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All Journal Media Kesehatan Masyarakat Indonesia Majalah Kedokteran Bandung Health Science Journal of Indonesia Nexus Pendidikan Kedokteran dan Kesehatan Nexus Kedokteran Komunitas Nexus Biomedika Nexus Kedokteran Translasional WARTA Biomedika Jurnal Kebidanan Smart Medical Journal AgriHealth: Journal of Agri-food, Nutrition and Public Health Proceeding ISETH (International Summit on Science, Technology, and Humanity) Plexus Medical Journal
Ayusari, Amelya Augusthina
Nutrition Department, Faculty Of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia Dr. Moewardi Hospital Surakarta City, Indonesia Nutrition, Metabolism And Medicine Research Group
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Education Engineering Environmental Science Health Professions Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Nexus Kedokteran Translasional

 1 
The Effect of Alkaline Water on Blood Glucose Diabetic White Rates. Ramadhani, Dhia; Ediningsih, Endang; Ayusari, Amelya Augusthina
Nexus Kedokteran Translasional Vol 4, No 1 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.207 KB)

Abstract

Background: Alkaline water has high pH level and work as antioxidant that could repair the damaged pancreatic beta cells caused by alloxan. This research aimed to know the effect of alkaline water on blood glucose in diabetic white rats induced by alloxan. Methods: This was an experimental research with pre- and post-test group design. The subjects were thirty Wistar male white rats selected by a simple random technique. They were divided into 2 groups, control group (aquadest group), and treatment group given alkaline water dosed 23 ml perday. Blood glucose were measured by stript test at the first day as GDP 1, at the fifth day after induced by alloxan as GDP 2, and the last, after treatment at the twelfth day as GDP 3. The data were analyzed by Anova test followed by Post Hoc Test using SPSS for Windows Release 20 program. Results: This research showed that there was significant difference of GDP 3 betweenaquadest and alkaline group (p=0,00) by independent t test.There was no significant difference between GDP 1 and GDP 3 both group by dependent t test. Conclusion: It concluded that alkaline water was able to decrease the blood glucose level on diabetic white rates induced by alloxan. Keywords: alkaline water, blood glucose level, antioxidant 
In Vitro Inhibition Effect of Coconut Husk Ethanol Extract towards the Growth of Escherichia coli and Staphylococcus aureus from Surgical Site Infection Jatiningrum, Alifiana; ., Maryani; Ayusari, Amelya Augusthina
Nexus Kedokteran Translasional Vol 4, No 2 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.207 KB)

Abstract

Background: Surgical site infection was defined clinically, namely the existence of purulent discharge around the wound or wound cellulitis in operation. Coconut husk are known to has tannin and flavonoid as antibacterial properties. This study aimed to determine In Vitro effect of coconut husk (Cocos nucifera L.) ethanol extract towards the growth of Escherichia coli and Staphylococcus aureus from surgical site infection. Methods: This research is an experimental laboratory (post test only control group design) with a non-probability sampling technique namely consecutive sampling. Research conducted at the Microbiology Laboratory FK UNS and sampling conducted in the Microbiology Laboratory Dr. Moewardi Hospital. The independent variables are the coconut husk ethanol extract at a concentration of 1%, 20%, 40%, 60%, and 80%. Subjects were Escherichia coli and Staphylococcus aureus of surgical site infection standardized with Mc Farland 0.5. Test sensitivity on Muller-Hinton medium diffusion method with antibiotics cefoperazone/sulbactam and vancomycin as a positive control and DMSO 2% as negative control. Research results are tested with the Kruskal-Wallis test followed by Mann-Whitney test. Results: Coconut husk ethanol extract can’t inhibit the growth of Escherichia coli but can inhibit the growth of Staphylococcus aureus. Based on the results of the Kruskal-Wallis test that at least there is a significant difference in inhibition between the two treatment groups (p <0.005). The results of the Mann-Whitney test showed that there were significant differences in inhibition (p <0.05) in all treatment groups, except in the group with the inhibition of the extract concentration of 40% with 60% extract concentration did not reveal any significant differences in inhibition (p > 0.05). Conclusion: Coconut husk ethanol extract can’t inhibit the growth of Escherichia coli but can inhibit the growth of Staphylococcus aureus from surgical site infection by In Vitro. Keywords: Surgical site infection, Escherichia coli, Staphylococcus aureus, coconut husk
Pomegranate Extract Does Not Inhibit Sodium Glucose co-Transporter 2 Protein in Vero Cells Ulfia, Mila; Indarto, Dono; Ayu Sari, Amelya Augusthina; Suselo, Yuliana Heri
Nexus Kedokteran Translasional Vol 6, No 1 (2017): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.209 KB)

Abstract

Backgrounds: Mutation of SLC5A2 gene which encodes sodium glucose co-transporter2 (SGLT2) protein enhances glucose reabsorption on the kidney tubule in some patients with type 2 diabetes (DMT2). Dapagliflozine an oral antidiabetic drug, inhibits SGLT2 activity. Ellagic acid is able to inhibit SGLT2 protein in silico and highly found in pomegranate fruits. The aim of this study was to investigate the effect of pomegranate extracts on glucose levels in a model cell of African green monkey (Vero cell line). Methods: This study was an experimental laboratory with posttest only control group design. 1 x 106 Vero cells perwell were used in five experimental groups: negative control 1 (KKn1), KKn with 20% glucose (KKn2), positive control with dapagliflozine (KKp), ethanol and diethyl ether extract of pomegranate peel (KEDA), methanol extract of pomegranate seeds (BMA). Vero cells were then treated with 125 ppm pomegranate extracts (KEDA and BMA) and incubated for 24 hours. Cell morphology was observed under an inverted microscope with 100 x magnification. Glucose levels in Vero cells were measured using spectrophotometer. Collected data was analyzed descriptively. Result: Morphology of Vero cells was oval, soliter and centered nucleus and did not change during incubation with pomegranate extracts. Glucose levels in Vero cells treated with BMA (28.5 mg/dL) and KEDA (29 mg/dL) were higher than glucose levels in control groups KKp, KKn1, and KKn2 (2.5, 6.5 and 8 mg/dL respectively). Conclusion; Pomegranate extracts do not inhibit SGLT2 protein and increase glucose levels in Vero cells. Purification of pomegranate extracts is required for further investigation of the capability of ellagic acid inhibiting SGLT2 protein. Keywords: Ellagic acid, glucose level, pomegranate, SGLT2, type 2 diabetes.
Pomegranate Extract Does Not Inhibit Sodium Glucose co-Transporter 2 Protein in Vero Cells Mila Ulfia; Dono Indarto; Amelya Augusthina Ayu Sari; Yuliana Heri Suselo
Nexus Kedokteran Translasional Vol 6, No 1 (2017): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.209 KB)

Abstract

Backgrounds: Mutation of SLC5A2 gene which encodes sodium glucose co-transporter2 (SGLT2) protein enhances glucose reabsorption on the kidney tubule in some patients with type 2 diabetes (DMT2). Dapagliflozine an oral antidiabetic drug, inhibits SGLT2 activity. Ellagic acid is able to inhibit SGLT2 protein in silico and highly found in pomegranate fruits. The aim of this study was to investigate the effect of pomegranate extracts on glucose levels in a model cell of African green monkey (Vero cell line). Methods: This study was an experimental laboratory with posttest only control group design. 1 x 106 Vero cells perwell were used in five experimental groups: negative control 1 (KKn1), KKn with 20% glucose (KKn2), positive control with dapagliflozine (KKp), ethanol and diethyl ether extract of pomegranate peel (KEDA), methanol extract of pomegranate seeds (BMA). Vero cells were then treated with 125 ppm pomegranate extracts (KEDA and BMA) and incubated for 24 hours. Cell morphology was observed under an inverted microscope with 100 x magnification. Glucose levels in Vero cells were measured using spectrophotometer. Collected data was analyzed descriptively. Result: Morphology of Vero cells was oval, soliter and centered nucleus and did not change during incubation with pomegranate extracts. Glucose levels in Vero cells treated with BMA (28.5 mg/dL) and KEDA (29 mg/dL) were higher than glucose levels in control groups KKp, KKn1, and KKn2 (2.5, 6.5 and 8 mg/dL respectively). Conclusion; Pomegranate extracts do not inhibit SGLT2 protein and increase glucose levels in Vero cells. Purification of pomegranate extracts is required for further investigation of the capability of ellagic acid inhibiting SGLT2 protein. Keywords: Ellagic acid, glucose level, pomegranate, SGLT2, type 2 diabetes.
In Vitro Inhibition Effect of Coconut Husk Ethanol Extract towards the Growth of Escherichia coli and Staphylococcus aureus from Surgical Site Infection Alifiana Jatiningrum; Maryani .; Amelya Augusthina Ayusari
Nexus Kedokteran Translasional Vol 4, No 2 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.207 KB)

Abstract

Background: Surgical site infection was defined clinically, namely the existence of purulent discharge around the wound or wound cellulitis in operation. Coconut husk are known to has tannin and flavonoid as antibacterial properties. This study aimed to determine In Vitro effect of coconut husk (Cocos nucifera L.) ethanol extract towards the growth of Escherichia coli and Staphylococcus aureus from surgical site infection. Methods: This research is an experimental laboratory (post test only control group design) with a non-probability sampling technique namely consecutive sampling. Research conducted at the Microbiology Laboratory FK UNS and sampling conducted in the Microbiology Laboratory Dr. Moewardi Hospital. The independent variables are the coconut husk ethanol extract at a concentration of 1%, 20%, 40%, 60%, and 80%. Subjects were Escherichia coli and Staphylococcus aureus of surgical site infection standardized with Mc Farland 0.5. Test sensitivity on Muller-Hinton medium diffusion method with antibiotics cefoperazone/sulbactam and vancomycin as a positive control and DMSO 2% as negative control. Research results are tested with the Kruskal-Wallis test followed by Mann-Whitney test. Results: Coconut husk ethanol extract cant inhibit the growth of Escherichia coli but can inhibit the growth of Staphylococcus aureus. Based on the results of the Kruskal-Wallis test that at least there is a significant difference in inhibition between the two treatment groups (p <0.005). The results of the Mann-Whitney test showed that there were significant differences in inhibition (p <0.05) in all treatment groups, except in the group with the inhibition of the extract concentration of 40% with 60% extract concentration did not reveal any significant differences in inhibition (p > 0.05). Conclusion: Coconut husk ethanol extract cant inhibit the growth of Escherichia coli but can inhibit the growth of Staphylococcus aureus from surgical site infection by In Vitro. Keywords: Surgical site infection, Escherichia coli, Staphylococcus aureus, coconut husk
The Effect of Alkaline Water on Blood Glucose Diabetic White Rates. Dhia Ramadhani; Endang Ediningsih; Amelya Augusthina Ayusari
Nexus Kedokteran Translasional Vol 4, No 1 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.207 KB)

Abstract

Background: Alkaline water has high pH level and work as antioxidant that could repair the damaged pancreatic beta cells caused by alloxan. This research aimed to know the effect of alkaline water on blood glucose in diabetic white rats induced by alloxan. Methods: This was an experimental research with pre- and post-test group design. The subjects were thirty Wistar male white rats selected by a simple random technique. They were divided into 2 groups, control group (aquadest group), and treatment group given alkaline water dosed 23 ml perday. Blood glucose were measured by stript test at the first day as GDP 1, at the fifth day after induced by alloxan as GDP 2, and the last, after treatment at the twelfth day as GDP 3. The data were analyzed by Anova test followed by Post Hoc Test using SPSS for Windows Release 20 program. Results: This research showed that there was significant difference of GDP 3 betweenaquadest and alkaline group (p=0,00) by independent t test.There was no significant difference between GDP 1 and GDP 3 both group by dependent t test. Conclusion: It concluded that alkaline water was able to decrease the blood glucose level on diabetic white rates induced by alloxan. Keywords: alkaline water, blood glucose level, antioxidant
Co-Authors ., Maryani ., Suyatmi Adigama Priamas Agatha Adika Putri, Claudia Ahadina Rahma Zulardi Ainal Fadly Alamanda Murasmita Alifah, Noor Alifiana Jatiningrum Amalina Shabrina Arifah, Umi Azizah, Shofura Bella Nadhifa Budiyanti Wiboworini Budiyanti Wiboworini Budiyanti Wiboworini Budiyanti Wiboworini Budiyanti Wiboworini Chofsoh Z, Anifa Coana Sukmagautama Desiana, Dyah Tantry Deyona Annisa Putri Dhia Ramadhani Diffah Hanim Doewes, Muchsin Dono Indarto DONO INDARTO Dwi Rahayu Dwi Rahayu Dwi Rahayu Dwi Rahayu Dyah Tantry Desiana Ediningsih, Endang Ediningsih, Endang Endang Dewi Lestari, Endang Dewi Endang Ediningsih Esha R, Andrini Fadly, Ainal Gisti Respati Riyanti Graciella Angelica Lucas Hanifah Fathimatuzzahrah Hanim, Diffah Hastami, Yunia Intaniar Intaniar Jatiningrum, Alifiana Joko Sudarsono Joko Sudarsono Joko Sudarsono Kisrini Kisrini Kisrini Kisrini Kusmadewi Eka Damayanti Kusmadewi Eka Damayanti Kusmadewi Eka Damayanti Latifah ., Latifah Lestari, Endang Dewi Lilik Wijayanti M. Yogi Riyantama Isjoni Maryani . Mashuri, Yusuf Ari Maulina, Rufidah Mila Ulfia Monica Fradisha Zukhri Muchsin Doewes Muhammad Daffa Ardiawan Murasmita, Alamanda Myrtha, Risalina Noviyati Rahardjo Putri Nur Aini, Ardelia Priamas, Adigama Purbaningrum, Ayunita Putri, Deyona Annisa R AJ Sri Wulandari Ramadhani, Dhia Ratna Kusumawati Ratna Kusumawati Risalina Myrtha Risalina Myrtha Risalina Myrtha Risalina Myrtha Riyanti, Gisti Respati Siti Nurhidayati Sukmagautama, Coana Sulistyani Kusumaningrum Suselo, Yuliana Heri Suselo, Yuliana Heri Sutartinah Sri Handayani Sutartinah Sri Handayani Sutartinah Sri Handayani Suyatmi . Ulfia, Mila Ulul Albab umi arifah, umi Widardo Widardo Widardo Widardo Widardo Widardo Widardo Widardo Widardo Widardo widardo, Widardo Wulandari, R AJ Sri Yulia Lanti Yulia Lanti Yulia Lanti Yulia Lanti Retno Dewi yulia lanti retno dewi Yuliana Heri Suselo Zukhri, Monica Fradisha Zulardi, Ahadina Rahma