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mRNA Relative Expression Catalase in Hypertension Yohana, Yohana; Faradilla, Meutia Atika; Meiyanti, Meiyanti; Hartanti, Monica Dwi; Margo, Eveline; Anastasya, Karina Shasri
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12825

Hypertension is number one worldwide disease which lead to death. Initially hypertension is caused by changes in the walls of blood vessels. These vascular changes are due to the accumulation of ROS (Reactive Oxygen Species). ROS is the result of the balance between antioxidants and oxidants, but uncontrolled conditions will cause vascular damage. One of ROS product from metabolism which could played role in downregulation antioxidant gene expression is hydrogen peroxide. Hydrogen peroxide could be neutralized by catalase enzyme. Studies about catalase expression in hypertension subject is still limited. This research aims to determine mRNA relative expression catalase in hypertension. In this case control study, thrity subject hypertension and normotension subject were recruited within the age of 50-60 years. Hypertension subject was chosen according to JNC 8. Two millilitres vein blood was isolated into RNA. mRNA expression was detected by qRT PCR with 2 steps. Relative expression was determined using livak method. The data was analyzed using Mann Withney test with GraphPad software. Research results show that (1) catalase mRNA relative expression was 0,6 fold in hypertension; (2) catalase relative expression was not significantly lower in hypertension; (3) other antioxidants might have other mechanism to detoxify ROS and prevent hypertension.

Increasing Knowledge About Stunting and Growth Charts Through Seminars and Assistance for Parents of Students Aged 3 – 18 Years Liman, Patricia Budihartanti; Dita Setiati; Yuliana; Faradilla, Meutia Atika; Anastasya, Karina Shasri; Yenny
International Journal of Community Service Learning Vol. 8 No. 4 (2024): November
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijcsl.v8i4.79886

Stunting and obesity in Indonesia are still unresolved problems This may be caused by a lack of parental knowledge. StResearch regarding increasing parental knowledge about stunting and the use of growth charts is still controversial. This research aims to determine the effectiveness of health education on parental knowledge. This was a one-group experimental pre- and post-test study involving 61 parents of children aged 3-18 years. A modified structured questionnaire was used to assess stunting knowledge scores through seminars and growth chart knowledge scores through mentoring. Data were analyzed using SPSS ver. 29.0.2.0. The Mann-Whitney test was used to compare changes in scores after the seminar and mentoring training. Post-test knowledge score of seminars and mentoring method improved significantly compared with pretest scores (p < 0.001 for both analyses). The increase in scores for the mentoring method was three times compared to the seminar method (p=0.016). The implication of these findings is that mentoring methods can be considered further on a larger community scale for parent capacity building programs to achieve reduction of malnutrition in Indonesia.

PERAN PENANDA LC3 SEBAGAI FUNGSI AUTOFAGI PADA AGING Xavierees, Endrico; Yohana, Yohana; Faradilla, Meutia Atika; Yastani, Deasyka; Anastasya, Karina Shasri; Suweino, Suweino
JURNAL PENELITIAN DAN KARYA ILMIAH LEMBAGA PENELITIAN UNIVERSITAS TRISAKTI Volume 10, Nomor 1, Januari 2025
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/pdk.v10i1.21848

Autofagi adalah proses kompleks yang masih perlu diteliti lebih dalam. Proses ini ditandai dengan adanya pembentukan membran oleh peran protein spesifik yang disebut LC3, yang memainkan peran penting dalam mekanisme autofagi. Autofagi adalah bagian penting dari proses metabolisme, karena melibatkan pergerakan organel dari limbah ke autofagosom. Proses ini difasilitasi oleh adanya fagofor dan isolasi membran, yang penting untuk pembentukan autofagosom. Autofagosom kemudian membentuk struktur kompleks dengan lisosom, yang kemudian diubah menjadi autolisosom dan bahan yang terdegradasi. Secara umum juga dikenal dengan makroautofagi namundapat ditemukan jenis lain seperti xenofagi, chaperon-mediated autofagi. Merupakan proses penting dalam menjaga keseimbangan organ-organ internal tubuh, Autofagi juga terlibat dalam pengaturan berbagai proses fisiologis, seperti sekresi insulin, pengaturan gula darah, dan respons imun. Aging mulai dihubungkan dengan autofagi dan masih memiliki banyak ruang yang perlu didalami. Memiliki peran penting dalam aging, kehamilan dan kasus kehamilan lain seperti hambatan pertumbuhan janin, kelahiran preterm, dan Diabetes Gestasional yang banyak ditemukan pada Wanita dengan usia lebih tinggi. Mulai banyak penelitian yang menghubungkan autofagi dengan bertambahnya usia. Hasil Penelitian sebelumnya menunjukkan hubungan erat sehingga mendorong penelitian lebih lanjut untuk menjelaskan fungsi Autofagi pada aging.

PENYULUHAN INTERVENSI GIZI IBU HAMIL DENGAN KURANG ENERGI KRONIK KECAMATAN CIAMBAR SUKABUMI Sudarma, Verawati; Liman, Patricia Budihartanti; Anastasya, Karina Shasri; Istriana, Erita; Zulkarnain, Salwa Fildzah; Primaresti, Arella Fina; Maizura, Fanny Izzati
Jurnal Pengabdian Masyarakat Trimedika Vol. 2 No. 1 (2025)
Publisher : Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/abdimastrimedika.v2i1.22056

Kekurangan Energi Kronis (KEK) pada ibu hamil merupakan salah satu bentuk masalah gizi yang timbul akibat asupan zat gizi yang tidak seimbang dalam jangka waktu yang cukup lama. Kondisi KEK pada wanita hamil dapat menimbulkan berbagai kompilkasi selama kehamilan juga efek samping pasca persalinan baik untuk bayi dan ibu. Pada ibu dapat terjadi anemia, pertambahan berat badan masa kehamilan yang tidak adekuat, perdarahan, bahkan kematian ibu. Selanjutnya pada bayi, dilaporkan ditemukan kondisi berat badan lahir rendah, gangguan pertumbuhan intra uterin, abnormalitas kehamilan, kelainan kongenital, kematian janin intrauterine dan juga stunting. Penapisan status gizi sebelum ibu masuk dalam masa kehamilan menjadi penting untuk dilakukan. Pengabdian kepada masyarakat dilakukan dalam bentuk penyuluhan kepada 26 ibu hamil dan 15 kader di Kecamatan Ciambar, Sukabumi. Selain itu dilakukan pengukuran lingkar lengan atas (LLA) dan pengisian kuesioner sebelum dan setelah penyuluhan. Hasil kegiatan ini didapatkan data bahwa usia ibu hamil yang mengikuti PkM antara 21 – 39 tahun, riwayat gestasi adalah 1 – 4, rerata LLA adalah 28,19 cm, dan terdapat terdapat peningkatan pengetahuan dari 9,5 saat pre-test menjadi 9,88 saat post-test. Penyuluhan tentang asupan nutrisi yang baik dapat menjadi salah satu tombak peningkatan pengetahuan bagi ibu hamil dan keluarganya. Penyediaan makanan yang bergizi namun mudah didapat, ekonomis, mudah dibuat dan sesuai kearifan lokal menjadi salah satu pendekatan dalam upaya pencegahan stunting selain mempertahankan status kesehatan ibu yang optimal.

APOA2–265 T>C Polymorphism as A Genetic Marker Associated with Lipid Profiles and Cardiovascular Risk in A Healthy Indonesian Population Hartanti, Monica Dwi; Hizbulloh, Ilham; Satibi, Janice Puteri; Virgo, Ricardo Lie; Anastasya, Karina Shasri; Adli, Mizanul; Amarendra, Gerie; Junnata, Agung
The Indonesian Biomedical Journal Vol 17, No 3 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i3.3472

BACKGROUND: Apolipoprotein A (APOA)2–265 T>C polymorphism significantly affects lipid metabolism and body composition, as well as plays a key role in cardiovascular diseases (CVD) and metabolic syndrome. In this study, association between the APOA2 polymorphism, lipid profiles, body composition, and cardiovascular disease (CVD) risk in a healthy Indonesian population was investigated. Although similar studies have been conducted in other populations, this study addresses the urgent need to understand genetic factors influencing lipid profiles in Southeast and East Asia, where hypercholesterolemia rate keep rising, particularly in Indonesia.METHODS: A cross-sectional study involving 84 healthy participants was performed. Genotyping for the APOA2–265 T>C polymorphism was conducted using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Plasma levels of APOA2 and APOB100 were measured with enzyme-linked immunosorbent assay (ELISA), and APOB100/APOA2 ratio was calculated to assess CVD risk. Lipid profiles were evaluated with enzymatic methods, and body fat percentage was measured using calipers.RESULTS: CT/CC genotypes showed significantly lower plasma APOA2 levels compared to the TT genotype (p=0.0215). APOB100/APOA2 ratio was significantly higher in CT/CC genotypes (p=0.0020) and remained significant after Bonferroni correction. No significant differences were found in lipid profiles and body fat percentages between genotypes after correction, although trends suggested higher cholesterol and low-density lipoprotein (LDL) levels in TT genotypes and higher median body fat percentages in CC/CT genotypes.CONCLUSION: APOA2–265 T>C polymorphism is linked to changes in lipid profiles and body composition, potentially raising CVD risk in CT/CC genotypes. However, limited sample size and modest effect sizes suggest that the practical use of APOA2 genotyping for risk assessment might require further investigation.KEYWORDS: APOA2 polymorphism, APOB100, hypercholesterolemia, cardiovascular disease, lipid profile, body fat percentage

mRNA Relative Expression Catalase in Hypertension Yohana, Yohana; Faradilla, Meutia Atika; Meiyanti, Meiyanti; Hartanti, Monica Dwi; Margo, Eveline; Anastasya, Karina Shasri
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12825

Hypertension is number one worldwide disease which lead to death. Initially hypertension is caused by changes in the walls of blood vessels. These vascular changes are due to the accumulation of ROS (Reactive Oxygen Species). ROS is the result of the balance between antioxidants and oxidants, but uncontrolled conditions will cause vascular damage. One of ROS product from metabolism which could played role in downregulation antioxidant gene expression is hydrogen peroxide. Hydrogen peroxide could be neutralized by catalase enzyme. Studies about catalase expression in hypertension subject is still limited. This research aims to determine mRNA relative expression catalase in hypertension. In this case control study, thrity subject hypertension and normotension subject were recruited within the age of 50-60 years. Hypertension subject was chosen according to JNC 8. Two millilitres vein blood was isolated into RNA. mRNA expression was detected by qRT PCR with 2 steps. Relative expression was determined using livak method. The data was analyzed using Mann Withney test with GraphPad software. Research results show that (1) catalase mRNA relative expression was 0,6 fold in hypertension; (2) catalase relative expression was not significantly lower in hypertension; (3) other antioxidants might have other mechanism to detoxify ROS and prevent hypertension.

Hubungan Profil Lipid dan Kejadian Migren pada Karyawan di Jakarta Satyasari, Daniella; Larasari, Andira; Faradilla, Meutia Atika; Anastasya, Karina Shasri
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14885

One of the risk factor for migraines is an elevated lipid profile. The most significant of the primary lipoproteins that causes atherosclerosis and readily binds to blood vessel walls is LDL. Because lipoprotein A (Lp(a)) levels have an atherogenic ApoA structure, increasing oxidative stress and fat oxidation can lead to endogenous vascular injury. Cross-sectional design was used to perform this study from October 2023 to June 2024 in Universitas Trisakti, Faculty of Medicine. The purpose of this study is to evaluate the association between the lipid level and the incidence of migraines among employees in Jakarta. The target population consists of Jakarta's private employees. With a total sample size of 66 employees that meet the inclusion and exclusion criteria, the study sample will be drawn using the sequential sampling method. The study's findings highlighted that high LDL (p=0.002) and total cholesterol (p=0.0001) had a significant relationship with migraines, whereas HDL, triglycerides, and Lp(a) had an insignificant link with the incidence of migraine (p>0.05). The study concluded that LDL is a powerful predictor of migraine occurrence and has a significant association with migraine.

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