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All Journal Teknika BERKALA SAINSTEK KOVALEN: Jurnal Riset Kimia Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) JOURNAL ONLINE OF PHYSICS Jurnal Manajemen & Supervisi Pendidikan Jurnal Penelitian Sains Indonesian Journal of Pure and Applied Chemistry IJEMS (Indonesian Journal of Environmental Management and Sustainability) Share : Journal of Service Learning AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Jurnal Fisika : Fisika Sains dan Aplikasinya Reswara: Jurnal Pengabdian Kepada Masyarakat Hydrogen: Jurnal Kependidikan Kimia CHEDS: Journal of Chemistry, Education, and Science Jurnal Al Ulum: LPPM Universitas Al Washliyah Medan Makara Journal of Technology Jurnal Ilmu dan Inovasi Fisika Sainteks: Jurnal Sain dan Teknik Babakti : Journal of Community Engangement
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Universitas Singaperbangsa Karawang
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Determination of the Kinetic Parameters of Cholesterol Oxidation using Cholesterol Oxidase from Streptomyces sp. Perdani, Meka Saima; Hermansyah, Heri; Sahlan, Muhamad; Putri, Dwini Normayulisa; Pambudi, Teguh; Hasibuan, Anggi Khairina Hanum
Makara Journal of Technology Vol. 28, No. 3
Publisher : UI Scholars Hub

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Abstract

Cholesterol oxidase (CO) was successfully produced from Streptomyces sp. via the submerged fermentation method, and 69 U/mL enzyme activity was obtained. This study aimed to determine cholesterol oxidation kinetics and the production of CO as a catalyst. The enzyme was diluted to 0.15, 0.075, and 0.00375 U/mL for the oxidation reaction. The substrate was also prepared in three concentrations: 3.23, 6.46, and 12.93 mM. The optimization of conditions for enzymatic cholesterol oxidation was investigated through measurement of the effect of initial cholesterol and enzyme concentrations. Cholesterol concentration was rapidly measured via high-performance liquid chromatography (HPLC). The kinetics of CO were modeled using the first-order irreversible reaction. An enzymatic kinetic model was derived, and it was verified using experimental data and sensitivity analysis. Based on the experiment, the highest enzyme concentrations of crude and commercial CO can oxidize the substrate up to 84% within 240 min. However, the oxidation reaction showed a slightly different behavior in the early 60 min, and crude CO exhibited a slower substrate oxidation. The kinetic rate constant obtained by Euler’s method reached 1.0 x 10−3/min and 1.41 x 10−3/min for 0.15 U/mL crude and commercial CO, respectively.
Co-Authors Aeni, Alfieta Rohmaful Alfieta Rohmaful Aeni ANGGI KHAIRINA HANUM HASIBUAN Anjani, Cindy Salsabilla Armin, Edmund Ucok Asyafa, Amalia Cantika Athirah, Febriana Azhar Aulia Wahyuningtyas Fauzie, Azis Kemal Fitri Yuliasari Hakim , Muhammad Fahmi Hakim, Muhammad Fahmi Hakim Hakimah Nurjanah Hanan, Ihsan Sofyan Heri Hermansyah Hilman Imadul Umam Imadul Umam, Hilman Marsah Rahmawati Utami Meka Saima Perdani Meka Saima Perdani Nandira, Rantika Sekar Nur Fadila, Salsabil Intan Pahlevi, Rio Ramdhan Pangestoe, Safina Handayani Perdani, Meka Saima Perdani, Meka Saiman Putri, Dwini Normayulisa Putri, Fajar Amelia Rachmawati Rahmat Hidayat Rahmatullah, Alzachry Redhaqa SAHLAN, MUHAMAD Salsabila, Riesya Sagita Salsabila Saniatun Wilda Savitri, Meila Shakila, Tasya Nur Sonia, Gita Wahyuningtyas, Aulia Widianto, Eri YULIASARI, FITRI Zuhri, Alifa Rahma