikuti oleh pakis (dikombinasikan dengan bata ataupun arang); dan 4) Media sabut kelapa dapat digunakan untuk aklimatisasi dan pembesaran bibit anggrek Phalaenopsis bila  dilakukan penyiraman yang lebih intensif.
Kultur Embrio Kelapa Kopyor Menggunakan Beberapa Konsentrasi BA Dan Air Kelapa Desi Maulida; Lisa Erfa; Marveldani
Jurnal Penelitian Pertanian Terapan Vol 20 No 3 (2020)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v20i3.1929

Abstract

Coconut Kopyor is an abnormal fruit, the ordinary coconut flesh is attached to the shell and separated from coconut water while Coconut Kopyor, fruit flesh is not attached to the shell but is mixed with coconut water. Kopyor coconut cannot be used as a seed (seed). The growth of kopyor coconut embryos can only be done in a laboratory with embryo culture technology. Embryo culture is the only way to produce true to type coconut kopyor seeds that can produce 80% kopyor coconut in one kopyor coconut plant, kopyor coconut seedlings produced can support government programs in increasing the productivity of kopyor coconut through the provision of quality seeds. Research on kopyor coconut embryo culture was carried out at the Laboratory of Tissue Culture at Lampung State Polytechnic. The experiment was carried out using a RAL, with 15 treatment combinations of BA media formulations (0, 2, and 4) with coconut water (0 ml / l, 100, 150, 200, 250 ml / l). The observed variables were the percentage of embryos sprouting, when the shoot appeared, shoot height, number of roots, and number of leaves. The results showed that the use of BA 4 mg / l without the addition of coconut water increased the percentage of sprouts, faster time for shoots, and highest shoot height.