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All Journal Teknika BERKALA SAINSTEK KOVALEN: Jurnal Riset Kimia Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) JOURNAL ONLINE OF PHYSICS Jurnal Manajemen & Supervisi Pendidikan Jurnal Penelitian Sains Indonesian Journal of Pure and Applied Chemistry IJEMS (Indonesian Journal of Environmental Management and Sustainability) Share : Journal of Service Learning AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Jurnal Fisika : Fisika Sains dan Aplikasinya INTEGRITAS : Jurnal Pengabdian Studi Kasus Inovasi Ekonomi Reswara: Jurnal Pengabdian Kepada Masyarakat Hydrogen: Jurnal Kependidikan Kimia CHEDS: Journal of Chemistry, Education, and Science Jurnal Al Ulum: LPPM Universitas Al Washliyah Medan Makara Journal of Technology Jurnal Ilmu dan Inovasi Fisika Sainteks: Jurnal Sain dan Teknik Babakti : Journal of Community Engangement
Teguh Pambudi
Universitas Singaperbangsa Karawang
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Determination of the Kinetic Parameters of Cholesterol Oxidation using Cholesterol Oxidase from Streptomyces sp. Perdani, Meka Saima; Hermansyah, Heri; Sahlan, Muhamad; Putri, Dwini Normayulisa; Pambudi, Teguh; Hasibuan, Anggi Khairina Hanum
Makara Journal of Technology Vol. 28, No. 3
Publisher : UI Scholars Hub

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Abstract

Cholesterol oxidase (CO) was successfully produced from Streptomyces sp. via the submerged fermentation method, and 69 U/mL enzyme activity was obtained. This study aimed to determine cholesterol oxidation kinetics and the production of CO as a catalyst. The enzyme was diluted to 0.15, 0.075, and 0.00375 U/mL for the oxidation reaction. The substrate was also prepared in three concentrations: 3.23, 6.46, and 12.93 mM. The optimization of conditions for enzymatic cholesterol oxidation was investigated through measurement of the effect of initial cholesterol and enzyme concentrations. Cholesterol concentration was rapidly measured via high-performance liquid chromatography (HPLC). The kinetics of CO were modeled using the first-order irreversible reaction. An enzymatic kinetic model was derived, and it was verified using experimental data and sensitivity analysis. Based on the experiment, the highest enzyme concentrations of crude and commercial CO can oxidize the substrate up to 84% within 240 min. However, the oxidation reaction showed a slightly different behavior in the early 60 min, and crude CO exhibited a slower substrate oxidation. The kinetic rate constant obtained by Euler’s method reached 1.0 x 10−3/min and 1.41 x 10−3/min for 0.15 U/mL crude and commercial CO, respectively.
INOVASI PENGELOLAAN LIMBAH SEKAM PADI MENJADI ARANG BRIKET SEBAGAI SOLUSI ENERGI HIJAU DI DESA KARANGTANJUNG KARAWANG Eri Widianto; Kardiman Kardiman; Farradina Choria Suci; Siswadi Siswadi; Rizal Hanifi; Najla Salsabila; Hilman Imadul Umam; Teguh Pambudi
INTEGRITAS : Jurnal Pengabdian Vol 9 No 2 (2025): AGUSTUS - DESEMBER
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat - Universitas Abdurachman Saleh Situbondo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/integritas.v9i2.7478

Abstract

Karangtanjung Village in Karawang Regency produces a substantial amount of rice husk waste, yet its utilization remains limited, with most of it being disposed of through open burning. This Community Service Program aims to enhance the skills of the Sukamanah II Farmers Group in converting rice husk waste into charcoal briquettes through technical training and the implementation of appropriate technology. The effectiveness of the program was evaluated using pre-test and post-test instruments to assess participants’ knowledge improvement. The results indicate an increase of 27–35% in average scores after the training sessions. The effectiveness analysis using the Normalized Gain (N-Gain) method shows that most participants achieved moderate to high improvement, with N-Gain values ranging from 0.33 to 1.00. Approximately 30–40% of participants reached the high-effectiveness category (N-Gain > 0.7), while the rest fell into the moderate category. These findings confirm that the combination of theoretical introduction, demonstration, and hands-on practice is effective in improving participants’ technical understanding of briquette production. Beyond cognitive improvement, the program also produced tangible outputs, including a briquette press machine and standardized production SOP modules. Early impacts include reduced open burning, increased productivity in biomass utilization, and emerging initiatives to establish a village-level briquette business unit. Overall, this program contributes to strengthening circular economy practices and promoting green energy innovation based on agricultural waste.
IMPLEMENTASI EKONOMI SIRKULAR MELALUI TEKNIK BUDIDAYA MAGGOT DI TPS3R TELUKJAMBE KARAWANG Putri Masruroh; Teguh Pambudi; Venny Ulya Bunga; Hilman Imadul Umam
Studi Kasus Inovasi Ekonomi Vol. 9 No. 02 (2025)
Publisher : Program Studi Manajemen, Fakultas Ekonomi dan Bisnis Universitas Muhammadiyah Malang

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Abstract

Kapasitas pengelolaan sampah di Kabupaten Karawang tidak memadai disebabkan oleh tingginya volume sampah harian. Dari 1200 ton sampah yang dihasilkan, hanya 350 ton sampah yang bisa diangkut ke Tempat Pembuangan Akhir (TPA). Sisa sampah yang tidak terangkut, diolah di Tempat Pengelolaan Sampah Reuse, Reduce, dan Recycle (TPS3R). Salah satu TPS3R yang ada di Kabupaten Karawang adalah TPS3R BUMDES Berkah Bersama di Desa Telukjambe yang memiliki kapasitas pengolahan sampah rumah tangga sebesar 31,14% dari 8266 kg sampah yang ada. Keterbatasan pengelolaan sampah ini dapat menjadi masalah sosial karena timbunan sampah akan terus bertambah dengan bertambahnya populasi. Untuk itu tim pengabdian dari Fakultas Teknik Universitas Singaperbangsa Karawang telah mengadakan pelatihan pengelolaan sampah rumah tangga, pelatihan pengolahan sampah organik menjadi substrat untuk pakan maggot BSF, pembuatan fasilitas budidaya maggot BSF (rumah lalat, biopond, wadah telur) dan pelatihan tentang manajemen siklus hidup maggot BSF. Sasaran dari kegiatan pengabdian ini adalah petugas TPS3R BUMDES Berkah Bersama mampu melakukan budidaya maggot BSF secara berkelanjutan. Berdasarkan hasil pelatihan, diperoleh nilai rata-rata posttest (66,67) lebih besar dari nilai rata-rata pretest (32). Hasil uji t-berpasangan nilai pretest dan postest berbeda nyata (t=6,98, p< 5%). Hasil ini menunjukkan bahwa pelatihan berpengaruh nyata terhadap pengetahuan petugas TPS3R BUMDES Berkah dalam budidaya maggot BSF.
Co-Authors Alfieta Rohmaful Aeni ANGGI KHAIRINA HANUM HASIBUAN Anjani, Cindy Salsabilla Armin, Edmund Ucok Asyafa, Amalia Cantika Athirah, Febriana Azhar Aulia Wahyuningtyas Eri Widianto Farradina Choria Suci Fauzie, Azis Kemal Fitri Yuliasari Hakim , Muhammad Fahmi Hakim, Muhammad Fahmi Hakim Hakimah Nurjanah Hanan, Ihsan Sofyan Heri Hermansyah Hilman Imadul Umam Imadul Umam, Hilman Kardiman Marsah Rahmawati Utami Meka Saima Perdani Meka Saima Perdani Najla Salsabila Nandira, Rantika Sekar Nur Fadila, Salsabil Intan Pahlevi, Rio Ramdhan Pangestoe, Safina Handayani Perdani, Meka Saima Perdani, Meka Saiman Putri Masruroh Putri, Dwini Normayulisa Putri, Fajar Amelia Rachmawati Rahmat Hidayat Rahmatullah, Alzachry Redhaqa Rizal Hanifi SAHLAN, MUHAMAD Salsabila, Riesya Sagita Salsabila Saniatun Wilda Savitri, Meila Shakila, Tasya Nur Siswadi Siswadi Sonia, Gita Venny Ulya Bunga Wahyuningtyas, Aulia Widianto, Eri YULIASARI, FITRI Zuhri, Alifa Rahma