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All Journal ACTA VETERINARIA INDONESIANA ARSHI Veterinary Letters Veterinary Biomedical and Clinical Journal Jurnal Veteriner dan Biomedis Current Biomedicine Genbinesia Journal of Biology
Amaq Fadholly
Division Of Pharmacology And Toxicology, School Of Veterinary Medicine And Biomedical Sciences, IPB University, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Impact of Spirulina platensis Extract as an Immunostimulant on the Histopathological Intestinal of Tilapia Fish (Oreochromis niloticus) Infected with Aeromonas salmonicida Ramadhan, Maharani Kartika; Kurniasih; Untari, Tri; Fadholly, Amaq
Jurnal Veteriner dan Biomedis Vol. 3 No. 2 (2025): September
Publisher : Sekolah Kedokteran Hewan dan Biomedis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jvetbiomed.3.2.%p.

Abstract

Furunculosis in fish results in financial detriment to freshwater aquaculture in Indonesia. Furunculosis, an infectious disease produced by Aeromonas salmonicida, presents a considerable risk to the large-scale farming of both salmonid and non-salmonid species in freshwater environments. This study sought to evaluate the impact of Spirulina platensis extract on the histopatological intestinal health of tilapia infected with Aeromonas salmonicida. Thirty tilapia, each around 10 cm in length, were distributed into five groups. Negative control (P0-) without immersion in Spirulina platensis extract and free from Aeromonas salmonicida infection. Positive control (P0 +) not immersed in Spirulina platensis extract, infected with Aeromonas salmonicida. Tilapia subjected to doses of 400 mg/L (P1), 600 mg/L (P2), and 800 mg/L (P3) The extraction of Spirulina platensis occurred for three hours on the seventh day (first immersion) and the fourteenth day (second immersion), followed by an evaluation of Aeromonas salmonicida infection on the twenty-first day. A necropsy of the fish was performed on day 28. Histopathological analysis revealed that Spirulina platensis extract reduced intestinal villous erosion and inflammatory cell density in the intestines of tilapia. Immunohistochemical staining demonstrated a decrease in the presence of Aeromonas salmonicida within intestinal tissue at a dosage of 800 mg/L. This work indicates that more research is necessary to examine the histological features of the intestines of tilapia infected with Aeromonas salmonicida with those supplemented with Spirulina platensis through feed.
Cytotoxicity of Gambir Leaf Infusion (Uncaria gambir Roxb.) on T47D Cells: In Vitro Assay Fadholly, Amaq
Jurnal Veteriner dan Biomedis Vol. 3 No. 2 (2025): September
Publisher : Sekolah Kedokteran Hewan dan Biomedis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jvetbiomed.3.2.%p.

Abstract

Cancer is a cellular pathology marked by the disruption of regulatory mechanisms governing the cell cycle and homeostasis in multicellular organisms. This study seeks to evaluate the cytotoxic effects and IC50 value of Uncina gambir leaf infusion on the T47D cell line, utilized as a model for mammary cancer. The cytotoxic activity of Uncina gambir leaf extract  was assessed by delivering five concentrations of the test substance: 10 μg/ml, 20 μg/ml, 40 μg/ml, and 80 μg/ml to T47D cell lines, followed by a 24-hour incubation period. Cell counting was conducted following the administration of MTT and SDS stop solution. The inhibition percentages for each test sample concentration were 25.11%, 33.76%, 43.79%, and 48.09%, respectively. The extract of Uncina gambir leaf infusion exhibits an IC50 value of 502.12 μg/mL. The IC50 value of Uncina gambir leaf infusion against T47D cell lines acts as non toxic for the cytotoxicity group. Consequently, it may be inferred that Uncina gambir leaf extract exhibits no cytotoxicity towards T47D cell lines.
Efikasi Infusa Daun Sawo Duren (Chrysophyllum Cainito L.) Sebagai Antidiare Pada Mencit (Mus Musculus) Istiqmal, Kahfi Aulia; Agungpriyono, Srihadi; Fadholly, Amaq
Jurnal Veteriner dan Biomedis Vol. 3 No. 2 (2025): September
Publisher : Sekolah Kedokteran Hewan dan Biomedis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jvetbiomed.3.2.%p.

Abstract

Daun sawo duren (Chrysophyllum cainito L.) atau kenitu mengandung senyawa berupa alkaloid, fenol, sterol, triterpen dan flavonoid. Senyawa tersebut memiliki potensi sebagai sediaan antidiare. Penelitian ini bertujuan mengetahui efikasi antidiare infusa daun sawo duren dengan menggunakan metode proteksi intestinal dan mengetahui kandungan metabolit sekunder daun sawo duren menggunakan pemeriksaan fitokimia. Penelitian ini juga mencari konsentrasi sedian infusa daun sawo duren yang paling efektif sebagai antidiare pada mencit. Mencit jantan sebanyak 30 ekor dikelompokkan menjadi tiga kelompok yang terdiri atas dua kelompok kontrol yaitu kontrol positif berupa suspensi loperamide HCl dan kontrol negatif menggunakan Oleum ricini. Tiga kelompok perlakuan lainnya menggunakan infusa dengan konsentrasi bertingkat (25%, 50%, 100%). Data hasil penelitian antidiare diolah menggunakan aplikasi minitab-18 dengan metode uji one way ANOVA dan uji tukey. Hasil penelitian menunjukkan infusa daun sawo duren dengan konsentrasi 25%, 50%, dan 100% memiliki aktivitas antidiare.
Uji Toksisitas Akut Ekstrak Daun Ketepeng Cina (Cassia alata) pada Tikus (Rattus norvegicus) Ramadhan, Maharani Kartika; Fadholly, Amaq
Acta VETERINARIA Indonesiana Vol. 13 No. 2 (2025): Juli 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.2.%p

Abstract

The leaves of Cassia alata exhibit potential properties as antioxidants, immunostimulants, antimicrobials, anti-dengue agents, and laxatives. This study aimed to determine the LD50 value of the administration of Cassia alata leaves extract using the Thompson-Weil method. A total of 25 rats (Rattus norvegicus) were utilized in the study, divided into five dose groups. One group served as the control (P0), receiving only distilled water, while the four treatment groups were administered doses of 500 (P1), 1000 (P2), 1500 (P3), and 2000 (P4) mg/kg BW. Toxic symptoms and changes in body weight were observed for 14 days. The results showed that there was one death at a dose of 1500 mg/kg BW and two deaths at a dose of 2000 mg/kg BW. The administration of Cassia alata leaf extract resulted in toxic symptoms, including reduced motor activity, respiratory difficulties, and weight loss. The LD50 value obtained was 1.9 g/kg BW. The extract of Cassia alata leaves exhibit a toxic effect on rats.
A bioinformatics approach to design a novel epitope-based vaccine against Simian Immunodeficiency Virus (Retroviridae: Lentivirus) Dhea Kharisma, Viol; Ansori, Arif Nur Muhammad; Ullah, Md. Emdad; Dings, Tim Godefridus Antonius; Probojati, Rasyadan Taufiq; Fadholly, Amaq; Turista, Dora Dayu Rahma; Tacharina, Martia Rani; Zainul, Rahadian
Genbinesia Journal of Biology Vol. 2 No. 1 (2022): November 2022
Publisher : Generasi Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55655/genbinesia.v2i1.26

Abstract

Simian Immunodeficiency Viruses (SIV) have been found to naturally infect African nonhuman primates (NHP). This causative agents are important and one of the special interest as the root cause of the HIV/AIDS pandemic, one of the most threatening infectious diseases worldwide. The aim of this study was to design an epitope-based vaccine using bioinformatics approaches of the circulating SIV in Kenya, Africa. In this study, we used 17 partial SIV envelope glycoprotein genes retrieved from GenBank® (National Center for Biotechnology Information, USA). We analysed the candidate epitopes using the Immune Epitope Database and Analysis Resource. Then, we performed the protective antigens prediction usingVaxiJen. Interestingly, this study revealed the data of B cell epitope prediction, protective antigens prediction, and molecular phylogenetic of circulating SIV in Kenya, Africa. In sum, this study can be used to design a novel epitope-based vaccine against SIV. We suggest further studies to conduct confirmatory experiments (in vitro and in vivo).
Co-Authors Adi Sofyan Ansori, Muhammad Andriyanto Andriyanto Anggraeni, Henny Endah Anisa Rahma Aulia Andi Mustika Aurellia, Sharon Dhea Kharisma, Viol Diah Nugrahani Pristihadi Dings, Tim Godefridus Antonius Dwi Budiono Fedik Abdul Rantam Firdaus, Munira Laeli Hanum, Nur Aisah Hendri Zonathan Heryudianto Vibowo Iryanto, Anetonia Felicia Istiqmal, Kahfi Aulia Ivan Taufiq Nugraha Kukuh Amru Dhiya Rahmat Kurniasih Lina Noviyanti Sutardi Pradnya Calista Purohita, Adwisto Saktika Putri, Imelya Andira Putri, Ramadita Aisyah Rahadian Zainul Ramadhan, Maharani Kartika Rasyadan Taufiq Probojati Sajidah, Halilah Wafa’ Saragih, Mariah Mada Rahma Nauli Srihadi Agungpriyono Tacharina, Martia Rani Tekad Urip Pambudi Sujarnoko Tri Untari Turista, Dora Dayu Rahma Ullah, Md. Emdad