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All Journal KOMUNITAS: INTERNATIONAL JOURNAL OF INDONESIAN SOCIETY AND CULTURE Local Wisdom : Jurnal Ilmiah Kajian Kearifan Lokal Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Abdi Insani BIOEDUPAT: Pattimura Journal of Biology and Learning Pakem : Jurnal Pengabdian Kepada Masyarakat Rumphius Pattimura Biological Journal Kalwedo Sains (KASA) Berita Biologi
Ferymon Mahulette, Ferymon
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Public Health Social Sciences Other

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Analysis of coliform bacteria contamination in drinking water refill in Ambon City Nikijuluw, Monica; Pattipeilohy, Mery; Mahulette, Ferymon
BIOEDUPAT: Pattimura Journal of Biology and Learning Vol 3 No 2 (2023): Bioedupat : Pattimura Journal of Biology and Learning
Publisher : Program Studi Pendidikan Biologi, Pascasarjana, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/bioedupat.v3.i2.pp125-128

Abstract

In recent years, refillable drinking water businesses have grown rapidly in several cities in Indonesia, including Ambon City. On the one hand, Refillable Drinking Water Depots (DAMIU) support efforts to realize a healthy society by expanding the reach of clean water consumption, On the other hand, DAMIU tend to be problematic when faced with business interests. Feces contain coliform bacteria which is an indicator of water contamination. This study aims to determine the presence of coliform bacteria in Ambon City. The samples used in the study came from three sub-districts of refill drinking water depot sources, namely the Teluk Dalam sub-district, Nusaniwe sub-district, and Baguala sub-district. Microbiological testing of refill drinking water using MPN (most probable number) method. The MPN test results showed that three refill drinking water samples were not contaminated with coliform bacteria because there was no turbidity or gas formation in the Durham tube for gas-forming bacteria. This indicates that the three refill drinking water samples tested microbiologically have good quality and are safe for consumption, because in 100 ml of water no coliform bacteria were found in accordance with Permenkes No. 492/Menkes/Per/IX/2010 regulations on the requirements and supervision of water quality, which states that the microbiological requirements for drinking water are MPN Coliform /100 ml sample = 0. There are no coliform bacteria in the refillable drinking water depot in Ambon City so the water is suitable for consumption
SAINS ITU MENYENANGKAN: ASISTENSI MENGAJAR IPA PADA SMP DI KECAMATAN LEIHITU Mahulette, Ferymon; Hajan, Esmi; Irwina, Irwina
PAKEM : Jurnal Pengabdian Kepada Masyarakat Vol 3 No 1 (2023): Pakem : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/pakem.3.1.1-6

Abstract

Science learning is still difficult to understand for Junior High School students in Leihitu District, Ambon Island. The main cause are the teacher's lack of creativity to designing concrete science lessons and incomplete laboratory facilities. This problem was slightly resolved with practical-based learning in the Lecturer Teaching Assistance of Pattimura University in 2022. The activity was carried out 4 times in class IX students at SMPN 103 Central Maluku and SMP Muhammadyah Mamala on December 13-17 2022. The topic being taught was electricity static and dynamic in life, magnetism and its uses, biotechnology and land and the sustainability of life. The mean scores of the two schools' student satisfaction levels for this activity were 3,401 and 3,408, respectively, in the strongly agree category. The continuation of Lecturer Teaching Assistance needs to be carried out because it is very beneficial to improve the quality of science learning in schools
PELATIHAN PEMBUATAN MEDIA PETUMBUHAN MIKROBA UNTUK MENINGKATKAN WORK SKILL GURU, RESEARCH SKILL SISWA JURUSAN IPA Pattipeilohy, Mery; Mahulette, Ferymon; Pelamonia, Alamanda
PAKEM : Jurnal Pengabdian Kepada Masyarakat Vol 4 No 1 (2024): Pakem : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/pakem.4.1.127-133

Abstract

Permasalahan yang sering dijumpai di sekolah-sekolah terkait pelaksanaan praktikum adalah minimnya peralatan karena kurangnya sarana dan prasana penunjang kegiatan praktikum, kurangnya kreativitas dalam menyusun topik praktikum dan minimnya pengatahuan guru dalam memanfaatkan bahan-bahan alami yang dapat dijadikan sebagai bahan alternatif dalam pelaksanaan proses praktikum. Permasalahan inilah yang dialami oleh guru biologi pada SMA Negeri 1 Seram Bagian Barat, dimana khususnya untuk konsep mikrobiologi tidak di lakukan praktikum, dikarenakan dalam pelaksanaannya membutuhkan alat dan bahan yang banyak juga proses kerja yang harus steril. Tetapi karena alat dan bahan yang tidak lengkap dan bahkan tidak tersedia karena bahan untuk pertumbuhan mikroba cukup mahal dan butuh waktu yang lama dalam proses inkubasi serta pengamatan mikroba (bakteri dan kapang) yakni memerlukan waktu 2-3 hari. Hal ini yang menyebabkan guru mengalami kesulitan untuk melakukan praktikum terkait konsep mikrobiologi sehingga prakitkum belum pernah dilakukan. Oleh karena itu, tujuan dari pelaksanaan pengabdian ini adalah untuk meningkatkan work skill guru dan research skill siswa pada sekolah tersebut melalui pendampingan dan pelatihan menggunakan draft penuntun praktikum. Berdasarkan hasil yang diperoleh diketahui bahwa kemampuan kerja dan keterampilan guru dan juga kemampuan siswa semakin meningkat.
KELIMPAHAN DAN KARAKTERISTIK BAKTERI DALAM BAKASANG BIA GARU (Tridacna gigas L) [Abundance and Characteristics of Bacteria in Bakasang Bia Garu (Tridacna gigas)] Mahulette, Ferymon; Pattipeilohy, Mery; Pelamonia, Alamanda
Berita Biologi Vol 22 No 3 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.697

Abstract

Bakasang bia garu is a clam-based fermented product processed by the people of the Babar Islands, Maluku. Uncontrolled processing allows the presence of unwanted bacteria in these local food products. Preservatives added in the processing of bakasang bia garu are very diverse. This study aims to isolate and characterize lactic acid, halotolerant, and coliform bacteria from bakasang bia garu which are processed using different preservatives. Lactic acid bacteria were isolated using de Man Rogosa and Sharpe Agar (MRSA) media while halotolerant and coliform bacteria used Mannitol Salt Agar (MSA) and Eosin Methylene Blue Agar (EMBA) media, respectively. Characterization was carried out macroscopically and microscopically. The results showed that lactic acid bacteria were only found in bakasang with synthetic salt and vinegar preservatives of 3.5x103 CFU/g which was dominated by Pediococcus pentosaceous. Halotolerant and coliform bacteria have diverse characteristics that belong to the genera Staphylococcus, Escherichia, and Enterobacter. Bakasang bia garu needs to optimize its controlled processing to improve the quality of this local food product in the future
Morphological Characteristics and Abundance of Bacteria in Fried Snack Foods from the Pujasera Canteen, Universitas Pattimura, and Fungi in Spoiled Fruits from Mardika Market, Ambon Pattipeilohy, Merry; Mahulette, Ferymon; Pelamonia, Alamanda
RUMPHIUS Vol 8 No 1 (2026): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv8i1p039-046

Abstract

This study investigated the morphological characteristics and abundance of bacteria and fungi isolated from fried snack foods and spoiled fruits. Bacterial isolates were obtained from bakwan, batagor, and stuffed tofu, while fungal isolates were recovered from spoiled mango, banana, and papaya. Culture-based methods were employed to characterize macroscopic colony morphology and to quantify microbial abundance using the Total Plate Count (TPC) method. The results revealed distinct dominance patterns across different food types. In fried snacks, isolates BKK3, BTK2, and STK5 exhibited the highest TPC values, reaching up to 10⁷ CFU/g, indicating substantial post-processing bacterial contamination. In contrast, spoiled fruits were predominantly colonized by fungal isolates MGK3, BNK3, and PPK4, with TPC values also reaching 10⁷ CFU/g. These fungal isolates displayed typical filamentous mold characteristics, including white-to-green mycelia, granular to rugose textures, and radial and concentric growth patterns. This comparative analysis within a unified study framework demonstrates that difference in food type and intrinsic characteristics-namely ready-to-eat processed products with high environmental exposure versus fresh fruit tissues rich in nutrients and simple sugars-shape contrasting microbial dominance patterns. Fried snacks were primarily dominated by post-processing bacterial contaminants, whereas spoiled fruits were characterized by saprophytic fungal predominance. These findings highlight the critical role of physicochemical properties and handling conditions in determining microbial community dynamics and provide a conceptual basis for microbiological risk assessment in street-vended and fresh food systems. Practically, the results underscore the importance of hygiene control and post-processing management to mitigate microbial hazards and potential mycotoxin risks.
Co-Authors Antonius Suwanto Awan, Ali Dea Indriani Astuti Eifan Boyke Pattiasina Elly, Sovian Sesca Hajan, Esmi Irwina, Irwina Kalkoy, Yosualda Kosaplawan, Martafina Kurnia, Tri Santi Lelapary, Claudia Manusiwa, Gellian Gabriella Matulessy, Yoin M Melay, Stevin Mery Pattipeilohy, Mery Muskita, Christo Nikijuluw, Monica NISA RACHMANIA MUBARIK Papilaya, Pamella Mercy Pattipeilohy, Merry Pattiwael, Syane Pelamonia, Alamanda Ratusehaka, Nopri Rupilele, Edy Rupilu, Marselina R Rupilu, Marselina Ribka Rupilu, Ronny Sahetapy, Elsa Josma Sangur, Kristin Sarak, Brian Serpara, Ridolf Simal, Rufiati Smith, Alwi Tuapattinaya, Prelly Marsell Jolanda Warella, Juen Carla Welerubun, Inggrid WIDANARNI WIDANARNI