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All Journal KOMUNITAS: INTERNATIONAL JOURNAL OF INDONESIAN SOCIETY AND CULTURE Local Wisdom : Jurnal Ilmiah Kajian Kearifan Lokal Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Abdi Insani BIOEDUPAT: Pattimura Journal of Biology and Learning Pakem : Jurnal Pengabdian Kepada Masyarakat Rumphius Pattimura Biological Journal Kalwedo Sains (KASA) Berita Biologi
Ferymon Mahulette, Ferymon
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EFFECTIVENESS OF ANTISEPTIC BASED ON GANDARIA LEAVES (Bouea macrophylla Griff) ON THE GROWTH OF Staphylococcus aureus AND Escherichia coli Lelapary, Claudia; Pattipeilohy, Mery; Mahulette, Ferymon
RUMPHIUS Vol 4 No 2 (2022): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv4i2p047-051

Abstract

Gandaria leaves (Boea macrophylla Griff) have potential as antibacterial. The ability to inhibit bacterial growth is inseparable from the role of active compounds, namely flavonoids, tannins, quinones and triterpenoids. Growth by microbes needs to be controlled, namely by inhibiting microorganisms, one way is by using chemicals, namely antiseptics. This study aims to determine the effectiveness of antiseptics made from gandaria leaves against the growth of S. aureus and E. coli bacteria. The concentrations of the ethanol extract of gandaria leaves used were 5%, 15% and 25% and betadine (+) and aquades (-) were used as controls. Antiseptic effectiveness test against S. aureus and E. coli bacteria using disc diffusion method. The test results showed that the antiseptic based on gandaria leaves had the ability to inhibit the growth of S. aureus with a concentration of 15% at 2.00 mm and 25% at 4.00 mm. Meanwhile, for E. coli bacteria, antiseptic made from gandaria leaves has an inhibition zone at a concentration of 15% at 1.00 mm. In accordance with the criteria for antiseptic power according to Davis Stout, gandaria leaves which are used as basic ingredients for antiseptics are included in the criteria for very weak antiseptic power (≤5). Therefore, the antiseptic based on gandaria leaves in this study was less effective in inhibiting the growth of S. aureus and E. coli bacteria.
KELIMPAHAN DAN KARAKTERISASI MORFOLOGI BAKTERI COLIFORM PADA KALORA Mahulette, Ferymon; Muskita, Christo; Melay, Stevin
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 8 No 2 (2022): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol8issue2page94-99

Abstract

Background: Kalora is one of the processed fish foods consumed by the people in Maluku. The studies on food safety aspects of this processed food have never been carried out. One of the microbial groups used as an indicator of food safety is Coliform bacteria. This research aims to analyze the number and characteristics of Coliform bacteria in kalora. Methods: This research using three types of fish, i.e. white snapper (Lutjanus sp), scad mackerel (Decapterus sp), and tuna (Thunnus sp) as raw materials of kalora. Analysis of the number of Coliform bacteria used the Most Probable Number (MPN) method by inoculating the sample in lactose broth media, while macroscopic morphological characterization was carried out by streaking a positive tube sample on Eosin methylene blue agar (EMBA) media. Results: The results showed that snapper kalora had the highest MPN value of 9.2 MPN/g, followed by tuna and scad mackerel kalora at 6.2 MPN/g and 3.6 MPN/g, respectively. The characteristics of Coliform bacteria in kalora were varied but dominated by Escherichia and Enterobacter. Conclusion: The third types of kalora has the number of Coliform bacteria below the maximum treshold so it is safe for consumption.
PENGARUH UMUR PANEN SALAK TERHADAP KADAR VITAMIN C BUAH SALAK MERAH (Salacca edulis Reinw) DARI DESA RIRING KECAMATAN TANIWEL KABUPATEN SERAM BAGIAN BARAT Elly, Sovian Sesca; Smith, Alwi; Mahulette, Ferymon
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 9 No 1 (2022): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol9issue1page24-29

Abstract

Background: In Maluku Province, especially in Riring village, there is one variety of salak, namely red salak, which has a nutritional content, namely vitamin C. Vitamin C is a nutrient that acts as an antioxidant. This study aimed to determine the effect of the harvesting age of salak on vitamin C levels in red salak fruit. Methods: This study is an experimental study using a completely randomized design (CRD) with three replications to determine the average vitamin C levels. Analysis of vitamin C levels using the titration method. The data obtained were analyzed using T-test analysis. Results: Based on the study results, the average vitamin C content at harvest age is four months (12.45%), and the average vitamin C content at harvest age is six months (9.53%). The results of the T-test analysis showed that there was a significant effect between the two harvest ages because t-count (19.674)>t-table (2.776). This condition happens because vitamin C is a type of vitamin that is easily oxidized. Conclusion: There is a significant effect between vitamin C levels of red salak fruit aged four months and six months old, where t-count (19.674)> t-table (2.776).
KELIMPAHAN DAN KARAKTERISTIK BAKTERI KOLIFORM PADA BAKASANG BIA GARU (Tridacna gigas L) BERDASARKAN JENIS BAHAN PENGAWET Mahulette, Ferymon; Sarak, Brian; Sangur, Kristin; Rupilu, Ronny
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 9 No 1 (2022): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol9issue1page19-23

Abstract

Background: Giant clam or bia garu is a kind of bivalves in the coral ecosystems. This biota is used as the raw material for processing bakasang by the people of the Babar Islands in Maluku. Preservatives used in the processing of bakasang are depend on the habits of the people. This study aims to determine the number and characteristics of coliform bacteria in clams bakasang based on the type of preservative. Methods:The presumptive test used the Most Probably Number (MPN) method on Lactose broth followed by characterization on Eosin Methylene Blue Agar (EMBA) media. Result: The number of coliform bacteria in clams bakasang using palmyra vinegar was 20 MPN/g while bakasang using salt and vinegar was 150 MPN/g. The macroscopic characterization of coliform bacteria in both types of clams bakasang were metallic green and pink with varying edges and elevations. In bakasang using palmyra vinegar were also found yellow and white coliform bacteria. The Microscopic characterization of coliform bacteria in clams bakasang was Gram negative rods with varied cell arrangement. Conclusion: It is suspected that these bacteria belong to the genera Citrobacter, Enterobacter, and Escherichia.
ANALISIS KADAR KARBOHIDRAT PADA TEH CELUP BERBAHAN DASAR DAUN LAMUN (Enhalus acoroides) Tuapattinaya, Prelly Marsell Jolanda; Mahulette, Ferymon; Warella, Juen Carla; Simal, Rufiati; Ratusehaka, Nopri
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 9 No 2 (2023): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol9issue2page232-236

Abstract

Background: Seagrass is the only flowering plant (angiosperm) that has true root and leaf rhizomes that live submerged in the sea. There are 60 species of seagrass scattered in the world's coastal waters, one type of seagrass that can be used by humans is the seagrass Enhalus acoroides. The economic advantage of Enhalus acoroides leaves can be used as a food ingredient because it has a complete and relatively high nutritional content. Enhalus acoroides leaves can be used as raw material for herbal teas. Herbal teas have a high carbohydrate content, for example in Camellia sinensis tea of ​​0.286%, carbohydrates are one of the chemicals that are needed by the human body. Methods: This study was a descriptive study to determine the value of carbohydrate content in seagrass (Enhalus acoroides) leaf teabags. The research was carried out in March 2022. Sampling of seagrass leaves was carried out in Suli Village, Salahutu District, Central Maluku Regency. The stage of making seagrass teabags was carried out at the Basic Biology Laboratory of FKIP Pattimura University. And the analysis of carbohydrate content was carried out at the Biochemistry Laboratory of the Faculty of Mathematics and Natural Sciences, Pattimura University, Ambon with the type of experimental research. Results: Based on the observations that have been made, the results showed that the carbohydrate content of TL U1 was 5.148%, TL U2 was 5.211%l, TL U3 was 5.165%. With an average carbohydrate of 5.174%. Conclusion: Seagrass leaf teabags (Enhalus acoroides) have a high carbohydrate content and can be a new product that is rich in nutrients.
PENGARUH EKSTRAK KULIT MANGGIS (Garcinia Mangostana L) TERHADAP KADAR FLAVONOID DAN KUALITAS ORGANOLEPTIK NATA DE SOYA Kalkoy, Yosualda; Pattipeilohy, Mery; Sangur, Kristin; Mahulette, Ferymon
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 2 (2025): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue2page237-242

Abstract

Background: Mangosteen (Garcinia mangostana L) is one of Indonesia's tropical fruits. Mangosteen peel extract can be used as a natural colorant for a variety of products such as medicines, cosmetics, beverages and processed foods. Mangosteen peel contains compounds of flavonoids, saponins, tannins, steroids/triterpenoids. Anthocyanins in mangosteen skin can be used as a natural colorant for processed food products nata de soya made from tofu liquid waste. Research Methods: This research was conducted on April 17 - August 21, 2023 at the Basic Chemistry Laboratory of FMIPA and Biology Laboratory of FETT. This type of research is quantitative experimental research, arranged in a completely randomized design. With four treatments of flavonoid levels of 10, 20, 30, 40% mangosteen peel extract and one control. Results: The results showed that there was an effect of mangosteen peel extract as a natural colorant on the flavonoid content of nata de soya. Conclusion: The Smallest Real Difference Test (BNT) at the significance level α 0.05 and the significance level α 0.01 and the results of the BNT Test at the 0.05% level and the 0.01% level for each indicator show that the provision of mangosteen peel extract to the flavonoid content of nata de soya, gives very different results.
Processing and Utilization of Sago palm in Central Moluccas Mahulette, Ferymon; Matulessy, Yoin M; Pattiasina, Eifan B; Rupilu, Marselina R
Local Wisdom Jurnal Ilmiah Kajian Kearifan Lokal Vol. 13 No. 1 (2021): January 2021
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/lw.v13i1.4406

Abstract

Indigenous people in Moluccas consumed sago flour as a main food. It is extracted from the pith of the sago palm (Metroxylon sp) by traditional and modern processing. Sago flour can be processed into various local food products which are local wisdom of the Moluccan. These products are produced by a very diverse community. In addition, stalks and leaves of sago tree can also be used for building construction. This study aims to describe the processing techniques and utilization of sago palm in Central Moluccas. This research was descriptive qualitative. Data was collected from direct observations and documentation from volunteers in several villages only in Central Moluccas, i.e. Hila village (Ambon), Tuhaha and Ouw villages (Saparua), Kamarian and Iha-Luhu villages (West Ceram), and several villages in Geser and Gorom islands (East Ceram). The results showed that sago processing in Central Moluccas was done manually (traditional) or machinery. The processing technique these plants was relatively the same but the processed sago products vary greatly in each village. Sago processed products such as papeda, sagu lempeng, sagu tumbuk, serut, sagu buksona, sagu lakar and sagu fitrah. Besides it flour used as food, stalks and leaves of sago tree are used to build traditional houses, baileo and worship houses. This research was expected to conserve of the pukul sagu tradition and sago processed products to support the tourism in Central Moluccas DOI: https://doi.org/10.26905/lw.v13i1.4406
PRAKTIKUM PEMBUATAN MEDIA PERTUMBUHAN MIKROBA SEMI SINTESIS DAN STERILISASI MENGGUNAKAN ALAT SEDERHANA PADA SISWA SMA NEGERI 28 MALUKU TENGAH Pelamonia, Alamanda; Pattipeilohy, Mery; Mahulette, Ferymon; Sahetapy, Elsa Josma
Jurnal Abdi Insani Vol 12 No 5 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i5.2495

Abstract

Pelaksanaan praktikum di sekolah sering menghadapi kendala, seperti keterbatasan sarana dan prasarana, kurangnya kreativitas dalam perancangan topik, serta minimnya pemanfaatan bahan alami sebagai alternatif dalam praktikum Biologi. SMA Negeri 28 Maluku Tengah mengalami tantangan serupa, terutama dalam praktikum mikrobiologi yang memerlukan alat khusus, kondisi steril, serta biaya media pertumbuhan mikroba yang tinggi. Selain itu, waktu inkubasi yang lama (2–3 hari) semakin membatasi implementasi praktikum ini, sehingga guru kesulitan mengajarkan konsep mikrobiologi secara langsung kepada siswa. Pelaksanaan pengabdian ini bertujuan untuk meningkatkan keterampilan kerja siswa melalui pendampingan dan pelatihan dalam pembuatan media pertumbuhan mikroba semi-sintesis serta sterilisasi menggunakan alat sederhana. PKM dilaksanakan melalui tahapan persiapan, koordinasi dengan sekolah, penyusunan materi, dan penggunaan alat sederhana. Pelaksanaan menggunakan metode ceramah, tanya jawab, dan eksperimen kelompok. Evaluasi dilakukan melalui kuisioner dan umpan balik untuk menilai pemahaman dan kepuasan siswa. Pelatihan ini berhasil meningkatkan keterampilan praktis siswa, memungkinkan mereka untuk memahami dan menerapkan metode pembuatan media serta teknik sterilisasi dengan bahan yang lebih mudah diakses tanpa mengurangi kualitas eksperimen. Selain itu, kegiatan ini berkontribusi pada peningkatan pemahaman siswa terhadap konsep mikrobiologi, penguatan keterampilan berpikir kritis dan pemecahan masalah, serta tumbuhnya minat mereka terhadap sains. Inovasi ini memungkinkan mereka untuk melaksanakan praktikum mikrobiologi dengan alat dan bahan yang lebih terjangkau, tanpa mengurangi kualitas eksperimen yang dilakukan.
Analisis Kualitas Organoleptik Dendeng Kambing dengan Teknik Pengawetan Berbeda di Kecamatan Lakor Kabupaten Maluku Barat Daya Kosaplawan, Martafina; Welerubun, Inggrid; Mahulette, Ferymon
Kalwedo Sains (KASA) Vol 6 No 1 (2025): Kalwedo Sains (KASA), Maret 2025
Publisher : Program Studi Di Luar Kampus Utama Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/kasav6i1p19-26

Abstract

Penelitian ini bertujuan untuk menganalisis kualitas fisik dendeng kambing dengan teknik pengawetan berbeda di Kecamatan Lakor, Kabupaten Maluku Barat Daya. Proses pembuatan dendeng dengan cara menjemur daging kambing di bawah sinar matahari dan cara pengasapan merupakan metode tradisional yang umum digunakan untuk mengawetkan daging di daerah tropis. Proses pembuatan dendeng dilakukan dengan dua metode yaitu penjemuran dengan sinar matahari dan metode pengasapan. Variabel penelitian yang diamati meliputi meliputi:warna, aroma, rasa dan tekstur. Data hasil pengamatan dianalisis menggunakan analisis deskriptif dengan mencari rata-rata dan standar deviasi. Hasil penelitian menunjukkan bahwa teknik pengawetan dengan metode pengasapan menghasilkan kualitas dendeng kambing yang lebih baik, yaitu warna dendeng merah kecoklatan sangat menarik, beraroma daging, rasanya gurih dan manis, serta memiliki tekstur yang lembut dan kenyal, sedangkan teknik pengawetan dengan metode pengeringan sinar matahari menghasilkan warna hitam tidak menarik, sangat berbau amis, rasanya manis namun tidak gurih, serta teksturnya agak padat dan keras.
Characteristics and Potential of Nitrogen-fixing Microbes in the Rhizosphere of Corn and Legume Plants on Kisar Island Mahulette, Ferymon; Pattipeilohy, Mery; Pelamonia, Alamanda
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.9646

Abstract

The Kisar Island community usually plants legumes and corn in one hole called the hapa planting system. This planting system allows interaction between microbes, especially in the rhizosphere that supports plant growth. This study aims to characterize and test the potential of nitrogen-fixing microbes in the rhizosphere of legumes and corn plants using the hapa planting system. Isolation and characterization of nitrogen-fixing microbes using Yeast extract mannitol agar (YEMA) media added with Congo red and bromothymol blue as indicators. The potential of the microbes tested was the ability to dissolve phosphate using Picovskaya media and cellulolytic using Carboxy methyl cellulose (CMC) media. A total of five isolates of nitrogen-fixing microbes were found each in the rhizosphere of corn and mung beans (JKH) and corn and red beans (JKM) with varying numbers and characteristics. The number of microbes in the JKH rhizosphere was 9.5x102 cfu/g lower than the number of microbes in JKM which was 1.5x103 cfu/g. The highest phosphate-dissolving ability was found in isolate KM5 with a phosphate solubility index of 3.14, while the cellulolytic ability was found in isolate KH1 with a cellulolytic index of 0.80. These potential microbes can be developed as starters for developing biofertilizers on less fertile agricultural land in the future.
Co-Authors Antonius Suwanto Awan, Ali Dea Indriani Astuti Elly, Sovian Sesca Hajan, Esmi Irwina, Irwina Kalkoy, Yosualda Kosaplawan, Martafina Kurnia, Tri Santi Lelapary, Claudia Manusiwa, Gellian Gabriella Matulessy, Yoin M Melay, Stevin Mery Pattipeilohy, Mery Muskita, Christo Nikijuluw, Monica NISA RACHMANIA MUBARIK Papilaya, Pamella Mercy Pattiasina, Eifan B Pattiwael, Syane Pelamonia, Alamanda Ratusehaka, Nopri Rupilele, Edy Rupilu, Marselina R Rupilu, Marselina Ribka Rupilu, Ronny Sahetapy, Elsa Josma Sangur, Kristin Sarak, Brian Serpara, Ridolf Simal, Rufiati Smith, Alwi Tuapattinaya, Prelly Marsell Jolanda Warella, Juen Carla Welerubun, Inggrid WIDANARNI WIDANARNI