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Aktivitas Penghambatan Bakteri Kitinolitik asal Limbah Pengolahan Udang Lampung terhadap Fusarium sp. dan Phytopthora sp. Suryanti, Erma; Chusniasih, Dewi; Mulyana, Jeane Siswitasari; Rahmah, Nadia; Mulyadi, Salsa Pratiwi; Istiadi, Khaerunissa Anbar
Jurnal Biologi dan Pembelajarannya (JB&P) Vol 12 No 2 (2025): OKTOBER 2025
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/jbp.v12i2.25907

Abstract

Organisme Pengganggu Tanaman (OPT) seperti Fusarium sp. dan Phytophthora sp. merupakan salah satu faktor yang menyebabkan penurunan produktivitas dan kegagalan panen tanaman hortikultura. Untuk mengatasi serangan OPT, petani melakukan pengendalian jamur patogen dengan menyemprotkan fungisida kimia. Namun, penggunaan fungisida sintesis/kimiawi yang tidak terkontrol dapat menyebabkan pencemaran serta resistensi pada OPT. Oleh sebab itu, diperlukan alternatif pengendalian yang lebih aman dengan biokontrol menggunakan bakteri kitinolitik. Bakteri kitinolitik akan mendegradasi kitin pada dinding sel jamur secara spesifik sehingga jamur tidak dapat berkembang dan mengalami kematian. Pada studi ini, dilakukan proses isolasi bakteri kitinolitik dari limbah unit pengolahan udang wilayah Lampung sehingga memiliki potensi penemuan bakteri kitinolitik dengan aktivitas degradasi kitin yang berbeda. Isolat bakteri kitinolitik potensial akan diujikan efektivitasnya sebagai antifungi patogen pada kapang model Fusarium sp. dan Phytophthora sp. secara in vitro. 12 isolat asal limbah pengolahan udang diuji aktivitas kitinolitiknya dengan aktivitas kitinolitik 0,37 – 19,7. Isolat bakteri diketahui mampu menghambat pertumbuhan fungi dengan indeks penghambatan tertinggi oleh isolat  C.68 sebesar 60,5% terhadap Fusarium sp. dan isolat S6.3 sebesar 72,68% terhadap Phytopthora sp.
Peningkatan Nilai Tambah Kakao Lokal Desa Pahawang melalui Pelatihan Produksi Sabun Kakao Sari, Dian Anggria; Istiadi, Khaerunissa Anbar; Suryanti, Erma; Chusniasih, Dewi; Imaniar, Lisana Husna; Ningsih, Ningsih
BERDAYA: Jurnal Pendidikan dan Pengabdian Kepada Masyarakat Vol 8 No 1 (2026)
Publisher : LPMP Imperium

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36407/berdaya.v8i1.1792

Abstract

Pahawang Village is an island with strong marine tourism potential in Lampung. In addition to working as fishermen, many villagers also cultivate cocoa as an agricultural commodity. To date, cocoa beans have only been sold in dried form and have not been processed into other value-added products. In order to increase value added and support the village’s creative economy, product innovations derived from cocoa with high market value and simple production technology are needed. Cocoa beans contain active compounds such as catechins, anthocyanins, and natural fats that function as antioxidants and skin moisturizers. The purpose of this activity was to increase the value of cocoa by processing it into economically valuable soap. The method involved socialization and hands-on practice on cocoa soap production. The results showed a significant increase in community knowledge, reaching 92.8%, regarding the production and packaging of creative cocoa-based products. The activity of processing cocoa into soap had a positive impact on the people of Pahawang Village. With the new skills acquired, the community is expected to be able to independently develop cocoa-based home industries, thereby increasing income and economic welfare. The cocoa soap products also have the potential to become a flagship village product that supports nature tourism in Pahawang Village through the provision of souvenirs.
Isolation and screening of halotolerant protease-producing bacteria from Lampung fermented shrimp paste Istiadi, Khaerunissa Anbar; Ningsih; Suryanti, Erma; Imaniar, Lisana Husna
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.14593

Abstract

Protease are class of degradative enzymes that hydrolyze proteins, breaking its peptide bond, into smaller peptides and amino acids. Microbial proteases have gained increasing attention due to its productivity and utilization in various industries. Proteolytic bacteria derived from fermented shrimp paste from Lampung have great potential to meet this need. However, studies on these bacteria are still limited, so further research is needed to explore and optimally utilize their potential. This study aims to explore the diversity of proteolytic bacteria from Lampung terasi.. The methods employed included isolation stages using Nutrient Agar supplemented with 5% and 10% NaCl (w/v), followed by proteolytic screening properties using Skim Milk Agar media. A total of 20 bacterial isolates were obtained from terasi using Nutrient Agar supplemented with 5% (w/v) NaCl, and 15 isolates were obtained from Nutrient Agar (NA)  supplemented with 10% (w/v) NaCl. Screening results showed that 85% and 67% of the isolates obtained from NA supplemented with 5% and 10% NaCl, respectively, exhibited proteolytic activity. Halotolerant bacterial isolates isolated using NA + 5% NaCl exhibited a proteolytic index ranging from 0.3 – 3.0, while those isolated with NA + 10% NaCl showed values ranging from 0.18 to 3.06. The screening results revealed that isolates BTCL5-07 and BTCL10-05 exhibited the highest proteolytic activity, with proteolytic indices of 3.0 and 3.06, respectively. These findings indicate that halotolerant bacteria from Lampung terasi represent a promising source of potential proteolytic enzymes. Further research is required to optimize enzyme production.