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mRNA Relative Expression Catalase in Hypertension Yohana, Yohana; Faradilla, Meutia Atika; Meiyanti, Meiyanti; Hartanti, Monica Dwi; Margo, Eveline; Anastasya, Karina Shasri
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12825

Hypertension is number one worldwide disease which lead to death. Initially hypertension is caused by changes in the walls of blood vessels. These vascular changes are due to the accumulation of ROS (Reactive Oxygen Species). ROS is the result of the balance between antioxidants and oxidants, but uncontrolled conditions will cause vascular damage. One of ROS product from metabolism which could played role in downregulation antioxidant gene expression is hydrogen peroxide. Hydrogen peroxide could be neutralized by catalase enzyme. Studies about catalase expression in hypertension subject is still limited. This research aims to determine mRNA relative expression catalase in hypertension. In this case control study, thrity subject hypertension and normotension subject were recruited within the age of 50-60 years. Hypertension subject was chosen according to JNC 8. Two millilitres vein blood was isolated into RNA. mRNA expression was detected by qRT PCR with 2 steps. Relative expression was determined using livak method. The data was analyzed using Mann Withney test with GraphPad software. Research results show that (1) catalase mRNA relative expression was 0,6 fold in hypertension; (2) catalase relative expression was not significantly lower in hypertension; (3) other antioxidants might have other mechanism to detoxify ROS and prevent hypertension.

Increasing Knowledge About Stunting and Growth Charts Through Seminars and Assistance for Parents of Students Aged 3 – 18 Years Liman, Patricia Budihartanti; Dita Setiati; Yuliana; Faradilla, Meutia Atika; Anastasya, Karina Shasri; Yenny
International Journal of Community Service Learning Vol. 8 No. 4 (2024): November
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijcsl.v8i4.79886

Stunting and obesity in Indonesia are still unresolved problems This may be caused by a lack of parental knowledge. StResearch regarding increasing parental knowledge about stunting and the use of growth charts is still controversial. This research aims to determine the effectiveness of health education on parental knowledge. This was a one-group experimental pre- and post-test study involving 61 parents of children aged 3-18 years. A modified structured questionnaire was used to assess stunting knowledge scores through seminars and growth chart knowledge scores through mentoring. Data were analyzed using SPSS ver. 29.0.2.0. The Mann-Whitney test was used to compare changes in scores after the seminar and mentoring training. Post-test knowledge score of seminars and mentoring method improved significantly compared with pretest scores (p < 0.001 for both analyses). The increase in scores for the mentoring method was three times compared to the seminar method (p=0.016). The implication of these findings is that mentoring methods can be considered further on a larger community scale for parent capacity building programs to achieve reduction of malnutrition in Indonesia.

PERAN PENANDA LC3 SEBAGAI FUNGSI AUTOFAGI PADA AGING Xavierees, Endrico; Yohana, Yohana; Faradilla, Meutia Atika; Yastani, Deasyka; Anastasya, Karina Shasri; Suweino, Suweino
JURNAL PENELITIAN DAN KARYA ILMIAH LEMBAGA PENELITIAN UNIVERSITAS TRISAKTI Volume 10, Nomor 1, Januari 2025
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/pdk.v10i1.21848

Autofagi adalah proses kompleks yang masih perlu diteliti lebih dalam. Proses ini ditandai dengan adanya pembentukan membran oleh peran protein spesifik yang disebut LC3, yang memainkan peran penting dalam mekanisme autofagi. Autofagi adalah bagian penting dari proses metabolisme, karena melibatkan pergerakan organel dari limbah ke autofagosom. Proses ini difasilitasi oleh adanya fagofor dan isolasi membran, yang penting untuk pembentukan autofagosom. Autofagosom kemudian membentuk struktur kompleks dengan lisosom, yang kemudian diubah menjadi autolisosom dan bahan yang terdegradasi. Secara umum juga dikenal dengan makroautofagi namundapat ditemukan jenis lain seperti xenofagi, chaperon-mediated autofagi. Merupakan proses penting dalam menjaga keseimbangan organ-organ internal tubuh, Autofagi juga terlibat dalam pengaturan berbagai proses fisiologis, seperti sekresi insulin, pengaturan gula darah, dan respons imun. Aging mulai dihubungkan dengan autofagi dan masih memiliki banyak ruang yang perlu didalami. Memiliki peran penting dalam aging, kehamilan dan kasus kehamilan lain seperti hambatan pertumbuhan janin, kelahiran preterm, dan Diabetes Gestasional yang banyak ditemukan pada Wanita dengan usia lebih tinggi. Mulai banyak penelitian yang menghubungkan autofagi dengan bertambahnya usia. Hasil Penelitian sebelumnya menunjukkan hubungan erat sehingga mendorong penelitian lebih lanjut untuk menjelaskan fungsi Autofagi pada aging.

PENYULUHAN INTERVENSI GIZI IBU HAMIL DENGAN KURANG ENERGI KRONIK KECAMATAN CIAMBAR SUKABUMI Sudarma, Verawati; Liman, Patricia Budihartanti; Anastasya, Karina Shasri; Istriana, Erita; Zulkarnain, Salwa Fildzah; Primaresti, Arella Fina; Maizura, Fanny Izzati
Jurnal Pengabdian Masyarakat Trimedika Vol. 2 No. 1 (2025)
Publisher : Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/abdimastrimedika.v2i1.22056

Kekurangan Energi Kronis (KEK) pada ibu hamil merupakan salah satu bentuk masalah gizi yang timbul akibat asupan zat gizi yang tidak seimbang dalam jangka waktu yang cukup lama. Kondisi KEK pada wanita hamil dapat menimbulkan berbagai kompilkasi selama kehamilan juga efek samping pasca persalinan baik untuk bayi dan ibu. Pada ibu dapat terjadi anemia, pertambahan berat badan masa kehamilan yang tidak adekuat, perdarahan, bahkan kematian ibu. Selanjutnya pada bayi, dilaporkan ditemukan kondisi berat badan lahir rendah, gangguan pertumbuhan intra uterin, abnormalitas kehamilan, kelainan kongenital, kematian janin intrauterine dan juga stunting. Penapisan status gizi sebelum ibu masuk dalam masa kehamilan menjadi penting untuk dilakukan. Pengabdian kepada masyarakat dilakukan dalam bentuk penyuluhan kepada 26 ibu hamil dan 15 kader di Kecamatan Ciambar, Sukabumi. Selain itu dilakukan pengukuran lingkar lengan atas (LLA) dan pengisian kuesioner sebelum dan setelah penyuluhan. Hasil kegiatan ini didapatkan data bahwa usia ibu hamil yang mengikuti PkM antara 21 – 39 tahun, riwayat gestasi adalah 1 – 4, rerata LLA adalah 28,19 cm, dan terdapat terdapat peningkatan pengetahuan dari 9,5 saat pre-test menjadi 9,88 saat post-test. Penyuluhan tentang asupan nutrisi yang baik dapat menjadi salah satu tombak peningkatan pengetahuan bagi ibu hamil dan keluarganya. Penyediaan makanan yang bergizi namun mudah didapat, ekonomis, mudah dibuat dan sesuai kearifan lokal menjadi salah satu pendekatan dalam upaya pencegahan stunting selain mempertahankan status kesehatan ibu yang optimal.

mRNA Relative Expression Catalase in Hypertension Yohana, Yohana; Faradilla, Meutia Atika; Meiyanti, Meiyanti; Hartanti, Monica Dwi; Margo, Eveline; Anastasya, Karina Shasri
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12825

Hypertension is number one worldwide disease which lead to death. Initially hypertension is caused by changes in the walls of blood vessels. These vascular changes are due to the accumulation of ROS (Reactive Oxygen Species). ROS is the result of the balance between antioxidants and oxidants, but uncontrolled conditions will cause vascular damage. One of ROS product from metabolism which could played role in downregulation antioxidant gene expression is hydrogen peroxide. Hydrogen peroxide could be neutralized by catalase enzyme. Studies about catalase expression in hypertension subject is still limited. This research aims to determine mRNA relative expression catalase in hypertension. In this case control study, thrity subject hypertension and normotension subject were recruited within the age of 50-60 years. Hypertension subject was chosen according to JNC 8. Two millilitres vein blood was isolated into RNA. mRNA expression was detected by qRT PCR with 2 steps. Relative expression was determined using livak method. The data was analyzed using Mann Withney test with GraphPad software. Research results show that (1) catalase mRNA relative expression was 0,6 fold in hypertension; (2) catalase relative expression was not significantly lower in hypertension; (3) other antioxidants might have other mechanism to detoxify ROS and prevent hypertension.

Hubungan Profil Lipid dan Kejadian Migren pada Karyawan di Jakarta Satyasari, Daniella; Larasari, Andira; Faradilla, Meutia Atika; Anastasya, Karina Shasri
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14885

One of the risk factor for migraines is an elevated lipid profile. The most significant of the primary lipoproteins that causes atherosclerosis and readily binds to blood vessel walls is LDL. Because lipoprotein A (Lp(a)) levels have an atherogenic ApoA structure, increasing oxidative stress and fat oxidation can lead to endogenous vascular injury. Cross-sectional design was used to perform this study from October 2023 to June 2024 in Universitas Trisakti, Faculty of Medicine. The purpose of this study is to evaluate the association between the lipid level and the incidence of migraines among employees in Jakarta. The target population consists of Jakarta's private employees. With a total sample size of 66 employees that meet the inclusion and exclusion criteria, the study sample will be drawn using the sequential sampling method. The study's findings highlighted that high LDL (p=0.002) and total cholesterol (p=0.0001) had a significant relationship with migraines, whereas HDL, triglycerides, and Lp(a) had an insignificant link with the incidence of migraine (p>0.05). The study concluded that LDL is a powerful predictor of migraine occurrence and has a significant association with migraine.

Endometriosis pada Luka Operasi Seksio Sesarea: Suatu Laporan Kasus Yastani, Deasyka; Wirawan, Jimmy Panji; Anastasya, Karina Shasri; Faradilla, Meutia Atika; Xavierees, Endrico; Yohana, Yohana; Suweino, Suweino; Rahmansyah, Mulia; Mahardieni, Karlina; Ferina, Mutiara
Jurnal Biomedika dan Kesehatan Vol 8 No 3 (2025)
Publisher : Fakultas Kedokteran Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/JBiomedKes.2025.v8.329-333

Caesarean scar endometriosis is a rare form of extra pelvic extrapelvic or extra-pelvic endometriosis that often presents with nonspecific symptoms, leading to diagnostic challenges. We report the case of a 41-year-old woman who presented with a painful subcutaneous mass at the site of a previous caesarean section, noted eight months post-surgery. MRI imaging revealed a mass suggestive of cutaneous endometriosis, and a wide excision was performed. Histopathological analysis confirmed the diagnosis by identifying endometrial glands and stroma within the cutaneous tissue. We report the case of a 41-year-old woman with a painful subcutaneous mass at the site of a previous cesarean section. The mass was detected eight months after the cesarean section. Following magnetic resonance imaging (MRI), which demonstrated a mass characterized as cutaneous endometriosis, the patient underwent wide excision. Histopathological analysis confirmed the diagnosis by demonstrating endometrial glands and stroma within the cutaneous tissue. Caesarean scar endometriosis is often associated with Pfannenstiel incisions, with a latency period of symptom onset averaging over two years. While imaging, such as MRI, is useful for preoperative assessment, surgical excision remains the definitive treatment. Recurrence is uncommon but can occur. Iatrogenic seeding of endometrial cells during surgery is a likely etiological factor, highlighting the importance of proper surgical technique and wound cleaning. Cesarean scar endometriosis is often associated with a Pfannenstiel incision, with an average latency period of more than two years. Although imaging such as MRI is useful for preoperative assessment, surgical excision remains the definitive treatment. Recurrence is rare but can occur. Iatrogenic endometrial cell seeding during surgery is a likely etiologic factor, highlighting the importance of proper surgical technique and wound cleansing. The conclusion was although rare, cutaneous endometriosis should be considered in women presenting with cyclical pain and masses at surgical scars post-caesarean - cyclic pain and masses in their post-cesarean scars. Early recognition and surgical intervention are key to effective management and prevention of cutaneous endometrosis recurrence.

Serum 25-hydroxyvitamin D is associated with body fat percentage and skeletal muscle mass in adults: A cross-sectional study Faradilla, Meutia Atika; Yastani, Deasyka; Yohana, Yohana; Anastasya, Karina Shasri; Salma, Nabila Maudy
Science Midwifery Vol 13 No 6 (2026): February: Health Sciences and related fields
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v13i6.2253

Background: Vitamin D is widely recognized for its role in bone and musculoskeletal health, yet accumulating evidence suggests it may also influence body composition through regulatory effects on adipose and skeletal muscle tissues. This study aimed to evaluate the association between serum vitamin D status and body composition parameters in adults. Methods: A cross-sectional study was conducted in 77 adults. Serum 25-hydroxyvitamin D [25(OH)D] concentrations were measured using an enzyme-linked immunosorbent assay (ELISA), while body composition (percent body fat and skeletal muscle mass) was assessed using bioelectrical impedance analysis (BIA). Participants were categorized based on vitamin D status, and group comparisons as well as Pearson correlation analyses were performed. Results: The mean age of participants was 35.8 ± 9.6 years, and 40.2% were men. The prevalence of vitamin D deficiency (<20 ng/mL) was 36%, while 68% of participants had suboptimal vitamin D levels (deficiency or insufficiency). Individuals with vitamin D deficiency showed significantly higher body fat percentage and lower skeletal muscle mass compared with the non-deficient group (p < 0.05). Serum 25(OH)D was moderately inversely correlated with body fat percentage (r = −0.41, p = 0.018) and positively correlated with skeletal muscle mass (r = 0.29, p = 0.012). Conclusion: Lower vitamin D status was associated with increased adiposity and reduced skeletal muscle mass in adults. These findings highlight the potential importance of maintaining adequate vitamin D levels as part of strategies supporting healthier body composition. Longitudinal and interventional studies are needed to clarify causality and clinical implications.

The Relationship Between Nutritional Status with Malondialdehyde (MDA) and Vitamin D Level Anastasya, Karina Shasri; Faradilla, Meutia Atika; Satyasari, Daniella; Larasari, Andira
The Avicenna Medical Journal Vol. 6 No. 2 (2025): The Avicenna Medical Journal
Publisher : Faculty of Medicine, UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/avicenna.v6i2.47151

Background: Nutritional status has been found to be correlated with malondialdehyde (MDA) profiles in the blood. MDA is a marker of oxidative stress in the body and is the final product of lipid peroxidation chain reactions. The long-term undernutrition and excess status will be closely related to metabolic disorders and degenerative diseases. Street cleaning staff are often exposed to free radicals that will improve lipid peroxidation, which will also break down into MDA. Vitamin D serum levels have an inverse correlation with adipose tissue mass, where the higher the BMI and body fat mass of a person, the lower the levels of vitamin D serum. The purpose of this study was to determine the relationship of nutritional status with MDA and vitamin D levels in the blood. Methods: This research will be conducted from July 2024 to February 2025, using a cross-sectional design at the Faculty of Medicine, Trisakti University, with the target population being the street cleaning staff in Jakarta. The samples were taken by consecutive sampling for all street cleaning staff who met the inclusion and exclusion criteria, with a total of 76 samples. Results: The results showed that there was no significant relationship between nutritional status with MDA and vitamin D, but there was a significant relationship between vitamin D and MDA (r=0.190; p=0.047). Conclusion: Higher BMI is associated with a relative reduction in muscle mass percentage and a substantial increase in visceral adiposity, irrespective of sex. These findings suggest that BMI alone may not adequately represent body composition or metabolic risk and support the routine use of body composition assessment as a complementary tool in clinical obesity evaluation. Keywords: Nutritional status, MDA, vitamin D

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