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Edukasi Pola Makan Sehat Mencegah Makanan dan Minuman Mengandung Cemaran Mikroba Peri Peri; Monica Suryani; Devina Chandra
Jurnal Pengabdian Masyarakat Indonesia Sejahtera Vol. 4 No. 4 (2025): Desember: Jurnal Pengabdian Masyarakat Indonesia Sejahtera
Publisher : STAI YPIQ BAUBAU, SULAWESI TENGGARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59059/jpmis.v4i4.2940

Abstract

In general, school students buy snacks in the school canteen or street vendors (PKL) around the school. 2 The types of food sold are varied, ranging from healthy snacks to unhealthy snacks so that they can attract students to consume school snacks. Healthy snacks are foods that have sufficient and balanced nutrition and do not contain (contaminated) elements that can harm or damage health. This community service activity aims to educate healthy eating patterns to prevent food and drinks containing microbial contamination at Mariana Catholic High School, Medan. The method used is an educational approach through health counseling, interactive discussions, and demonstrations of hygienic practices, especially correct handwashing techniques and the importance of maintaining personal hygiene and the school environment. This activity was attended by 40 students as participants. The evaluation of the activity focused on one main indicator, namely student participation in hygienic behavior practices as a form of direct application of the material that has been given. The results of the activity showed that all participants (100%) followed the demonstrated hygienic practices, which indicates a very high level of participation and enthusiasm. These findings indicate that educational methods accompanied by hands-on practice are effective in increasing understanding and encouraging the adoption of clean and healthy living behaviors. Community service activities at Mariana Catholic High School in Medan have been shown to increase students' knowledge about the dangers of microbial contamination and the development of hygienic behaviors in daily life. Structured and ongoing education is an effective preventive measure to create a healthy and clean school environment.
Identifikasi Bakteri dan Jamur pada Makanan dan Minuman Menggunakan Metode Polymerase Chain Reaction (PCR) dan Analisis Deoxyribonucleic Acid (DNA) Molekuler Devina Chandra; Manuppak Irianto Tampubolon; Monica Suryani; Supartiningsih Supartiningsih
Jurnal Ilmiah Kedokteran dan Kesehatan Vol. 5 No. 3 (2026): September: Jurnal Ilmiah Kedokteran dan Kesehatan
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/klinik.v5i3.7660

Abstract

Contamination of food and beverages by pathogenic bacteria and spoilage fungi remains a major public health concern, particularly in traditional and street-vended products where hygienic control is often inadequate. Conventional culture-based identification methods are time-consuming and often fail to detect slow-growing or non-culturable microorganisms. This study aimed to identify bacterial and fungal contaminants in food and beverage samples using Polymerase Chain Reaction (PCR) combined with molecular Deoxyribonucleic Acid (DNA) analysis. Samples were collected from local food vendors, and total genomic DNA was extracted using a commercial extraction kit. Bacterial identification targeted the 16S rRNA gene, while fungal identification targeted the Internal Transcribed Spacer (ITS) region, both amplified by PCR and visualized by agarose gel electrophoresis. Amplicons were sequenced and compared with reference sequences in the GenBank database using the Basic Local Alignment Search Tool (BLAST). The results revealed the presence of pathogenic and spoilage microorganisms, including Escherichia coli, Staphylococcus aureus, Salmonella sp., and Aspergillus species, indicating inadequate sanitation during food handling and storage. These findings confirm that PCR-based molecular analysis provides a rapid, sensitive, and specific approach for detecting microbial contamination in food and beverages.
FABRIKASI RISET ANCAMAN SERIUS BAGI INTEGRITAS PERGURUAN TINGGI DI INDONESIA Cheryl Nathania; Devina Chandra; Felicia Jacinta Ivanka Anter; Nicole Eugenia Yuri; Syamhaikel Pavel Budiman
Jurnal Riset Multidisiplin Edukasi Vol. 3 No. 7 (2026): Jurnal Riset Multidisiplin Edukasi (Juli 2026)
Publisher : PT. Hasba Edukasi Mandiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71282/jurmie.v3i7.2456

Abstract

Fabrikasi riset merupakan salah satu bentuk pelanggaran etika penelitian yang mengancam integritas akademik dan kredibilitas perguruan tinggi di Indonesia. Penelitian ini bertujuan menganalisis faktor penyebab, dampak, serta upaya pencegahan praktik fabrikasi riset melalui pendekatan studi pustaka. Metode yang digunakan adalah library research dengan pendekatan kualitatif deskriptif. Data diperoleh dari artikel ilmiah, buku, prosiding, peraturan, dan dokumen resmi yang relevan, kemudian dianalisis menggunakan teknik content analysis. Hasil kajian menunjukkan bahwa tekanan publikasi, rendahnya pemahaman etika penelitian, lemahnya pengawasan institusi, dan budaya akademik yang permisif menjadi faktor utama terjadinya fabrikasi riset. Praktik tersebut berdampak pada menurunnya kualitas penelitian, rusaknya reputasi akademisi dan institusi, serta berkurangnya kepercayaan publik terhadap hasil penelitian. Oleh karena itu, penguatan etika penelitian, penerapan kebijakan integritas akademik, edukasi berkelanjutan, serta pengawasan yang konsisten menjadi langkah strategis untuk mencegah fabrikasi riset dan membangun budaya akademik yang jujur, transparan, serta bertanggung jawab.