Claim Missing Document
Check
Articles

Antioxidant Activity of n-Hexane Subfraction of Buas-Buas Leaf (Premna serratifolia L.) by DPPH Method Darin Iftinan; Isnindar Isnindar; Sri Luliana
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.569-573

Abstract

Buas-buas (Premna serratifolia L.) is a plant traditionally used as medicine and widely grown in West Kalimantan with various species. There are many benefits of buas-buas plants as medicine, one of which is as an antioxidant. The purpose of this study was to determine the percent inhibition value that describes the antioxidant activity of ethanol extract, n-hexane fraction, and n-hexane subfraction of buas-buas leaves. The n-hexane fraction was obtained by liquid-liquid extraction with separatory funnel from ethanol extract and the fraction was isolated by column chromatography. The antioxidant activity of the ethanol extract, n-hexane fraction, and subfractions C1, C2, C3, D123, and E3F123 was assessed using DPPH (2,2-Diphenyl-1-Picrylhydrazyl). The results showed that ethanol extract, n-hexane fraction, and subfractions C1, C2, C3, D123, and E3F123, had antioxidant activity with percent inhibition values of 57.946; 53.078; 52.652; 48.706; 49.745; 50.490; and 50.639%, respectively, which indicated that each sample had good antioxidant activity, except for subfractions C2 and C3.
Effect of maceration and mae (microwave assisted extraction) on antioxidant activity of methanol Extract of simpur air leaves (dillenia suffruticosa (griff.) martelli) Isnindar Isnindar; Siti Nurhayati; Sri Luliana; Meri Ropiqa
Jurnal Teknosains Vol 15, No 1 (2025): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.106535

Abstract

The simpur air plant (Dillenia suffruticosa) is one of the plants used in traditional medicine to treat various diseases and has potential as an antioxidant. Antioxidants are compounds that inhibit, prevent, and reduce oxidative damage to target molecules. The effectiveness of extracting active compounds depends on the extraction method used. Maceration requires a relatively long duration, so faster and more efficient alternatives, such as MAE, are needed. However, heat-sensitive compounds may degrade if the power is too high or the extraction time is prolonged. Therefore, a comparison between maceration and MAE methods is necessary. This study aims to determine the effect of maceration and MAE on the antioxidant activity of the methanol extract from the simpur air leaves. Samples were then analyzed using TLC with a mobile phase of toluene, ethyl acetate, and formic acid (3:5:1 v/v/v). Antioxidant activity was assessed using the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, and data were analyzed with SPSS. TLC results confirmed the presence of flavonoids indicated by a yellow color after spraying with AlCl3, with IC50 values supporting their antioxidant potential. The Rf values of extracts obtained by maceration and MAE with the rutin standard (0.22) and quercetin standard (0.8). The MAE method produced higher antioxidant activity than maceration, with IC₅₀ values of 5.942 ± 0.345 μg/mL and 10.498 ± 0.213 μg/mL, respectively, both classified as strong antioxidants (IC50 < 50). Independent sample t-test analysis yielded a p-value of less than 0.05, indicating a significant difference between the two methods. The methanol extract of simpur air leaves extracted by the MAE method produces better antioxidant activity than the maceration method.